FastQCFastQC Report
Sat 18 Jun 2016
SRR3550570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790943
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94981.200845067217233No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC28510.3604558103428439No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG27140.3431347138795084No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC19590.24767903628959356TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC18690.23630021379543154No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCC17780.22479495994022328No Hit
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT16120.20380735400654665TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC10980.1388216344287768TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCT9390.11871904802242388No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTC8240.10417944150210572No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTT8020.10139795155908833No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACC207.031951E-445.033
GACACGA453.8380676E-1045.025
ACGCTAG207.031951E-445.01
TAGGCCG207.031951E-445.01
CGATAGC207.031951E-445.010
TAGTACG406.8102963E-945.01
CGTTTTT50200.043.5657351
CGTAAGG403.456389E-739.3752
ACGTTAG750.039.01
CGCGGGT356.2458184E-638.5714264
TCTAGCG356.2458184E-638.5714261
CGACTAT356.2458184E-638.57142645
CGATACG356.2458184E-638.57142610
GTACGAG356.2458184E-638.5714261
TTGCGAG659.094947E-1238.076921
AATACGG659.094947E-1238.076922
TACGGGA3850.037.987014
GCTACGA900.037.510
GCGCGAC601.546141E-1037.4999969
CGACAGG601.546141E-1037.4999962
Produced by FastQC (version 0.11.5)