FastQCFastQC Report
Sat 18 Jun 2016
SRR3550576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805275
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42100.5228027692403217No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10200.12666480394896154No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC9280.11524013535748658TruSeq Adapter, Index 20 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCA253.889761E-545.016
TAGAACG207.0320274E-445.01
CGCGATT207.0320274E-445.011
CGCTGTT253.889761E-545.013
AATAGCG207.0320274E-445.01
TATCGCG351.2113196E-745.01
TCGAACG253.889761E-545.01
CTACGAA1300.041.5384611
TCGAAGG501.0804797E-940.52
GTTAGCG950.040.2631571
TACGAAT1350.040.012
TTAACGG451.9270374E-840.02
CGTTTTT18750.039.9600031
CGAATAT1400.038.57142614
TACACGG700.038.5714262
TACGTTC356.2459603E-638.57142631
GCGAGAC1300.038.0769221
GACACGA1300.038.0769225
CTACGGG1550.037.7419363
CGCATCT301.1396181E-437.50000412
Produced by FastQC (version 0.11.5)