##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550580_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686786
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12699443494771	33.0	31.0	34.0	30.0	34.0
2	32.26109151904669	33.0	31.0	34.0	30.0	34.0
3	32.23739418101126	34.0	31.0	34.0	30.0	34.0
4	35.83345175935444	37.0	35.0	37.0	35.0	37.0
5	35.939768719805	37.0	35.0	37.0	35.0	37.0
6	35.97863963447129	37.0	35.0	37.0	35.0	37.0
7	36.04211064290769	37.0	35.0	37.0	35.0	37.0
8	35.99621861831779	37.0	35.0	37.0	35.0	37.0
9	37.800841892525476	39.0	38.0	39.0	35.0	39.0
10	37.52758646798275	39.0	37.0	39.0	35.0	39.0
11	37.4605277335298	39.0	37.0	39.0	35.0	39.0
12	37.340418995145505	39.0	37.0	39.0	35.0	39.0
13	37.28886145029165	39.0	37.0	39.0	34.0	39.0
14	38.53755463856281	40.0	38.0	41.0	34.0	41.0
15	38.62520057193944	40.0	38.0	41.0	35.0	41.0
16	38.71055903876899	40.0	38.0	41.0	35.0	41.0
17	38.63344185816251	40.0	38.0	41.0	35.0	41.0
18	38.54486259184083	40.0	38.0	41.0	35.0	41.0
19	38.468888416479075	40.0	37.0	41.0	34.0	41.0
20	38.28414964777966	40.0	37.0	41.0	34.0	41.0
21	38.2570800802579	40.0	37.0	41.0	34.0	41.0
22	38.25169121094489	40.0	37.0	41.0	34.0	41.0
23	38.191226961528045	40.0	37.0	41.0	34.0	41.0
24	38.116328812759726	40.0	36.0	41.0	34.0	41.0
25	38.10453620196102	40.0	36.0	41.0	34.0	41.0
26	38.09506891520794	40.0	36.0	41.0	34.0	41.0
27	38.001613311861334	40.0	36.0	41.0	34.0	41.0
28	37.96584962419153	40.0	36.0	41.0	34.0	41.0
29	37.90609447484369	40.0	36.0	41.0	34.0	41.0
30	37.7922424161238	40.0	36.0	41.0	33.0	41.0
31	37.7389638111435	40.0	36.0	41.0	33.0	41.0
32	37.50011066038038	40.0	36.0	41.0	33.0	41.0
33	37.355974058877145	40.0	35.0	41.0	33.0	41.0
34	37.28540622552003	40.0	35.0	41.0	33.0	41.0
35	37.119727251283514	40.0	35.0	41.0	32.0	41.0
36	37.06590553680477	40.0	35.0	41.0	32.0	41.0
37	36.944254833383326	40.0	35.0	41.0	32.0	41.0
38	36.94568322592482	40.0	35.0	41.0	31.0	41.0
39	36.86461721700792	40.0	35.0	41.0	31.0	41.0
40	36.78064054887549	40.0	35.0	41.0	31.0	41.0
41	36.70341125183099	40.0	35.0	41.0	31.0	41.0
42	36.64728022994062	40.0	35.0	41.0	31.0	41.0
43	36.54566779171387	39.0	35.0	41.0	31.0	41.0
44	36.49822652179864	39.0	35.0	41.0	31.0	41.0
45	36.423884587047496	39.0	35.0	41.0	30.0	41.0
46	36.30946903402224	39.0	35.0	41.0	30.0	41.0
47	36.198488320961694	39.0	35.0	41.0	30.0	41.0
48	36.156861089189356	39.0	35.0	41.0	30.0	41.0
49	36.103029182307154	39.0	35.0	41.0	30.0	41.0
50	36.002355901255996	39.0	35.0	41.0	30.0	41.0
51	35.075882443730656	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	8.0
9	22.0
10	30.0
11	16.0
12	17.0
13	25.0
14	25.0
15	24.0
16	51.0
17	84.0
18	176.0
19	310.0
20	653.0
21	1275.0
22	2071.0
23	2952.0
24	3870.0
25	5269.0
26	6665.0
27	7201.0
28	7551.0
29	7796.0
30	8919.0
31	11150.0
32	14120.0
33	19278.0
34	36547.0
35	53500.0
36	43333.0
37	63966.0
38	117371.0
39	272138.0
40	370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.121854551490564	21.407978613425435	23.672876267134157	12.797290567949842
2	30.92506253767549	28.35395596299284	23.795767531661973	16.9252139676697
3	32.0690870227407	26.33076387695731	26.23306240954242	15.367086690759567
4	28.318719368187473	24.912418133159385	29.462452641725367	17.306409856927775
5	27.55428910898009	29.82268130101662	23.90074928725979	18.722280302743506
6	24.487831726331056	37.31482587006724	24.630088557425456	13.567253846176246
7	75.37122189444747	7.309846152950119	12.792194366221793	4.526737586380619
8	75.37034825986552	10.006319290142782	10.08582003710035	4.537512412891352
9	68.72708529294424	9.980692675738876	11.966172868986845	9.32604916233004
10	40.20495467292577	27.72202694871474	18.55308640537227	13.519931972987218
11	31.78981516804361	25.40340076821601	24.45841936207203	18.348364701668352
12	28.720882487412382	22.745076341101885	28.293529571074544	20.24051160041119
13	25.563567108240413	25.607540048865296	29.7806012353193	19.048291607574992
14	20.057921972783372	29.93800106583419	28.299790618911857	21.70428634247058
15	20.07102649151264	26.693176622703437	33.18238869167397	20.053408194109956
16	22.240697975788674	27.367476914206172	29.36242148209195	21.029403627913208
17	22.075872251327198	29.006269784183136	27.416982873850078	21.50087509063959
18	21.971327313020357	28.496649611378217	28.77009723552897	20.761925840072454
19	23.93234573797369	28.672250162350426	26.322027531137792	21.073376568538087
20	24.969204380986216	28.336046454062835	28.7067587283375	17.987990436613444
21	23.47106667870341	30.138208990864694	28.092739222989405	18.297985107442493
22	21.853241038693273	26.5724694446305	28.703846613064332	22.870442903611895
23	23.486500889651214	27.372281904406904	28.239073015466243	20.902144190475635
24	22.40348521955893	27.563753483617898	28.42209946038504	21.610661836438133
25	21.503641600149102	30.70854676711523	26.35784654899779	21.429965083737876
26	20.261187618850705	29.28306634089804	28.00348288986671	22.452263150384542
27	22.689018122093348	28.80810033984385	28.145448509433795	20.357433028629004
28	19.092264548199875	29.849618367293452	28.735297458014582	22.322819626492098
29	22.208227890492818	27.48206865020545	28.414673566438452	21.89502989286328
30	20.927333987588565	31.33872851222943	27.20206876669006	20.531868733491944
31	23.330993934063887	28.539457705893827	24.58349471305472	23.546053646987563
32	23.727332822742454	31.020143101344523	24.44910059319788	20.80342348271514
33	21.788155262337906	31.124105616596726	24.3208219154147	22.766917205650667
34	21.163943353533707	29.01515173576631	25.52017659067016	24.300728320029823
35	22.45371920802113	29.263991985858766	24.860873692824256	23.421415113295847
36	21.619689393784963	32.60433380994953	23.847603183524416	21.928373612741087
37	19.702498303692852	32.3441363102917	26.03241766722094	21.9209477187945
38	19.595769278931137	31.819373138066297	24.152356046861758	24.43250153614081
39	21.627406499258868	30.231396679606164	25.660686152600665	22.480510668534304
40	21.47641332234495	27.99678502473842	26.741663342001733	23.7851383109149
41	19.22301852396525	29.14910903833217	25.31312519474771	26.314747242954866
42	19.992399379137023	28.604106664958223	26.439240170882922	24.964253785021825
43	21.77534195513595	28.986321794561913	25.663598267873834	23.574737982428296
44	20.70426595766367	28.342744319191134	26.3153296660095	24.6376600571357
45	21.114000576598823	27.976837035117196	25.283712830488682	25.625449557795292
46	22.397952200539905	30.705634651842058	24.713811871529124	22.182601276088914
47	20.049331232727514	28.070898358440623	28.68302498886116	23.196745419970703
48	20.174260977946552	28.502765053451878	27.265844091172504	24.057129877429066
49	20.507698176724627	27.428048911888126	28.449618949716506	23.61463396167074
50	19.952794611421897	27.441735853672032	27.676015527398633	24.929454007507434
51	20.250267186576313	26.279365042385837	26.484377957617074	26.985989813420773
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	640.0
1	1217.0
2	1794.0
3	4555.5
4	7317.0
5	4939.5
6	2562.0
7	2496.5
8	2431.0
9	2571.0
10	2711.0
11	2793.5
12	2876.0
13	2801.5
14	2727.0
15	2640.0
16	2553.0
17	2484.5
18	2416.0
19	2376.0
20	2336.0
21	2480.0
22	2624.0
23	2717.5
24	2811.0
25	3891.0
26	6014.5
27	7058.0
28	8967.5
29	10877.0
30	12528.0
31	14179.0
32	15033.5
33	15888.0
34	17274.5
35	18661.0
36	20403.0
37	22145.0
38	23568.0
39	24991.0
40	28590.5
41	32190.0
42	35108.5
43	38027.0
44	41707.5
45	45388.0
46	58143.5
47	70899.0
48	66632.5
49	62366.0
50	60353.0
51	58340.0
52	51598.5
53	44857.0
54	40636.5
55	36416.0
56	34036.5
57	31657.0
58	29128.0
59	26599.0
60	25696.5
61	24794.0
62	21839.0
63	18884.0
64	16480.0
65	14076.0
66	11931.0
67	9786.0
68	8148.0
69	6510.0
70	5460.5
71	4411.0
72	3743.0
73	3075.0
74	2417.5
75	1463.0
76	1166.0
77	876.5
78	587.0
79	381.5
80	176.0
81	161.0
82	146.0
83	101.5
84	57.0
85	35.0
86	13.0
87	9.0
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	15.0
96	30.0
97	15.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	686786.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.59721297002073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.70671959136213	22.85817409628527
2	10.004373241048999	6.122118773759055
3	3.4258603545231776	3.144653366186892
4	1.6851371123083068	2.06241996435932
5	1.0472598285980959	1.6021616005281676
6	0.7608342089765278	1.3967643796159248
7	0.6021971148537553	1.2897887361178673
8	0.4916215346800112	1.2033799037802189
9	0.4226952695418567	1.1639967465233259
>10	6.524839728497514	45.88454131827501
>50	0.25848532532837454	4.8363911393919015
>100	0.05902795642777452	3.3141769006004984
>500	0.006188414786782812	1.3873400271697172
>1k	0.004760319066756009	3.734093047406829
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCG	4348	0.6330938603873695	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4249	0.6186788897851733	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC	4198	0.6112529958385873	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTC	4113	0.5988765059276107	No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	2003	0.291648344608073	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1813	0.2639832495129487	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCC	1406	0.20472170370391943	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1294	0.18841385817416198	No Hit
CCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	1134	0.16511693598879418	No Hit
CTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGCT	1079	0.157108618987574	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	991	0.14429531178562172	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	977	0.14225683109440204	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCT	854	0.12434732216440056	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	832	0.12114399536391249	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	809	0.11779506279976587	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACAACCATCGTA	801	0.11663021669049747	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACAACCAT	733	0.10672902476171617	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4560576365854865E-4	0.0	0.0	0.16744662820733097	0.0
2	1.4560576365854865E-4	0.0	0.0	0.760062086297624	0.0
3	1.4560576365854865E-4	0.0	0.0	1.0030781058437417	0.0
4	1.4560576365854865E-4	0.0	0.0	1.3567545057703565	0.0
5	1.4560576365854865E-4	0.0	0.0	2.7386988086536417	0.0
6	1.4560576365854865E-4	0.0	0.0	3.25254154860466	0.0
7	1.4560576365854865E-4	0.0	0.0	3.898011898903006	0.0
8	1.4560576365854865E-4	0.0	0.0	4.844740574210889	0.0
9	1.4560576365854865E-4	0.0	0.0	5.205988473847749	0.0
10	1.4560576365854865E-4	0.0	0.0	6.617781958281037	0.0
11	1.4560576365854865E-4	0.0	0.0	7.9074122070048025	0.0
12	1.4560576365854865E-4	0.0	0.0	9.461025705241516	0.0
13	1.4560576365854865E-4	0.0	0.0	9.860422314956915	0.0
14	1.4560576365854865E-4	0.0	0.0	10.051020259585956	0.0
15	2.912115273170973E-4	0.0	0.0	10.465414262958184	0.0
16	2.912115273170973E-4	0.0	0.0	11.052351096265795	0.0
17	2.912115273170973E-4	0.0	0.0	11.753151636754389	0.0
18	4.3681729097564596E-4	0.0	0.0	12.492683310376158	0.0
19	4.3681729097564596E-4	0.0	0.0	13.097384046850111	0.0
20	4.3681729097564596E-4	0.0	0.0	13.625932968930643	0.0
21	5.824230546341946E-4	0.0	0.0	14.231798551513863	0.0
22	5.824230546341946E-4	0.0	0.0	14.916000034945384	0.0
23	5.824230546341946E-4	0.0	0.0	15.533805290148605	0.0
24	5.824230546341946E-4	0.0	0.0	16.052307414536696	0.0
25	5.824230546341946E-4	0.0	0.0	16.50252043576893	0.0
26	5.824230546341946E-4	0.0	0.0	16.923175486978476	0.0
27	5.824230546341946E-4	0.0	0.0	17.343248115133388	0.0
28	7.280288182927433E-4	0.0	0.0	17.786908877000986	0.0
29	7.280288182927433E-4	0.0	0.0	18.24192688843395	0.0
30	7.280288182927433E-4	0.0	0.0	18.736404061818384	0.0
31	7.280288182927433E-4	0.0	0.0	19.20117765941647	0.0
32	7.280288182927433E-4	0.0	0.0	19.675561237416023	0.0
33	7.280288182927433E-4	0.0	0.0	20.106699903608984	0.0
34	7.280288182927433E-4	0.0	0.0	20.540750685075118	0.0
35	8.736345819512919E-4	0.0	0.0	21.01906561869345	0.0
36	0.0010192403456098407	0.0	0.0	21.489081023783246	0.0
37	0.0010192403456098407	0.0	0.0	21.968852015038163	0.0
38	0.0010192403456098407	0.0	0.0	22.428966228199176	0.0
39	0.0010192403456098407	0.0	0.0	22.92227855547434	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCC	20	7.031301E-4	45.0	19
CGATTCG	20	7.031301E-4	45.0	10
CTAAGCG	25	3.889161E-5	45.0	1
CGTTATG	25	3.889161E-5	45.0	11
CTTGCGT	25	3.889161E-5	45.0	15
ACATGCG	20	7.031301E-4	45.0	1
CATCGAA	45	3.8380676E-10	45.0	42
CGGTCTA	40	6.8084773E-9	45.0	31
CGTCACG	25	3.889161E-5	45.0	36
ATACCGG	20	7.031301E-4	45.0	2
GCGACTA	30	2.1642209E-6	44.999996	1
CCGATGA	445	0.0	40.95506	18
TACGGGT	55	6.002665E-11	40.909092	4
CGTTTTT	2320	0.0	40.73276	1
CGCATCG	50	1.0804797E-9	40.5	21
ACGCTAG	50	1.0804797E-9	40.5	1
ATTGGGC	100	0.0	40.5	4
CGATGAA	485	0.0	40.360825	19
GGTATGC	145	0.0	40.34483	8
ACGGGTA	95	0.0	40.26316	5
>>END_MODULE