##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550569_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	635748
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24333383667742	33.0	31.0	34.0	30.0	34.0
2	32.37981401435789	34.0	31.0	34.0	30.0	34.0
3	32.31484959449342	34.0	31.0	34.0	30.0	34.0
4	35.8913767719285	37.0	35.0	37.0	35.0	37.0
5	36.02175547543995	37.0	35.0	37.0	35.0	37.0
6	36.075059614815935	37.0	35.0	37.0	35.0	37.0
7	36.21524094452519	37.0	37.0	37.0	35.0	37.0
8	36.227854432888506	37.0	37.0	37.0	35.0	37.0
9	38.048898305617946	39.0	39.0	39.0	37.0	39.0
10	37.68610046748083	39.0	38.0	39.0	35.0	39.0
11	37.56057116970875	39.0	37.0	39.0	35.0	39.0
12	37.586507547015486	39.0	37.0	39.0	35.0	39.0
13	37.61437236137589	39.0	37.0	39.0	35.0	39.0
14	38.91899306014333	40.0	38.0	41.0	35.0	41.0
15	39.02678577046251	40.0	38.0	41.0	35.0	41.0
16	39.063039128711374	40.0	38.0	41.0	35.0	41.0
17	39.01991197770186	40.0	38.0	41.0	35.0	41.0
18	38.93005090067133	40.0	38.0	41.0	35.0	41.0
19	38.84858465933043	40.0	38.0	41.0	35.0	41.0
20	38.77254981533564	40.0	38.0	41.0	35.0	41.0
21	38.7032110207189	40.0	38.0	41.0	35.0	41.0
22	38.705192938082384	40.0	38.0	41.0	35.0	41.0
23	38.68052121280759	40.0	38.0	41.0	35.0	41.0
24	38.611740815543264	40.0	38.0	41.0	35.0	41.0
25	38.549653321756416	40.0	38.0	41.0	35.0	41.0
26	38.59509900149116	40.0	38.0	41.0	35.0	41.0
27	38.549864097095075	40.0	38.0	41.0	35.0	41.0
28	38.52966898834129	40.0	38.0	41.0	35.0	41.0
29	38.47842541384322	40.0	38.0	41.0	35.0	41.0
30	38.36284030779491	40.0	38.0	41.0	34.0	41.0
31	38.310166921484615	40.0	38.0	41.0	34.0	41.0
32	38.161425596305456	40.0	37.0	41.0	34.0	41.0
33	38.023106639737755	40.0	37.0	41.0	34.0	41.0
34	37.95661016629231	40.0	37.0	41.0	34.0	41.0
35	37.88160717768675	40.0	37.0	41.0	34.0	41.0
36	37.78415661551432	40.0	37.0	41.0	33.0	41.0
37	37.7244411307625	40.0	37.0	41.0	33.0	41.0
38	37.68321410370147	40.0	37.0	41.0	33.0	41.0
39	37.658359916193206	40.0	37.0	41.0	33.0	41.0
40	37.59597513480184	40.0	37.0	41.0	33.0	41.0
41	37.52952113101418	40.0	37.0	41.0	33.0	41.0
42	37.49137551356827	40.0	37.0	41.0	33.0	41.0
43	37.38770236005461	40.0	36.0	41.0	33.0	41.0
44	37.32877335044703	40.0	36.0	41.0	33.0	41.0
45	37.26336221270063	40.0	36.0	41.0	33.0	41.0
46	37.175254975241764	40.0	36.0	41.0	32.0	41.0
47	37.12749233973209	40.0	36.0	41.0	32.0	41.0
48	37.07695659286384	40.0	35.0	41.0	32.0	41.0
49	37.02738663747271	40.0	35.0	41.0	32.0	41.0
50	36.95832782800732	40.0	35.0	41.0	32.0	41.0
51	36.08270258026765	39.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	16.0
10	19.0
11	16.0
12	18.0
13	14.0
14	20.0
15	18.0
16	28.0
17	42.0
18	75.0
19	148.0
20	283.0
21	467.0
22	732.0
23	1232.0
24	1981.0
25	3316.0
26	4732.0
27	5649.0
28	5909.0
29	6200.0
30	6788.0
31	8330.0
32	10567.0
33	14308.0
34	26827.0
35	40784.0
36	35470.0
37	53157.0
38	110824.0
39	297352.0
40	421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.87293707569666	23.056305328526395	28.32065535400819	13.750102241768753
2	32.024638693318735	25.223830826050573	26.692651805432345	16.058878675198347
3	29.917199896814463	23.792603358563454	30.452160289926194	15.838036454695887
4	27.533079144566713	25.296029244291763	29.57130812837791	17.599583482763613
5	25.02579638473106	29.567061162599018	27.36351510346867	18.043627349201255
6	26.38325248368851	32.732466323134325	25.88557101241372	14.998710180763448
7	81.24303969497349	5.006700768228921	9.544347760433379	4.2059117763642195
8	84.08064201538976	4.3210517374808886	8.228732139149475	3.3695741079798913
9	77.5156193963646	8.079931041859354	10.051309638410187	4.353139923365862
10	33.1178391438117	41.53595449769406	15.88664061860989	9.459565739884358
11	22.30427779560455	23.706091092697108	36.299288397289494	17.690342714408853
12	22.590554748107742	22.12684900306411	37.121941398164054	18.1606548506641
13	21.376866305517282	24.01265910392168	37.048641914720925	17.561832675840115
14	17.91763403109408	27.683295897116466	34.73734876082976	19.66172131095969
15	17.183538131460892	28.127182468525262	36.698031295418936	17.991248104594902
16	20.773954459943248	27.39497410923825	33.98170344224441	17.849367988574087
17	20.434983672776006	27.099888635119573	31.339776137714942	21.125351554389475
18	20.578594034114143	27.167682792553023	33.31697465033315	18.93674852299968
19	20.055116178108307	27.809918395339032	32.69754682673009	19.43741859982257
20	21.842616885935936	28.497140376375548	32.13411603339688	17.526126704291638
21	21.77246330306977	27.872207226762807	32.7678577046251	17.587471765542322
22	20.833097390790062	25.26236810811831	32.42778585225592	21.476748648835702
23	19.022002428635247	27.440432372575295	32.66011060986428	20.877454588925172
24	19.582601911449192	27.29682201123716	33.07945915677281	20.041116920540844
25	18.87729100209517	28.825729691638823	30.745358223698698	21.55162108256731
26	18.94948942033636	30.096358934672228	30.733561096535105	20.220590548456308
27	19.60729723097831	28.8463353404179	32.31327507125465	19.23309235734914
28	17.775753915073267	28.151563197996694	33.91626871024368	20.15641417668636
29	20.33997747535187	25.64176371769947	32.95330854363679	21.06495026331188
30	20.711508333490627	26.040349320800065	33.24619188735159	20.001950458357715
31	21.35280016610355	27.66096000302006	31.058532626134884	19.927707204741502
32	21.95917250231224	28.339845347527636	29.904301704448933	19.796680445711193
33	21.113082542139338	27.548494057393807	30.125930400095633	21.212493000371214
34	20.439859818670293	26.321435537351277	31.87615218608631	21.36255245789212
35	21.123306719014455	27.108697156735058	30.61228662929337	21.15570949495712
36	21.28201740312199	27.19143434190906	31.651062999804953	19.875485255163998
37	20.501834060036366	27.512158905729944	33.009620163964335	18.97638687026935
38	20.249060948677776	28.340002642556485	30.10060590045112	21.310330508314614
39	21.85158270258027	26.382466008544263	29.900841213814278	21.865110075061185
40	21.19330300685177	26.330244058966763	31.367302767763327	21.109150166418143
41	20.073362401454663	27.550224302711136	30.851217778113337	21.525195517720856
42	22.01752895801481	27.341179209372267	30.593883110918163	20.047408721694758
43	22.690908976512706	25.648841993997625	30.902181367460063	20.758067662029607
44	21.12959852016837	26.10468298759886	30.37146794012722	22.39425055210555
45	21.26487224497757	26.425407551419745	29.50445774111755	22.805262462485135
46	22.068964432448077	26.379005517909608	30.179253414875074	21.372776634767234
47	19.80061282143239	26.43720467858334	32.14544127547393	21.616741224510342
48	20.27501462843768	25.997407777924586	31.78822426496033	21.9393533286774
49	21.226177667880986	24.846008166757898	32.01189779598206	21.91591636937906
50	19.79038864455728	25.109634635106993	31.460578719870135	23.639398000465594
51	19.31724519778277	24.904050032402775	30.42715038033938	25.351554389475073
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	420.0
1	791.5
2	1163.0
3	4098.0
4	7033.0
5	4598.5
6	2164.0
7	2126.5
8	2089.0
9	2167.5
10	2246.0
11	2334.0
12	2422.0
13	2490.0
14	2558.0
15	2539.5
16	2521.0
17	2628.0
18	2735.0
19	2933.0
20	3131.0
21	3207.0
22	3283.0
23	4086.5
24	4890.0
25	5672.5
26	7929.0
27	9403.0
28	10801.5
29	12200.0
30	14483.0
31	16766.0
32	18368.0
33	19970.0
34	22811.0
35	25652.0
36	27650.0
37	29648.0
38	30846.0
39	32044.0
40	34929.5
41	37815.0
42	41621.0
43	45427.0
44	47269.0
45	49111.0
46	53422.5
47	57734.0
48	60144.0
49	62554.0
50	58372.5
51	54191.0
52	45451.0
53	36711.0
54	31828.0
55	26945.0
56	24219.0
57	21493.0
58	19342.0
59	17191.0
60	15543.0
61	13895.0
62	12095.0
63	10295.0
64	8114.0
65	5933.0
66	4650.5
67	3368.0
68	2562.5
69	1757.0
70	1429.0
71	1101.0
72	885.0
73	669.0
74	545.5
75	300.0
76	178.0
77	134.0
78	90.0
79	68.5
80	47.0
81	30.5
82	14.0
83	13.5
84	13.0
85	7.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	635748.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.18695079401807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.19978115027375	24.292775351670265
2	9.516868719448256	6.31671707810918
3	3.403700307423734	3.388753038601669
4	1.8226356817771776	2.419508827462431
5	1.3081788580858928	2.170723369653563
6	1.0240634002225661	2.0391325003884684
7	0.8587447420434655	1.9949383649172758
8	0.744667125231161	1.9770584994375542
9	0.6477934643199477	1.9348460842565338
>10	7.33898635812862	47.48730161881405
>50	0.10188289262324389	2.2251326427038314
>100	0.02748468731143075	1.715753560765908
>500	0.003790991353300793	0.8498563008596671
>1k	0.0014216217574877976	1.1875027623596126
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4298	0.6760540339883098	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC	1833	0.2883217878782159	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	1420	0.22335894096402975	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	944	0.14848650723242543	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	854	0.13432995463611366	No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT	750	0.11797127163593123	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5729502884790828E-4	0.0	0.0	0.12080258215519357	0.0
2	1.5729502884790828E-4	0.0	0.0	0.6543473200072984	0.0
3	1.5729502884790828E-4	0.0	0.0	1.0057444144535257	0.0
4	1.5729502884790828E-4	0.0	0.0	1.4622145881701554	0.0
5	1.5729502884790828E-4	0.0	0.0	2.632332307769745	0.0
6	1.5729502884790828E-4	0.0	0.0	3.3621812416240395	0.0
7	1.5729502884790828E-4	0.0	0.0	4.097692796516859	0.0
8	1.5729502884790828E-4	0.0	0.0	5.132222201249552	0.0
9	1.5729502884790828E-4	0.0	0.0	5.584917294273832	0.0
10	1.5729502884790828E-4	0.0	0.0	6.5749322058425665	0.0
11	1.5729502884790828E-4	0.0	0.0	8.19365534771639	0.0
12	1.5729502884790828E-4	0.0	0.0	9.42024198267238	0.0
13	3.1459005769581656E-4	0.0	0.0	9.845725035705971	0.0
14	3.1459005769581656E-4	0.0	0.0	10.023153828246413	0.0
15	3.1459005769581656E-4	0.0	0.0	10.311161026066932	0.0
16	3.1459005769581656E-4	0.0	0.0	10.894568288063825	0.0
17	3.1459005769581656E-4	0.0	0.0	11.740343658179027	0.0
18	3.1459005769581656E-4	0.0	0.0	12.625442785506207	0.0
19	3.1459005769581656E-4	0.0	0.0	13.19894046068568	0.0
20	3.1459005769581656E-4	0.0	0.0	13.715182745364515	0.0
21	3.1459005769581656E-4	0.0	0.0	14.315420575448133	0.0
22	3.1459005769581656E-4	0.0	0.0	14.986913053599855	0.0
23	3.1459005769581656E-4	0.0	0.0	15.617036939164574	0.0
24	4.718850865437249E-4	0.0	0.0	16.082472929525537	0.0
25	4.718850865437249E-4	0.0	0.0	16.49128270950125	0.0
26	4.718850865437249E-4	0.0	0.0	16.886250526938348	0.0
27	4.718850865437249E-4	0.0	0.0	17.293330061596734	0.0
28	4.718850865437249E-4	0.0	0.0	17.68766869891844	0.0
29	4.718850865437249E-4	0.0	0.0	18.11551117738475	0.0
30	4.718850865437249E-4	0.0	0.0	18.634427477554	0.0
31	4.718850865437249E-4	0.0	0.0	19.10285207346307	0.0
32	4.718850865437249E-4	0.0	0.0	19.533211272390947	0.0
33	4.718850865437249E-4	0.0	0.0	19.951616049126383	0.0
34	4.718850865437249E-4	0.0	0.0	20.35790910864053	0.0
35	4.718850865437249E-4	0.0	0.0	20.84316427263633	0.0
36	4.718850865437249E-4	0.0	0.0	21.29947715132411	0.0
37	4.718850865437249E-4	0.0	0.0	21.764441256598527	0.0
38	6.291801153916331E-4	0.0	0.0	22.18143037807433	0.0
39	6.291801153916331E-4	0.0	0.0	22.629406620233173	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	30	2.1639644E-6	45.000004	1
GCGCGAC	30	2.1639644E-6	45.000004	9
TGCAACG	35	1.2107739E-7	45.000004	1
CGTATGG	55	1.8189894E-12	45.0	2
CGACGGT	40	6.8066583E-9	45.0	28
CGCATAC	25	3.888832E-5	45.0	26
GCGCTTA	20	7.030905E-4	45.0	1
TGTCGCG	25	3.888832E-5	45.0	1
TCGCGAG	25	3.888832E-5	45.0	1
AATCGCG	20	7.030905E-4	45.0	1
CGTTTTT	2340	0.0	42.980766	1
GCTACGA	105	0.0	42.857147	10
CGCACGG	70	0.0	41.785717	2
CGGGTCC	45	1.925946E-8	40.0	6
CGGGTAT	45	1.925946E-8	40.0	6
ACGATAG	45	1.925946E-8	40.0	1
ACGGTCT	45	1.925946E-8	40.0	30
GCGAGAC	40	3.4549703E-7	39.375	21
ACACGAC	40	3.4549703E-7	39.375	26
CACGACC	40	3.4549703E-7	39.375	27
>>END_MODULE