FastQCFastQC Report
Sat 18 Jun 2016
SRR3550605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654052
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40130.6135597781216172No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7800.11925657287188175No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTG207.0310535E-445.00000444
TACTACG207.0310535E-445.0000041
TAACGCC253.8889557E-545.012
TAGCCGT650.041.53846444
TACGAAT1100.040.90909212
CGGGTAC501.0804797E-940.56
CGTTTTT22700.040.0440521
CGTTAGG900.040.02
TACGAAA800.039.37500420
TCGACGG403.4551704E-739.3750041
CGAATAT1100.038.8636414
TCGTTAG356.2441613E-638.5714261
TTAGGCG301.1393842E-437.4999961
GCGTTAG601.546141E-1037.4999961
CGTAGTG301.1393842E-437.4999962
CTTGACG301.1393842E-437.4999961
TATTACG301.1393842E-437.4999961
GACACGA2050.037.3170725
CACGACC2050.037.3170727
ACGACCA2050.037.3170728
Produced by FastQC (version 0.11.5)