FastQCFastQC Report
Sat 18 Jun 2016
SRR3550588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837655
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC58050.6930060705182922RNA PCR Primer, Index 35 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC41500.4954306964084259RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT34580.4128191200434546TruSeq Adapter, Index 10 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31140.37175209364237066No Hit
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC18600.22204845670353546RNA PCR Primer, Index 35 (95% over 23bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG14890.1777581462535292No Hit
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC14270.170356531030078TruSeq Adapter, Index 10 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCC13340.15925410819490124No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA12590.15030054139233934No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12550.14982301782953603No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11100.13251278867791633No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA9910.1183064626845181No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9710.11591884487050158No Hit
GCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG9550.11400875061928838RNA PCR Primer, Index 35 (95% over 22bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC9220.11006918122616113No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8590.10254818511200912No Hit
ACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG8470.1011156144235992TruSeq Adapter, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATA207.0321886E-445.00000445
ATTACCG207.0321886E-445.00000443
TGTTGCG302.1647938E-645.0000041
GTCAACG207.0321886E-445.0000041
ACTCGAA207.0321886E-445.00000439
ATAACGG406.8121153E-945.0000042
TAATGCG302.1647938E-645.0000041
ACGAAAT207.0321886E-445.00000444
CGCGATG253.889897E-545.029
TCTAGCG453.8380676E-1045.01
ATAACGC351.2113924E-745.011
AACGGGC750.042.04
TTTACGG603.6379788E-1241.2500042
TAAACGG603.6379788E-1241.2500042
TAATCGT556.002665E-1140.90909221
CGTTTTT18900.040.3571431
CCCTCGT1400.040.1785714
GGCCTAT900.040.08
GTTCGCG403.4567165E-739.3750041
GCGTATG403.4567165E-739.3750041
Produced by FastQC (version 0.11.5)