FastQCFastQC Report
Sat 18 Jun 2016
SRR3550584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056148
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG106641.0097069728863757No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC85250.807178539371376No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC79300.7508417380897374No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44430.42067967746944557No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC25970.24589356794691652RNA PCR Primer, Index 40 (96% over 27bp)
GAATGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT20410.19324943095096522No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18720.1772478857129872No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGAACCT16830.15935266648234905No Hit
GAACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCT16780.15887924798418404RNA PCR Primer, Index 40 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTA16210.1534822771051027No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15640.14808530622602137No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTC14300.13539769047519856RNA PCR Primer, Index 40 (95% over 21bp)
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA11570.10954904047538792No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11160.10566700879043468No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG253.89059E-545.02
AAGTCGA207.033026E-445.039
CCGATGA20400.043.12518
TACGGCT9950.042.512567
CGTAAGC800.042.187543
CGATGAA22050.041.63265219
CTCGAAT2550.041.4705943
ACGTAGG1150.041.086962
GCGTAAG1050.040.7142871
TGAATGA24400.040.57377222
TAGTGCG501.0822987E-940.51
GATGAAT24950.039.949920
ACCACCG26400.039.80113614
TTGTGCG800.039.3751
GTTAGCG403.4578625E-739.3751
TCGAATG2750.039.27272844
ATGAATG25350.039.2307721
CTACGAA1500.039.011
CGGTCTA750.039.031
TGATACC12950.038.918924
Produced by FastQC (version 0.11.5)