##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550588_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	837655
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07755460183488	33.0	31.0	34.0	30.0	34.0
2	32.19359163378718	33.0	31.0	34.0	30.0	34.0
3	32.16839271537805	33.0	31.0	34.0	30.0	34.0
4	35.78763213972339	37.0	35.0	37.0	35.0	37.0
5	35.919006034704026	37.0	35.0	37.0	35.0	37.0
6	35.97333627806197	37.0	35.0	37.0	35.0	37.0
7	36.045768245876886	37.0	35.0	37.0	35.0	37.0
8	36.01464087243555	37.0	35.0	37.0	35.0	37.0
9	37.81162889256317	39.0	38.0	39.0	35.0	39.0
10	37.552418358393375	39.0	37.0	39.0	35.0	39.0
11	37.50136989572079	39.0	37.0	39.0	35.0	39.0
12	37.391623042899525	39.0	37.0	39.0	35.0	39.0
13	37.3643301836675	39.0	37.0	39.0	35.0	39.0
14	38.537258179083274	40.0	38.0	41.0	35.0	41.0
15	38.63869970333849	40.0	38.0	41.0	35.0	41.0
16	38.67177417910715	40.0	38.0	41.0	35.0	41.0
17	38.63660576251559	40.0	38.0	41.0	35.0	41.0
18	38.56069145411894	40.0	38.0	41.0	35.0	41.0
19	38.51138834006841	40.0	37.0	41.0	34.0	41.0
20	38.43450107741254	40.0	37.0	41.0	34.0	41.0
21	38.34470157761847	40.0	37.0	41.0	34.0	41.0
22	38.34458816577231	40.0	37.0	41.0	34.0	41.0
23	38.317864753388925	40.0	37.0	41.0	34.0	41.0
24	38.2265395658117	40.0	37.0	41.0	34.0	41.0
25	38.11456864699667	40.0	36.0	41.0	34.0	41.0
26	38.14753090472808	40.0	37.0	41.0	34.0	41.0
27	38.1187636914959	40.0	37.0	41.0	34.0	41.0
28	38.04814750702855	40.0	36.0	41.0	34.0	41.0
29	38.016697805182325	40.0	36.0	41.0	34.0	41.0
30	37.93198751275883	40.0	36.0	41.0	34.0	41.0
31	37.84697757429968	40.0	36.0	41.0	34.0	41.0
32	37.7381821871773	40.0	36.0	41.0	34.0	41.0
33	37.64714590135557	40.0	36.0	41.0	33.0	41.0
34	37.57116951489575	40.0	36.0	41.0	33.0	41.0
35	37.563513618375104	40.0	36.0	41.0	33.0	41.0
36	37.524716022706244	40.0	36.0	41.0	33.0	41.0
37	37.485371662557974	40.0	36.0	41.0	33.0	41.0
38	37.425429323528185	40.0	35.0	41.0	33.0	41.0
39	37.359014152604594	40.0	35.0	41.0	33.0	41.0
40	37.27591908363228	40.0	35.0	41.0	33.0	41.0
41	37.200562283995204	40.0	35.0	41.0	33.0	41.0
42	37.19683640639643	40.0	35.0	41.0	33.0	41.0
43	37.111192555407655	39.0	35.0	41.0	32.0	41.0
44	37.022270505160236	39.0	35.0	41.0	32.0	41.0
45	36.96024019435209	39.0	35.0	41.0	32.0	41.0
46	36.870740340593684	39.0	35.0	41.0	32.0	41.0
47	36.82786469369848	39.0	35.0	41.0	32.0	41.0
48	36.80593681169455	39.0	35.0	41.0	32.0	41.0
49	36.76597167091464	39.0	35.0	41.0	32.0	41.0
50	36.68192155481672	39.0	35.0	41.0	31.0	41.0
51	35.70850051632235	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	26.0
10	33.0
11	23.0
12	20.0
13	21.0
14	13.0
15	25.0
16	59.0
17	84.0
18	145.0
19	260.0
20	405.0
21	695.0
22	1049.0
23	1739.0
24	2678.0
25	4054.0
26	5676.0
27	6986.0
28	7752.0
29	8741.0
30	10511.0
31	13160.0
32	16944.0
33	23319.0
34	47293.0
35	67684.0
36	53730.0
37	80644.0
38	154031.0
39	329452.0
40	392.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.72480317075645	21.348287779575124	22.691919704412914	12.234989345255505
2	31.666975067300978	24.42807599787502	25.58034035491939	18.324608579904613
3	31.970083148790373	23.87665566372791	27.39110970506951	16.76215148241221
4	29.788994275686292	25.84715664563573	25.755233359796097	18.60861571888188
5	24.05608514245125	30.1375864765327	25.405924873605485	20.40040350741057
6	25.47815031247948	34.312694367012675	25.343369286878247	14.865786033629597
7	75.10371214879635	7.632856008738681	11.30441530224257	5.9590165402224065
8	73.34463472431968	7.9985196769553095	11.465101981125882	7.191743617599131
9	67.04944159588375	10.151554040744697	14.619383875223093	8.179620488148462
10	38.244742764025766	25.954241304594373	21.13674484125326	14.664271090126604
11	29.234708800162355	24.857130919053787	25.441261617252923	20.466898663530927
12	27.443637296977876	22.581850523186752	29.775026711474297	20.19948546836108
13	24.07220156269586	25.62666014051131	30.376587019715757	19.92455127707708
14	18.994454757626947	28.891488739397488	30.044230620004658	22.06982588297091
15	19.550650327402092	27.601458834484365	33.573368510902455	19.274522327211084
16	21.35771886994049	26.933880893685348	30.768633864777268	20.9397663715969
17	20.74075842679862	27.636795578131807	27.92008643176487	23.7023595633047
18	21.303997469125115	27.621992347684905	30.50026562248181	20.573744560708167
19	23.15416251320651	27.89728468164101	27.188042809987405	21.760509995165073
20	23.999379219368354	28.1707863022366	28.254472306617878	19.575362171777165
21	23.80908607959124	27.99923596229951	28.830604485140064	19.36107347296918
22	20.89464039491199	26.627788289928432	27.826491813455423	24.651079501704164
23	22.021834764909183	26.41612597071587	29.143501799666925	22.418537464708024
24	23.62118055762814	25.087058514543575	28.650219959291118	22.641540968537164
25	20.53625896102811	27.432773635924097	27.194608758975953	24.836358644071844
26	18.96198315535632	27.25537363234267	28.602945126573587	25.17969808572742
27	21.22019208385314	26.901409291414723	28.97803988515558	22.900358739576557
28	17.88146671362315	27.05469435507458	29.72930383033588	25.334535100966388
29	20.101831899767806	24.80830413475715	29.665554434701637	25.42430953077341
30	20.162954915806626	25.375602127367475	31.227295246849835	23.234147709976064
31	20.93475237418746	26.07553228954641	27.1864908584083	25.803224477857828
32	20.720344294488783	25.74448907963302	29.52874393395849	24.006422691919703
33	19.92455127707708	25.421683151177994	29.85978714387188	24.79397842787305
34	17.730211125105207	24.853788254114164	29.829583778524572	27.58641684225606
35	18.322937247434805	23.76825781497156	30.93708030155613	26.971724636037507
36	19.355701332887644	25.53557252090658	29.085363305895623	26.02336284031015
37	19.003050181757406	25.842142648226297	30.738191737648556	24.416615432367742
38	18.30550763739248	27.13241131492082	29.53781688165175	25.024264166034943
39	20.8823441631698	23.147596564217963	30.615110039336003	25.35494923327623
40	21.000173102291516	22.679981615342832	31.974500241746302	24.34534504061935
41	17.849233873133926	24.749329974750943	29.263121452149154	28.138314699965978
42	19.112641839420764	23.690063331562516	29.603118228865107	27.594176600151616
43	21.7927428356543	22.35156478502486	29.91016587974763	25.945526499573212
44	19.729363520781227	23.767899672299457	28.72578806310474	27.77694874381458
45	20.34859220084641	22.812255642239347	29.08894473261665	27.750207424297592
46	20.676053984038774	23.94195701094126	29.996597644615026	25.38539136040494
47	18.653383552894688	23.159057129725245	32.68684601655813	25.500713300821936
48	18.719639947233645	22.374963439602222	31.637846129969976	27.267550483194153
49	19.91750780452573	21.549683342187418	33.13834454518865	25.394464308098204
50	18.548567130859364	21.54586315368499	32.59325139824868	27.312318317206962
51	18.321862819418495	21.305788182485628	29.37999534414526	30.99235365395061
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	681.0
1	1290.5
2	1900.0
3	3905.5
4	5911.0
5	4094.5
6	2278.0
7	2196.5
8	2115.0
9	2134.0
10	2153.0
11	2142.5
12	2132.0
13	2214.0
14	2296.0
15	2151.0
16	2006.0
17	1979.0
18	1952.0
19	2028.5
20	2105.0
21	2636.0
22	3167.0
23	3114.5
24	3062.0
25	4217.0
26	7314.0
27	9256.0
28	10480.0
29	11704.0
30	13322.0
31	14940.0
32	15856.0
33	16772.0
34	18361.5
35	19951.0
36	21729.5
37	23508.0
38	25793.5
39	28079.0
40	31614.5
41	35150.0
42	40137.5
43	45125.0
44	50917.5
45	56710.0
46	67173.5
47	77637.0
48	88716.5
49	99796.0
50	95377.0
51	90958.0
52	75722.5
53	60487.0
54	52468.0
55	44449.0
56	40653.0
57	36857.0
58	34605.0
59	32353.0
60	31058.0
61	29763.0
62	26734.5
63	23706.0
64	20565.5
65	17425.0
66	14027.0
67	10629.0
68	8293.5
69	5958.0
70	5156.0
71	4354.0
72	3465.0
73	2576.0
74	1863.5
75	881.5
76	612.0
77	468.0
78	324.0
79	247.5
80	171.0
81	129.5
82	88.0
83	54.0
84	20.0
85	13.0
86	6.0
87	6.0
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	837655.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.62955505085053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.15177598999219	19.106328015795732
2	12.500685230658162	6.907767415076477
3	4.866559652159839	4.033826334527949
4	2.4355799739308037	2.6917596388188096
5	1.4394583026430283	1.9885796208139703
6	0.9966536688818698	1.6522258446602267
7	0.6616531534945623	1.2796827560332806
8	0.5483906896964305	1.2121432600273132
9	0.4565560263204465	1.1352995876716505
>10	6.385131677243111	42.77803471050192
>50	0.4549966874647741	7.6830556741277505
>100	0.0903909367926788	4.916276709501463
>500	0.0073877207955554795	1.461855389868856
>1k	0.004345718115032635	2.456157195495468
>5k	4.345718115032635E-4	0.6970078470791549
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	5805	0.6930060705182922	RNA PCR Primer, Index 35 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	4150	0.4954306964084259	RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	3458	0.4128191200434546	TruSeq Adapter, Index 10 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3114	0.37175209364237066	No Hit
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1860	0.22204845670353546	RNA PCR Primer, Index 35 (95% over 23bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1489	0.1777581462535292	No Hit
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1427	0.170356531030078	TruSeq Adapter, Index 10 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCC	1334	0.15925410819490124	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1259	0.15030054139233934	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1255	0.14982301782953603	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1110	0.13251278867791633	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	991	0.1183064626845181	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	971	0.11591884487050158	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	955	0.11400875061928838	RNA PCR Primer, Index 35 (95% over 22bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	922	0.11006918122616113	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	859	0.10254818511200912	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	847	0.1011156144235992	TruSeq Adapter, Index 10 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.7752356280330205E-4	0.0	0.0	0.4266673033647504	0.0
2	4.7752356280330205E-4	0.0	0.0	2.0654087900149825	0.0
3	4.7752356280330205E-4	0.0	0.0	2.747789961260901	0.0
4	4.7752356280330205E-4	0.0	0.0	3.5576699237753013	0.0
5	4.7752356280330205E-4	0.0	0.0	5.758217882063618	0.0
6	4.7752356280330205E-4	0.0	0.0	6.894007676191272	0.0
7	4.7752356280330205E-4	0.0	0.0	8.00998024246259	0.0
8	4.7752356280330205E-4	0.0	0.0	9.709009078916738	0.0
9	4.7752356280330205E-4	0.0	0.0	10.29361730067868	0.0
10	4.7752356280330205E-4	0.0	0.0	11.636174797500164	0.0
11	4.7752356280330205E-4	0.0	0.0	13.57766622296769	0.0
12	4.7752356280330205E-4	0.0	0.0	15.020861810649969	0.0
13	4.7752356280330205E-4	0.0	0.0	15.606425079537518	0.0
14	4.7752356280330205E-4	0.0	0.0	15.869421181751438	0.0
15	5.969044535041276E-4	0.0	0.0	16.349212981478054	0.0
16	5.969044535041276E-4	0.0	0.0	17.245763470641254	0.0
17	5.969044535041276E-4	0.0	0.0	18.285451647754744	0.0
18	5.969044535041276E-4	0.0	0.0	19.438909813706122	0.0
19	5.969044535041276E-4	0.0	0.0	20.145286543982905	0.0
20	5.969044535041276E-4	0.0	0.0	20.87756892754177	0.0
21	5.969044535041276E-4	0.0	0.0	21.74857190609499	0.0
22	7.162853442049532E-4	0.0	0.0	22.666491574693637	0.0
23	7.162853442049532E-4	0.0	0.0	23.517438563609122	0.0
24	8.356662349057786E-4	0.0	0.0	24.207818254531997	0.0
25	8.356662349057786E-4	0.0	0.0	24.826330649252974	0.0
26	9.550471256066041E-4	0.0	0.0	25.396255021458714	0.0
27	9.550471256066041E-4	0.0	0.0	25.93167831625192	0.0
28	9.550471256066041E-4	0.0	0.0	26.51222758773003	0.0
29	0.0010744280163074297	0.0	0.0	27.062095970298035	0.0
30	0.0010744280163074297	0.0	0.0	27.71916839271538	0.0
31	0.0010744280163074297	0.0	0.0	28.287302051560605	0.0
32	0.0010744280163074297	0.0	0.0	28.862240421175784	0.0
33	0.0010744280163074297	0.0	0.0	29.44004393216778	0.0
34	0.0010744280163074297	0.0	0.0	29.966036136595616	0.0
35	0.0010744280163074297	0.0	0.0	30.537034936817662	0.0
36	0.0010744280163074297	0.0	0.0	31.117584208295778	0.0
37	0.0010744280163074297	0.0	0.0	31.694313291271467	0.0
38	0.0011938089070082552	0.0	0.0	32.250628241937314	0.0
39	0.0011938089070082552	0.0	0.0	32.756922599399516	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAATA	20	7.0321886E-4	45.000004	45
ATTACCG	20	7.0321886E-4	45.000004	43
TGTTGCG	30	2.1647938E-6	45.000004	1
GTCAACG	20	7.0321886E-4	45.000004	1
ACTCGAA	20	7.0321886E-4	45.000004	39
ATAACGG	40	6.8121153E-9	45.000004	2
TAATGCG	30	2.1647938E-6	45.000004	1
ACGAAAT	20	7.0321886E-4	45.000004	44
CGCGATG	25	3.889897E-5	45.0	29
TCTAGCG	45	3.8380676E-10	45.0	1
ATAACGC	35	1.2113924E-7	45.0	11
AACGGGC	75	0.0	42.0	4
TTTACGG	60	3.6379788E-12	41.250004	2
TAAACGG	60	3.6379788E-12	41.250004	2
TAATCGT	55	6.002665E-11	40.909092	21
CGTTTTT	1890	0.0	40.357143	1
CCCTCGT	140	0.0	40.17857	14
GGCCTAT	90	0.0	40.0	8
GTTCGCG	40	3.4567165E-7	39.375004	1
GCGTATG	40	3.4567165E-7	39.375004	1
>>END_MODULE