FastQCFastQC Report
Sat 18 Jun 2016
SRR3550597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882587
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCG188912.140412219985112No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC142011.6090198473351636No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGC113941.2909775466894482No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC57020.6460552897334767No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46170.5231212333741603No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT41950.47530725016343994No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC33060.37458063624322585No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT29630.3357176119747968No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTA26530.30059359587213497No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT26300.297987620483873No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCCCTT25630.290396300874588No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTC21690.24575480944088232No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCC14590.16530948223801167No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC10620.12032808097105442No Hit
ACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC10450.11840192524929553No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTT9090.10299267947522453No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8890.1007266139202141No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAT207.0323946E-445.00000436
GCGAACT207.0323946E-445.00000426
ATTACGG207.0323946E-445.0000042
TGGCGAA207.0323946E-445.00000424
CGACAAT253.8900664E-545.00000420
TCTAGCG253.8900664E-545.0000041
ATCCGTC207.0323946E-445.00000427
TCCGTCA207.0323946E-445.00000428
ACGTCGT406.8121153E-945.00000422
CCACGTC253.8900664E-545.00000433
TACACCG207.0323946E-445.00000425
TCGCGCG253.8900664E-545.0000041
CGAATAT1100.042.95454414
ACGGGCG950.042.631585
CGCATCG750.042.021
GCGATTC556.002665E-1140.909099
TACGGCT14100.040.8510677
CTCGAAT1050.040.71428743
TGCGTAG501.0804797E-940.5000041
CGATGAA12250.040.4081619