FastQCFastQC Report
Sat 18 Jun 2016
SRR3550569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635748
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42980.6760540339883098No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC18330.2883217878782159No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC14200.22335894096402975No Hit
GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC9440.14848650723242543No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8540.13432995463611366No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT7500.11797127163593123No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG302.1639644E-645.0000041
GCGCGAC302.1639644E-645.0000049
TGCAACG351.2107739E-745.0000041
CGTATGG551.8189894E-1245.02
CGACGGT406.8066583E-945.028
CGCATAC253.888832E-545.026
GCGCTTA207.030905E-445.01
TGTCGCG253.888832E-545.01
TCGCGAG253.888832E-545.01
AATCGCG207.030905E-445.01
CGTTTTT23400.042.9807661
GCTACGA1050.042.85714710
CGCACGG700.041.7857172
CGGGTCC451.925946E-840.06
CGGGTAT451.925946E-840.06
ACGATAG451.925946E-840.01
ACGGTCT451.925946E-840.030
GCGAGAC403.4549703E-739.37521
ACACGAC403.4549703E-739.37526
CACGACC403.4549703E-739.37527