##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550556_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	331627
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.969236521754862	33.0	31.0	34.0	30.0	34.0
2	31.986795405681683	33.0	31.0	34.0	30.0	34.0
3	32.019446546873446	33.0	31.0	34.0	30.0	34.0
4	35.70104062696946	37.0	35.0	37.0	33.0	37.0
5	35.76267613915634	37.0	35.0	37.0	35.0	37.0
6	35.692859749055415	37.0	35.0	37.0	35.0	37.0
7	35.97718219565958	37.0	35.0	37.0	35.0	37.0
8	35.73574829552479	37.0	35.0	37.0	35.0	37.0
9	37.54907471345819	39.0	37.0	39.0	35.0	39.0
10	37.22496961948213	39.0	37.0	39.0	34.0	39.0
11	37.21955691183167	39.0	37.0	39.0	34.0	39.0
12	37.03453578870237	39.0	35.0	39.0	34.0	39.0
13	36.87003169223254	39.0	35.0	39.0	33.0	39.0
14	37.94634634695004	40.0	36.0	41.0	33.0	41.0
15	38.000512624122884	40.0	36.0	41.0	33.0	41.0
16	37.89183932550727	40.0	36.0	41.0	33.0	41.0
17	37.86209808007189	40.0	36.0	41.0	33.0	41.0
18	37.843706935804384	40.0	36.0	41.0	33.0	41.0
19	37.68826422456555	39.0	36.0	41.0	33.0	41.0
20	37.767916966953834	40.0	36.0	41.0	33.0	41.0
21	37.775555066384825	40.0	36.0	41.0	33.0	41.0
22	37.87582434482114	40.0	36.0	41.0	33.0	41.0
23	37.88368558651738	40.0	36.0	41.0	34.0	41.0
24	37.7837419751709	40.0	35.0	41.0	33.0	41.0
25	37.599152059392026	39.0	35.0	41.0	33.0	41.0
26	37.64842729934535	40.0	35.0	41.0	33.0	41.0
27	37.62380324883077	40.0	35.0	41.0	33.0	41.0
28	37.55743953296927	40.0	35.0	41.0	33.0	41.0
29	37.52549400380548	40.0	35.0	41.0	33.0	41.0
30	37.384498246523954	39.0	35.0	41.0	33.0	41.0
31	37.38551746389769	39.0	35.0	41.0	33.0	41.0
32	37.24595403872423	39.0	35.0	41.0	33.0	41.0
33	37.20910842603286	39.0	35.0	41.0	33.0	41.0
34	37.20268856275273	40.0	35.0	41.0	33.0	41.0
35	37.07868478742684	39.0	35.0	41.0	32.0	41.0
36	36.994433505112674	39.0	35.0	41.0	32.0	41.0
37	36.94325552503264	39.0	35.0	41.0	32.0	41.0
38	36.83203418298269	39.0	35.0	41.0	31.0	41.0
39	36.78104919080774	39.0	35.0	41.0	31.0	41.0
40	36.618658914985815	39.0	35.0	41.0	31.0	41.0
41	36.49998944597394	39.0	35.0	41.0	31.0	41.0
42	36.57859884750035	39.0	35.0	41.0	31.0	41.0
43	36.47673138797504	39.0	35.0	41.0	31.0	41.0
44	36.34649470640207	39.0	35.0	41.0	31.0	41.0
45	36.32479562882395	38.0	35.0	40.0	31.0	41.0
46	36.1500722196926	38.0	35.0	40.0	30.0	41.0
47	35.98808601229695	38.0	35.0	40.0	30.0	41.0
48	35.95627919318994	38.0	35.0	40.0	30.0	41.0
49	35.97655800040407	38.0	35.0	40.0	30.0	41.0
50	35.80272414489773	38.0	35.0	40.0	30.0	41.0
51	34.702527236925825	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	10.0
11	5.0
12	4.0
13	8.0
14	5.0
15	10.0
16	27.0
17	46.0
18	93.0
19	174.0
20	354.0
21	562.0
22	948.0
23	1291.0
24	1641.0
25	2073.0
26	2436.0
27	2980.0
28	3289.0
29	3935.0
30	4927.0
31	6410.0
32	8654.0
33	11872.0
34	23508.0
35	32126.0
36	24527.0
37	35487.0
38	63835.0
39	100331.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.44901048467103	19.213755212935013	22.053089766514788	10.284144535879165
2	30.260201973904415	28.400884125840175	23.69409004695034	17.64482385330507
3	30.21105036682779	28.898732612242068	24.996758406281756	15.893458614648384
4	26.518950507648654	23.98085801216427	30.608786377466252	18.891405102720828
5	28.833599194275493	27.67989337418244	22.900427287283602	20.58608014425846
6	24.654204874753866	36.74188169238331	24.070718005470003	14.533195427392823
7	75.32498861672904	5.5481007276247105	14.232858000102524	4.894052655543728
8	74.0241295190078	9.816450409646983	10.04954361375883	6.109876457586385
9	69.53022522291612	6.472030323224587	11.2433547328776	12.754389720981704
10	41.39439792296768	23.970002442503173	18.58986150102374	16.045738133505413
11	34.74415533114011	21.67495408998664	23.818627554451236	19.762263024422015
12	29.48644109194969	20.160602122263867	28.219656421220225	22.133300364566214
13	24.89483666890814	24.67832836288963	31.534826778278006	18.892008189924223
14	21.220226338627434	29.94629508453775	27.231196494857173	21.602282081977645
15	18.392953529115545	23.25082095245562	37.379043322769256	20.977182195659584
16	20.910541059684526	24.326426979709133	30.159787954539286	24.603244006067058
17	20.536928537181833	25.159290407596487	29.533783437416133	24.769997617805547
18	23.365407521100515	23.290624707879637	29.7234543628836	23.620513408136247
19	23.115729418895327	26.71193841273479	25.579943731963922	24.59238843640596
20	26.425471991122556	25.610098092133633	28.59236431291783	19.372065603825988
21	24.053530020173266	30.314479822209893	26.2035359002735	19.42845425734334
22	22.115810835667784	23.4082267125415	27.676877938165468	26.79908451362525
23	21.742499856766788	28.055616701897012	27.520678352486378	22.681205088849822
24	24.561329445431163	23.220666592285912	27.50107801837607	24.71692594390686
25	20.715140805784813	28.87370449330121	26.025022088068823	24.386132612845156
26	20.440434584638766	24.604148636872146	28.440687881264193	26.51472889722489
27	22.766843471731796	27.488413187104786	25.228946979588514	24.5157963615749
28	18.245800251487367	26.77797646150645	29.1022745433876	25.873948743618584
29	21.594743491935215	23.801138025552802	26.015071149212822	28.589047333299156
30	21.42859296740012	27.76372249545423	27.982341606684617	22.82534293046103
31	23.987491971401607	24.35658133987884	24.10509397606347	27.55083271265609
32	24.610481052507787	25.44153521878496	25.008217063146244	24.939766665561006
33	22.415243632152993	23.496880531440446	26.15528892400197	27.9325869124046
34	20.45460713391853	22.252711630838263	30.186625335090326	27.10605590015288
35	18.38059024144596	25.32544093213158	29.319989023812898	26.97397980260956
36	20.13587554692471	26.067539735908113	26.184538653366584	27.612046063800594
37	19.799654431032454	26.58227466400504	28.628851088723174	24.98921981623933
38	19.411266272046607	26.703796735488964	26.053668730230044	27.831268262234378
39	22.391723231220617	21.99820883100592	28.567336193976967	27.042731743796494
40	21.952977290751356	21.480156923290323	32.86342788735537	23.70343789860295
41	18.411347688819067	24.500719181490048	27.681099548589227	29.40683358110166
42	20.66659228591158	22.291912299058882	29.90046045707979	27.141034957949746
43	23.044263585293116	22.095607414354077	25.41077777141186	29.44935122894095
44	21.666209325537427	22.870574470715592	27.564703718334155	27.89851248541283
45	20.876165089091057	21.7518477084194	27.56138673871548	29.810600463774062
46	24.3191899332684	24.115044914919473	26.590114797649168	24.97565035416296
47	18.7388240402621	22.525608590374123	33.623920850835425	25.111646518528346
48	21.10865520599951	23.10366767482744	28.567637737578664	27.22003938159438
49	20.950646358710237	19.640137865734697	33.30157074062124	26.107645034933824
50	20.608092827182347	21.331194384051962	30.665175030983605	27.395537757782083
51	19.766484634845778	21.018493669092084	27.43262762079083	31.782394075271313
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	320.5
2	492.0
3	883.5
4	1275.0
5	899.0
6	523.0
7	506.0
8	489.0
9	521.0
10	553.0
11	559.5
12	566.0
13	631.0
14	696.0
15	651.5
16	607.0
17	602.5
18	598.0
19	597.5
20	597.0
21	707.0
22	817.0
23	811.5
24	806.0
25	993.0
26	1414.0
27	1648.0
28	2093.0
29	2538.0
30	2691.5
31	2845.0
32	3641.5
33	4438.0
34	5179.0
35	5920.0
36	6489.0
37	7058.0
38	7718.0
39	8378.0
40	9767.5
41	11157.0
42	14014.5
43	16872.0
44	18718.0
45	20564.0
46	32412.0
47	44260.0
48	39575.0
49	34890.0
50	34765.5
51	34641.0
52	29989.0
53	25337.0
54	22790.0
55	20243.0
56	18923.0
57	17603.0
58	16724.5
59	15846.0
60	15119.0
61	14392.0
62	12903.0
63	11414.0
64	9748.0
65	8082.0
66	6572.5
67	5063.0
68	4193.0
69	3323.0
70	2789.5
71	2256.0
72	1955.5
73	1655.0
74	1263.0
75	684.5
76	498.0
77	396.5
78	295.0
79	190.0
80	85.0
81	74.0
82	63.0
83	39.0
84	15.0
85	14.0
86	13.0
87	10.0
88	7.0
89	5.5
90	4.0
91	2.5
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	331627.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.669223374168304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.83234033350047	22.030420911774048
2	10.284740655756023	6.308500170335433
3	3.5723893494416776	3.2868722081257977
4	1.8531919607354053	2.273438327960283
5	1.2544311897864873	1.9236215183542742
6	0.9685597074974897	1.7822984412275773
7	0.8516128057795749	1.8282812358129192
8	0.7536573384853108	1.8491268209269731
9	0.682689604799257	1.8843803986329983
>10	7.766468856764326	44.89926388148892
>50	0.12694228375919514	2.678352292578722
>100	0.03998182165643942	1.9024670170245566
>500	0.007996364331287883	1.8083552905942408
>1k	0.003998182165643942	3.9943836983290315
>5k	9.995455414109854E-4	1.550237786834222
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG	5057	1.524905993782171	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC	4766	1.4371568056883186	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	4673	1.4091132507304893	TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2494	0.752049742632536	TruSeq Adapter, Index 27 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT	1097	0.33079333106170483	No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	978	0.2949096424597515	TruSeq Adapter, Index 27 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	820	0.24726575339161166	TruSeq Adapter, Index 27 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	774	0.23339474771354565	TruSeq Adapter, Index 27 (100% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACCTTTCT	770	0.2321885733067573	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC	756	0.22796696288299806	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTA	707	0.21319132639984079	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	572	0.17248294017073398	No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	522	0.15740576008587961	TruSeq Adapter, Index 27 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	343	0.10342945538210097	TruSeq Adapter, Index 27 (100% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.030872033941747E-4	0.0	0.0	0.2529950818238563	0.0
2	6.030872033941747E-4	0.0	0.0	1.6624098761560429	0.0
3	6.030872033941747E-4	0.0	0.0	2.089697159760816	0.0
4	6.030872033941747E-4	0.0	0.0	2.915323541207441	0.0
5	6.030872033941747E-4	0.0	0.0	5.913270029279884	0.0
6	6.030872033941747E-4	0.0	0.0	6.830565665642424	0.0
7	6.030872033941747E-4	0.0	0.0	8.065386714591996	0.0
8	6.030872033941747E-4	0.0	0.0	9.641856664264369	0.0
9	6.030872033941747E-4	0.0	0.0	10.168351792827483	0.0
10	6.030872033941747E-4	0.0	0.0	12.770069988269954	0.0
11	6.030872033941747E-4	0.0	0.0	14.231953369297434	0.0
12	6.030872033941747E-4	0.0	0.0	16.862318206901126	0.0
13	6.030872033941747E-4	0.0	0.0	17.415047628811887	0.0
14	6.030872033941747E-4	0.0	0.0	17.6415068736864	0.0
15	6.030872033941747E-4	0.0	0.0	18.216550522122745	0.0
16	6.030872033941747E-4	0.0	0.0	18.98970831687408	0.0
17	6.030872033941747E-4	0.0	0.0	19.87986502908388	0.0
18	6.030872033941747E-4	0.0	0.0	20.78238502896326	0.0
19	6.030872033941747E-4	0.0	0.0	21.562779870155325	0.0
20	6.030872033941747E-4	0.0	0.0	22.107669158421963	0.0
21	6.030872033941747E-4	0.0	0.0	22.72281810588402	0.0
22	6.030872033941747E-4	0.0	0.0	23.42993785186369	0.0
23	6.030872033941747E-4	0.0	0.0	24.087604447165038	0.0
24	6.030872033941747E-4	0.0	0.0	24.58334212835505	0.0
25	6.030872033941747E-4	0.0	0.0	25.029928202468437	0.0
26	6.030872033941747E-4	0.0	0.0	25.468975686539395	0.0
27	6.030872033941747E-4	0.0	0.0	25.91887874027145	0.0
28	6.030872033941747E-4	0.0	0.0	26.325359515359125	0.0
29	6.030872033941747E-4	0.0	0.0	26.763200825023294	0.0
30	6.030872033941747E-4	0.0	0.0	27.304471590069568	0.0
31	6.030872033941747E-4	0.0	0.0	27.787845983589996	0.0
32	6.030872033941747E-4	0.0	0.0	28.216942528804953	0.0
33	6.030872033941747E-4	0.0	0.0	28.670162562155674	0.0
34	6.030872033941747E-4	0.0	0.0	29.062470787963587	0.0
35	6.030872033941747E-4	0.0	0.0	29.54463900707723	0.0
36	6.030872033941747E-4	0.0	0.0	29.937248776486836	0.0
37	6.030872033941747E-4	0.0	0.0	30.3654406908967	0.0
38	6.030872033941747E-4	0.0	0.0	30.805091262171054	0.0
39	6.030872033941747E-4	0.0	0.0	31.25710512111499	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	25	3.8847822E-5	45.000004	25
CTAGATC	20	7.0260186E-4	45.000004	14
GTACATG	20	7.0260186E-4	45.000004	1
CGTATCG	25	3.8847822E-5	45.000004	12
GGTCGTA	25	3.8847822E-5	45.000004	9
CCGCTTA	20	7.0260186E-4	45.000004	19
TACGGGT	20	7.0260186E-4	45.000004	4
ATCGTTC	20	7.0260186E-4	45.000004	40
AATGCTC	20	7.0260186E-4	45.000004	23
CTAAACG	25	3.8847822E-5	45.000004	45
TACGAAT	40	6.7902874E-9	45.000004	12
ACTACGG	20	7.0260186E-4	45.000004	2
GTCGTAT	25	3.8847822E-5	45.000004	10
CGAATAT	40	6.7902874E-9	45.000004	14
CTCGAAT	20	7.0260186E-4	45.000004	43
CGGTCTA	20	7.0260186E-4	45.000004	31
TTGCACG	20	7.0260186E-4	45.000004	1
CACCGAA	25	3.8847822E-5	45.000004	16
GGCTAAT	20	7.0260186E-4	45.000004	38
ACGGTAC	20	7.0260186E-4	45.000004	37
>>END_MODULE