##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550560_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595269
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.213782340420885	33.0	31.0	34.0	30.0	34.0
2	32.233598591561126	34.0	31.0	34.0	30.0	34.0
3	32.22484624598291	34.0	31.0	34.0	30.0	34.0
4	35.87828695934107	37.0	35.0	37.0	35.0	37.0
5	35.94755648286741	37.0	35.0	37.0	35.0	37.0
6	35.88453959470424	37.0	35.0	37.0	35.0	37.0
7	36.15342307427398	37.0	35.0	37.0	35.0	37.0
8	35.97590669092461	37.0	35.0	37.0	35.0	37.0
9	37.83055391764059	39.0	38.0	39.0	35.0	39.0
10	37.46409774404513	39.0	37.0	39.0	35.0	39.0
11	37.426975031456365	39.0	37.0	39.0	35.0	39.0
12	37.30605994936743	39.0	37.0	39.0	35.0	39.0
13	37.28108132625754	39.0	37.0	39.0	34.0	39.0
14	38.437126744379434	40.0	38.0	41.0	34.0	41.0
15	38.55579242325739	40.0	38.0	41.0	35.0	41.0
16	38.330144858878924	40.0	37.0	41.0	34.0	41.0
17	38.379661967950625	40.0	37.0	41.0	34.0	41.0
18	38.28324673382958	40.0	37.0	41.0	34.0	41.0
19	38.127364267247245	40.0	37.0	41.0	34.0	41.0
20	38.27496476382946	40.0	37.0	41.0	34.0	41.0
21	38.16182935781974	40.0	37.0	41.0	34.0	41.0
22	38.24628361295481	40.0	37.0	41.0	34.0	41.0
23	38.252344738261186	40.0	37.0	41.0	34.0	41.0
24	38.17476804604305	40.0	36.0	41.0	34.0	41.0
25	37.89945722018113	40.0	36.0	41.0	34.0	41.0
26	37.99161723523315	40.0	36.0	41.0	34.0	41.0
27	37.99694087882957	40.0	36.0	41.0	34.0	41.0
28	37.89266533281592	40.0	36.0	41.0	34.0	41.0
29	37.86950941507117	40.0	36.0	41.0	34.0	41.0
30	37.68653667501583	40.0	36.0	41.0	33.0	41.0
31	37.63616785016522	40.0	35.0	41.0	33.0	41.0
32	37.565603113886326	40.0	35.0	41.0	33.0	41.0
33	37.47448296484446	40.0	35.0	41.0	33.0	41.0
34	37.35647917160141	40.0	35.0	41.0	33.0	41.0
35	37.24642136580269	40.0	35.0	41.0	33.0	41.0
36	37.11129926134235	40.0	35.0	41.0	32.0	41.0
37	37.05887758307588	40.0	35.0	41.0	32.0	41.0
38	36.936338025329725	39.0	35.0	41.0	32.0	41.0
39	36.85381398997764	39.0	35.0	41.0	32.0	41.0
40	36.76675251020967	39.0	35.0	41.0	31.0	41.0
41	36.61854556511426	39.0	35.0	41.0	31.0	41.0
42	36.58648946946675	39.0	35.0	41.0	31.0	41.0
43	36.498112617992874	39.0	35.0	41.0	31.0	41.0
44	36.38808673053695	39.0	35.0	41.0	31.0	41.0
45	36.36656032818776	39.0	35.0	41.0	31.0	41.0
46	36.2339832915875	38.0	35.0	41.0	31.0	41.0
47	36.16320184656013	38.0	35.0	41.0	31.0	41.0
48	36.03674977195184	38.0	35.0	40.0	30.0	41.0
49	35.95961321688178	38.0	35.0	40.0	30.0	41.0
50	35.85520663767137	38.0	35.0	40.0	30.0	41.0
51	34.79306330415325	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	13.0
10	16.0
11	14.0
12	17.0
13	14.0
14	26.0
15	30.0
16	55.0
17	101.0
18	187.0
19	285.0
20	499.0
21	719.0
22	1095.0
23	1605.0
24	2456.0
25	3413.0
26	4775.0
27	5832.0
28	6205.0
29	7005.0
30	8029.0
31	9762.0
32	12771.0
33	18335.0
34	36728.0
35	49377.0
36	43539.0
37	66542.0
38	114990.0
39	200688.0
40	137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.90105649714667	20.643104210029414	24.740243486558178	12.715595806265739
2	33.46587845159079	24.54352570014565	24.720588507044713	17.270007341218843
3	32.63667350391168	23.72171236869382	26.98813477604243	16.653479351352075
4	29.52009931644349	25.561888826732115	25.82664308069125	19.091368776133145
5	25.72853617440182	30.502344318282994	24.55427714193079	19.21484236538439
6	26.86214131762279	34.97947986540539	23.541457727514786	14.616921089457035
7	80.76751855043686	6.40550742605444	8.583178361379478	4.243795662129222
8	82.46523840482202	5.416710764377113	8.059213565631671	4.058837265169193
9	76.3367485960129	7.625292094834436	10.203790219211818	5.8341690899408505
10	42.9931677947281	24.939313150861206	17.20432275156274	14.863196302847955
11	33.08705811994241	24.14538637153959	22.846309819594165	19.92124568892383
12	29.505820057822596	22.72468413439974	27.08422578699714	20.68527002078052
13	25.935165446210036	23.12467136706262	29.02754888966165	21.912614297065698
14	21.66852297028738	26.58008396204069	29.329933189868783	22.421459877803144
15	21.72480004838149	24.475657223876937	32.26322889315586	21.53631383458571
16	24.906218869116316	23.367418763617792	30.378030772642283	21.348331594623605
17	24.007801514945342	23.772277743339565	28.269740235086992	23.950180506628097
18	24.907226816783673	24.281627297910692	28.246389447459887	22.564756437845748
19	26.61637007806555	26.08383772714521	25.45319846993544	21.846593724853804
20	28.414884699186416	24.9465367758106	25.807996048845144	20.83058247615784
21	27.18686845778967	25.22288242794434	26.318857524917306	21.27139158934868
22	25.507627643972725	23.77076582183853	26.593859246827904	24.12774728736084
23	24.919154197514064	25.114024079869772	26.948992808293394	23.01782891432277
24	24.99491826384374	23.928509631779917	28.066302797558752	23.01026930681759
25	25.273111820034305	24.924529918406638	26.12885938962049	23.67349887193857
26	23.60915821250561	26.086861570147278	26.410916745202588	23.893063472144526
27	24.151770040099517	25.561552844176333	27.245497413774277	23.041179701949872
28	22.971127339068556	26.72623637380747	27.4494388251362	22.853197461987772
29	24.323121143550228	25.997154227752496	26.45459447745473	23.22513015124255
30	24.282299263022264	25.46445388555426	26.56597269469769	23.68727415672578
31	25.067490495893452	27.00308599977489	24.381078134423262	23.548345369908393
32	25.73122403484811	27.136807056977602	24.033168197907166	23.098800710267124
33	24.42878765734483	27.098001071784356	24.457178183308724	24.016033087562093
34	22.49554403135389	27.82792317422879	25.772045915376076	23.90448687904124
35	23.18044447132305	26.965623944804783	25.721816523286112	24.132115060586052
36	23.44906252467372	29.544122069182166	23.84837779222503	23.158437613919087
37	24.079029816771914	29.27617598094307	24.972743415161887	21.672050787123133
38	24.064918549428914	28.478217410952023	24.25390873705837	23.20295530256069
39	23.571528166257607	25.712241020446218	26.3448961729907	24.371334640305477
40	24.298258434422085	24.833646637066604	27.871936889036718	22.99615803947459
41	22.07489387151019	25.895015530793643	27.246673352719526	24.78341724497664
42	23.157093683695944	26.44804281761691	26.771426027560647	23.6234374711265
43	23.164149317367443	25.57448817257408	27.04659574074914	24.214766769309335
44	23.406560731366827	25.40061719995498	26.323057306864627	24.869764761813567
45	23.338524263820222	24.96434385126724	26.447034869949555	25.250097014962986
46	23.093592980652446	26.327089097534056	25.726184296511324	24.853133625302174
47	22.982886728521056	26.259892586376914	27.067090676652068	23.690130008449962
48	22.58558735630446	25.720808575618754	27.099345002007496	24.59425906606929
49	22.397941098898144	25.722152505841894	27.26212855028567	24.617777844974288
50	21.010165152225298	25.44311899326187	28.101244983360463	25.44547087115237
51	21.46626147170439	25.517203146812616	26.622417764069688	26.394117617413304
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	308.0
1	638.0
2	968.0
3	3118.0
4	5268.0
5	3536.0
6	1804.0
7	1769.5
8	1735.0
9	1702.5
10	1670.0
11	1688.0
12	1706.0
13	1657.0
14	1608.0
15	1540.0
16	1472.0
17	1424.5
18	1377.0
19	1395.0
20	1413.0
21	1489.5
22	1566.0
23	1808.0
24	2050.0
25	2463.0
26	3527.5
27	4179.0
28	5119.0
29	6059.0
30	6913.5
31	7768.0
32	9019.5
33	10271.0
34	11553.0
35	12835.0
36	13706.5
37	14578.0
38	15574.0
39	16570.0
40	19768.5
41	22967.0
42	25711.5
43	28456.0
44	33918.0
45	39380.0
46	43698.0
47	48016.0
48	48224.5
49	48433.0
50	44829.5
51	41226.0
52	38700.0
53	36174.0
54	34805.0
55	33436.0
56	33470.5
57	33505.0
58	33292.0
59	33079.0
60	33087.5
61	33096.0
62	30728.0
63	28360.0
64	25091.5
65	21823.0
66	18299.5
67	14776.0
68	13392.0
69	12008.0
70	10066.5
71	8125.0
72	7260.5
73	6396.0
74	4929.5
75	2830.0
76	2197.0
77	1688.0
78	1179.0
79	933.0
80	687.0
81	482.5
82	278.0
83	166.0
84	54.0
85	50.0
86	46.0
87	27.5
88	9.0
89	9.5
90	10.0
91	9.0
92	8.0
93	4.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	595269.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.71299330036609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.96354849991458	21.085394411481666
2	8.055411688393102	4.787007870578299
3	3.2011697996797066	2.853490104336522
4	1.9754501173518217	2.347861444083284
5	1.48396065438929	2.204645649093793
6	1.2715871678989765	2.2669596600368274
7	1.1077391502164409	2.3039972164254037
8	1.0051320730736846	2.3892386042577174
9	0.8905351646894871	2.3814418843943232
>10	9.930057667886192	52.73291755131333
>50	0.07390661155599917	1.5355014373082212
>100	0.03809033057116848	2.0873697756674994
>500	0.0028425619829230207	0.5062593847758756
>1k	5.685123965846041E-4	0.5179150062471921
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3066	0.5150612580194837	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	728	0.12229765030599611	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.039738336785555E-4	0.0	0.0	0.08113978722224742	0.0
2	5.039738336785555E-4	0.0	0.0	0.3171675326617042	0.0
3	5.039738336785555E-4	0.0	0.0	0.500949990676484	0.0
4	5.039738336785555E-4	0.0	0.0	0.7631843754672257	0.0
5	5.039738336785555E-4	0.0	0.0	1.471435603063489	0.0
6	5.039738336785555E-4	0.0	0.0	2.141552810578075	0.0
7	5.039738336785555E-4	0.0	0.0	2.7189388326958066	0.0
8	5.039738336785555E-4	0.0	0.0	3.627267672262456	0.0
9	5.039738336785555E-4	0.0	0.0	4.102682988699227	0.0
10	5.039738336785555E-4	0.0	0.0	4.868891207168524	0.0
11	5.039738336785555E-4	0.0	0.0	5.735054235984068	0.0
12	5.039738336785555E-4	0.0	0.0	6.374765022200047	0.0
13	5.039738336785555E-4	0.0	0.0	6.663206046342074	0.0
14	5.039738336785555E-4	0.0	0.0	6.79373526926482	0.0
15	5.039738336785555E-4	0.0	0.0	6.991628994622599	0.0
16	5.039738336785555E-4	0.0	0.0	7.363561683877373	0.0
17	5.039738336785555E-4	0.0	0.0	7.799499050009324	0.0
18	5.039738336785555E-4	0.0	0.0	8.272058514721916	0.0
19	5.039738336785555E-4	0.0	0.0	8.563019408032336	0.0
20	5.039738336785555E-4	0.0	0.0	8.86725161229629	0.0
21	5.039738336785555E-4	0.0	0.0	9.243048100942598	0.0
22	5.039738336785555E-4	0.0	0.0	9.658154548615835	0.0
23	5.039738336785555E-4	0.0	0.0	10.055285929554538	0.0
24	5.039738336785555E-4	0.0	0.0	10.394628310898097	0.0
25	5.039738336785555E-4	0.0	0.0	10.703228288387267	0.0
26	5.039738336785555E-4	0.0	0.0	10.967142585956937	0.0
27	5.039738336785555E-4	0.0	0.0	11.247520028760107	0.0
28	5.039738336785555E-4	0.0	0.0	11.527057515173812	0.0
29	5.039738336785555E-4	0.0	0.0	11.831625701993552	0.0
30	5.039738336785555E-4	0.0	0.0	12.204062365082004	0.0
31	5.039738336785555E-4	0.0	0.0	12.524925705857353	0.0
32	5.039738336785555E-4	0.0	0.0	12.873675598762912	0.0
33	6.719651115714072E-4	0.0	0.0	13.182947541363653	0.0
34	6.719651115714072E-4	0.0	0.0	13.498435161246428	0.0
35	6.719651115714072E-4	0.0	0.0	13.819634484577561	0.0
36	6.719651115714072E-4	0.0	0.0	14.14704948519073	0.0
37	6.719651115714072E-4	0.0	0.0	14.491935578704753	0.0
38	8.39956389464259E-4	0.0	0.0	14.851772895951243	0.0
39	8.39956389464259E-4	0.0	0.0	15.224881524151266	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	120	0.0	44.999996	14
CGTCATA	30	2.1637316E-6	44.999996	38
GTATGCG	50	1.0804797E-9	40.500004	1
CATCGTT	50	1.0804797E-9	40.500004	36
CGTTTTT	1740	0.0	40.344826	1
GCTACGA	135	0.0	40.000004	10
TTACGTA	45	1.9255822E-8	40.0	45
TGTTGCG	35	6.2432155E-6	38.571426	1
GACCTAT	130	0.0	38.076927	33
TACGAAT	160	0.0	37.96875	12
GGCGATA	190	0.0	37.894737	8
CTAACGG	60	1.546141E-10	37.499996	2
ATAACGG	30	1.1392608E-4	37.499996	2
AATAGGC	30	1.1392608E-4	37.499996	45
ATCGCAT	30	1.1392608E-4	37.499996	36
CCCGCGA	30	1.1392608E-4	37.499996	28
AATATAC	145	0.0	37.24138	16
CTACGAA	145	0.0	37.24138	11
CGCATGG	115	0.0	37.173912	2
GCCGCAT	55	2.744855E-9	36.81818	19
>>END_MODULE