##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550599_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	652050
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.333532704547196	33.0	31.0	34.0	31.0	34.0
2	32.45999386550111	34.0	31.0	34.0	31.0	34.0
3	32.42113794954375	34.0	31.0	34.0	30.0	34.0
4	35.971062035120006	37.0	35.0	37.0	35.0	37.0
5	36.04935050993022	37.0	35.0	37.0	35.0	37.0
6	36.096446591519054	37.0	35.0	37.0	35.0	37.0
7	36.14581397132122	37.0	37.0	37.0	35.0	37.0
8	36.15340388007055	37.0	37.0	37.0	35.0	37.0
9	37.95890192469903	39.0	38.0	39.0	35.0	39.0
10	37.6829307568438	39.0	38.0	39.0	35.0	39.0
11	37.580463154666056	39.0	37.0	39.0	35.0	39.0
12	37.473244383099455	39.0	37.0	39.0	35.0	39.0
13	37.45935741124147	39.0	37.0	39.0	35.0	39.0
14	38.76787823019707	40.0	38.0	41.0	35.0	41.0
15	38.85783298826777	40.0	38.0	41.0	35.0	41.0
16	38.924421440073615	40.0	38.0	41.0	35.0	41.0
17	38.85178437236408	40.0	38.0	41.0	35.0	41.0
18	38.77116478797638	40.0	38.0	41.0	35.0	41.0
19	38.689595889885744	40.0	38.0	41.0	35.0	41.0
20	38.542532014416075	40.0	38.0	41.0	35.0	41.0
21	38.493583314163025	40.0	38.0	41.0	35.0	41.0
22	38.49252818035427	40.0	37.0	41.0	35.0	41.0
23	38.44350586611456	40.0	37.0	41.0	35.0	41.0
24	38.42052296603021	40.0	37.0	41.0	34.0	41.0
25	38.355092400889504	40.0	37.0	41.0	34.0	41.0
26	38.33667356797792	40.0	37.0	41.0	34.0	41.0
27	38.25180124223603	40.0	37.0	41.0	34.0	41.0
28	38.177412775093934	40.0	37.0	41.0	34.0	41.0
29	38.12809293765815	40.0	37.0	41.0	34.0	41.0
30	38.02409631163254	40.0	37.0	41.0	34.0	41.0
31	37.989465531784376	40.0	37.0	41.0	34.0	41.0
32	37.80255961966107	40.0	36.0	41.0	34.0	41.0
33	37.694951307415074	40.0	36.0	41.0	33.0	41.0
34	37.58477417375968	40.0	36.0	41.0	33.0	41.0
35	37.526395215090865	40.0	36.0	41.0	33.0	41.0
36	37.4257848324515	40.0	36.0	41.0	33.0	41.0
37	37.34031746031746	40.0	36.0	41.0	33.0	41.0
38	37.27037650486926	40.0	35.0	41.0	33.0	41.0
39	37.2012882447665	40.0	35.0	41.0	33.0	41.0
40	37.113985123840195	40.0	35.0	41.0	32.0	41.0
41	37.017241009125065	40.0	35.0	41.0	32.0	41.0
42	36.972758224062574	40.0	35.0	41.0	32.0	41.0
43	36.88876619891113	40.0	35.0	41.0	32.0	41.0
44	36.838484778774635	39.0	35.0	41.0	32.0	41.0
45	36.787895100069015	39.0	35.0	41.0	31.0	41.0
46	36.715368453339465	39.0	35.0	41.0	31.0	41.0
47	36.62287861360325	39.0	35.0	41.0	31.0	41.0
48	36.55333640058278	39.0	35.0	41.0	31.0	41.0
49	36.47548807606778	39.0	35.0	41.0	31.0	41.0
50	36.36852695345449	39.0	35.0	41.0	31.0	41.0
51	35.44827390537535	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	15.0
10	16.0
11	20.0
12	17.0
13	16.0
14	18.0
15	19.0
16	55.0
17	88.0
18	172.0
19	344.0
20	687.0
21	1082.0
22	1688.0
23	2284.0
24	2932.0
25	3801.0
26	4941.0
27	5586.0
28	5933.0
29	6210.0
30	7324.0
31	9063.0
32	11577.0
33	16393.0
34	32819.0
35	48073.0
36	39768.0
37	60099.0
38	114179.0
39	276477.0
40	338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.9901081205429	20.157963346369144	24.56989494670654	12.282033586381411
2	31.31723027375201	26.420366536308563	25.519055287171227	16.743347902768193
3	31.10282953761215	26.014876159803695	27.12614063338701	15.756153669197149
4	28.950387240242314	23.93773483628556	29.36124530327429	17.750632620197837
5	27.186412084962807	29.731462311172457	25.10589678705621	17.976228816808526
6	26.505329345909058	35.54926769419523	23.367073077218002	14.57832988267771
7	79.40556705774097	6.152135572425427	10.24875392991335	4.193543439920251
8	81.28502415458937	7.1416302430795175	7.948316846867572	3.625028755463538
9	75.13902308105207	8.315773330266083	9.506326201978375	7.038877386703474
10	40.83168468675715	29.640365002683843	16.255501878690286	13.272448431868721
11	31.389003910743043	25.2259796027912	25.505712752089565	17.879303734376197
12	28.753776550878	23.000230043708306	28.48493213710605	19.761061268307646
13	26.281573498964804	23.456790123456788	29.893719806763286	20.367916570815122
14	20.41806609922552	29.32903918411165	28.715435932827237	21.537458783835596
15	20.36040180967717	26.516524806379877	33.05804769572885	20.065025688214096
16	22.97247143623955	25.377195000383406	30.851008358254735	20.799325205122308
17	22.69488536155203	26.528947166628324	28.21148684916801	22.564680622651636
18	23.41461544360095	26.70101986044015	28.25718886588452	21.62717583007438
19	25.54359328272372	26.804846254121617	26.68599033816425	20.965570124990414
20	28.007361398665747	26.227129821332717	27.7489456330036	18.016563146997928
21	26.33279656468062	26.758070700099683	27.1910129591289	19.718119776090788
22	24.645809370447054	25.200214707461082	28.057510927076144	22.09646499501572
23	24.704700559773023	25.993098688750866	28.493980522966027	20.808220228510084
24	23.809217084579405	25.833141630243077	28.182194616977224	22.17544666820029
25	23.745878383559543	27.486849168008586	26.979066022544284	21.788206425887584
26	22.21808143547274	27.97147458017023	27.971627942642435	21.838816041714594
27	24.210720036806993	27.42366382946093	27.803849398052293	20.56176673567978
28	21.651100375738057	28.536922015182885	29.27766275592363	20.534314853155433
29	23.61352657004831	26.58937198067633	29.34759604324822	20.449505406027146
30	23.71474580170232	26.434935971167857	29.002223755846945	20.848094471282877
31	24.854075607698796	26.677095314776473	26.73015873015873	21.738670347366
32	26.212253661529022	27.275669043784983	27.337014032666207	19.175063262019783
33	25.24714362395522	27.047925772563453	26.79809830534468	20.906832298136646
34	23.225366152902385	27.844950540602714	27.964573268921093	20.965110037573805
35	24.93121693121693	26.12268997776244	27.56721110344299	21.37888198757764
36	24.5778697952611	28.63123993558776	27.080591979142703	19.710298290008435
37	24.755616900544435	28.85622268230964	27.109117398972472	19.279043018173454
38	23.959358944866192	27.219998466375277	26.528333716739517	22.292308872019017
39	24.918334483551874	26.441990644889195	26.887815351583466	21.75185951997546
40	23.95123073383943	26.440610382639367	28.771566597653553	20.836592285867646
41	22.216547810750708	27.541906295529483	27.758454106280194	22.483091787439612
42	23.780231577333026	27.658768499348206	27.281036730312092	21.279963193006672
43	25.049306034813277	26.22728318380492	27.15282570355034	21.570585077831456
44	23.683766582317308	26.53615520282187	28.050456253354806	21.72962196150602
45	22.815428264703627	25.978989341308186	27.37949543746645	23.82608695652174
46	23.09194080208573	28.168238632006748	27.343301894026535	21.39651867188099
47	23.59144237405107	26.90713902308105	29.304807913503566	20.196610689364313
48	23.427651253738212	26.04125450502262	28.012115635304042	22.51897860593513
49	23.056053983590218	26.131891726094626	28.890269151138714	21.921785139176446
50	21.409861206962656	26.182194616977227	29.072310405643737	23.33563377041638
51	21.84172992868645	25.965186718809907	28.113948316846866	24.079135035656773
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	486.0
1	1067.5
2	1649.0
3	3448.5
4	5248.0
5	3605.0
6	1962.0
7	1924.0
8	1886.0
9	1832.5
10	1779.0
11	1895.0
12	2011.0
13	1930.5
14	1850.0
15	1847.5
16	1845.0
17	1839.5
18	1834.0
19	1816.5
20	1799.0
21	1919.5
22	2040.0
23	2410.0
24	2780.0
25	3635.5
26	5020.5
27	5550.0
28	7537.0
29	9524.0
30	11196.0
31	12868.0
32	14122.0
33	15376.0
34	16581.0
35	17786.0
36	19843.5
37	21901.0
38	23583.5
39	25266.0
40	27344.5
41	29423.0
42	32778.0
43	36133.0
44	39004.0
45	41875.0
46	48120.0
47	54365.0
48	53649.5
49	52934.0
50	52328.0
51	51722.0
52	46585.0
53	41448.0
54	38947.0
55	36446.0
56	35410.5
57	34375.0
58	33405.5
59	32436.0
60	30837.5
61	29239.0
62	27134.0
63	25029.0
64	21107.0
65	17185.0
66	13948.0
67	10711.0
68	8845.0
69	6979.0
70	5820.0
71	4661.0
72	3905.5
73	3150.0
74	2433.5
75	1376.5
76	1036.0
77	863.5
78	691.0
79	525.5
80	360.0
81	226.5
82	93.0
83	88.0
84	83.0
85	53.5
86	24.0
87	13.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	652050.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.68100092616769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.54797566432761	18.152376554890285
2	7.430229428888081	3.6677099883205035
3	2.6398347709520604	1.9546129328039248
4	1.6053842134340166	1.5848995703447981
5	1.0765681880321782	1.3285390222952433
6	0.8976843557543172	1.329344904946713
7	0.7544947009441204	1.3035179088953317
8	0.6540136109461824	1.2913368429991203
9	0.6367892973767927	1.4144937514497267
>10	10.415112180041755	58.63410977162786
>50	0.2839938009510827	4.245976922669882
>100	0.04857788700479046	2.281849452160677
>500	0.004982347385106714	0.8292743546249145
>1k	0.004359553961968374	1.9819580219710187
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2793	0.42834138486312395	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC	2587	0.39674871558929525	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG	2297	0.35227359865041025	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1558	0.23893873169235488	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	1416	0.21716126063952151	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1133	0.17375968100605782	Illumina Single End Adapter 2 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	1110	0.17023234414538763	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	907	0.1390997622881681	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	784	0.1202361782071927	No Hit
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	780	0.1196227283183805	Illumina Single End Adapter 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.067249444061038E-4	0.0	0.0	0.12253661529023847	0.0
2	3.067249444061038E-4	0.0	0.0	0.6577716432788897	0.0
3	3.067249444061038E-4	0.0	0.0	0.8548424200598114	0.0
4	3.067249444061038E-4	0.0	0.0	1.146077754773407	0.0
5	3.067249444061038E-4	0.0	0.0	2.106586918181121	0.0
6	3.067249444061038E-4	0.0	0.0	2.602407790813588	0.0
7	3.067249444061038E-4	0.0	0.0	2.9589755386856837	0.0
8	3.067249444061038E-4	0.0	0.0	3.418296142933824	0.0
9	3.067249444061038E-4	0.0	0.0	3.595736523272755	0.0
10	3.067249444061038E-4	0.0	0.0	4.341384863123993	0.0
11	3.067249444061038E-4	0.0	0.0	4.955141476880607	0.0
12	3.067249444061038E-4	0.0	0.0	5.616747181964573	0.0
13	3.067249444061038E-4	0.0	0.0	5.8291542059658	0.0
14	3.067249444061038E-4	0.0	0.0	5.932980599647267	0.0
15	3.067249444061038E-4	0.0	0.0	6.1234567901234565	0.0
16	3.067249444061038E-4	0.0	0.0	6.422360248447205	0.0
17	3.067249444061038E-4	0.0	0.0	6.763898474043401	0.0
18	3.067249444061038E-4	0.0	0.0	7.109884211333487	0.0
19	3.067249444061038E-4	0.0	0.0	7.450655624568668	0.0
20	3.067249444061038E-4	0.0	0.0	7.696649029982363	0.0
21	3.067249444061038E-4	0.0	0.0	7.981443140863431	0.0
22	3.067249444061038E-4	0.0	0.0	8.307338394294916	0.0
23	4.6008741660915573E-4	0.0	0.0	8.600260716202746	0.0
24	4.6008741660915573E-4	0.0	0.0	8.8341384863124	0.0
25	4.6008741660915573E-4	0.0	0.0	9.051606471896328	0.0
26	4.6008741660915573E-4	0.0	0.0	9.27582240625719	0.0
27	4.6008741660915573E-4	0.0	0.0	9.487769342841807	0.0
28	6.134498888122076E-4	0.0	0.0	9.692661605705084	0.0
29	7.668123610152595E-4	0.0	0.0	9.910129591289012	0.0
30	7.668123610152595E-4	0.0	0.0	10.152902384786442	0.0
31	9.201748332183115E-4	0.0	0.0	10.393221378728626	0.0
32	9.201748332183115E-4	0.0	0.0	10.615290238478645	0.0
33	9.201748332183115E-4	0.0	0.0	10.84472049689441	0.0
34	9.201748332183115E-4	0.0	0.0	11.071083505866115	0.0
35	9.201748332183115E-4	0.0	0.0	11.306801625642205	0.0
36	9.201748332183115E-4	0.0	0.0	11.538225596196611	0.0
37	9.201748332183115E-4	0.0	0.0	11.779924852388621	0.0
38	9.201748332183115E-4	0.0	0.0	12.010275285637604	0.0
39	9.201748332183115E-4	0.0	0.0	12.267464151522123	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAT	65	0.0	45.000004	43
TAACGCC	30	2.1640517E-6	45.000004	12
ATAACGC	30	2.1640517E-6	45.000004	11
TCGTAAG	20	7.0310384E-4	45.0	1
TACCGTT	25	3.8889422E-5	45.0	14
GGTCGAA	70	0.0	41.785713	42
TATACGG	45	1.9261279E-8	40.0	2
TGCGTAG	45	1.9261279E-8	40.0	1
CGATGAA	485	0.0	39.896908	19
TGCGCGT	40	3.4551522E-7	39.375	37
TGTAACG	40	3.4551522E-7	39.375	1
CTTGCGT	35	6.2441304E-6	38.571426	15
CCGATGA	485	0.0	38.041237	18
GGCGATA	245	0.0	37.65306	8
GTCGATG	30	1.1393802E-4	37.500004	1
CGTAAGG	120	0.0	37.500004	2
TATAGCG	30	1.1393802E-4	37.500004	1
ACCGTTC	30	1.1393802E-4	37.500004	15
GCGATAT	90	0.0	37.5	9
CACGACG	55	2.744855E-9	36.81818	26
>>END_MODULE