FastQCFastQC Report
Sat 18 Jun 2016
SRR3550563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784331
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87541.1161104176680507No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT20830.2655766506742689No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCT14080.17951604615908334No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC13940.17773108547284247No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG9510.12124982947250586No Hit
CGTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT9420.12010235474563671No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT8610.10977508220381446No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC8440.10760762994195053No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCC8070.10289023384259961No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG253.889668E-545.01
TAGCCGT453.8380676E-1045.044
TATTGCG551.8189894E-1245.01
ACGTACG207.031915E-445.01
CGTTTTT46900.042.601281
TCTAGCG403.4563527E-739.3751
GTATGCG403.4563527E-739.3751
GTACGAG750.039.0000041
GGCGATA2600.038.9423078
TCACGAC356.245751E-638.57142625
GTATACG356.245751E-638.5714261
AATAGCG659.094947E-1238.076921
ACGTTCG301.13959104E-437.50000413
GCGGTAT301.13959104E-437.5000045
TCGATGG601.546141E-1037.5000042
GCTTGCG601.546141E-1037.5000041
CGGTCTA301.13959104E-437.50000431
CGCCGGT601.546141E-1037.50000428
TTCGAAT301.13959104E-437.50000416
TATGCGG1150.037.1739162