FastQCFastQC Report
Sat 18 Jun 2016
SRR3550583_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550583_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461861
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27030.5852410140713331No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC14280.3091839319622137No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC9900.21435020493178683No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCC8630.18685275440013338No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT8610.18641972368309948No Hit
GGAACCAACTTTTTCTGTATTTTGTATTGTATGTTTTCTTCACCATGTAAC4990.10804116389996124No Hit
TCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC4860.10522646423924081No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGGA253.88717E-545.013
GCAACCT207.0289004E-445.034
CTAAGAT207.0289004E-445.020
CACGACG253.88717E-545.026
TAGCCGT207.0289004E-445.044
TCAATTG253.88717E-545.017
GCGATCA207.0289004E-445.09
ATGCGAT207.0289004E-445.021
CAATGCG207.0289004E-445.01
CGGTCTA253.88717E-545.031
ACGTAGG253.88717E-545.02
CCCATAC253.88717E-545.044
GTAAACG207.0289004E-445.01
CGAAGCA253.88717E-545.014
ACGGTCT253.88717E-545.030
ACGTTAG302.162671E-644.9999961
TTCGTAG302.162671E-644.9999961
CTAGGGT850.042.3529434
CAACGAG1800.041.2514
AGGGCGC603.6379788E-1241.2499966
Produced by FastQC (version 0.11.5)