##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550595_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	575747
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37082607464737	33.0	31.0	34.0	31.0	34.0
2	32.50351803830502	34.0	31.0	34.0	31.0	34.0
3	32.46970283822582	34.0	31.0	34.0	31.0	34.0
4	36.00958233390708	37.0	35.0	37.0	35.0	37.0
5	36.09901223975114	37.0	35.0	37.0	35.0	37.0
6	36.1328986516647	37.0	36.0	37.0	35.0	37.0
7	36.21845532846893	37.0	37.0	37.0	35.0	37.0
8	36.22313099330088	37.0	37.0	37.0	35.0	37.0
9	38.05160252680431	39.0	39.0	39.0	37.0	39.0
10	37.7044708873863	39.0	38.0	39.0	35.0	39.0
11	37.58334824150191	39.0	37.0	39.0	35.0	39.0
12	37.421431635770574	39.0	37.0	39.0	35.0	39.0
13	37.381912541446155	39.0	37.0	39.0	35.0	39.0
14	38.61226372000201	40.0	38.0	41.0	35.0	41.0
15	38.71849788188214	40.0	38.0	41.0	35.0	41.0
16	38.772629297243405	40.0	38.0	41.0	35.0	41.0
17	38.70611223332471	40.0	38.0	41.0	35.0	41.0
18	38.63258861965412	40.0	38.0	41.0	35.0	41.0
19	38.56362430025688	40.0	37.0	41.0	35.0	41.0
20	38.48995652604356	40.0	37.0	41.0	35.0	41.0
21	38.399274334039085	40.0	37.0	41.0	34.0	41.0
22	38.400854889387176	40.0	37.0	41.0	35.0	41.0
23	38.3666332607899	40.0	37.0	41.0	34.0	41.0
24	38.29031154309097	40.0	36.0	41.0	34.0	41.0
25	38.215191742206216	40.0	36.0	41.0	34.0	41.0
26	38.22834682594959	40.0	36.0	41.0	34.0	41.0
27	38.17436999237513	40.0	36.0	41.0	34.0	41.0
28	38.11271270193331	40.0	36.0	41.0	34.0	41.0
29	38.06278625854759	40.0	36.0	41.0	34.0	41.0
30	37.9310426280988	40.0	36.0	41.0	34.0	41.0
31	37.889305545665025	40.0	36.0	41.0	34.0	41.0
32	37.732665563172716	40.0	35.0	41.0	34.0	41.0
33	37.62461810482729	40.0	35.0	41.0	33.0	41.0
34	37.52766753452471	40.0	35.0	41.0	33.0	41.0
35	37.451201656283054	40.0	35.0	41.0	33.0	41.0
36	37.402845347001374	40.0	35.0	41.0	33.0	41.0
37	37.356408283499526	40.0	35.0	41.0	33.0	41.0
38	37.28441311895676	40.0	35.0	41.0	33.0	41.0
39	37.19696151260884	40.0	35.0	41.0	33.0	41.0
40	37.08720670711267	40.0	35.0	41.0	33.0	41.0
41	37.000027789984145	40.0	35.0	41.0	33.0	41.0
42	36.97423521095203	39.0	35.0	41.0	33.0	41.0
43	36.88588216699349	39.0	35.0	41.0	32.0	41.0
44	36.786685818597405	39.0	35.0	41.0	32.0	41.0
45	36.6858845986171	39.0	35.0	41.0	32.0	41.0
46	36.593517638824	39.0	35.0	41.0	32.0	41.0
47	36.514507240159304	39.0	35.0	41.0	31.0	41.0
48	36.45327374697567	39.0	35.0	41.0	31.0	41.0
49	36.36314909152805	39.0	35.0	41.0	31.0	41.0
50	36.273236334709516	38.0	35.0	41.0	31.0	41.0
51	35.41521710056674	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	8.0
9	20.0
10	15.0
11	22.0
12	15.0
13	17.0
14	24.0
15	23.0
16	52.0
17	79.0
18	160.0
19	243.0
20	407.0
21	634.0
22	944.0
23	1467.0
24	2171.0
25	3244.0
26	4361.0
27	4983.0
28	5328.0
29	5869.0
30	6639.0
31	8069.0
32	10380.0
33	14898.0
34	34747.0
35	50199.0
36	33862.0
37	51667.0
38	100062.0
39	234855.0
40	280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.35488851874174	21.099371772670985	24.900867915942246	11.644871792645032
2	32.43177993111558	24.513718699359266	25.3052121852133	17.74928918431186
3	32.49760745605275	23.997693431316186	26.77964453136534	16.72505458126573
4	29.031675371300242	26.22592909732921	26.522066115846023	18.220329415524528
5	26.169480692040082	30.46807017665746	24.659963490908332	18.70248564039413
6	27.1838151132355	34.44377478302101	23.908939169461586	14.463470934281897
7	81.71036931152051	6.061342916246199	7.904860989288698	4.323426782944592
8	83.93999447674065	5.370588122908152	7.241722492692102	3.4476949076590935
9	78.38147658606992	7.618624152622592	8.745508009594491	5.254391251712992
10	43.40482885711953	28.265366558575206	15.345108181197645	12.984696403107614
11	33.67781334509776	25.3420339142019	22.894778435667053	18.085374305033287
12	31.349012673969646	23.607070466715417	26.053804883047587	18.990111976267354
13	26.63583136342873	24.33621017565007	27.292022363989737	21.735936096931464
14	21.699461742744646	27.182772988830163	29.17722541324575	21.940539855179445
15	21.443272826432445	26.67317415462	32.101947556826175	19.781605462121384
16	25.892275600220238	25.15063039842153	30.3534364920703	18.60365750928793
17	24.989274802995066	25.35888159208819	27.717730183570215	21.93411342134653
18	26.01924109027055	26.385200443944996	26.772697035329756	20.822861430454694
19	26.98824309983378	27.633665481539634	25.421061681606677	19.95702973701991
20	29.146135368486505	26.967574299127918	25.90860221590386	17.97768811648172
21	27.317033349717846	26.831750751632228	26.95732674247543	18.893889156174502
22	26.544645477961677	24.346457732302557	27.66822927431667	21.440667515419097
23	25.597701768311428	26.153501451158235	27.547863905500158	20.70093287503018
24	25.12648784969787	24.802560847038716	28.64643671612705	21.424514587136365
25	24.45674923186747	26.446164721657254	26.71954869065753	22.377537355817747
26	23.41462482652971	28.042699310634706	26.646078920081216	21.89659694275437
27	23.238332114626736	28.289335419897977	27.572006454223818	20.90032601125147
28	21.594901927409087	29.567848377846516	27.59701743995192	21.240232254792467
29	23.750883634652027	27.655376406650838	27.71147743713819	20.882262521558946
30	24.194133881722355	25.95376094013516	28.330846708710595	21.521258469431885
31	25.872127861717036	26.786070965198256	26.00986197062251	21.33193920246219
32	26.50365525135172	27.779736585687807	26.13300633785326	19.58360182510721
33	25.35193409605261	27.61803361545957	25.560185289719268	21.469846998768556
34	22.763470760594497	28.809876560364188	27.482904817567437	20.943747861473877
35	22.52100314895258	28.647826215334167	26.82532431779931	22.005846317913942
36	23.277585467227794	31.584359102175085	24.75966005901898	20.378395371578144
37	23.96191382673292	30.097421263158992	25.05805501374736	20.882609896360727
38	24.113890302511347	28.78938144705921	25.066044634188277	22.030683616241163
39	24.81558740210544	26.844777306698948	25.53135318117159	22.80828211002402
40	24.32075199697089	25.990235294321984	27.238179269713953	22.450833438993172
41	21.140014624479157	27.530147790609416	27.263190255442062	24.06664732946937
42	23.96747182356139	27.574959140038942	25.20065236987774	23.256916666521928
43	24.169122895994246	26.633399739816273	25.951503003923598	23.24597436026588
44	23.048839160256154	27.280385308130136	25.864832990879677	23.805942540734037
45	23.305549138771024	26.58702520377874	25.948376630707585	24.15904902674265
46	22.8086294848258	27.703835191499042	26.28237750261834	23.20515782105682
47	23.06586052554334	27.57044326761581	27.085508044331974	22.27818816250888
48	22.968595581045147	26.43904353822078	27.625675861098713	22.96668501963536
49	23.19056807938209	26.99362740926136	27.254505885397577	22.561298625958973
50	21.15564649055922	26.930405195337535	27.54230590867169	24.37164240543155
51	20.558682893701572	26.607346629682834	27.22880015006591	25.605170326549683
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	489.0
1	1109.5
2	1730.0
3	2982.5
4	4235.0
5	2998.5
6	1762.0
7	1746.5
8	1731.0
9	1726.5
10	1722.0
11	1701.0
12	1680.0
13	1796.5
14	1913.0
15	1737.0
16	1561.0
17	1661.5
18	1762.0
19	1651.5
20	1541.0
21	1680.0
22	1819.0
23	2166.5
24	2514.0
25	2946.0
26	4384.0
27	5390.0
28	6363.0
29	7336.0
30	9055.5
31	10775.0
32	11405.5
33	12036.0
34	12961.5
35	13887.0
36	15236.5
37	16586.0
38	18714.0
39	20842.0
40	23428.0
41	26014.0
42	26978.5
43	27943.0
44	31034.0
45	34125.0
46	36707.0
47	39289.0
48	40855.0
49	42421.0
50	42856.5
51	43292.0
52	41132.5
53	38973.0
54	37342.5
55	35712.0
56	34482.5
57	33253.0
58	33235.5
59	33218.0
60	32516.5
61	31815.0
62	28721.5
63	25628.0
64	21921.0
65	18214.0
66	14514.5
67	10815.0
68	9202.0
69	7589.0
70	6276.0
71	4963.0
72	4148.5
73	3334.0
74	2609.0
75	1563.5
76	1243.0
77	939.0
78	635.0
79	459.5
80	284.0
81	290.5
82	297.0
83	164.5
84	32.0
85	32.0
86	32.0
87	33.5
88	35.0
89	24.0
90	13.0
91	7.5
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	575747.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.195367083589108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.57816481114735	18.538288717536762
2	7.239055037131904	3.6478129799768624
3	2.63878270463467	1.994552966912898
4	1.533059862254627	1.5450402396248752
5	1.0899643487049968	1.373102593682376
6	0.911700419958976	1.378237605067725
7	0.8020831911912746	1.4146146303548524
8	0.7722265131572006	1.5565224376540578
9	0.693759983758109	1.5731583712821338
>10	10.395452202274228	59.97720614619874
>50	0.29872879987967293	4.5326748001148305
>100	0.04563912220383892	2.031654398990584
>500	6.91501851573317E-4	0.09896061217962882
>1k	6.91501851573317E-4	0.33817350042369637
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1941	0.3371272451267658	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.947496035585075E-4	0.0	0.0	0.058358966698914626	0.0
2	6.947496035585075E-4	0.0	0.0	0.2973528303230412	0.0
3	6.947496035585075E-4	0.0	0.0	0.4093812038968505	0.0
4	6.947496035585075E-4	0.0	0.0	0.536694068748947	0.0
5	6.947496035585075E-4	0.0	0.0	0.925406471939932	0.0
6	8.684370044481344E-4	0.0	0.0	1.1835059496619176	0.0
7	8.684370044481344E-4	0.0	0.0	1.4273630605109535	0.0
8	8.684370044481344E-4	0.0	0.0	1.7769957985017726	0.0
9	8.684370044481344E-4	0.0	0.0	1.9329670845006575	0.0
10	8.684370044481344E-4	0.0	0.0	2.2895473185270614	0.0
11	8.684370044481344E-4	0.0	0.0	2.8491681241934392	0.0
12	8.684370044481344E-4	0.0	0.0	3.2936341830699942	0.0
13	8.684370044481344E-4	0.0	0.0	3.4746164547969856	0.0
14	8.684370044481344E-4	0.0	0.0	3.557812719823117	0.0
15	8.684370044481344E-4	0.0	0.0	3.712741881416664	0.0
16	8.684370044481344E-4	0.0	0.0	3.995678657465866	0.0
17	8.684370044481344E-4	0.0	0.0	4.338016524619321	0.0
18	8.684370044481344E-4	0.0	0.0	4.693033572037718	0.0
19	8.684370044481344E-4	0.0	0.0	4.946964552138352	0.0
20	8.684370044481344E-4	0.0	0.0	5.193426974000733	0.0
21	8.684370044481344E-4	0.0	0.0	5.476711124851715	0.0
22	8.684370044481344E-4	0.0	0.0	5.776495578787211	0.0
23	8.684370044481344E-4	0.0	0.0	6.0747168461147	0.0
24	8.684370044481344E-4	0.0	0.0	6.31214752313082	0.0
25	8.684370044481344E-4	0.0	0.0	6.53099364825175	0.0
26	8.684370044481344E-4	0.0	0.0	6.733513157689054	0.0
27	8.684370044481344E-4	0.0	0.0	6.942285413558386	0.0
28	8.684370044481344E-4	0.0	0.0	7.1658210985033355	0.0
29	8.684370044481344E-4	0.0	0.0	7.393351593668747	0.0
30	8.684370044481344E-4	0.0	0.0	7.648672072976498	0.0
31	0.001215811806227388	0.0	0.0	7.885929062591729	0.0
32	0.001389499207117015	0.0	0.0	8.107206811325113	0.0
33	0.001389499207117015	0.0	0.0	8.342900614332336	0.0
34	0.001389499207117015	0.0	0.0	8.573731170114652	0.0
35	0.001389499207117015	0.0	0.0	8.82523052660283	0.0
36	0.001389499207117015	0.0	0.0	9.078466757099907	0.0
37	0.001389499207117015	0.0	0.0	9.338476796231678	0.0
38	0.001389499207117015	0.0	0.0	9.619155636069316	0.0
39	0.001389499207117015	0.0	0.0	9.94725113634982	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	20	7.03035E-4	45.0	2
CGCATCG	55	1.8189894E-12	45.0	21
TTATACG	20	7.03035E-4	45.0	1
AATTGCG	25	3.888372E-5	45.0	1
TTGCGTA	20	7.03035E-4	45.0	45
CGGTCTA	55	1.8189894E-12	45.0	31
CGGTAGA	20	7.03035E-4	45.0	24
CGATACG	20	7.03035E-4	45.0	10
CGTATGG	30	2.1636079E-6	44.999996	2
CGACGGT	60	3.6379788E-12	41.249996	28
CACGACG	60	3.6379788E-12	41.249996	26
TAACGCC	60	3.6379788E-12	41.249996	12
ATAACGC	60	3.6379788E-12	41.249996	11
TACACGG	60	3.6379788E-12	41.249996	2
ACGGTCT	60	3.6379788E-12	41.249996	30
TCGTTAG	55	6.002665E-11	40.909092	1
TCGTACG	55	6.002665E-11	40.909092	30
ATAGCGG	140	0.0	40.178574	2
TCGTAAG	45	1.9254003E-8	40.000004	1
TATAGCG	40	3.4542063E-7	39.375	1
>>END_MODULE