##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550605_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	654052
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31494590644169	33.0	31.0	34.0	31.0	34.0
2	32.46095570382783	34.0	31.0	34.0	31.0	34.0
3	32.40635454061756	34.0	31.0	34.0	30.0	34.0
4	35.9665317130748	37.0	35.0	37.0	35.0	37.0
5	36.07917566187398	37.0	35.0	37.0	35.0	37.0
6	36.114897592240375	37.0	36.0	37.0	35.0	37.0
7	36.216759523707594	37.0	37.0	37.0	35.0	37.0
8	36.22143805079718	37.0	37.0	37.0	35.0	37.0
9	38.038122351128045	39.0	39.0	39.0	37.0	39.0
10	37.69173551949998	39.0	38.0	39.0	35.0	39.0
11	37.560452379933096	39.0	37.0	39.0	35.0	39.0
12	37.40433788139169	39.0	37.0	39.0	35.0	39.0
13	37.39472243797129	39.0	37.0	39.0	35.0	39.0
14	38.63793551583055	40.0	38.0	41.0	35.0	41.0
15	38.732265936041784	40.0	38.0	41.0	35.0	41.0
16	38.75821647208479	40.0	38.0	41.0	35.0	41.0
17	38.68969439738736	40.0	38.0	41.0	35.0	41.0
18	38.58819482243002	40.0	38.0	41.0	35.0	41.0
19	38.49856280540385	40.0	37.0	41.0	34.0	41.0
20	38.419286234122055	40.0	37.0	41.0	34.0	41.0
21	38.345250836324944	40.0	37.0	41.0	34.0	41.0
22	38.359044846587125	40.0	37.0	41.0	34.0	41.0
23	38.33283745023331	40.0	36.0	41.0	34.0	41.0
24	38.247209701980886	40.0	36.0	41.0	34.0	41.0
25	38.16707845859351	40.0	36.0	41.0	34.0	41.0
26	38.18762269666632	40.0	36.0	41.0	34.0	41.0
27	38.1461779797325	40.0	36.0	41.0	34.0	41.0
28	38.07918483545651	40.0	36.0	41.0	34.0	41.0
29	38.03645887482953	40.0	36.0	41.0	34.0	41.0
30	37.89939943613046	40.0	36.0	41.0	34.0	41.0
31	37.822254193856146	40.0	35.0	41.0	34.0	41.0
32	37.650436050956195	40.0	35.0	41.0	33.0	41.0
33	37.51251582442986	40.0	35.0	41.0	33.0	41.0
34	37.36369891078997	40.0	35.0	41.0	33.0	41.0
35	37.29244005063818	40.0	35.0	41.0	33.0	41.0
36	37.22910563686068	40.0	35.0	41.0	33.0	41.0
37	37.203821714481414	40.0	35.0	41.0	33.0	41.0
38	37.13348327044333	40.0	35.0	41.0	33.0	41.0
39	37.07828276650786	40.0	35.0	41.0	33.0	41.0
40	36.99602325197385	40.0	35.0	41.0	32.0	41.0
41	36.902672876162754	40.0	35.0	41.0	32.0	41.0
42	36.85711686532569	39.0	35.0	41.0	32.0	41.0
43	36.769279812614286	39.0	35.0	41.0	32.0	41.0
44	36.69000171240207	39.0	35.0	41.0	32.0	41.0
45	36.60344743231425	39.0	35.0	41.0	31.0	41.0
46	36.48684814051482	39.0	35.0	41.0	31.0	41.0
47	36.43489355586406	39.0	35.0	41.0	31.0	41.0
48	36.38052479007785	39.0	35.0	41.0	31.0	41.0
49	36.32950896870585	39.0	35.0	41.0	31.0	41.0
50	36.23915682545119	39.0	35.0	41.0	31.0	41.0
51	35.379575324286144	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	4.0
8	4.0
9	17.0
10	15.0
11	12.0
12	9.0
13	11.0
14	22.0
15	29.0
16	43.0
17	86.0
18	154.0
19	259.0
20	404.0
21	668.0
22	1014.0
23	1552.0
24	2415.0
25	3607.0
26	5308.0
27	6455.0
28	6836.0
29	7285.0
30	8333.0
31	9775.0
32	12388.0
33	17315.0
34	38856.0
35	56066.0
36	40359.0
37	59138.0
38	113209.0
39	262092.0
40	311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.84766960425165	20.534300025074458	25.461889880315326	13.156140490358565
2	33.93751567153682	23.833426088445567	25.556071994275687	16.672986245741928
3	32.087968540727644	23.224147315503966	28.5159283971305	16.17195574663788
4	27.844727942120812	25.61508870854305	28.204026591157888	18.336156758178248
5	25.195244414817168	30.285512466898656	26.46792609761915	18.05131702066502
6	27.40271415728413	33.19491416584614	24.860102866438755	14.542268810430976
7	81.58525621816003	5.559496798419698	8.906325490939558	3.9489214924807206
8	84.20324377878211	4.4450594142361775	8.221058876052668	3.1306379309290393
9	78.20922495459077	7.0645759052797015	9.843712732320977	4.882486407808553
10	43.174548812632636	27.556218771596143	16.557857785007922	12.711374630763302
11	32.27709723385908	23.4097594686661	26.332309969237922	17.980833328236898
12	29.51997700488646	22.561050191727876	29.000752233767347	18.91822056961832
13	26.096243112168448	22.694067138392665	30.13690042993524	21.07278931950365
14	22.287524539333265	24.61975500418927	31.969935112192914	21.12278534428455
15	23.353189043073026	24.050075529162818	32.824454324732585	19.772281103031563
16	27.865980074978747	23.99060013576902	30.090115159039343	18.05330463021289
17	25.878370527114054	24.499886859148816	29.5609523401809	20.060790273556233
18	26.833799147468397	24.34622935179466	29.34613761596937	19.473833884767572
19	25.8377009779039	27.17551509665898	27.603004042492035	19.38377988294509
20	27.88952560346884	26.47694678710561	27.684190247870198	17.94933736155535
21	27.174444845363976	27.070783362790724	27.713239925877453	18.041531865967844
22	25.436203849235227	23.92913713282736	29.55407215328445	21.080586864652965
23	25.342939093527733	23.968889323784655	29.841052393387677	20.847119189299935
24	25.92255661629351	23.519842459009375	29.76751084011669	20.79009008458043
25	24.178964363689737	24.6434534257215	28.102199825090356	23.0753823854984
26	22.27269391424535	25.980350186223728	29.342621076000075	22.40433482353085
27	21.97745744986637	25.175826998464956	29.883250873019268	22.9634646786494
28	21.06774384911291	27.152122461211036	29.551320078525865	22.228813611150184
29	24.26901836551222	25.25823634818027	29.121231950976373	21.351513335331134
30	24.93731385272119	24.444845363977176	29.865973959257065	20.75186682404457
31	26.145321778696495	25.246310690893083	28.069939393198094	20.538428137212332
32	26.71515414676509	25.34538538220203	27.912153773706066	20.027306697326818
33	26.801538715576136	25.195855986985745	27.496437592118056	20.506167705320067
34	23.506999443469326	26.32879342926862	30.03385051953056	20.130356607731496
35	23.1475479013901	28.070398072324526	28.653532135059596	20.128521891225773
36	23.238825047549735	30.02177196920123	26.845419018671297	19.893983964577743
37	24.81897463810217	28.875838618336157	26.342706696103672	19.962480047458
38	23.92653795111092	28.996624121629473	25.6617210863968	21.415116840862805
39	25.2330089962266	26.368086941099484	26.626476182321895	21.772427880352023
40	24.366564126399737	24.87630952890596	27.310978331998065	23.446148012696238
41	21.608985218300685	27.028431990116992	27.709876278950297	23.652706512632022
42	22.1771357629057	25.89151932873839	28.04761700904515	23.88372789931076
43	22.906129787845614	25.10213255215182	27.99548048167424	23.996257178328328
44	22.65003394225536	25.225975916288	27.66324390109655	24.46074624036009
45	23.864922055127117	25.006115721685738	27.22382929797631	23.90513292521084
46	22.999547436595254	26.56134374636879	27.56646260541975	22.872646211616203
47	21.89565967231963	25.813238091160944	28.896173392941233	23.394928843578185
48	21.890767094971043	25.41434014420872	30.383669799954742	22.3112229608655
49	23.674876003742824	24.6202136833157	29.330083846544312	22.374826466397167
50	21.41618709215781	24.718676802456073	29.339869001241492	24.525267104144625
51	20.8118008965648	24.754606667359784	29.885697161693564	24.547895274381855
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	339.0
1	834.5
2	1330.0
3	4584.5
4	7839.0
5	5149.0
6	2459.0
7	2370.0
8	2281.0
9	2331.0
10	2381.0
11	2312.0
12	2243.0
13	2320.0
14	2397.0
15	2344.0
16	2291.0
17	2198.5
18	2106.0
19	2085.5
20	2065.0
21	2267.5
22	2470.0
23	2710.0
24	2950.0
25	3477.5
26	4610.5
27	5216.0
28	6634.0
29	8052.0
30	9265.0
31	10478.0
32	12280.5
33	14083.0
34	15546.5
35	17010.0
36	18278.5
37	19547.0
38	21338.5
39	23130.0
40	25722.5
41	28315.0
42	30425.5
43	32536.0
44	34057.0
45	35578.0
46	38928.0
47	42278.0
48	45426.0
49	48574.0
50	49387.5
51	50201.0
52	48432.0
53	46663.0
54	44591.5
55	42520.0
56	40632.5
57	38745.0
58	37628.5
59	36512.0
60	35710.5
61	34909.0
62	31568.0
63	28227.0
64	23902.0
65	19577.0
66	16091.0
67	12605.0
68	10271.5
69	7938.0
70	6581.5
71	5225.0
72	4534.5
73	3844.0
74	3104.5
75	1861.0
76	1357.0
77	1036.0
78	715.0
79	548.5
80	382.0
81	289.0
82	196.0
83	136.0
84	76.0
85	57.0
86	38.0
87	20.5
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	654052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.573445097104457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.55991214425728	21.162731329518113
2	7.335897050734531	4.338954973358164
3	2.9473198495163606	2.614872052598348
4	1.8949140051095492	2.2415654117536628
5	1.5107640903460653	2.233924944026316
6	1.3024234760895177	2.3110289497987977
7	1.21379437054586	2.5127256823558715
8	1.0569561256394961	2.5006267161318303
9	0.9738526997676595	2.5920161414321248
>10	10.122953086531355	54.41884204437039
>50	0.06052186542102336	1.1640079528640563
>100	0.01810483153613125	0.9019570101280863
>500	0.002069123604129285	0.39284694742349485
>1k	5.172809010323213E-4	0.613899844240843
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4013	0.6135597781216172	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	780	0.11925657287188175	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.586791264303144E-4	0.0	0.0	0.09708708176108322	0.0
2	4.586791264303144E-4	0.0	0.0	0.30777369383474096	0.0
3	4.586791264303144E-4	0.0	0.0	0.4372741005302331	0.0
4	4.586791264303144E-4	0.0	0.0	0.5897084635472409	0.0
5	4.586791264303144E-4	0.0	0.0	1.0278999223303347	0.0
6	4.586791264303144E-4	0.0	0.0	1.3893390739574223	0.0
7	4.586791264303144E-4	0.0	0.0	1.7656088506724237	0.0
8	6.115721685737525E-4	0.0	0.0	2.2956890277837236	0.0
9	6.115721685737525E-4	0.0	0.0	2.59168995737342	0.0
10	6.115721685737525E-4	0.0	0.0	3.0977659268682003	0.0
11	6.115721685737525E-4	0.0	0.0	3.823549197923101	0.0
12	6.115721685737525E-4	0.0	0.0	4.376410438313774	0.0
13	7.644652107171906E-4	0.0	0.0	4.606820252823934	0.0
14	7.644652107171906E-4	0.0	0.0	4.724547895274382	0.0
15	7.644652107171906E-4	0.0	0.0	4.90190382416077	0.0
16	7.644652107171906E-4	0.0	0.0	5.1965287163711755	0.0
17	7.644652107171906E-4	0.0	0.0	5.57692660522405	0.0
18	9.173582528606288E-4	0.0	0.0	5.965122039226239	0.0
19	9.173582528606288E-4	0.0	0.0	6.256383284509488	0.0
20	9.173582528606288E-4	0.0	0.0	6.547950315877025	0.0
21	9.173582528606288E-4	0.0	0.0	6.882021612960437	0.0
22	9.173582528606288E-4	0.0	0.0	7.249882272357549	0.0
23	9.173582528606288E-4	0.0	0.0	7.6140734987432195	0.0
24	9.173582528606288E-4	0.0	0.0	7.926586876884406	0.0
25	9.173582528606288E-4	0.0	0.0	8.188033978949687	0.0
26	9.173582528606288E-4	0.0	0.0	8.445200075834949	0.0
27	9.173582528606288E-4	0.0	0.0	8.681419825946561	0.0
28	9.173582528606288E-4	0.0	0.0	8.94867686361329	0.0
29	9.173582528606288E-4	0.0	0.0	9.228929809862214	0.0
30	9.173582528606288E-4	0.0	0.0	9.53960847149768	0.0
31	9.173582528606288E-4	0.0	0.0	9.836985438466666	0.0
32	9.173582528606288E-4	0.0	0.0	10.134056619351366	0.0
33	9.173582528606288E-4	0.0	0.0	10.43036333502535	0.0
34	9.173582528606288E-4	0.0	0.0	10.730033697626489	0.0
35	9.173582528606288E-4	0.0	0.0	11.043311540978394	0.0
36	9.173582528606288E-4	0.0	0.0	11.373101832881789	0.0
37	0.001070251295004067	0.0	0.0	11.688061499697271	0.0
38	0.001070251295004067	0.0	0.0	12.089864414450227	0.0
39	0.001070251295004067	0.0	0.0	12.502828521279653	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTG	20	7.0310535E-4	45.000004	44
TACTACG	20	7.0310535E-4	45.000004	1
TAACGCC	25	3.8889557E-5	45.0	12
TAGCCGT	65	0.0	41.538464	44
TACGAAT	110	0.0	40.909092	12
CGGGTAC	50	1.0804797E-9	40.5	6
CGTTTTT	2270	0.0	40.044052	1
CGTTAGG	90	0.0	40.0	2
TACGAAA	80	0.0	39.375004	20
TCGACGG	40	3.4551704E-7	39.375004	1
CGAATAT	110	0.0	38.86364	14
TCGTTAG	35	6.2441613E-6	38.571426	1
TTAGGCG	30	1.1393842E-4	37.499996	1
GCGTTAG	60	1.546141E-10	37.499996	1
CGTAGTG	30	1.1393842E-4	37.499996	2
CTTGACG	30	1.1393842E-4	37.499996	1
TATTACG	30	1.1393842E-4	37.499996	1
GACACGA	205	0.0	37.31707	25
CACGACC	205	0.0	37.31707	27
ACGACCA	205	0.0	37.31707	28
>>END_MODULE