##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550566_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1116258
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18412320449215	33.0	31.0	34.0	30.0	34.0
2	32.34183495213472	34.0	31.0	34.0	30.0	34.0
3	32.22931168242467	33.0	31.0	34.0	30.0	34.0
4	35.866113389556894	37.0	35.0	37.0	35.0	37.0
5	35.990465465869	37.0	35.0	37.0	35.0	37.0
6	36.03764004378916	37.0	35.0	37.0	35.0	37.0
7	36.136205070870716	37.0	36.0	37.0	35.0	37.0
8	36.1163369041924	37.0	37.0	37.0	35.0	37.0
9	37.94473589439001	39.0	38.0	39.0	35.0	39.0
10	37.576285231550415	39.0	37.0	39.0	35.0	39.0
11	37.52604953335161	39.0	37.0	39.0	35.0	39.0
12	37.47661203771888	39.0	37.0	39.0	35.0	39.0
13	37.46834602753127	39.0	37.0	39.0	35.0	39.0
14	38.77719039863544	40.0	38.0	41.0	35.0	41.0
15	38.866577439982514	40.0	38.0	41.0	35.0	41.0
16	38.90574670013563	40.0	38.0	41.0	35.0	41.0
17	38.84584119441921	40.0	38.0	41.0	35.0	41.0
18	38.64964730375952	40.0	38.0	41.0	35.0	41.0
19	38.427796262154445	40.0	37.0	41.0	35.0	41.0
20	38.20591117824016	40.0	36.0	41.0	34.0	41.0
21	38.14256023249106	40.0	35.0	41.0	34.0	41.0
22	38.15661164354477	40.0	35.0	41.0	34.0	41.0
23	38.116857393183295	40.0	35.0	41.0	34.0	41.0
24	38.00996633394789	40.0	35.0	41.0	34.0	41.0
25	37.95470491588862	40.0	35.0	41.0	34.0	41.0
26	37.97346401996671	40.0	35.0	41.0	34.0	41.0
27	37.894994705525065	40.0	35.0	41.0	34.0	41.0
28	37.81756995246619	40.0	35.0	41.0	34.0	41.0
29	37.730255908580276	40.0	35.0	41.0	34.0	41.0
30	37.55535996158594	40.0	35.0	41.0	33.0	41.0
31	37.403622639210646	40.0	35.0	41.0	33.0	41.0
32	37.058309100584275	40.0	35.0	41.0	32.0	41.0
33	36.71176645542518	40.0	35.0	41.0	31.0	41.0
34	36.45350537241391	40.0	35.0	41.0	30.0	41.0
35	36.2549822711237	40.0	35.0	41.0	29.0	41.0
36	36.14777228920196	40.0	35.0	41.0	28.0	41.0
37	36.07156320492216	40.0	35.0	41.0	28.0	41.0
38	35.96003343313105	40.0	35.0	41.0	27.0	41.0
39	35.85647314509728	39.0	35.0	41.0	26.0	41.0
40	35.74906518027194	39.0	35.0	41.0	25.0	41.0
41	35.64510265547929	39.0	35.0	41.0	25.0	41.0
42	35.58850910810941	39.0	35.0	41.0	24.0	41.0
43	35.481810656676146	39.0	35.0	41.0	24.0	41.0
44	35.41030209861878	39.0	35.0	41.0	23.0	41.0
45	35.31478027481102	39.0	35.0	41.0	23.0	41.0
46	35.18694871615702	39.0	35.0	41.0	23.0	41.0
47	35.09368443496037	39.0	35.0	41.0	23.0	41.0
48	35.01452352413152	38.0	35.0	41.0	23.0	41.0
49	34.926507133655484	38.0	35.0	41.0	23.0	41.0
50	34.81864945200841	38.0	35.0	41.0	23.0	41.0
51	33.889794294867315	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	39.0
10	37.0
11	32.0
12	27.0
13	35.0
14	38.0
15	52.0
16	89.0
17	164.0
18	344.0
19	629.0
20	1112.0
21	1954.0
22	3093.0
23	4859.0
24	7789.0
25	13403.0
26	19224.0
27	21214.0
28	19477.0
29	17629.0
30	17628.0
31	19284.0
32	22769.0
33	29665.0
34	55508.0
35	81233.0
36	71998.0
37	102028.0
38	189745.0
39	414631.0
40	514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.6639486570309	19.54548142096182	22.71150576300461	18.079064159002666
2	37.72147657620371	23.442250805817295	23.16704561131925	15.66922700665975
3	29.12722685974031	23.11230916150209	32.67909390123072	15.081370077526879
4	25.81938942430872	23.54796113443308	33.7456036149349	16.887045826323305
5	24.029391054756157	28.232182882451905	30.623117594677936	17.115308468114
6	23.829974790774173	32.9386217164849	29.889057905967974	13.34234558677295
7	73.96578568753819	5.848289553131982	16.04046734715451	4.145457412175321
8	75.56004077910305	5.966273030070109	14.618484257223688	3.8552019336031633
9	70.08146862105356	7.232199007756271	16.60646553037022	6.079866840819954
10	36.99512119957931	24.346163700506512	24.01093653976052	14.647778560153657
11	28.25260826798106	24.884390526204516	29.27369837439015	17.589302831424277
12	26.02668917042476	21.471917782448145	33.06386158038733	19.43753146673977
13	23.149307776517617	22.84337491870159	34.610099098953825	19.39721820582697
14	19.875512650301275	26.294906733031254	33.596265379509035	20.233315237158433
15	19.34651308210109	25.028264075151085	36.7443727167017	18.880850126046127
16	21.894490341838534	24.19915467571117	34.8554724803764	19.050882502073893
17	21.229679876874343	25.249091159928977	33.122360601223015	20.398868361973665
18	21.788063333028745	25.30848603100717	33.21006433996442	19.69338629599967
19	22.471686653085577	27.280969094958333	30.785624828668638	19.461719423287448
20	24.55534473213182	25.899657606037312	31.902391740977443	17.642605920853423
21	22.66447362527301	26.682182792866882	32.38839049753731	18.264953084322798
22	21.359219821940805	24.387641566734573	32.18413664224579	22.069001969078833
23	21.45937587905305	25.56971596172211	32.464358598101875	20.506549561122966
24	21.52692298733805	24.869519412178906	32.98144335807672	20.622114242406326
25	21.356890611310288	26.785474325827902	31.173259228601275	20.68437583426054
26	20.365721903000917	27.268875116684494	31.687298097751594	20.67810488256299
27	21.63550003672986	26.01334100181141	31.973611835256722	20.377547126202007
28	19.97996878857755	26.937141771884278	32.57866908904572	20.50422035049245
29	22.148911810710427	25.049674895946993	31.822571484370098	20.97884180897248
30	21.44710273073071	26.05374384774846	31.366673295958464	21.13248012556237
31	21.546094182527696	26.676628521363337	29.80556466336635	21.97171263274261
32	23.032399319870496	26.72124186344017	29.354235311191502	20.892123505497835
33	21.18524570484601	27.64764059921631	28.82057732173028	22.346536374207396
34	20.596672095519136	28.121545377502333	29.0386272707564	22.243155256222128
35	20.53557510898018	26.825608416692198	29.447582906460696	23.191233567866927
36	20.471073891519705	30.66665591646376	27.59783132573294	21.2644388662836
37	20.627489343861367	28.85506755606679	28.91876250830901	21.598680591762836
38	20.254367717857342	29.18070911921796	28.39809434736414	22.16682881556056
39	21.08715010329153	27.392860790247415	28.515540314156762	23.004448792304288
40	21.774804749439646	26.396496150531508	30.66289334544523	21.165805754583616
41	19.342571341034063	27.66896183498797	30.503879927400295	22.484586896577675
42	20.891227655255324	28.034020808809434	29.24565826179969	21.82909327413555
43	21.176018447348195	27.11675974550686	29.416496903045715	22.290724904099232
44	21.038684605171923	27.108069998154548	28.76790132747089	23.08534406920264
45	20.737320583592684	26.11027199805063	28.96427170062835	24.18813571772834
46	21.218033823721754	27.344395292127803	28.401946503406915	23.03562438074352
47	20.412843625756768	27.002359669538762	30.105853664654585	22.47894304004988
48	20.5513420732483	26.261401933961505	29.88180151900367	23.305454473786526
49	20.39967462719192	26.48957499072795	30.197947069584274	22.912803312495857
50	19.414060190386092	25.861673555755033	30.473868944276322	24.250397309582553
51	18.968553864787534	26.00680129504111	29.474637583784396	25.550007256386962
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1650.0
1	2025.5
2	2401.0
3	16450.5
4	30500.0
5	20232.5
6	9965.0
7	9756.0
8	9547.0
9	9681.5
10	9816.0
11	9767.0
12	9718.0
13	9460.0
14	9202.0
15	8772.0
16	8342.0
17	7831.0
18	7320.0
19	6946.0
20	6572.0
21	6666.0
22	6760.0
23	6953.5
24	7147.0
25	8063.0
26	9481.0
27	9983.0
28	11523.5
29	13064.0
30	14909.0
31	16754.0
32	19159.5
33	21565.0
34	24287.5
35	27010.0
36	29529.0
37	32048.0
38	34790.5
39	37533.0
40	41791.5
41	46050.0
42	51111.5
43	56173.0
44	62068.0
45	67963.0
46	81998.5
47	96034.0
48	95560.5
49	95087.0
50	91073.5
51	87060.0
52	80327.0
53	73594.0
54	68042.0
55	62490.0
56	57689.5
57	52889.0
58	50539.0
59	48189.0
60	45009.0
61	41829.0
62	37124.0
63	32419.0
64	28239.0
65	24059.0
66	19755.5
67	15452.0
68	13335.5
69	11219.0
70	9325.0
71	7431.0
72	6230.5
73	5030.0
74	4197.5
75	2675.0
76	1985.0
77	1542.0
78	1099.0
79	793.0
80	487.0
81	351.5
82	216.0
83	185.5
84	155.0
85	115.5
86	76.0
87	50.0
88	24.0
89	12.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	2.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1116258.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.843798409739616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.78495275022318	23.814369594600095
2	9.270876456550504	5.904398419279907
3	3.517518226028544	3.3603342387671353
4	1.864986594391381	2.3755302859466383
5	1.2664439027845242	2.016419216875713
6	0.9022313975808983	1.7238284846102254
7	0.7284730999208524	1.6238145378558408
8	0.5996604913189063	1.527637423987573
9	0.5174860567196673	1.4830849503028947
>10	6.180419353634208	43.2113487237461
>50	0.31934768122724444	6.212613080233246
>100	0.039386634270173054	2.176116888108213
>500	0.004533713296566046	1.019888426583293
>1k	0.0034002849724245345	1.8145586356356738
>5k	0.0	0.0
>10k+	2.833570810353779E-4	1.7360570934674473
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19240	1.7236158665828152	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	3798	0.34024392210402976	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	2146	0.19224946204192936	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	2102	0.18830772097490006	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTT	1772	0.15874466297218026	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT	1667	0.14933823542586033	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC	1426	0.12774824458144982	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	1425	0.12765865955719913	No Hit
GAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	1245	0.11153335519207926	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1224	0.10965206968281527	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTC	1152	0.1032019479367673	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5834009700266427E-4	0.0	0.0	0.10463530832477796	0.0
2	3.5834009700266427E-4	0.0	0.0	0.4707693024372502	0.0
3	3.5834009700266427E-4	0.0	0.0	0.69132763214239	0.0
4	3.5834009700266427E-4	0.0	0.0	1.1028812335499498	0.0
5	3.5834009700266427E-4	0.0	0.0	2.1554156834710256	0.0
6	3.5834009700266427E-4	0.0	0.0	3.0063838288281026	0.0
7	3.5834009700266427E-4	0.0	0.0	3.7170618262086363	0.0
8	3.5834009700266427E-4	0.0	0.0	4.661377566834908	0.0
9	3.5834009700266427E-4	0.0	0.0	5.167174613754168	0.0
10	3.5834009700266427E-4	0.0	0.0	6.163180913373073	0.0
11	3.5834009700266427E-4	0.0	0.0	7.121292747733947	0.0
12	4.479251212533303E-4	0.0	0.0	7.993761298911184	0.0
13	4.479251212533303E-4	0.0	0.0	8.358282762587145	0.0
14	4.479251212533303E-4	0.0	0.0	8.536467375821719	0.0
15	4.479251212533303E-4	0.0	0.0	8.78004905675928	0.0
16	4.479251212533303E-4	0.0	0.0	9.194558963967111	0.0
17	4.479251212533303E-4	0.0	0.0	9.68781410749128	0.0
18	4.479251212533303E-4	0.0	0.0	10.232849395032332	0.0
19	4.479251212533303E-4	0.0	0.0	10.627650596905017	0.0
20	4.479251212533303E-4	0.0	0.0	10.984736503568172	0.0
21	4.479251212533303E-4	0.0	0.0	11.439470086664553	0.0
22	4.479251212533303E-4	0.0	0.0	11.943654603147301	0.0
23	4.479251212533303E-4	0.0	0.0	12.456439281958113	0.0
24	4.479251212533303E-4	0.0	0.0	12.866649108001914	0.0
25	4.479251212533303E-4	0.0	0.0	13.245145835460978	0.0
26	5.375101455039964E-4	0.0	0.0	13.59515452520833	0.0
27	6.270951697546624E-4	0.0	0.0	13.950717486459224	0.0
28	6.270951697546624E-4	0.0	0.0	14.32016612646897	0.0
29	6.270951697546624E-4	0.0	0.0	14.726882136566994	0.0
30	6.270951697546624E-4	0.0	0.0	15.16477373510425	0.0
31	7.166801940053285E-4	0.0	0.0	15.585196253912626	0.0
32	7.166801940053285E-4	0.0	0.0	15.994331059665418	0.0
33	7.166801940053285E-4	0.0	0.0	16.40866179682475	0.0
34	7.166801940053285E-4	0.0	0.0	16.791189850375094	0.0
35	7.166801940053285E-4	0.0	0.0	17.18778275273279	0.0
36	7.166801940053285E-4	0.0	0.0	17.590288266691033	0.0
37	7.166801940053285E-4	0.0	0.0	18.011964975838918	0.0
38	7.166801940053285E-4	0.0	0.0	18.42790824343476	0.0
39	7.166801940053285E-4	0.0	0.0	18.830413757393003	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCGA	20	7.033198E-4	45.000004	28
TAACGCC	20	7.033198E-4	45.000004	12
TATTGCG	20	7.033198E-4	45.000004	1
ACTACGC	20	7.033198E-4	45.000004	1
ACTCGTA	25	3.8907332E-5	45.0	12
CGAATAT	185	0.0	43.783783	14
CGTTTTT	9830	0.0	43.71821	1
TTTGCGC	230	0.0	41.086956	13
GTATACG	55	6.184564E-11	40.909092	1
TACGAAT	210	0.0	40.714287	12
CGTAAGG	140	0.0	40.17857	2
GACACGA	85	0.0	39.705883	25
CTACGAA	210	0.0	39.642857	11
TCGCATG	40	3.458099E-7	39.375004	1
TAGCCGT	195	0.0	39.230766	44
AACACGT	65	9.094947E-12	38.076927	41
CTTGCGG	185	0.0	37.7027	2
CACGACC	90	0.0	37.5	27
CGGTCTA	90	0.0	37.5	31
GCCGATA	60	1.5643309E-10	37.499996	9
>>END_MODULE