FastQCFastQC Report
Sat 18 Jun 2016
SRR3550580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686786
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCG43480.6330938603873695No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42490.6186788897851733No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC41980.6112529958385873No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTC41130.5988765059276107No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC20030.291648344608073No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT18130.2639832495129487No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCC14060.20472170370391943No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12940.18841385817416198No Hit
CCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC11340.16511693598879418No Hit
CTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGCT10790.157108618987574No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9910.14429531178562172No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9770.14225683109440204No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCT8540.12434732216440056No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA8320.12114399536391249No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8090.11779506279976587No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACAACCATCGTA8010.11663021669049747No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACAACCAT7330.10672902476171617No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCC207.031301E-445.019
CGATTCG207.031301E-445.010
CTAAGCG253.889161E-545.01
CGTTATG253.889161E-545.011
CTTGCGT253.889161E-545.015
ACATGCG207.031301E-445.01
CATCGAA453.8380676E-1045.042
CGGTCTA406.8084773E-945.031
CGTCACG253.889161E-545.036
ATACCGG207.031301E-445.02
GCGACTA302.1642209E-644.9999961
CCGATGA4450.040.9550618
TACGGGT556.002665E-1140.9090924
CGTTTTT23200.040.732761
CGCATCG501.0804797E-940.521
ACGCTAG501.0804797E-940.51
ATTGGGC1000.040.54
CGATGAA4850.040.36082519
GGTATGC1450.040.344838
ACGGGTA950.040.263165