##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550583_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	461861
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1742428999201	33.0	31.0	34.0	30.0	34.0
2	32.306789272097014	34.0	31.0	34.0	30.0	34.0
3	32.23960455634921	34.0	31.0	34.0	30.0	34.0
4	35.843621782311125	37.0	35.0	37.0	35.0	37.0
5	35.97441654523764	37.0	35.0	37.0	35.0	37.0
6	36.01345426437825	37.0	35.0	37.0	35.0	37.0
7	36.12346138773354	37.0	36.0	37.0	35.0	37.0
8	36.1067355762881	37.0	37.0	37.0	35.0	37.0
9	37.91985250973778	39.0	38.0	39.0	35.0	39.0
10	37.62044424621261	39.0	37.0	39.0	35.0	39.0
11	37.494973595952025	39.0	37.0	39.0	35.0	39.0
12	37.23588915279706	39.0	37.0	39.0	35.0	39.0
13	37.14814846891164	39.0	37.0	39.0	34.0	39.0
14	38.30039124325284	40.0	37.0	41.0	34.0	41.0
15	38.42245827207753	40.0	37.0	41.0	34.0	41.0
16	38.48279027672828	40.0	37.0	41.0	35.0	41.0
17	38.432781724371615	40.0	37.0	41.0	34.0	41.0
18	38.32845379887022	40.0	37.0	41.0	34.0	41.0
19	38.257484394655535	40.0	37.0	41.0	34.0	41.0
20	38.16844245346544	40.0	36.0	41.0	34.0	41.0
21	38.0905012547065	40.0	35.0	41.0	34.0	41.0
22	38.1007359357036	40.0	35.0	41.0	34.0	41.0
23	38.084614202108426	40.0	35.0	41.0	34.0	41.0
24	38.006062430038476	40.0	35.0	41.0	34.0	41.0
25	37.93769770558674	40.0	35.0	41.0	34.0	41.0
26	37.96573860966828	40.0	35.0	41.0	34.0	41.0
27	37.91315135939168	40.0	35.0	41.0	34.0	41.0
28	37.857738583686434	40.0	35.0	41.0	34.0	41.0
29	37.801518205693924	40.0	35.0	41.0	34.0	41.0
30	37.64190308339522	40.0	35.0	41.0	34.0	41.0
31	37.57233886385731	40.0	35.0	41.0	33.0	41.0
32	37.41095914138669	40.0	35.0	41.0	33.0	41.0
33	37.207878560865716	40.0	35.0	41.0	33.0	41.0
34	37.11036870400402	40.0	35.0	41.0	32.0	41.0
35	37.02783954479811	40.0	35.0	41.0	32.0	41.0
36	36.97578708745705	40.0	35.0	41.0	32.0	41.0
37	36.96568231567506	40.0	35.0	41.0	32.0	41.0
38	36.89812302835702	40.0	35.0	41.0	32.0	41.0
39	36.831923457490454	39.0	35.0	41.0	32.0	41.0
40	36.74354405329742	39.0	35.0	41.0	32.0	41.0
41	36.675304907753635	39.0	35.0	41.0	31.0	41.0
42	36.660120685660836	39.0	35.0	41.0	31.0	41.0
43	36.560718484565705	39.0	35.0	41.0	31.0	41.0
44	36.46023370667798	39.0	35.0	41.0	31.0	41.0
45	36.35987667285179	39.0	35.0	41.0	31.0	41.0
46	36.22994797135935	39.0	35.0	41.0	31.0	41.0
47	36.17642537473396	39.0	35.0	41.0	31.0	41.0
48	36.14652460372277	38.0	35.0	41.0	31.0	41.0
49	36.102387947889085	38.0	35.0	41.0	31.0	41.0
50	35.99853419102284	38.0	35.0	41.0	31.0	41.0
51	35.087028348355894	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	10.0
10	15.0
11	10.0
12	14.0
13	16.0
14	20.0
15	20.0
16	34.0
17	61.0
18	95.0
19	189.0
20	342.0
21	526.0
22	824.0
23	1295.0
24	1968.0
25	2941.0
26	4071.0
27	4734.0
28	4964.0
29	5390.0
30	6570.0
31	7946.0
32	10012.0
33	13760.0
34	31138.0
35	46215.0
36	27765.0
37	40443.0
38	78196.0
39	172086.0
40	184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.38582820372363	20.456371072682042	25.257166117078516	12.900634606515812
2	34.738373666536035	23.157833200898107	25.107120973626262	16.996672158939592
3	31.367229534427025	24.23954393204882	27.96079339887975	16.432433134644405
4	29.060258389428856	24.93737293254897	27.47146868863143	18.530899989390747
5	25.308913287764067	29.41924085384997	26.395170841443637	18.876675016942325
6	26.27695345569338	32.69078792104118	26.33389699498334	14.698361628282102
7	77.76733692604485	6.532917912532126	10.879030704042991	4.820714457380034
8	78.15987927103609	6.261624168310378	10.766226202255657	4.812270358397873
9	72.01777158062708	8.171722661146969	12.95064965433323	6.85985610389273
10	42.1873680609534	25.314326171726993	19.062661709908394	13.435644057411212
11	34.47054416805056	23.555788429852274	23.975395194658134	17.998272207439033
12	32.271830702310865	21.049190124301468	28.627660703111975	18.05131847027569
13	27.0395205483901	23.864322815738934	29.42703540675658	19.669121229114385
14	20.444029697246574	26.50884140466504	31.3107190258541	21.736409872234287
15	20.095873000751308	24.484639317890014	36.59087907400711	18.828608607351562
16	24.97807781995016	23.77425242659588	33.21107432755743	18.036595425896536
17	24.579256529561924	24.39954878199285	28.61337068944986	22.40782399899537
18	24.9722319052702	25.225771390093556	30.430367578124155	19.371629126512087
19	25.001894509387025	28.270410361558994	27.76744518372411	18.96024994532987
20	27.00271293744222	27.863144972188604	28.10087883584022	17.03326325452896
21	25.80689861235307	27.235033917130913	28.779221454073845	18.178846016442176
22	22.97660984581941	26.077975841216293	29.294961038061235	21.650453274903057
23	23.344469439939722	25.634119356256534	29.631425905196586	21.389985298607154
24	25.192428024881945	23.614031061293332	30.29136471795627	20.902176195868456
25	22.658548784158004	24.39673408233213	29.168299553328815	23.77641758018105
26	20.638893519911836	26.757184520883985	29.695514451317607	22.908407507886572
27	21.288439595462705	26.78663060964229	30.520004936550176	21.404924858344827
28	19.322696655487256	27.505894630635623	31.18405754112168	21.98735117275544
29	23.304414098614085	26.850502640404798	29.326788795763225	20.518294465217892
30	24.08386938927513	26.639400165850763	29.144699379250465	20.13203106562364
31	25.632603748746916	28.04025453545547	26.5798584422586	19.74728327353901
32	27.60592472627046	26.308564698036857	25.91255810730934	20.172952468383347
33	25.668978328977765	28.157172829054627	26.478745769831185	19.695103072136423
34	22.875713688750512	25.634985417690604	30.19241719911402	21.296883694444865
35	22.019611961174466	28.036573774360683	28.077711692478907	21.866102571985945
36	22.882209149506018	31.842047715654708	26.373302790233428	18.902440344605846
37	24.15900021868051	30.1040789328391	27.33506401276575	18.401856835714643
38	23.041997484091535	29.664769270408197	25.018566191992832	22.27466705350744
39	23.82275186690368	28.09503292116026	26.48892199168148	21.593293220254576
40	23.897666180950548	25.36477425026144	28.860631228876223	21.876928339911792
41	20.010132918778595	26.779269087452718	27.01830204325544	26.19229595051325
42	22.138478893000276	27.296740794308246	26.627058790415298	23.93772152227618
43	23.402495556022266	25.950881325766844	26.274138756032663	24.37248436217823
44	22.251716425504643	26.301203175847277	27.05922344601514	24.387856952632937
45	23.239676006417515	25.40049928441674	26.77580484171645	24.584019867449296
46	22.91078917683026	27.800355518218684	27.143014889761208	22.145840415189852
47	20.693888420975142	27.272058043437315	29.373989143919925	22.66006439166762
48	21.394965151853047	24.670409495497562	29.822175936050023	24.112449416599365
49	23.02640837827831	23.64412669612719	30.37082585453199	22.958639071062507
50	20.578918765602637	24.185848123136612	30.53234631198564	24.702886799275106
51	19.67193592877511	24.355812679572423	28.44015840263629	27.532092989016178
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	450.0
1	912.0
2	1374.0
3	3466.5
4	5559.0
5	3715.0
6	1871.0
7	1734.0
8	1597.0
9	1618.0
10	1639.0
11	1730.5
12	1822.0
13	1824.5
14	1827.0
15	1820.0
16	1813.0
17	1772.0
18	1731.0
19	1665.5
20	1600.0
21	1665.5
22	1731.0
23	2008.0
24	2285.0
25	2608.0
26	3321.5
27	3712.0
28	4589.5
29	5467.0
30	6189.5
31	6912.0
32	7417.5
33	7923.0
34	9541.0
35	11159.0
36	11640.0
37	12121.0
38	13173.0
39	14225.0
40	16202.5
41	18180.0
42	21502.0
43	24824.0
44	27079.0
45	29334.0
46	32781.0
47	36228.0
48	39258.5
49	42289.0
50	42639.5
51	42990.0
52	39287.0
53	35584.0
54	32965.0
55	30346.0
56	27756.0
57	25166.0
58	23946.0
59	22726.0
60	22526.0
61	22326.0
62	19760.0
63	17194.0
64	14201.0
65	11208.0
66	8451.0
67	5694.0
68	4425.0
69	3156.0
70	2553.0
71	1950.0
72	1737.5
73	1525.0
74	1084.5
75	528.5
76	413.0
77	311.5
78	210.0
79	130.5
80	51.0
81	56.0
82	61.0
83	33.5
84	6.0
85	4.5
86	3.0
87	3.0
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	461861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.18743981361898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.04083850716148	22.779567548783625
2	10.68501523975815	6.664765393951176
3	3.7502613413600847	3.508831496070288
4	1.782380441547192	2.223515309828987
5	1.0596337945362246	1.65236325957876
6	0.7744335344517487	1.4491559547217279
7	0.6180699783381026	1.3493214175017052
8	0.5131162017900157	1.2802224528575106
9	0.4670120339639189	1.310841873133695
>10	7.009328545062704	49.04843019203253
>50	0.251201039241179	4.849247088638726
>100	0.04523010401849483	2.3982559667284202
>500	0.002087543262392069	0.5889844080808011
>1k	0.0013916955082613795	0.8964976380920374
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2703	0.5852410140713331	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	1428	0.3091839319622137	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	990	0.21435020493178683	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCC	863	0.18685275440013338	No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT	861	0.18641972368309948	No Hit
GGAACCAACTTTTTCTGTATTTTGTATTGTATGTTTTCTTCACCATGTAAC	499	0.10804116389996124	No Hit
TCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	486	0.10522646423924081	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.660614340678256E-4	0.0	0.0	0.1926986690800912	0.0
2	8.660614340678256E-4	0.0	0.0	0.9282013419621921	0.0
3	8.660614340678256E-4	0.0	0.0	1.281987437778899	0.0
4	8.660614340678256E-4	0.0	0.0	1.6899023732248446	0.0
5	8.660614340678256E-4	0.0	0.0	2.9355152307728947	0.0
6	8.660614340678256E-4	0.0	0.0	3.5382939888841016	0.0
7	8.660614340678256E-4	0.0	0.0	4.208842054211115	0.0
8	8.660614340678256E-4	0.0	0.0	5.202647549803945	0.0
9	8.660614340678256E-4	0.0	0.0	5.606665208796586	0.0
10	8.660614340678256E-4	0.0	0.0	6.574705376725898	0.0
11	8.660614340678256E-4	0.0	0.0	8.18514661337502	0.0
12	8.660614340678256E-4	0.0	0.0	9.337874382119296	0.0
13	8.660614340678256E-4	0.0	0.0	9.79645391145821	0.0
14	8.660614340678256E-4	0.0	0.0	9.994998495218258	0.0
15	8.660614340678256E-4	0.0	0.0	10.41395571394857	0.0
16	8.660614340678256E-4	0.0	0.0	11.168078707663128	0.0
17	8.660614340678256E-4	0.0	0.0	12.024829981314724	0.0
18	8.660614340678256E-4	0.0	0.0	12.941772524634034	0.0
19	0.001082576792584782	0.0	0.0	13.570533125767277	0.0
20	0.001082576792584782	0.0	0.0	14.190633112559839	0.0
21	0.001082576792584782	0.0	0.0	14.917475171101263	0.0
22	0.001082576792584782	0.0	0.0	15.690651516365314	0.0
23	0.001082576792584782	0.0	0.0	16.3417131994258	0.0
24	0.001082576792584782	0.0	0.0	16.916994507005356	0.0
25	0.001082576792584782	0.0	0.0	17.426888176312787	0.0
26	0.001082576792584782	0.0	0.0	17.88633376708577	0.0
27	0.001082576792584782	0.0	0.0	18.339067381744723	0.0
28	0.001082576792584782	0.0	0.0	18.812369955462792	0.0
29	0.001082576792584782	0.0	0.0	19.278744037708314	0.0
30	0.001082576792584782	0.0	0.0	19.80357726675342	0.0
31	0.001082576792584782	0.0	0.0	20.29723228417208	0.0
32	0.001082576792584782	0.0	0.0	20.764039397134635	0.0
33	0.001082576792584782	0.0	0.0	21.270685336064314	0.0
34	0.001082576792584782	0.0	0.0	21.754380646991194	0.0
35	0.001082576792584782	0.0	0.0	22.271419323129685	0.0
36	0.001082576792584782	0.0	0.0	22.755980695490635	0.0
37	0.001082576792584782	0.0	0.0	23.2292832692087	0.0
38	0.001082576792584782	0.0	0.0	23.75649816719749	0.0
39	0.001082576792584782	0.0	0.0	24.35537964885539	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGGA	25	3.88717E-5	45.0	13
GCAACCT	20	7.0289004E-4	45.0	34
CTAAGAT	20	7.0289004E-4	45.0	20
CACGACG	25	3.88717E-5	45.0	26
TAGCCGT	20	7.0289004E-4	45.0	44
TCAATTG	25	3.88717E-5	45.0	17
GCGATCA	20	7.0289004E-4	45.0	9
ATGCGAT	20	7.0289004E-4	45.0	21
CAATGCG	20	7.0289004E-4	45.0	1
CGGTCTA	25	3.88717E-5	45.0	31
ACGTAGG	25	3.88717E-5	45.0	2
CCCATAC	25	3.88717E-5	45.0	44
GTAAACG	20	7.0289004E-4	45.0	1
CGAAGCA	25	3.88717E-5	45.0	14
ACGGTCT	25	3.88717E-5	45.0	30
ACGTTAG	30	2.162671E-6	44.999996	1
TTCGTAG	30	2.162671E-6	44.999996	1
CTAGGGT	85	0.0	42.352943	4
CAACGAG	180	0.0	41.25	14
AGGGCGC	60	3.6379788E-12	41.249996	6
>>END_MODULE