FastQCFastQC Report
Sat 18 Jun 2016
SRR3550603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516911
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53751.0398308412860242No Hit
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC12120.23446976365370442No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCC11470.22189506510791993No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT9060.17527195203816517TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC6470.12516661475573165No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT6460.12497315785502726No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTC5510.1065947522881115No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG207.0296804E-445.021
TAGACGT207.0296804E-445.01
ATTACGG207.0296804E-445.02
TTGTCGT253.887817E-545.012
TGTTACG207.0296804E-445.01
CGGTACA207.0296804E-445.06
TAAGACG207.0296804E-445.01
GCGTATG207.0296804E-445.01
CGTTTTT38200.042.702881
ATTAGCG501.0786607E-940.51
CTCACGA451.9248546E-840.024
TCGGGCA451.9248546E-840.05
CGACGGT403.4532968E-739.37528
TTCACGG403.4532968E-739.3752
TTAACGG403.4532968E-739.3752
CGGTCTA403.4532968E-739.37531
TAGGGTC750.039.05
TATCGGG356.2416184E-638.571433
ATCGCCA356.2416184E-638.5714324
TACACGG356.2416184E-638.571432