FastQCFastQC Report
Sat 18 Jun 2016
SRR3550566_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550566_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116258
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192401.7236158665828152No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC37980.34024392210402976No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG21460.19224946204192936No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC21020.18830772097490006TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTT17720.15874466297218026No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT16670.14933823542586033No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC14260.12774824458144982No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC14250.12765865955719913No Hit
GAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT12450.11153335519207926No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT12240.10965206968281527No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTC11520.1032019479367673No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA207.033198E-445.00000428
TAACGCC207.033198E-445.00000412
TATTGCG207.033198E-445.0000041
ACTACGC207.033198E-445.0000041
ACTCGTA253.8907332E-545.012
CGAATAT1850.043.78378314
CGTTTTT98300.043.718211
TTTGCGC2300.041.08695613
GTATACG556.184564E-1140.9090921
TACGAAT2100.040.71428712
CGTAAGG1400.040.178572
GACACGA850.039.70588325
CTACGAA2100.039.64285711
TCGCATG403.458099E-739.3750041
TAGCCGT1950.039.23076644
AACACGT659.094947E-1238.07692741
CTTGCGG1850.037.70272
CACGACC900.037.527
CGGTCTA900.037.531
GCCGATA601.5643309E-1037.4999969