FastQCFastQC Report
Sat 18 Jun 2016
SRR3550557_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550557_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543634
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17940.3300014347888469No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC14550.26764330413476717Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC12020.22110464025428875Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT11070.2036296478881012Illumina Single End Adapter 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7780.14311099011467274No Hit
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC6860.1261878396126806Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG6720.12361257757976873No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCC6310.11607073876909832No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGT302.1633823E-645.00000444
ATTAGCG351.2103555E-745.01
GCGAAAA253.8880833E-545.01
TAAACGG351.2103555E-745.02
TACGGGT351.2103555E-745.04
ACTAGCG207.030002E-445.01
CGAGGCG207.030002E-445.018
TCTAACG253.8880833E-545.028
TATTACG253.8880833E-545.01
ACGCTTA207.030002E-445.023
TATAGCG253.8880833E-545.01
CGGTCTA800.045.031
CGTCATA253.8880833E-545.038
CGACGGT900.042.528
ACGGTCT850.042.3529430
TAAGTAG650.041.538461
CGTTTTT11750.041.1702161
ATTACGG556.002665E-1140.9090922
AGGGCGT556.002665E-1140.9090926
TCACGAC1000.040.525