Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550599_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 652050 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2793 | 0.42834138486312395 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC | 2587 | 0.39674871558929525 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG | 2297 | 0.35227359865041025 | No Hit |
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC | 1558 | 0.23893873169235488 | Illumina Single End Adapter 2 (95% over 21bp) |
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC | 1416 | 0.21716126063952151 | No Hit |
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC | 1133 | 0.17375968100605782 | Illumina Single End Adapter 2 (95% over 21bp) |
GAATGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT | 1110 | 0.17023234414538763 | No Hit |
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 907 | 0.1390997622881681 | No Hit |
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT | 784 | 0.1202361782071927 | No Hit |
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT | 780 | 0.1196227283183805 | Illumina Single End Adapter 2 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGAAT | 65 | 0.0 | 45.000004 | 43 |
TAACGCC | 30 | 2.1640517E-6 | 45.000004 | 12 |
ATAACGC | 30 | 2.1640517E-6 | 45.000004 | 11 |
TCGTAAG | 20 | 7.0310384E-4 | 45.0 | 1 |
TACCGTT | 25 | 3.8889422E-5 | 45.0 | 14 |
GGTCGAA | 70 | 0.0 | 41.785713 | 42 |
TATACGG | 45 | 1.9261279E-8 | 40.0 | 2 |
TGCGTAG | 45 | 1.9261279E-8 | 40.0 | 1 |
CGATGAA | 485 | 0.0 | 39.896908 | 19 |
TGCGCGT | 40 | 3.4551522E-7 | 39.375 | 37 |
TGTAACG | 40 | 3.4551522E-7 | 39.375 | 1 |
CTTGCGT | 35 | 6.2441304E-6 | 38.571426 | 15 |
CCGATGA | 485 | 0.0 | 38.041237 | 18 |
GGCGATA | 245 | 0.0 | 37.65306 | 8 |
GTCGATG | 30 | 1.1393802E-4 | 37.500004 | 1 |
CGTAAGG | 120 | 0.0 | 37.500004 | 2 |
TATAGCG | 30 | 1.1393802E-4 | 37.500004 | 1 |
ACCGTTC | 30 | 1.1393802E-4 | 37.500004 | 15 |
GCGATAT | 90 | 0.0 | 37.5 | 9 |
CACGACG | 55 | 2.744855E-9 | 36.81818 | 26 |