##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550584_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1056148
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19880547044543	33.0	31.0	34.0	30.0	34.0
2	32.344215015319826	34.0	31.0	34.0	30.0	34.0
3	32.30615311490435	34.0	31.0	34.0	30.0	34.0
4	35.90764457254097	37.0	35.0	37.0	35.0	37.0
5	35.973668463132064	37.0	35.0	37.0	35.0	37.0
6	36.01730344610793	37.0	35.0	37.0	35.0	37.0
7	36.069735491616704	37.0	35.0	37.0	35.0	37.0
8	36.02387354802546	37.0	36.0	37.0	35.0	37.0
9	37.80274734222855	39.0	38.0	39.0	35.0	39.0
10	37.55107144074505	39.0	37.0	39.0	35.0	39.0
11	37.48746482500559	39.0	37.0	39.0	35.0	39.0
12	37.41297431799331	39.0	37.0	39.0	35.0	39.0
13	37.372675041755514	39.0	37.0	39.0	34.0	39.0
14	38.693728530471105	40.0	38.0	41.0	35.0	41.0
15	38.74979548320879	40.0	38.0	41.0	35.0	41.0
16	38.8566062710908	40.0	38.0	41.0	35.0	41.0
17	38.725260096122895	40.0	38.0	41.0	35.0	41.0
18	38.66919030287422	40.0	38.0	41.0	35.0	41.0
19	38.60883891272814	40.0	38.0	41.0	35.0	41.0
20	38.34335055314217	40.0	38.0	41.0	34.0	41.0
21	38.41688380795116	40.0	37.0	41.0	34.0	41.0
22	38.38309782341111	40.0	37.0	41.0	34.0	41.0
23	38.228572131936055	40.0	37.0	41.0	34.0	41.0
24	38.24321212557331	40.0	37.0	41.0	34.0	41.0
25	38.284847388812935	40.0	37.0	41.0	34.0	41.0
26	38.23454383287191	40.0	37.0	41.0	34.0	41.0
27	38.10644436196442	40.0	37.0	41.0	34.0	41.0
28	38.072481318906064	40.0	37.0	41.0	34.0	41.0
29	38.05213284501793	40.0	37.0	41.0	34.0	41.0
30	37.95863269163034	40.0	37.0	41.0	33.0	41.0
31	37.87709961103936	40.0	37.0	41.0	33.0	41.0
32	37.631763730083286	40.0	37.0	41.0	33.0	41.0
33	37.498574063483524	40.0	36.0	41.0	33.0	41.0
34	37.37072171703208	40.0	36.0	41.0	32.0	41.0
35	37.24277374004401	40.0	36.0	41.0	32.0	41.0
36	37.22516635926026	40.0	36.0	41.0	32.0	41.0
37	37.16264576555559	40.0	36.0	41.0	32.0	41.0
38	37.06935770365517	40.0	36.0	41.0	31.0	41.0
39	36.942717308559025	40.0	36.0	41.0	31.0	41.0
40	36.93702019035211	40.0	35.0	41.0	31.0	41.0
41	36.871244371054054	40.0	35.0	41.0	31.0	41.0
42	36.81111359392813	40.0	35.0	41.0	31.0	41.0
43	36.745598154804064	40.0	35.0	41.0	31.0	41.0
44	36.746583812117244	40.0	35.0	41.0	31.0	41.0
45	36.704552771013155	40.0	35.0	41.0	31.0	41.0
46	36.57888856486023	40.0	35.0	41.0	30.0	41.0
47	36.474167446229124	39.0	35.0	41.0	30.0	41.0
48	36.476417130932404	39.0	35.0	41.0	30.0	41.0
49	36.426357858936434	39.0	35.0	41.0	30.0	41.0
50	36.31883126228521	39.0	35.0	41.0	30.0	41.0
51	35.481477027840796	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	13.0
9	25.0
10	29.0
11	39.0
12	28.0
13	28.0
14	29.0
15	37.0
16	47.0
17	116.0
18	227.0
19	525.0
20	1315.0
21	2791.0
22	5118.0
23	6742.0
24	7496.0
25	8324.0
26	9489.0
27	10164.0
28	10165.0
29	10709.0
30	12076.0
31	15178.0
32	19326.0
33	25842.0
34	48516.0
35	68475.0
36	60219.0
37	90217.0
38	175972.0
39	466198.0
40	668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.05088302018277	20.800777921276183	22.83325821759829	11.315080840942747
2	30.555282024867726	29.9846233671796	23.262080693236175	16.198013914716498
3	30.673731333108616	28.74616057597988	25.63419142014187	14.945916670769627
4	27.614879732764724	23.785018766309268	31.335475709843696	17.26462579108231
5	29.388210743191294	29.186913197771524	23.37721607198991	18.047659987047272
6	24.869147127107187	38.82410419751777	22.793112328953896	13.513636346421146
7	76.72788283460272	6.2305661706503255	13.166715270965812	3.8748357237811364
8	77.52606642250896	10.755405492412049	8.092047705435224	3.626480379643762
9	71.21482973977132	9.00318894700364	9.320568708173475	10.461412605051565
10	39.203407098247595	31.803781288228546	16.162034108855956	12.830777504667907
11	30.75440184519594	24.88978817362718	26.573264353102026	17.782545628074853
12	26.94991611024212	23.1485549373762	28.51087158239186	21.390657369989814
13	25.731810314463505	23.933672174733086	31.698303646837374	18.636213863966038
14	19.260842230444975	31.336138495741128	28.28116892708219	21.12185034673171
15	19.094198919090886	27.606926301995554	32.80089532906373	20.497979449849833
16	21.598014672186096	26.545806080208457	29.567257619197306	22.28892162840814
17	21.223824691236455	29.329412165719198	28.606123384222666	20.84063975882168
18	21.079810784094654	27.594712104742896	28.601389199241016	22.724087911921433
19	22.439942129322784	27.83558743660926	26.980688312622853	22.7437821214451
20	26.588603112442577	27.125365005662083	29.194298526342898	17.091733355552442
21	23.882637660630895	30.20447891772744	27.84657074576669	18.066312675874972
22	22.630161681885493	25.084552543772276	30.30171907725054	21.983566697091696
23	23.788616746895322	27.52805478020126	29.345224343557913	19.33810412934551
24	21.748845805701475	28.06869870510572	28.759605661327768	21.422849827865033
25	20.898018080799282	31.641304059658303	26.999341001450556	20.461336858091858
26	20.6028889890432	28.67012956517458	29.78626101644845	20.940720429333766
27	23.710313327298827	28.123520567193232	28.11765017781599	20.048515927691952
28	19.065131023303554	30.889894219370774	29.971083598132083	20.0738911591936
29	22.039903498373334	26.3012380840564	31.0942216431788	20.564636774391467
30	20.44845987494177	30.275775743551094	28.63111988092578	20.644644500581357
31	22.78345459159133	28.15126289118571	25.921177713729517	23.144104803493452
32	25.279506281316632	29.132091335684013	26.18051636702432	19.407886015975034
33	23.1485549373762	28.973022720300563	25.50835678332961	22.370065558993627
34	20.854368895268465	28.90958464154645	27.6813476899071	22.554698773277988
35	22.934664459905242	26.220756939368346	27.09942167196264	23.745156928763773
36	20.51521188318304	31.693569461855724	26.047864503838476	21.74335415112276
37	21.922495710828407	28.851448849971785	26.949442691743958	22.27661274745585
38	20.43293174820196	30.137064123588743	25.418407268678255	24.01159685953105
39	23.121001980782996	28.864988619019304	26.407189143945736	21.606820256251964
40	21.003401038490818	28.15012668679011	28.003177584959683	22.84329468975939
41	18.70504891359923	29.421065986963946	27.03162814302541	24.84225695641141
42	20.1315535322701	27.602760219211703	28.38456352708143	23.881122721436768
43	22.30624874544098	26.73053397819245	26.70383317489594	24.259384101470626
44	21.48060688464117	27.717043444668736	27.88614853221329	22.916201138476804
45	21.15271723281207	27.30374909577067	27.255555092657467	24.287978578759795
46	22.616716596537607	29.145631104731535	26.461726954934345	21.775925343796512
47	20.462946480985618	28.90106310857948	29.666675503811966	20.969314906622934
48	21.158398254790047	28.2691440972288	27.62378000053023	22.948677647450925
49	20.70296965955529	26.824838943026926	29.53733757011328	22.934853827304508
50	19.92618458776611	26.927760124528	29.229331495207113	23.91672379249878
51	20.72512564526941	25.75387161647799	27.907452364630714	25.61355037362188
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	590.0
1	1570.5
2	2551.0
3	5615.5
4	8680.0
5	6084.0
6	3488.0
7	3326.0
8	3164.0
9	3076.0
10	2988.0
11	2913.0
12	2838.0
13	2847.0
14	2856.0
15	2684.5
16	2513.0
17	2627.5
18	2742.0
19	2957.5
20	3173.0
21	3526.5
22	3880.0
23	4572.5
24	5265.0
25	6981.0
26	10352.0
27	12007.0
28	15990.0
29	19973.0
30	23419.5
31	26866.0
32	28596.0
33	30326.0
34	33888.0
35	37450.0
36	37711.0
37	37972.0
38	40502.0
39	43032.0
40	48433.5
41	53835.0
42	57263.0
43	60691.0
44	64926.5
45	69162.0
46	89786.5
47	110411.0
48	97967.0
49	85523.0
50	81579.0
51	77635.0
52	71369.5
53	65104.0
54	60610.5
55	56117.0
56	53102.5
57	50088.0
58	46474.0
59	42860.0
60	41445.5
61	40031.0
62	35366.5
63	30702.0
64	25856.5
65	21011.0
66	16725.5
67	12440.0
68	10332.5
69	8225.0
70	6607.5
71	4990.0
72	3936.0
73	2882.0
74	2243.5
75	1240.0
76	875.0
77	688.5
78	502.0
79	375.0
80	248.0
81	172.5
82	97.0
83	69.5
84	42.0
85	27.0
86	12.0
87	8.5
88	5.0
89	2.5
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1056148.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.77931214525001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.17008612023392	17.399343175478673
2	8.93805932602	4.25081805372385
3	3.1606098124409425	2.2547138189811977
4	1.6236893307094182	1.544408616874053
5	1.0149560165601699	1.2067477965741908
6	0.7474955185675589	1.0664957557916104
7	0.6210106507387807	1.0337044276609608
8	0.525597093771309	0.9998669884339357
9	0.48979876649465287	1.0482369981151296
>10	9.118760984725387	53.08955168486974
>50	0.5106611650394788	7.371641744953207
>100	0.06680043472048726	2.948714060027785
>500	0.006036183860284991	0.968774335347113
>1k	0.005231359345580325	2.221941926783876
>5k	8.048245147046655E-4	1.5745932129728115
>10k+	4.0241225735233274E-4	1.0204474034118542
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	10664	1.0097069728863757	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	8525	0.807178539371376	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	7930	0.7508417380897374	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4443	0.42067967746944557	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	2597	0.24589356794691652	RNA PCR Primer, Index 40 (96% over 27bp)
GAATGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT	2041	0.19324943095096522	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1872	0.1772478857129872	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGAACCT	1683	0.15935266648234905	No Hit
GAACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCT	1678	0.15887924798418404	RNA PCR Primer, Index 40 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTA	1621	0.1534822771051027	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1564	0.14808530622602137	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTC	1430	0.13539769047519856	RNA PCR Primer, Index 40 (95% over 21bp)
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	1157	0.10954904047538792	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1116	0.10566700879043468	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.468369963300598E-5	0.0	0.0	0.0711074584243875	0.0
2	9.468369963300598E-5	0.0	0.0	0.4494635221578794	0.0
3	9.468369963300598E-5	0.0	0.0	0.5834409571385829	0.0
4	9.468369963300598E-5	0.0	0.0	0.9132242829603426	0.0
5	9.468369963300598E-5	0.0	0.0	2.2329256884451802	0.0
6	9.468369963300598E-5	0.0	0.0	2.594996155841795	0.0
7	9.468369963300598E-5	0.0	0.0	3.075989349977465	0.0
8	9.468369963300598E-5	0.0	0.0	3.6321614016217425	0.0
9	9.468369963300598E-5	0.0	0.0	3.813480686418949	0.0
10	9.468369963300598E-5	0.0	0.0	5.239795937690551	0.0
11	9.468369963300598E-5	0.0	0.0	5.9390350594803	0.0
12	9.468369963300598E-5	0.0	0.0	7.240841245734499	0.0
13	9.468369963300598E-5	0.0	0.0	7.486072027783985	0.0
14	9.468369963300598E-5	0.0	0.0	7.606036275219004	0.0
15	9.468369963300598E-5	0.0	0.0	7.93288440635214	0.0
16	1.8936739926601195E-4	0.0	0.0	8.299783742430039	0.0
17	2.8405109889901794E-4	0.0	0.0	8.735328760741865	0.0
18	3.787347985320239E-4	0.0	0.0	9.140764362570398	0.0
19	3.787347985320239E-4	0.0	0.0	9.62497680249359	0.0
20	4.734184981650299E-4	0.0	0.0	9.94311403326049	0.0
21	4.734184981650299E-4	0.0	0.0	10.311433624832883	0.0
22	4.734184981650299E-4	0.0	0.0	10.783621234902684	0.0
23	4.734184981650299E-4	0.0	0.0	11.153929184167371	0.0
24	4.734184981650299E-4	0.0	0.0	11.475569711820691	0.0
25	4.734184981650299E-4	0.0	0.0	11.75460257463916	0.0
26	4.734184981650299E-4	0.0	0.0	12.005230327567727	0.0
27	5.681021977980359E-4	0.0	0.0	12.30424145100876	0.0
28	5.681021977980359E-4	0.0	0.0	12.580717853937138	0.0
29	5.681021977980359E-4	0.0	0.0	12.866946677927714	0.0
30	5.681021977980359E-4	0.0	0.0	13.201369505031492	0.0
31	6.627858974310419E-4	0.0	0.0	13.524619655578574	0.0
32	6.627858974310419E-4	0.0	0.0	13.854781716198866	0.0
33	6.627858974310419E-4	0.0	0.0	14.145934092570359	0.0
34	6.627858974310419E-4	0.0	0.0	14.447501675901483	0.0
35	6.627858974310419E-4	0.0	0.0	14.762324977181228	0.0
36	7.574695970640478E-4	0.0	0.0	15.07231940977969	0.0
37	7.574695970640478E-4	0.0	0.0	15.395096141828608	0.0
38	7.574695970640478E-4	0.0	0.0	15.697610562156061	0.0
39	7.574695970640478E-4	0.0	0.0	16.020576661604245	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	25	3.89059E-5	45.0	2
AAGTCGA	20	7.033026E-4	45.0	39
CCGATGA	2040	0.0	43.125	18
TACGGCT	995	0.0	42.51256	7
CGTAAGC	80	0.0	42.1875	43
CGATGAA	2205	0.0	41.632652	19
CTCGAAT	255	0.0	41.47059	43
ACGTAGG	115	0.0	41.08696	2
GCGTAAG	105	0.0	40.714287	1
TGAATGA	2440	0.0	40.573772	22
TAGTGCG	50	1.0822987E-9	40.5	1
GATGAAT	2495	0.0	39.9499	20
ACCACCG	2640	0.0	39.801136	14
TTGTGCG	80	0.0	39.375	1
GTTAGCG	40	3.4578625E-7	39.375	1
TCGAATG	275	0.0	39.272728	44
ATGAATG	2535	0.0	39.23077	21
CTACGAA	150	0.0	39.0	11
CGGTCTA	75	0.0	39.0	31
TGATACC	1295	0.0	38.91892	4
>>END_MODULE