FastQCFastQC Report
Sat 18 Jun 2016
SRR3550599_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550599_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652050
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27930.42834138486312395No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC25870.39674871558929525No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG22970.35227359865041025No Hit
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC15580.23893873169235488Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC14160.21716126063952151No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC11330.17375968100605782Illumina Single End Adapter 2 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT11100.17023234414538763No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9070.1390997622881681No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT7840.1202361782071927No Hit
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT7800.1196227283183805Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAT650.045.00000443
TAACGCC302.1640517E-645.00000412
ATAACGC302.1640517E-645.00000411
TCGTAAG207.0310384E-445.01
TACCGTT253.8889422E-545.014
GGTCGAA700.041.78571342
TATACGG451.9261279E-840.02
TGCGTAG451.9261279E-840.01
CGATGAA4850.039.89690819
TGCGCGT403.4551522E-739.37537
TGTAACG403.4551522E-739.3751
CTTGCGT356.2441304E-638.57142615
CCGATGA4850.038.04123718
GGCGATA2450.037.653068
GTCGATG301.1393802E-437.5000041
CGTAAGG1200.037.5000042
TATAGCG301.1393802E-437.5000041
ACCGTTC301.1393802E-437.50000415
GCGATAT900.037.59
CACGACG552.744855E-936.8181826