##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550603_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	516911
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14725165454014	33.0	31.0	34.0	30.0	34.0
2	32.29909597590301	33.0	31.0	34.0	30.0	34.0
3	32.201259017509784	33.0	31.0	34.0	30.0	34.0
4	35.84250480256756	37.0	35.0	37.0	35.0	37.0
5	35.975508356370824	37.0	35.0	37.0	35.0	37.0
6	36.02486114630952	37.0	35.0	37.0	35.0	37.0
7	36.13829073089952	37.0	36.0	37.0	35.0	37.0
8	36.13270176103817	37.0	37.0	37.0	35.0	37.0
9	37.94804328017783	39.0	38.0	39.0	35.0	39.0
10	37.55840754017616	39.0	37.0	39.0	35.0	39.0
11	37.526972728380706	39.0	37.0	39.0	35.0	39.0
12	37.449644136031154	39.0	37.0	39.0	35.0	39.0
13	37.44777534237035	39.0	37.0	39.0	35.0	39.0
14	38.715018639572385	40.0	38.0	41.0	35.0	41.0
15	38.810688880677716	40.0	38.0	41.0	35.0	41.0
16	38.849558241167244	40.0	38.0	41.0	35.0	41.0
17	38.8212593657322	40.0	38.0	41.0	35.0	41.0
18	38.65835124421805	40.0	38.0	41.0	35.0	41.0
19	38.46536444378239	40.0	37.0	41.0	35.0	41.0
20	38.295905871610394	40.0	36.0	41.0	34.0	41.0
21	38.218064618473974	40.0	36.0	41.0	34.0	41.0
22	38.235375141949	40.0	36.0	41.0	34.0	41.0
23	38.19519607824171	40.0	36.0	41.0	34.0	41.0
24	38.09257106155605	40.0	36.0	41.0	34.0	41.0
25	38.000303727334106	40.0	36.0	41.0	34.0	41.0
26	38.05469026582913	40.0	36.0	41.0	34.0	41.0
27	38.002712265747874	40.0	36.0	41.0	34.0	41.0
28	37.946996678345016	40.0	36.0	41.0	34.0	41.0
29	37.86836418648471	40.0	36.0	41.0	34.0	41.0
30	37.68785729071349	40.0	35.0	41.0	33.0	41.0
31	37.553563379382524	40.0	35.0	41.0	33.0	41.0
32	37.26649655356531	40.0	35.0	41.0	33.0	41.0
33	36.98549266701618	40.0	35.0	41.0	32.0	41.0
34	36.7547662943911	40.0	35.0	41.0	31.0	41.0
35	36.58517036781961	40.0	35.0	41.0	31.0	41.0
36	36.475302324771576	40.0	35.0	41.0	30.0	41.0
37	36.398120759666554	40.0	35.0	41.0	30.0	41.0
38	36.32762699961889	40.0	35.0	41.0	30.0	41.0
39	36.232001253600714	39.0	35.0	41.0	30.0	41.0
40	36.14031429008089	39.0	35.0	41.0	29.0	41.0
41	36.030192818492935	39.0	35.0	41.0	29.0	41.0
42	35.98415007612529	39.0	35.0	41.0	29.0	41.0
43	35.88871778700782	39.0	35.0	41.0	28.0	41.0
44	35.80331236905386	39.0	35.0	41.0	27.0	41.0
45	35.70897504599438	39.0	35.0	41.0	27.0	41.0
46	35.597377498254055	39.0	35.0	41.0	26.0	41.0
47	35.53357734697075	39.0	35.0	41.0	26.0	41.0
48	35.471820100558894	39.0	35.0	41.0	26.0	41.0
49	35.39488229114877	38.0	35.0	41.0	26.0	41.0
50	35.28753692608592	38.0	35.0	41.0	26.0	41.0
51	34.36053788756672	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	9.0
10	14.0
11	11.0
12	17.0
13	12.0
14	14.0
15	23.0
16	34.0
17	59.0
18	135.0
19	212.0
20	393.0
21	723.0
22	1087.0
23	1765.0
24	3177.0
25	5387.0
26	7906.0
27	8461.0
28	7770.0
29	7002.0
30	7289.0
31	8490.0
32	10355.0
33	13917.0
34	26183.0
35	38654.0
36	34510.0
37	50436.0
38	91799.0
39	190832.0
40	227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.871295058530386	20.75637779037397	24.63248025288686	15.739846898208782
2	36.84154525634006	23.513912453014154	23.96447357475465	15.68006871589113
3	29.878257572386737	22.028743826306656	32.11365206002581	15.979346541280801
4	26.776949997194876	24.39278715291414	31.083107149973593	17.747155699917393
5	23.491858366333858	29.314717620634887	29.507400693736447	17.68602331929481
6	24.75493847103273	32.9043104132046	28.64438945969422	13.696361656068454
7	76.13805858261867	5.970079955737061	13.526699954150715	4.365161507493553
8	77.65901673595647	4.981515193137697	12.949811476250265	4.4096565946555595
9	71.43221947298471	7.7854795119469316	14.913205561498982	5.8690954535693765
10	37.829336191336616	24.54619847517271	22.867572947760834	14.756892385729845
11	29.378171484065923	24.45488681804024	27.635705179421606	18.531236518472234
12	25.80927857987158	22.66405628821983	32.16665925081881	19.36000588108978
13	22.90994000901509	24.172052829210443	34.00856240242518	18.909444759349288
14	20.551893846329445	26.583880010291907	32.892896456063035	19.971329687315613
15	20.168655726034075	25.517545573609386	35.787011690600515	18.52678700975603
16	22.442548136913317	24.12175403502731	33.444055166169804	19.99164266188957
17	22.204402692146232	25.034290235649852	31.141531133986316	21.619775938217604
18	22.90007370707917	25.369937958371946	32.06973734356591	19.660250990982973
19	23.70678898301642	26.735743677344843	29.92488068545649	19.632586654182248
20	23.920171944493347	26.632050778567297	30.574315501121085	18.873461775818274
21	22.96700979472288	27.00252074341618	31.090651969101064	18.939817492759875
22	22.07265854276655	25.176287600766862	30.004971842348105	22.746082014118485
23	21.753648113505033	26.287504038412802	30.5528417851429	21.406006062939266
24	21.555354790283047	25.580805980139715	31.746277405588195	21.117561823989043
25	21.015223123516428	27.52988425473631	30.208681958789814	21.246210662957456
26	19.65212580115339	28.28359233988056	31.44932106300698	20.61496079595907
27	20.99587743344599	27.120529452845847	30.964518069841812	20.919075043866354
28	19.409143933868695	27.876172106997142	31.791739777253724	20.92294418188044
29	21.241374240439846	26.373979273027658	30.746879056549385	21.637767429983114
30	20.958540251610046	27.70090015495898	30.321854245701875	21.018705347729107
31	22.265535072768813	28.756207548301347	28.096132603098017	20.882124775831816
32	22.745695100317075	28.774779410768968	27.506282512850376	20.973242976063577
33	21.603525558558438	28.696235909082997	27.99495464402963	21.70528388832894
34	21.024315597849533	28.941539259176146	28.60182894153926	21.432316201435064
35	21.66736633579088	27.848701227097123	28.119927801884657	22.364004635227342
36	21.432896572137178	30.206553932882063	26.76940517806741	21.591144316913358
37	21.925631298231224	30.0312819808439	27.9936004457247	20.04948627520018
38	21.773187260476178	29.25745437802639	26.731874539330757	22.237483822166677
39	22.648966649964887	27.370669225456606	27.747910181830143	22.232453942748364
40	22.590542665952164	26.933843543666125	28.673794908601284	21.80181888178042
41	20.22766008074891	26.99265444148026	28.271598012036886	24.508087465733947
42	20.777271135650043	27.346487112868562	27.676524585470226	24.19971716601117
43	21.63157680916057	26.650235727233508	28.846745377831002	22.87144208577492
44	21.16302419565457	27.291738809969225	27.911961633627453	23.63327536074876
45	21.81806926143959	27.127106987469794	27.27548843031005	23.77933532078056
46	21.46443004695199	27.841736778671766	27.720052388128714	22.973780786247534
47	20.684992194014058	26.908694146574557	29.515525883566028	22.890787775845357
48	21.0051633646798	26.01821203263231	29.1164242974129	23.86020030527499
49	20.932036656213544	26.3536662984537	29.503144641920947	23.211152403411806
50	20.607609433732303	25.475565426156532	29.90534153848535	24.01148360162581
51	20.52809864754281	25.443258123738904	27.870561856876712	26.158081371841575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	385.0
1	662.0
2	939.0
3	4967.0
4	8995.0
5	6268.5
6	3542.0
7	3547.0
8	3552.0
9	3537.5
10	3523.0
11	3577.5
12	3632.0
13	3568.5
14	3505.0
15	3348.0
16	3191.0
17	3156.0
18	3121.0
19	3006.0
20	2891.0
21	3123.0
22	3355.0
23	3105.0
24	2855.0
25	3327.5
26	4042.5
27	4285.0
28	5307.0
29	6329.0
30	7243.0
31	8157.0
32	9014.0
33	9871.0
34	11192.5
35	12514.0
36	14326.0
37	16138.0
38	17347.5
39	18557.0
40	20877.0
41	23197.0
42	25771.0
43	28345.0
44	31370.5
45	34396.0
46	39712.0
47	45028.0
48	47651.0
49	50274.0
50	47759.0
51	45244.0
52	39125.5
53	33007.0
54	29627.5
55	26248.0
56	24087.5
57	21927.0
58	20854.5
59	19782.0
60	18887.0
61	17992.0
62	16158.5
63	14325.0
64	12362.0
65	10399.0
66	8838.0
67	7277.0
68	6637.0
69	5997.0
70	4930.0
71	3863.0
72	3323.5
73	2784.0
74	2255.0
75	1327.5
76	929.0
77	737.5
78	546.0
79	423.0
80	300.0
81	206.0
82	112.0
83	74.5
84	37.0
85	30.5
86	24.0
87	19.0
88	14.0
89	7.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	516911.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.86627502854865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.81209158676766	29.853998771293792
2	8.937587319475027	6.947414537007707
3	3.2187067328863943	3.7529742334981164
4	1.7142728767555238	2.665096044078459
5	1.1076446202567267	2.1525010222395133
6	0.8548621546968104	1.993518456956636
7	0.6825395576581115	1.8569439116062867
8	0.5635253971555669	1.7521706457136295
9	0.5029521334682033	1.759308835101345
>10	5.506210320952242	41.26313541721863
>50	0.06673689095164734	1.6546212786976138
>100	0.02888611697904571	2.2192598222103994
>500	0.002490182498193596	0.6320002895348412
>1k	9.960729992774381E-4	0.45662746799776127
>5k	4.980364996387191E-4	1.0404292668452593
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5375	1.0398308412860242	No Hit
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	1212	0.23446976365370442	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCC	1147	0.22189506510791993	No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT	906	0.17527195203816517	TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	647	0.12516661475573165	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT	646	0.12497315785502726	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTC	551	0.1065947522881115	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.934569007043766E-4	0.0	0.0	0.18455788327197525	0.0
2	1.934569007043766E-4	0.0	0.0	0.7357165933787441	0.0
3	1.934569007043766E-4	0.0	0.0	1.0669148073846368	0.0
4	1.934569007043766E-4	0.0	0.0	1.4807191179912984	0.0
5	1.934569007043766E-4	0.0	0.0	2.600060745466821	0.0
6	1.934569007043766E-4	0.0	0.0	3.4559140741829832	0.0
7	1.934569007043766E-4	0.0	0.0	4.283329238495602	0.0
8	1.934569007043766E-4	0.0	0.0	5.465157444898638	0.0
9	1.934569007043766E-4	0.0	0.0	6.046688888415995	0.0
10	1.934569007043766E-4	0.0	0.0	7.20414152533028	0.0
11	3.869138014087532E-4	0.0	0.0	8.714072635327938	0.0
12	3.869138014087532E-4	0.0	0.0	9.817744253846406	0.0
13	5.803707021131298E-4	0.0	0.0	10.277204393019302	0.0
14	5.803707021131298E-4	0.0	0.0	10.479753768056783	0.0
15	5.803707021131298E-4	0.0	0.0	10.803020249133796	0.0
16	5.803707021131298E-4	0.0	0.0	11.434270116132177	0.0
17	5.803707021131298E-4	0.0	0.0	12.253366633714508	0.0
18	5.803707021131298E-4	0.0	0.0	13.117732066061663	0.0
19	5.803707021131298E-4	0.0	0.0	13.673727198686041	0.0
20	5.803707021131298E-4	0.0	0.0	14.205540218722373	0.0
21	5.803707021131298E-4	0.0	0.0	14.88399356949262	0.0
22	5.803707021131298E-4	0.0	0.0	15.590885084666413	0.0
23	5.803707021131298E-4	0.0	0.0	16.307062531074013	0.0
24	5.803707021131298E-4	0.0	0.0	16.894591138513206	0.0
25	5.803707021131298E-4	0.0	0.0	17.42176119293263	0.0
26	5.803707021131298E-4	0.0	0.0	17.906757642998503	0.0
27	5.803707021131298E-4	0.0	0.0	18.376664454809436	0.0
28	5.803707021131298E-4	0.0	0.0	18.877911284534473	0.0
29	5.803707021131298E-4	0.0	0.0	19.43197184815181	0.0
30	5.803707021131298E-4	0.0	0.0	19.986032411769145	0.0
31	7.738276028175064E-4	0.0	0.0	20.537771492578027	0.0
32	7.738276028175064E-4	0.0	0.0	21.073066736827037	0.0
33	7.738276028175064E-4	0.0	0.0	21.566381833623197	0.0
34	7.738276028175064E-4	0.0	0.0	22.03299987812215	0.0
35	7.738276028175064E-4	0.0	0.0	22.515094474677458	0.0
36	7.738276028175064E-4	0.0	0.0	22.995834872927833	0.0
37	7.738276028175064E-4	0.0	0.0	23.494760219844423	0.0
38	7.738276028175064E-4	0.0	0.0	24.013418170632857	0.0
39	7.738276028175064E-4	0.0	0.0	24.509828577840285	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	7.0296804E-4	45.0	21
TAGACGT	20	7.0296804E-4	45.0	1
ATTACGG	20	7.0296804E-4	45.0	2
TTGTCGT	25	3.887817E-5	45.0	12
TGTTACG	20	7.0296804E-4	45.0	1
CGGTACA	20	7.0296804E-4	45.0	6
TAAGACG	20	7.0296804E-4	45.0	1
GCGTATG	20	7.0296804E-4	45.0	1
CGTTTTT	3820	0.0	42.70288	1
ATTAGCG	50	1.0786607E-9	40.5	1
CTCACGA	45	1.9248546E-8	40.0	24
TCGGGCA	45	1.9248546E-8	40.0	5
CGACGGT	40	3.4532968E-7	39.375	28
TTCACGG	40	3.4532968E-7	39.375	2
TTAACGG	40	3.4532968E-7	39.375	2
CGGTCTA	40	3.4532968E-7	39.375	31
TAGGGTC	75	0.0	39.0	5
TATCGGG	35	6.2416184E-6	38.57143	3
ATCGCCA	35	6.2416184E-6	38.57143	24
TACACGG	35	6.2416184E-6	38.57143	2
>>END_MODULE