SRR3550556_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550556_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	331627
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG	5057	1.524905993782171	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC	4766	1.4371568056883186	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	4673	1.4091132507304893	TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2494	0.752049742632536	TruSeq Adapter, Index 27 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT	1097	0.33079333106170483	No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	978	0.2949096424597515	TruSeq Adapter, Index 27 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	820	0.24726575339161166	TruSeq Adapter, Index 27 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	774	0.23339474771354565	TruSeq Adapter, Index 27 (100% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACCTTTCT	770	0.2321885733067573	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC	756	0.22796696288299806	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTA	707	0.21319132639984079	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	572	0.17248294017073398	No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	522	0.15740576008587961	TruSeq Adapter, Index 27 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	343	0.10342945538210097	TruSeq Adapter, Index 27 (100% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	25	3.8847822E-5	45.000004	25
CTAGATC	20	7.0260186E-4	45.000004	14
GTACATG	20	7.0260186E-4	45.000004	1
CGTATCG	25	3.8847822E-5	45.000004	12
GGTCGTA	25	3.8847822E-5	45.000004	9
CCGCTTA	20	7.0260186E-4	45.000004	19
TACGGGT	20	7.0260186E-4	45.000004	4
ATCGTTC	20	7.0260186E-4	45.000004	40
AATGCTC	20	7.0260186E-4	45.000004	23
CTAAACG	25	3.8847822E-5	45.000004	45
TACGAAT	40	6.7902874E-9	45.000004	12
ACTACGG	20	7.0260186E-4	45.000004	2
GTCGTAT	25	3.8847822E-5	45.000004	10
CGAATAT	40	6.7902874E-9	45.000004	14
CTCGAAT	20	7.0260186E-4	45.000004	43
CGGTCTA	20	7.0260186E-4	45.000004	31
TTGCACG	20	7.0260186E-4	45.000004	1
CACCGAA	25	3.8847822E-5	45.000004	16
GGCTAAT	20	7.0260186E-4	45.000004	38
ACGGTAC	20	7.0260186E-4	45.000004	37