##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550596_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	743117
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12069566434357	33.0	31.0	34.0	30.0	34.0
2	32.29391468638182	34.0	31.0	34.0	30.0	34.0
3	32.140767873699566	33.0	31.0	34.0	30.0	34.0
4	35.813326838169495	37.0	35.0	37.0	35.0	37.0
5	35.95586293948328	37.0	35.0	37.0	35.0	37.0
6	36.00299279924965	37.0	35.0	37.0	35.0	37.0
7	36.12399662502675	37.0	36.0	37.0	35.0	37.0
8	36.12579042062017	37.0	37.0	37.0	35.0	37.0
9	37.953727340378435	39.0	38.0	39.0	35.0	39.0
10	37.60392643419542	39.0	37.0	39.0	35.0	39.0
11	37.52741762064385	39.0	37.0	39.0	35.0	39.0
12	37.484121612074546	39.0	37.0	39.0	35.0	39.0
13	37.509468899244666	39.0	37.0	39.0	35.0	39.0
14	38.76642305316659	40.0	38.0	41.0	35.0	41.0
15	38.85975694271561	40.0	38.0	41.0	35.0	41.0
16	38.89487523498991	40.0	38.0	41.0	35.0	41.0
17	38.83754240583919	40.0	38.0	41.0	35.0	41.0
18	38.62668597273377	40.0	38.0	41.0	35.0	41.0
19	38.413131444981076	40.0	37.0	41.0	35.0	41.0
20	38.20465417962448	40.0	36.0	41.0	34.0	41.0
21	38.12180854428038	40.0	35.0	41.0	34.0	41.0
22	38.14123348005765	40.0	35.0	41.0	34.0	41.0
23	38.105132839108784	40.0	35.0	41.0	34.0	41.0
24	38.005187608411596	40.0	35.0	41.0	34.0	41.0
25	37.932723918306266	40.0	35.0	41.0	34.0	41.0
26	37.965788698145786	40.0	35.0	41.0	34.0	41.0
27	37.91617336166445	40.0	35.0	41.0	34.0	41.0
28	37.85541442330077	40.0	35.0	41.0	34.0	41.0
29	37.760130638916884	40.0	35.0	41.0	34.0	41.0
30	37.56995062688648	40.0	35.0	41.0	33.0	41.0
31	37.41258644331916	40.0	35.0	41.0	33.0	41.0
32	37.07448356046221	40.0	35.0	41.0	33.0	41.0
33	36.738905179130604	40.0	35.0	41.0	31.0	41.0
34	36.436573244859154	40.0	35.0	41.0	30.0	41.0
35	36.239043111649984	40.0	35.0	41.0	29.0	41.0
36	36.11522680816076	40.0	35.0	41.0	29.0	41.0
37	36.048250813801864	40.0	35.0	41.0	28.0	41.0
38	35.954731219982854	40.0	35.0	41.0	27.0	41.0
39	35.86133139196116	39.0	35.0	41.0	26.0	41.0
40	35.767967897383585	39.0	35.0	41.0	25.0	41.0
41	35.652707447144934	39.0	35.0	41.0	25.0	41.0
42	35.61427204599006	39.0	35.0	41.0	24.0	41.0
43	35.52100544059684	39.0	35.0	41.0	24.0	41.0
44	35.43700924618869	39.0	35.0	41.0	23.0	41.0
45	35.33815805586469	39.0	35.0	41.0	23.0	41.0
46	35.211182088419456	39.0	35.0	41.0	23.0	41.0
47	35.16692526210543	39.0	35.0	41.0	23.0	41.0
48	35.08059430749128	39.0	35.0	41.0	23.0	41.0
49	35.01468274847702	38.0	35.0	41.0	23.0	41.0
50	34.93302535132422	38.0	35.0	41.0	23.0	41.0
51	34.074126954436515	37.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	18.0
10	23.0
11	23.0
12	20.0
13	23.0
14	15.0
15	38.0
16	56.0
17	123.0
18	230.0
19	437.0
20	794.0
21	1148.0
22	1809.0
23	2992.0
24	5132.0
25	9091.0
26	12851.0
27	14120.0
28	12912.0
29	11531.0
30	11354.0
31	12970.0
32	15268.0
33	20255.0
34	36770.0
35	52798.0
36	50054.0
37	68657.0
38	126851.0
39	274476.0
40	268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.648087717008224	20.483046411264983	24.45927088197417	19.409594989752623
2	39.57815525684381	23.090442016533064	21.857527145792655	15.473875580830477
3	28.310481391221032	22.44545609910687	34.162318988799875	15.081743520872218
4	25.305436425219717	24.329547029606374	33.6646853725591	16.70033117261481
5	23.605838649903042	27.319520344710185	32.253871193903514	16.820769811483252
6	23.911712422135412	31.060923111703808	31.731207871707955	13.296156594452825
7	73.10100562899248	5.988155297214302	16.673282941986255	4.23755613180697
8	74.39582192306192	5.210754161188615	16.224497622850777	4.168926292898695
9	68.97473749086618	7.635271431012883	17.780511009706412	5.60948006841453
10	34.91213362095067	28.597381031519937	24.031747356069097	12.458737991460294
11	25.70025985140967	24.73890383344749	32.47335211009841	17.08748420504443
12	23.52415568477104	22.184528142943844	36.01855427880132	18.2727618934838
13	22.000304124384183	22.76424842925138	36.87467787710415	18.36076956926029
14	19.11354470426595	26.03560408387912	36.23480555551817	18.616045656336755
15	18.342871983819506	25.995771863650003	37.949878686667105	17.711477465863386
16	20.83776848060265	26.031836171154744	35.929470056532146	17.20092529171046
17	20.55975034886835	25.823928129756148	34.33322074451264	19.283100776862863
18	21.472392638036812	25.754894585913117	34.50963980100038	18.263072975049692
19	21.126148372328988	27.96450626213638	32.9335757357186	17.975769629816032
20	22.3741348939669	27.949300042927288	32.86319650875972	16.813368554346088
21	21.24739442106694	27.347106848585078	34.180754847487	17.224743882860977
22	20.78892018349735	25.20289537179206	33.649613721661595	20.358570723048995
23	19.548334918996606	26.73805067035204	34.03757416396072	19.67604024669063
24	20.383600429003778	25.5845311034467	34.309133016738954	19.72273545081057
25	19.971552258930963	27.211058285572797	32.62608714374722	20.191302311749023
26	18.80794006865675	28.389742126744512	33.13637018127697	19.665947623321763
27	19.52909165043997	27.354911810657	33.153056651913495	19.96293988698953
28	18.190809791728626	27.880939340642186	33.41963647716308	20.508614390466104
29	19.442833362714083	27.170149518851	32.80869634256786	20.578320775867056
30	20.33044594592776	26.71651974049847	32.101405296877886	20.85162901669589
31	21.273096968579644	27.747447575550016	29.877260243003455	21.102195212866885
32	20.89711310601157	28.353004977681845	29.823298350057932	20.926583566248652
33	21.12170761804669	28.13325492486378	29.059219476879143	21.685817980210384
34	19.019616022779722	28.757517322305908	30.512422673683954	21.710443981230412
35	18.641613635537876	29.709857263391903	28.834355828220858	22.814173272849363
36	19.26170441532087	31.235323643517777	28.280876362672362	21.222095578488986
37	20.281597648822462	29.35109814470669	29.07590594751567	21.291398258955184
38	19.705914411862466	30.054755845983877	28.374401339223837	21.86492840292982
39	19.98500909008945	28.250060219319433	29.26564726685031	22.49928342374081
40	20.139358943477273	27.563627261925106	30.057178075592404	22.239835719005217
41	18.099572476474098	29.185444553145736	30.30397635903902	22.411006611341147
42	19.806706077239518	28.57531182842002	30.135900537869542	21.48208155647092
43	20.371085576026385	27.20594468973257	30.325641857204182	22.09732787703686
44	20.067095760156207	27.545325971549566	29.132155501758135	23.25542276653609
45	21.201641195128087	26.79954838874632	28.223684830248803	23.775125585876786
46	20.655697555028347	28.423518772952306	28.685523275608016	22.235260396411334
47	19.28215879868177	28.233104612059744	29.845232984846266	22.639503604412226
48	19.347155293177252	27.093041876312885	30.547545003007603	23.012257827502264
49	20.90047731380119	25.861472688688323	30.693013347830828	22.54503664967966
50	18.68965452277367	26.09965860019351	30.69812694367105	24.51255993336177
51	18.561814626768058	25.791362598352613	29.91480480193563	25.732017972943694
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	979.0
1	1379.5
2	1780.0
3	12412.5
4	23045.0
5	14740.0
6	6435.0
7	6583.0
8	6731.0
9	6967.5
10	7204.0
11	7328.0
12	7452.0
13	7424.0
14	7396.0
15	7116.5
16	6837.0
17	6597.5
18	6358.0
19	5965.0
20	5572.0
21	5580.5
22	5589.0
23	5669.5
24	5750.0
25	6204.5
26	7257.0
27	7855.0
28	8771.5
29	9688.0
30	11048.5
31	12409.0
32	13965.5
33	15522.0
34	17310.0
35	19098.0
36	20459.0
37	21820.0
38	24698.5
39	27577.0
40	29858.0
41	32139.0
42	36103.5
43	40068.0
44	44111.5
45	48155.0
46	56004.5
47	63854.0
48	66946.0
49	70038.0
50	67444.0
51	64850.0
52	57263.0
53	49676.0
54	43974.0
55	38272.0
56	34266.0
57	30260.0
58	27546.0
59	24832.0
60	23634.5
61	22437.0
62	19756.5
63	17076.0
64	13998.5
65	10921.0
66	8857.5
67	6794.0
68	5569.0
69	4344.0
70	3619.5
71	2895.0
72	2413.5
73	1932.0
74	1675.5
75	1040.5
76	662.0
77	494.5
78	327.0
79	284.5
80	242.0
81	167.5
82	93.0
83	64.0
84	35.0
85	35.0
86	35.0
87	19.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	743117.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.39330972775075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4365536187702	28.578656581517702
2	9.837654228032864	7.554002115427649
3	3.766281428589212	4.338000282291035
4	2.0368539692792185	3.1280626125094213
5	1.385308886723636	2.6593296578293066
6	1.056387914023985	2.433493702546523
7	0.8504871069516439	2.285711044165719
8	0.7007862120717889	2.1524401674407527
9	0.6213715647587607	2.1470859847620205
>10	5.195910453125352	36.56473279359286
>50	0.0785775251983985	2.0014034366864784
>100	0.02783687818249228	1.769525127564075
>500	0.003523655466138163	1.0540038431843626
>1k	0.002114193279682898	1.2163455980067521
>5k	0.0	0.0
>10k+	3.523655466138163E-4	2.1172070524753273
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15650	2.105994076302924	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCC	2414	0.3248479041658312	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT	1544	0.20777347308701052	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTC	1393	0.18745365803769798	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTT	1344	0.18085981077004024	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGT	1193	0.1605399957207277	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCG	1103	0.14842884767809106	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTC	964	0.12972385236779674	No Hit
CCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	964	0.12972385236779674	No Hit
GCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	946	0.1273016227592694	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGC	826	0.11115342536908723	No Hit
CTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCT	802	0.10792378589105081	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0370493475455415E-4	0.0	0.0	0.11196083523859635	0.0
2	4.0370493475455415E-4	0.0	0.0	0.5209139341449597	0.0
3	4.0370493475455415E-4	0.0	0.0	0.845627269999206	0.0
4	4.0370493475455415E-4	0.0	0.0	1.3023521195181915	0.0
5	4.0370493475455415E-4	0.0	0.0	2.4856112832837898	0.0
6	4.0370493475455415E-4	0.0	0.0	3.469574777592223	0.0
7	4.0370493475455415E-4	0.0	0.0	4.412494936867277	0.0
8	4.0370493475455415E-4	0.0	0.0	5.623206036196184	0.0
9	4.0370493475455415E-4	0.0	0.0	6.312599496445378	0.0
10	5.382732463394055E-4	0.0	0.0	7.425344864940514	0.0
11	5.382732463394055E-4	0.0	0.0	9.010559575410063	0.0
12	5.382732463394055E-4	0.0	0.0	10.144028463889267	0.0
13	6.728415579242569E-4	0.0	0.0	10.62066942352281	0.0
14	6.728415579242569E-4	0.0	0.0	10.836651563616497	0.0
15	6.728415579242569E-4	0.0	0.0	11.12328206729223	0.0
16	6.728415579242569E-4	0.0	0.0	11.685373904782153	0.0
17	6.728415579242569E-4	0.0	0.0	12.406794623188542	0.0
18	6.728415579242569E-4	0.0	0.0	13.18755996700385	0.0
19	6.728415579242569E-4	0.0	0.0	13.724622098538992	0.0
20	6.728415579242569E-4	0.0	0.0	14.223735966207206	0.0
21	6.728415579242569E-4	0.0	0.0	14.80776243848546	0.0
22	6.728415579242569E-4	0.0	0.0	15.461226159541498	0.0
23	6.728415579242569E-4	0.0	0.0	16.11455531228595	0.0
24	6.728415579242569E-4	0.0	0.0	16.610574108787713	0.0
25	6.728415579242569E-4	0.0	0.0	17.05088162429335	0.0
26	6.728415579242569E-4	0.0	0.0	17.48082737980695	0.0
27	6.728415579242569E-4	0.0	0.0	17.907274359219343	0.0
28	6.728415579242569E-4	0.0	0.0	18.372207875745005	0.0
29	9.419781810939596E-4	0.0	0.0	18.857864912254733	0.0
30	9.419781810939596E-4	0.0	0.0	19.397214705086817	0.0
31	0.001076546492678811	0.0	0.0	19.886774222632507	0.0
32	0.001076546492678811	0.0	0.0	20.360454679411184	0.0
33	0.001076546492678811	0.0	0.0	20.836826502421555	0.0
34	0.001076546492678811	0.0	0.0	21.283458728571677	0.0
35	0.0012111148042636623	0.0	0.0	21.745970015488812	0.0
36	0.0012111148042636623	0.0	0.0	22.22180356525285	0.0
37	0.0012111148042636623	0.0	0.0	22.70032848124858	0.0
38	0.0012111148042636623	0.0	0.0	23.20630533280762	0.0
39	0.0012111148042636623	0.0	0.0	23.757497137059172	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	30	2.164461E-6	45.000004	21
CGGTCTA	30	2.164461E-6	45.000004	31
ATAACGC	35	1.2111559E-7	45.000004	11
TCGATTG	20	7.0316764E-4	45.0	1
TCTAGCG	20	7.0316764E-4	45.0	1
TCGCTAG	20	7.0316764E-4	45.0	1
CGGTACC	25	3.88947E-5	45.0	21
CCACGAA	20	7.0316764E-4	45.0	10
TGCGTAG	45	3.8380676E-10	45.0	1
GTACGAG	25	3.88947E-5	45.0	1
CGTTTTT	7605	0.0	43.66864	1
CGGGTAC	85	0.0	42.35294	6
ACGGGTC	65	0.0	41.53846	5
AGCGACT	170	0.0	41.02941	19
ACGCGAG	40	3.4560253E-7	39.375	1
CGCGAGG	80	0.0	39.375	2
TAACGCC	40	3.4560253E-7	39.375	12
ACGTAGG	75	0.0	39.0	2
AACGAGC	180	0.0	38.75	15
TCACGAC	35	6.2453073E-6	38.57143	25
>>END_MODULE