SRR3550555_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550555_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	396502
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	11433	2.8834659093775064	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	11077	2.793680738054285	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	10450	2.635547866088948	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	2896	0.730387236382162	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTGTGGT	2619	0.660526302515498	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCT	2278	0.5745242142536481	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTA	1667	0.4204266308871078	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	1650	0.4161391367508865	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTC	1475	0.37200316770155006	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1209	0.30491649474655863	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	751	0.18940635860600954	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT	654	0.16494242147580593	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	575	0.14501818401924832	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATG	501	0.12635497424981465	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCTCCGGTCGAATGC	499	0.12585056317496507	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGTTGTGG	482	0.12156306903874381	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	480	0.12105865796389426	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	468	0.1180321915147969	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGT	438	0.11046602539205351	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGGT	115	0.0	45.000004	38
ATAGGCG	20	7.02769E-4	45.000004	36
CACGACG	20	7.02769E-4	45.000004	26
CGTACTA	20	7.02769E-4	45.000004	45
CCGACAC	20	7.02769E-4	45.000004	23
ATGCGCG	30	2.1618907E-6	45.000004	1
ATAACGC	20	7.02769E-4	45.000004	11
CGGTACA	20	7.02769E-4	45.000004	30
AACAGCG	20	7.02769E-4	45.000004	1
CTCGTAG	35	1.2092278E-7	45.0	1
GTCGAAT	100	0.0	45.0	43
CTCCGAT	25	3.886169E-5	45.0	38
CTCCCGT	35	1.2092278E-7	45.0	38
GTTAGCG	35	1.2092278E-7	45.0	1
CGCAATT	25	3.886169E-5	45.0	43
GATGAAT	1315	0.0	43.63118	20
TCGAATG	120	0.0	43.125004	44
CGATGAA	1280	0.0	43.06641	19
ACGGCTG	1235	0.0	42.63158	8
GGTCGAA	90	0.0	42.5	42

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content