##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550590_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1391443
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.201512386781204	33.0	31.0	34.0	30.0	34.0
2	32.34008076507626	34.0	31.0	34.0	30.0	34.0
3	32.284555673498666	34.0	31.0	34.0	30.0	34.0
4	35.886086602182054	37.0	35.0	37.0	35.0	37.0
5	35.978870855651294	37.0	35.0	37.0	35.0	37.0
6	36.016928469222236	37.0	35.0	37.0	35.0	37.0
7	36.119603893224514	37.0	36.0	37.0	35.0	37.0
8	36.07778256098166	37.0	37.0	37.0	35.0	37.0
9	37.89855423470455	39.0	38.0	39.0	35.0	39.0
10	37.57688816573873	39.0	37.0	39.0	35.0	39.0
11	37.49597216702373	39.0	37.0	39.0	35.0	39.0
12	37.483106386679154	39.0	37.0	39.0	35.0	39.0
13	37.485373816965556	39.0	37.0	39.0	35.0	39.0
14	38.7968281848412	40.0	38.0	41.0	35.0	41.0
15	38.86384135031043	40.0	38.0	41.0	35.0	41.0
16	38.934226554734906	40.0	38.0	41.0	35.0	41.0
17	38.8475589729511	40.0	38.0	41.0	35.0	41.0
18	38.727375824952944	40.0	38.0	41.0	35.0	41.0
19	38.64280965874995	40.0	38.0	41.0	35.0	41.0
20	38.452704853881905	40.0	37.0	41.0	34.0	41.0
21	38.46163227670842	40.0	37.0	41.0	34.0	41.0
22	38.45503696522243	40.0	37.0	41.0	34.0	41.0
23	38.3854559619043	40.0	37.0	41.0	34.0	41.0
24	38.33244552597555	40.0	37.0	41.0	34.0	41.0
25	38.324089452460505	40.0	37.0	41.0	34.0	41.0
26	38.31412282069765	40.0	37.0	41.0	34.0	41.0
27	38.24561121080777	40.0	37.0	41.0	34.0	41.0
28	38.210820709148706	40.0	37.0	41.0	34.0	41.0
29	38.142681374659254	40.0	37.0	41.0	34.0	41.0
30	38.05842280280256	40.0	37.0	41.0	34.0	41.0
31	37.97706769159786	40.0	37.0	41.0	34.0	41.0
32	37.75853197004836	40.0	37.0	41.0	33.0	41.0
33	37.586283448190116	40.0	37.0	41.0	33.0	41.0
34	37.452538839176306	40.0	37.0	41.0	33.0	41.0
35	37.34702822896806	40.0	37.0	41.0	33.0	41.0
36	37.27936537824402	40.0	36.0	41.0	32.0	41.0
37	37.22011681398376	40.0	36.0	41.0	32.0	41.0
38	37.163295226610074	40.0	36.0	41.0	32.0	41.0
39	37.06387325963047	40.0	36.0	41.0	32.0	41.0
40	37.01998860176091	40.0	36.0	41.0	31.0	41.0
41	36.93138490042352	40.0	35.0	41.0	31.0	41.0
42	36.887480838237714	40.0	35.0	41.0	31.0	41.0
43	36.80498159105332	40.0	35.0	41.0	31.0	41.0
44	36.808874671833486	40.0	35.0	41.0	31.0	41.0
45	36.76322206515107	40.0	35.0	41.0	31.0	41.0
46	36.66817684950084	40.0	35.0	41.0	31.0	41.0
47	36.56757553130096	39.0	35.0	41.0	31.0	41.0
48	36.552082981480375	39.0	35.0	41.0	31.0	41.0
49	36.52579875711761	39.0	35.0	41.0	31.0	41.0
50	36.43074635468359	39.0	35.0	41.0	30.0	41.0
51	35.602753400606424	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	17.0
9	27.0
10	51.0
11	43.0
12	37.0
13	34.0
14	35.0
15	55.0
16	62.0
17	139.0
18	292.0
19	604.0
20	1185.0
21	2262.0
22	3916.0
23	5940.0
24	7933.0
25	9980.0
26	13392.0
27	14983.0
28	15097.0
29	15655.0
30	16844.0
31	20432.0
32	25620.0
33	34289.0
34	62281.0
35	89642.0
36	80073.0
37	118282.0
38	236057.0
39	615258.0
40	925.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.27003405816839	20.72603764581086	24.41005488546782	13.593873410552929
2	32.31307355026401	27.57051492587192	24.158158113555498	15.95825341030858
3	29.672361713702966	26.58053545851321	28.413812136034316	15.333290691749502
4	27.16769569432596	23.88211374810179	31.91758483818597	17.032605719386275
5	26.748778067085755	28.855655603571257	26.469571516763533	17.92599481257946
6	24.777802612108438	35.61676619164421	25.592065215750843	14.013365980496506
7	77.20373741504322	5.477119795780352	13.172727880337176	4.146414908839241
8	78.70304424974648	7.538936197889528	10.063006533505146	3.6950130188588393
9	72.43933096792323	8.013192060328738	11.460835981064262	8.086640990683772
10	35.610513689745105	33.88525437261893	18.382355583376395	12.121876354259571
11	25.920644970724638	24.649734124933612	31.292262780437284	18.137358123904466
12	24.728573143132703	22.036978877323758	33.459581168614164	19.774866810929375
13	23.362078072907046	23.260385082249147	34.55362526528216	18.82391157956165
14	18.45954164130331	28.71896297584594	32.12715145356296	20.694343929287797
15	18.228702145901774	27.10725484263459	34.963918751971875	19.700124259491766
16	20.78058533479273	26.762936031156144	31.865121316503803	20.591357317547324
17	20.73926132798828	27.505402664715696	30.94126026003221	20.81407574726381
18	20.34715040429252	26.95755413624561	31.77348982315481	20.921805636307056
19	21.274892324011834	28.000643935827767	29.921311904260538	20.803151835899854
20	23.951178740343657	27.26831066741505	31.67194056817275	17.10857002406854
21	22.663450820479174	28.171976861430903	31.061063945846147	18.10350837224378
22	20.77124251586303	24.83623116433803	31.991105636378926	22.401420683420017
23	21.33914217111301	26.37585585611484	32.10681285543138	20.178189117340775
24	21.084370685683854	26.76811051548644	31.716570495521555	20.43094830330815
25	19.840410279113122	30.110180582316342	29.428011064772324	20.621398073798208
26	18.90806881776688	28.572927529190917	30.978128460885568	21.54087519215663
27	21.00991560559793	27.832545062931075	30.33368955824996	20.82384977322104
28	18.044217405959138	29.421614827197377	31.728572424454327	20.80559534238916
29	19.779322616880464	27.055078792304105	31.607331381881977	21.55826720893346
30	19.37779700641708	28.203239370926443	30.540453327948036	21.87851029470844
31	20.89715496790023	27.94976150657986	28.494591585857272	22.658491939662635
32	21.81447605112103	28.002584367451632	29.786919047348686	20.39602053407865
33	20.808254452392227	26.762792295480303	28.750944163720682	23.678009088406785
34	19.295148992808187	28.03341566991964	29.17410199339822	23.49733334387395
35	20.848069234600338	26.59138750203925	29.072912077605768	23.487631185754644
36	19.78823422878264	29.940213145633702	28.805635588378397	21.465917037205262
37	19.320374603918378	28.46275413365837	30.673696299453155	21.543174962970095
38	19.536337456870314	28.025366472072516	29.04739899514389	23.390897075913276
39	20.740698684746697	25.855676445244253	30.03953449764022	23.36409037236883
40	21.276185945094408	25.478442163998093	30.329808695002235	22.915563195905257
41	19.219975234343053	27.18142245136883	29.88818083097906	23.710421483309055
42	20.42059933464756	27.23072378818248	30.613039844248018	21.735637032921936
43	20.941928630924874	26.19208979455141	29.719722618892764	23.14625895563095
44	19.820502888009067	26.737063609504663	29.566428520607747	23.876004981878523
45	20.119185622407816	25.949967048596314	28.708470271509505	25.222377057486366
46	21.400661040373194	27.623697125933294	28.65248522576922	22.32315660792429
47	19.03383753412824	26.535618059812727	31.850532145405886	22.58001226065315
48	19.487395459246265	26.340065672830292	30.484683885721513	23.68785498220193
49	19.50996196035339	24.948129387980682	32.28274532266144	23.259163329004494
50	19.00688709490795	25.17415373824152	31.415444254633503	24.403514912217027
51	19.14005819857515	24.435567967929696	30.029688603845074	26.394685229650083
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	833.0
1	1998.0
2	3163.0
3	11715.5
4	20268.0
5	13388.5
6	6509.0
7	6267.0
8	6025.0
9	5918.0
10	5811.0
11	5714.5
12	5618.0
13	5598.0
14	5578.0
15	5315.5
16	5053.0
17	5087.5
18	5122.0
19	5160.0
20	5198.0
21	5696.5
22	6195.0
23	7091.0
24	7987.0
25	10363.0
26	15270.0
27	17801.0
28	21290.5
29	24780.0
30	29389.5
31	33999.0
32	37327.0
33	40655.0
34	45000.5
35	49346.0
36	52440.0
37	55534.0
38	59279.5
39	63025.0
40	68522.5
41	74020.0
42	78605.5
43	83191.0
44	89045.0
45	94899.0
46	121092.5
47	147286.0
48	133328.0
49	119370.0
50	112448.5
51	105527.0
52	93678.0
53	81829.0
54	75281.5
55	68734.0
56	61707.0
57	54680.0
58	51684.5
59	48689.0
60	45858.5
61	43028.0
62	37775.5
63	32523.0
64	26939.0
65	21355.0
66	17427.5
67	13500.0
68	11082.0
69	8664.0
70	7030.0
71	5396.0
72	4452.5
73	3509.0
74	2736.5
75	1536.0
76	1108.0
77	782.5
78	457.0
79	380.5
80	304.0
81	215.5
82	127.0
83	76.0
84	25.0
85	17.0
86	9.0
87	6.5
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1391443.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.3500325375672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.58346771752416	19.1257673604622
2	10.084129754969977	5.314342943130168
3	3.5981611356153813	2.8443498899662507
4	1.7244829867489708	1.8176073124526575
5	1.1045504701868543	1.4552470414404375
6	0.8117697221731773	1.2834095155365064
7	0.6196894799262218	1.1430186571470828
8	0.5291557356119141	1.1154616680651392
9	0.45253809192910927	1.0731954102138543
>10	8.005455820147196	49.7087034767896
>50	0.42344301813328505	6.733099861307841
>100	0.05272158640977295	2.5674439259087873
>500	0.005491831907409035	1.007543023053129
>1k	0.003844282335186324	1.8491037340858718
>5k	5.491831907409035E-4	1.202105550090103
>10k+	5.491831907409035E-4	1.7596006303503802
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC	12294	0.883543199398035	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12025	0.8642107509973459	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG	9929	0.7135757627153969	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC	6685	0.4804364965003956	No Hit
GCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	4129	0.29674230277488906	No Hit
GAACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT	3240	0.23285179486331817	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCT	2276	0.1635711991076889	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCC	1956	0.14057349097304023	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1940	0.13942360556630778	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTC	1713	0.12310960635829135	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACATCCGTTCGTA	1486	0.10679560715027493	No Hit
CTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGCT	1457	0.1047114398505724	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.437356758415544E-4	0.0	0.0	0.1081610960707697	0.0
2	1.437356758415544E-4	0.0	0.0	0.6260407361278902	0.0
3	1.437356758415544E-4	0.0	0.0	0.8394882147525986	0.0
4	1.437356758415544E-4	0.0	0.0	1.3277583055863589	0.0
5	1.437356758415544E-4	0.0	0.0	2.9517558390821614	0.0
6	1.437356758415544E-4	0.0	0.0	3.5820367776473776	0.0
7	1.437356758415544E-4	0.0	0.0	4.254288533558327	0.0
8	1.437356758415544E-4	0.0	0.0	5.0644546704392495	0.0
9	1.437356758415544E-4	0.0	0.0	5.383835342159183	0.0
10	1.437356758415544E-4	0.0	0.0	6.678965649329509	0.0
11	1.437356758415544E-4	0.0	0.0	7.633226801241589	0.0
12	2.1560351376233162E-4	0.0	0.0	8.819908540989463	0.0
13	2.1560351376233162E-4	0.0	0.0	9.138426798654347	0.0
14	2.1560351376233162E-4	0.0	0.0	9.288414976395009	0.0
15	2.1560351376233162E-4	0.0	0.0	9.613473207310683	0.0
16	2.1560351376233162E-4	0.0	0.0	10.06717486810455	0.0
17	2.1560351376233162E-4	0.0	0.0	10.640680214712352	0.0
18	2.1560351376233162E-4	0.0	0.0	11.238620626213219	0.0
19	2.1560351376233162E-4	0.0	0.0	11.742486037875787	0.0
20	2.1560351376233162E-4	0.0	0.0	12.154001277810158	0.0
21	2.1560351376233162E-4	0.0	0.0	12.641337086750948	0.0
22	2.1560351376233162E-4	0.0	0.0	13.224328987964293	0.0
23	2.1560351376233162E-4	0.0	0.0	13.732793941253792	0.0
24	2.1560351376233162E-4	0.0	0.0	14.151999039845686	0.0
25	2.874713516831088E-4	0.0	0.0	14.525855532709569	0.0
26	2.874713516831088E-4	0.0	0.0	14.866940291481576	0.0
27	3.5933918960388605E-4	0.0	0.0	15.224698388651206	0.0
28	4.3120702752466323E-4	0.0	0.0	15.59223051177806	0.0
29	5.030748654454404E-4	0.0	0.0	15.979957497360653	0.0
30	5.030748654454404E-4	0.0	0.0	16.423885132197295	0.0
31	5.030748654454404E-4	0.0	0.0	16.822679764819686	0.0
32	5.030748654454404E-4	0.0	0.0	17.22614580690693	0.0
33	5.030748654454404E-4	0.0	0.0	17.61078247545893	0.0
34	5.030748654454404E-4	0.0	0.0	17.999731214286175	0.0
35	5.030748654454404E-4	0.0	0.0	18.424973211263413	0.0
36	5.749427033662176E-4	0.0	0.0	18.82772057497145	0.0
37	5.749427033662176E-4	0.0	0.0	19.24484150626364	0.0
38	5.749427033662176E-4	0.0	0.0	19.648020076999202	0.0
39	6.468105412869949E-4	0.0	0.0	20.0731183383006	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGT	20	7.0337986E-4	45.000004	14
CGTAAGG	160	0.0	45.000004	2
CGCCGAT	20	7.0337986E-4	45.000004	31
ATCGCGC	20	7.0337986E-4	45.000004	44
CGGTCTA	130	0.0	43.26923	31
CGTTTTT	5980	0.0	42.328594	1
CGACGGT	135	0.0	41.666664	28
TAGCCGT	60	3.6379788E-12	41.250004	44
CGATCGA	140	0.0	40.17857	41
CGATGAA	1340	0.0	39.96269	19
TGCGTAG	115	0.0	39.130436	1
TACGGCT	840	0.0	38.839287	7
CTCCGAT	35	6.249238E-6	38.571426	38
CTAAACG	35	6.249238E-6	38.571426	1
CTCGAAT	100	0.0	38.250004	43
CCGATGA	1345	0.0	38.141266	18
CGCATCG	65	9.094947E-12	38.07692	21
GCCGCAT	65	9.094947E-12	38.07692	19
ATTACGG	95	0.0	37.894737	2
GCGAGAC	60	1.5643309E-10	37.500004	21
>>END_MODULE