##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550563_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	784331
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21039969094681	33.0	31.0	34.0	30.0	34.0
2	32.24730502810676	34.0	31.0	34.0	30.0	34.0
3	32.184251291865294	33.0	31.0	34.0	30.0	34.0
4	35.866278395218345	37.0	35.0	37.0	35.0	37.0
5	35.95056551379456	37.0	35.0	37.0	35.0	37.0
6	35.914162515570595	37.0	35.0	37.0	35.0	37.0
7	36.19084162171328	37.0	36.0	37.0	35.0	37.0
8	36.04930826398549	37.0	36.0	37.0	35.0	37.0
9	37.92105883867908	39.0	38.0	39.0	35.0	39.0
10	37.523188551772144	39.0	37.0	39.0	35.0	39.0
11	37.50908226246317	39.0	37.0	39.0	35.0	39.0
12	37.54186306546598	39.0	37.0	39.0	35.0	39.0
13	37.57301572932856	39.0	37.0	39.0	35.0	39.0
14	38.813242113342454	40.0	38.0	41.0	35.0	41.0
15	38.929608800366175	40.0	38.0	41.0	35.0	41.0
16	38.69099908074524	40.0	38.0	41.0	35.0	41.0
17	38.73763500358905	40.0	38.0	41.0	35.0	41.0
18	38.57493201212243	40.0	38.0	41.0	35.0	41.0
19	38.36602148837672	40.0	37.0	41.0	34.0	41.0
20	38.46194272571147	40.0	37.0	41.0	35.0	41.0
21	38.37533388327122	40.0	37.0	41.0	34.0	41.0
22	38.47769755371138	40.0	37.0	41.0	35.0	41.0
23	38.48299506203376	40.0	37.0	41.0	35.0	41.0
24	38.42186015853001	40.0	37.0	41.0	35.0	41.0
25	38.172749770186314	40.0	37.0	41.0	34.0	41.0
26	38.27024049795303	40.0	37.0	41.0	34.0	41.0
27	38.28403059422616	40.0	37.0	41.0	34.0	41.0
28	38.18651819193682	40.0	37.0	41.0	34.0	41.0
29	38.147113909816134	40.0	37.0	41.0	34.0	41.0
30	37.94773507613495	40.0	36.0	41.0	34.0	41.0
31	37.86306801592695	40.0	36.0	41.0	34.0	41.0
32	37.70664298618823	40.0	36.0	41.0	33.0	41.0
33	37.52573467069388	40.0	36.0	41.0	33.0	41.0
34	37.354119625515246	40.0	36.0	41.0	33.0	41.0
35	37.206579875078255	40.0	36.0	41.0	32.0	41.0
36	37.05573412245595	40.0	36.0	41.0	32.0	41.0
37	37.027573817686665	40.0	36.0	41.0	32.0	41.0
38	36.887829245560866	40.0	36.0	41.0	31.0	41.0
39	36.8311554688008	40.0	35.0	41.0	31.0	41.0
40	36.75428996176359	40.0	35.0	41.0	31.0	41.0
41	36.62043703487431	40.0	35.0	41.0	30.0	41.0
42	36.605011149629426	40.0	35.0	41.0	30.0	41.0
43	36.523725314949935	40.0	35.0	41.0	30.0	41.0
44	36.399601698772585	39.0	35.0	41.0	30.0	41.0
45	36.36181280607295	39.0	35.0	41.0	30.0	41.0
46	36.220641540370075	39.0	35.0	41.0	29.0	41.0
47	36.172757420017824	39.0	35.0	41.0	29.0	41.0
48	36.0439699565617	39.0	35.0	41.0	29.0	41.0
49	35.95957064045665	39.0	35.0	41.0	29.0	41.0
50	35.83113379427818	39.0	35.0	41.0	28.0	41.0
51	34.81806915702682	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	12.0
10	16.0
11	6.0
12	11.0
13	13.0
14	10.0
15	34.0
16	45.0
17	103.0
18	204.0
19	336.0
20	584.0
21	903.0
22	1354.0
23	2161.0
24	3451.0
25	5740.0
26	8773.0
27	10599.0
28	10306.0
29	10577.0
30	10729.0
31	12630.0
32	15223.0
33	20145.0
34	35413.0
35	49183.0
36	50812.0
37	77177.0
38	152054.0
39	305527.0
40	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.99463364319401	20.277535887272084	24.576613700083254	16.151216769450652
2	35.58229879986893	23.771978922164237	24.59956319462064	16.04615908334619
3	30.15244839232416	23.231135834233253	30.998264763218593	15.618151010223999
4	27.457795242059795	24.9109113371778	30.034513489840386	17.596779930922022
5	24.20916679310138	29.480283196762592	28.24190297208704	18.068647038048987
6	25.640832760658444	33.05237202150623	26.944109055998037	14.362686161837285
7	79.32033796955622	5.119139750946985	11.59153469644831	3.9689875830484835
8	81.56416104935289	4.255091281614522	10.684009684686695	3.496737984345895
9	74.72329921933469	7.722249917445569	12.746659254829911	4.8077916083898256
10	33.46418795126037	35.806438863184034	18.523812013040413	12.205561172515175
11	24.074402261290196	24.237981158464986	33.63834911536073	18.049267464884085
12	23.655446488791085	22.00805527258262	35.37294841081125	18.963549827815044
13	22.002317898948277	22.4530204722241	36.10771472758312	19.4369469012445
14	18.82802031285261	26.55384015167066	34.635632150201886	19.98250738527484
15	18.481992934105627	26.53497056727326	36.361816630988706	18.621219867632416
16	21.684722393989272	25.369008747582335	34.456881087194056	18.489387771234338
17	21.190033289516798	24.971217508934366	32.553985498469395	21.28476370307944
18	21.386761456578917	25.782864632406472	32.47748718334479	20.352886727669823
19	22.175331588321768	26.781422639166376	31.400900895157786	19.642344877354077
20	23.634154457748068	26.36068190598102	31.251856677856672	18.75330695841424
21	23.29819935715916	25.98902759166729	31.590361722283067	19.122411328890482
22	21.948004095209804	24.332711572027627	31.4853040361786	22.23398029658397
23	20.70312151374866	26.424940490685696	31.951816261246847	20.9201217343188
24	21.157139014013215	25.637645330861584	32.49814172842843	20.707073926696765
25	21.830324187109778	26.705306815617387	30.197964889823304	21.26640410744953
26	20.709751367726128	27.666890636733726	30.76864232065289	20.854715674887263
27	21.906822502234387	26.072920743920612	31.458912117460613	20.561344636384383
28	21.167593783746913	26.705561810001132	31.867158125842277	20.259686280409674
29	22.38034707285572	25.457109307167507	31.069280699092605	21.093262920884168
30	23.896671175817353	24.92557351424335	30.47552627653376	20.70222903340554
31	24.184942326645256	26.107982471686057	28.987506550168234	20.71956865150045
32	23.92446556364596	27.32481567093485	28.807353018049774	19.943365747369416
33	23.049452335812305	28.03523002405872	28.64058669107813	20.27473094905085
34	22.35395515413773	27.499996812570203	30.06485782150648	20.081190211785586
35	22.338655491112807	27.468760000560987	29.451596328590863	20.740988179735343
36	22.70775986158905	28.58729286487465	29.13170587417812	19.573241399358178
37	22.349110260846505	28.434933720584805	30.044330773614714	19.17162524495398
38	20.687821850723736	29.086699365446478	29.621040096591873	20.604438687237913
39	21.513111173726397	26.95851623867984	29.998686778923695	21.529685808670067
40	21.763388161375747	26.697656984104924	31.53158551682899	20.007369337690335
41	20.286715685087035	27.13280489997208	31.46350201636809	21.116977398572796
42	21.76695808274823	27.743516449050208	29.840590260999498	20.64893520720206
43	22.36096749969082	26.263529045772767	30.223591825390045	21.15191162914637
44	22.36568489579017	26.62600356227154	29.83090047441705	21.177411067521238
45	22.18744382154983	25.735180682645463	29.65635681874107	22.421018677063635
46	22.4286685085761	26.73973105742346	28.96608702193334	21.8655134120671
47	21.782002751389403	26.375216585854695	30.46124659104383	21.38153407171207
48	21.34098996469603	25.67525700246452	30.594991145320023	22.388761887519426
49	20.845535877072308	26.24682691363723	30.720830873700006	22.186806335590457
50	19.95229055080062	25.729698303394866	31.518580803257805	22.799430342546707
51	20.21442477729428	25.25043635913919	30.5558495074146	23.97928935615193
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	432.0
1	741.0
2	1050.0
3	7902.5
4	14755.0
5	9824.0
6	4893.0
7	4626.5
8	4360.0
9	4392.0
10	4424.0
11	4313.5
12	4203.0
13	3961.0
14	3719.0
15	3592.0
16	3465.0
17	3302.5
18	3140.0
19	3285.0
20	3430.0
21	3429.0
22	3428.0
23	3885.0
24	4342.0
25	5231.0
26	7333.0
27	8546.0
28	10083.5
29	11621.0
30	14108.0
31	16595.0
32	18692.5
33	20790.0
34	23627.5
35	26465.0
36	27347.0
37	28229.0
38	31700.5
39	35172.0
40	37896.5
41	40621.0
42	44901.5
43	49182.0
44	56677.0
45	64172.0
46	66663.0
47	69154.0
48	67125.0
49	65096.0
50	58529.5
51	51963.0
52	47877.0
53	43791.0
54	40112.5
55	36434.0
56	34467.0
57	32500.0
58	31288.0
59	30076.0
60	28920.0
61	27764.0
62	24834.0
63	21904.0
64	18424.0
65	14944.0
66	12262.0
67	9580.0
68	8047.0
69	6514.0
70	5499.5
71	4485.0
72	3709.0
73	2933.0
74	2465.5
75	1496.0
76	994.0
77	730.0
78	466.0
79	379.0
80	292.0
81	216.5
82	141.0
83	117.5
84	94.0
85	64.0
86	34.0
87	22.5
88	11.0
89	7.5
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	784331.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.82719375409262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.67899995289633	22.495142258463346
2	8.435150284549573	5.369343249024975
3	3.449096251033147	3.293251639744394
4	2.2474696571875983	2.8612260894301524
5	1.756353102574956	2.7949895248127414
6	1.4670420043862475	2.801509807139609
7	1.261211769145214	2.8098581939068663
8	1.1532668165280486	2.936419713584303
9	1.0163476024413536	2.9112742857967615
>10	8.44394774975793	46.32541900613367
>50	0.0584586172901936	1.2468410290507084
>100	0.027818237807984616	1.5563378111160546
>500	0.0032253029342590813	0.8482932862798439
>1k	0.0012094886003471556	0.6268208597000511
>5k	4.0316286678238517E-4	1.123273245816632
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8754	1.1161104176680507	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT	2083	0.2655766506742689	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCT	1408	0.17951604615908334	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC	1394	0.17773108547284247	No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	951	0.12124982947250586	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT	942	0.12010235474563671	No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	861	0.10977508220381446	No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	844	0.10760762994195053	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCC	807	0.10289023384259961	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.374859593717448E-4	0.0	0.0	0.11512996426253712	0.0
2	6.374859593717448E-4	0.0	0.0	0.38861144083301563	0.0
3	6.374859593717448E-4	0.0	0.0	0.6666828163109707	0.0
4	6.374859593717448E-4	0.0	0.0	1.0173000939654304	0.0
5	6.374859593717448E-4	0.0	0.0	1.9216376759301876	0.0
6	6.374859593717448E-4	0.0	0.0	2.9201956826900886	0.0
7	6.374859593717448E-4	0.0	0.0	3.6934661514080154	0.0
8	6.374859593717448E-4	0.0	0.0	4.7486329113601276	0.0
9	6.374859593717448E-4	0.0	0.0	5.314720443282237	0.0
10	6.374859593717448E-4	0.0	0.0	6.142049721354887	0.0
11	6.374859593717448E-4	0.0	0.0	7.01897540706666	0.0
12	6.374859593717448E-4	0.0	0.0	7.707587740380018	0.0
13	6.374859593717448E-4	0.0	0.0	8.048260237068279	0.0
14	6.374859593717448E-4	0.0	0.0	8.214771569656179	0.0
15	6.374859593717448E-4	0.0	0.0	8.415962138433901	0.0
16	6.374859593717448E-4	0.0	0.0	8.769129359925847	0.0
17	7.649831512460938E-4	0.0	0.0	9.231944166429734	0.0
18	7.649831512460938E-4	0.0	0.0	9.74983775982334	0.0
19	7.649831512460938E-4	0.0	0.0	10.073298135608564	0.0
20	7.649831512460938E-4	0.0	0.0	10.381713842752614	0.0
21	7.649831512460938E-4	0.0	0.0	10.731183645680204	0.0
22	7.649831512460938E-4	0.0	0.0	11.154856814278666	0.0
23	8.924803431204427E-4	0.0	0.0	11.556217974299116	0.0
24	8.924803431204427E-4	0.0	0.0	11.898930426057365	0.0
25	8.924803431204427E-4	0.0	0.0	12.180061734140306	0.0
26	8.924803431204427E-4	0.0	0.0	12.454180696670155	0.0
27	8.924803431204427E-4	0.0	0.0	12.740029400852446	0.0
28	8.924803431204427E-4	0.0	0.0	13.033782930930947	0.0
29	8.924803431204427E-4	0.0	0.0	13.351633430273699	0.0
30	0.0010199775349947918	0.0	0.0	13.733615017129248	0.0
31	0.0011474747268691407	0.0	0.0	14.070335100869404	0.0
32	0.0011474747268691407	0.0	0.0	14.394560459805874	0.0
33	0.0012749719187434897	0.0	0.0	14.711773473189252	0.0
34	0.0012749719187434897	0.0	0.0	15.046708596243167	0.0
35	0.0012749719187434897	0.0	0.0	15.38597862382081	0.0
36	0.0012749719187434897	0.0	0.0	15.696051794459226	0.0
37	0.0012749719187434897	0.0	0.0	16.011607344348242	0.0
38	0.0012749719187434897	0.0	0.0	16.336215194860333	0.0
39	0.0012749719187434897	0.0	0.0	16.668090385309263	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	25	3.889668E-5	45.0	1
TAGCCGT	45	3.8380676E-10	45.0	44
TATTGCG	55	1.8189894E-12	45.0	1
ACGTACG	20	7.031915E-4	45.0	1
CGTTTTT	4690	0.0	42.60128	1
TCTAGCG	40	3.4563527E-7	39.375	1
GTATGCG	40	3.4563527E-7	39.375	1
GTACGAG	75	0.0	39.000004	1
GGCGATA	260	0.0	38.942307	8
TCACGAC	35	6.245751E-6	38.571426	25
GTATACG	35	6.245751E-6	38.571426	1
AATAGCG	65	9.094947E-12	38.07692	1
ACGTTCG	30	1.13959104E-4	37.500004	13
GCGGTAT	30	1.13959104E-4	37.500004	5
TCGATGG	60	1.546141E-10	37.500004	2
GCTTGCG	60	1.546141E-10	37.500004	1
CGGTCTA	30	1.13959104E-4	37.500004	31
CGCCGGT	60	1.546141E-10	37.500004	28
TTCGAAT	30	1.13959104E-4	37.500004	16
TATGCGG	115	0.0	37.173916	2
>>END_MODULE