##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550555_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	396502
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96449450444134	33.0	31.0	34.0	30.0	34.0
2	31.991566246828516	33.0	31.0	34.0	30.0	34.0
3	32.02443115041034	33.0	31.0	34.0	30.0	34.0
4	35.72052852192423	37.0	35.0	37.0	35.0	37.0
5	35.73602655219898	37.0	35.0	37.0	35.0	37.0
6	35.66360573212746	37.0	35.0	37.0	35.0	37.0
7	35.87057568435973	37.0	35.0	37.0	35.0	37.0
8	35.55458988857559	37.0	35.0	37.0	33.0	37.0
9	37.29310823148433	39.0	37.0	39.0	34.0	39.0
10	37.14680127716884	39.0	37.0	39.0	34.0	39.0
11	37.13256679663659	39.0	37.0	39.0	34.0	39.0
12	36.9925170617046	39.0	35.0	39.0	33.0	39.0
13	36.8042758926815	39.0	35.0	39.0	33.0	39.0
14	37.96996484254808	40.0	37.0	41.0	33.0	41.0
15	37.92422484628072	40.0	36.0	41.0	33.0	41.0
16	37.94265098284498	40.0	36.0	41.0	33.0	41.0
17	37.74578690649732	40.0	36.0	41.0	32.0	41.0
18	37.764167646064834	40.0	36.0	41.0	33.0	41.0
19	37.62177239963481	40.0	36.0	41.0	33.0	41.0
20	37.34410923526237	40.0	35.0	41.0	32.0	41.0
21	37.63453904393925	40.0	36.0	41.0	33.0	41.0
22	37.650450691295376	40.0	36.0	41.0	33.0	41.0
23	37.55862265511902	40.0	36.0	41.0	33.0	41.0
24	37.57490504461516	40.0	35.0	41.0	33.0	41.0
25	37.543505455205775	39.0	35.0	41.0	33.0	41.0
26	37.38271938098673	39.0	35.0	41.0	32.0	41.0
27	37.253360638786184	40.0	35.0	41.0	32.0	41.0
28	37.17172170632178	39.0	35.0	41.0	32.0	41.0
29	37.07483947117543	39.0	35.0	41.0	31.0	41.0
30	37.00842870906074	39.0	35.0	41.0	31.0	41.0
31	36.94594478716375	39.0	35.0	41.0	31.0	41.0
32	36.6910330843224	39.0	35.0	41.0	31.0	41.0
33	36.671181986471694	39.0	35.0	41.0	30.0	41.0
34	36.286835879768574	39.0	35.0	41.0	30.0	41.0
35	36.32164781009932	39.0	35.0	41.0	30.0	41.0
36	36.09887213683664	39.0	35.0	41.0	29.0	41.0
37	35.97813882401602	39.0	35.0	41.0	29.0	41.0
38	35.87357440819971	39.0	35.0	41.0	29.0	41.0
39	35.82804626458378	39.0	35.0	41.0	29.0	41.0
40	35.73882351160902	39.0	35.0	41.0	28.0	41.0
41	35.63723764318969	38.0	35.0	41.0	26.0	41.0
42	35.59492007606519	38.0	35.0	41.0	27.0	41.0
43	35.524885120377704	38.0	35.0	41.0	27.0	41.0
44	35.566156034521896	38.0	35.0	40.0	27.0	41.0
45	35.62707880414222	38.0	35.0	40.0	27.0	41.0
46	35.587656556587355	38.0	35.0	40.0	27.0	41.0
47	35.28477536052782	38.0	35.0	40.0	26.0	41.0
48	35.35062370429405	38.0	35.0	40.0	26.0	41.0
49	35.32439937251262	38.0	35.0	40.0	27.0	41.0
50	35.12780263403463	38.0	35.0	40.0	26.0	41.0
51	33.91145820197628	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	6.0
10	7.0
11	6.0
12	8.0
13	7.0
14	7.0
15	7.0
16	27.0
17	62.0
18	166.0
19	487.0
20	1079.0
21	2136.0
22	3070.0
23	3434.0
24	3766.0
25	3748.0
26	4153.0
27	4374.0
28	4441.0
29	4836.0
30	6016.0
31	8048.0
32	10980.0
33	14701.0
34	25822.0
35	33731.0
36	28820.0
37	42412.0
38	71271.0
39	118790.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.74652334666659	17.031692147832796	20.26900242621727	10.952782079283333
2	27.16606725817272	37.35113568153503	20.72852091540522	14.754276144887038
3	27.49166460698811	36.00713237259837	22.925987762987322	13.575215257426192
4	25.090415685166782	20.69800404537682	38.15441031823295	16.05716995122345
5	34.932988988706235	25.14287443695114	21.34112816581001	18.583008408532617
6	23.183994027772876	41.60231222036711	22.196609348754862	13.017084403105155
7	69.1121356260498	5.411574216523498	21.0346984378389	4.441591719587795
8	67.52046647936201	17.134087596027257	10.340174828878542	5.005271095732178
9	63.00422192069649	6.906396436840168	10.227186748112242	19.862194894351102
10	44.31755703628229	21.78854078920157	18.346439614428174	15.547462560087968
11	36.418479604138184	20.896489803330123	23.371887153154336	19.313143439377356
12	27.860641308240563	19.32676253839829	27.231640697903163	25.580955455457982
13	26.713862729570092	22.471261179010448	33.126440724132536	17.688435367286925
14	20.483881544103184	32.327705787108265	25.29293673171888	21.895475937069676
15	17.81529475261159	22.080594801539462	36.93676198354611	23.16734846230284
16	19.51440345824233	24.187267655648647	26.270989805852178	30.027339080256848
17	19.80998834810417	27.00717776959511	29.928474509586334	23.254359372714386
18	20.42738750372003	24.369864464744186	28.290147338474963	26.912600693060817
19	21.672274036448744	25.64602448411357	25.002900363680386	27.678801115757295
20	26.535301209073346	24.863430701484482	30.427337062612548	18.173931026829628
21	22.881095177325715	32.66490459064519	25.43064095515281	19.023359276876285
22	20.566857165915938	22.411740672178198	30.27979682321905	26.741605338686814
23	24.161794896368743	28.029366812777738	27.12016585036141	20.688672440492102
24	23.351458504622926	25.604914981513332	25.462923263943182	25.580703249920557
25	20.337097921321963	34.39654781060373	23.904797453732893	21.361556814341416
26	19.963329314858434	24.148680208422658	30.516870028398348	25.37112044832056
27	26.992549848424474	25.711597923844014	24.517404704137686	22.77844752359383
28	17.987298929135285	28.223817282132245	31.282566040019976	22.50631774871249
29	23.396603295821965	25.410212306621403	28.779930492153888	22.413253905402748
30	23.795592456027965	29.421289173825098	27.274011228190524	19.50910714195641
31	24.054859748500636	23.418040766503072	26.596587154667567	25.930512330328725
32	29.024569863455923	26.653585606125567	26.346399261542185	17.97544526887632
33	28.49468602932646	22.632168311887455	23.544647946290308	25.328497712495775
34	23.476804656723044	25.67099283231863	29.147141754644363	21.705060756313966
35	30.07374489914301	22.173406439311783	24.692939758185332	23.059908903359883
36	25.37566014799421	29.578413223640737	25.650564183787218	19.39536244457783
37	25.302772747678446	23.599880958986336	31.877771108342458	19.219575184992763
38	25.287892620970386	24.451074647794968	25.805922794840885	24.45510993639376
39	24.257885206127586	24.02131641202314	31.22909846608592	20.491699915763352
40	27.465939642170785	22.368361319741137	27.6187761978502	22.54692284023788
41	23.64754780555962	24.584491377092675	27.099232790755153	24.66872802659255
42	24.9572511614065	22.765080630110315	30.030869957780794	22.246798250702394
43	28.511836005871345	22.65310137149371	24.263938139025782	24.57112448360916
44	24.588778871228897	22.015273567346444	28.762528310071577	24.633419251353082
45	21.77063419604441	21.307080418257662	28.170349708198195	28.751935677499734
46	27.638700435306756	26.625590791471414	25.347917538877486	20.38779123434434
47	21.736586448492062	23.273526993558672	34.528451306676885	20.461435251272377
48	23.325985745343026	25.056872348689286	26.872500012610278	24.74464189335741
49	22.387781146122844	21.40140528925453	33.48356376512603	22.727249799496597
50	24.830896187156686	21.57214843809111	28.84096423221068	24.755991142541525
51	22.352472370883376	22.574413243817183	26.472754235792	28.60036014950744
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	468.5
2	791.0
3	1563.5
4	2336.0
5	1604.0
6	872.0
7	839.0
8	806.0
9	832.0
10	858.0
11	895.5
12	933.0
13	936.0
14	939.0
15	909.5
16	880.0
17	839.5
18	799.0
19	838.0
20	877.0
21	950.5
22	1024.0
23	1176.5
24	1329.0
25	1572.5
26	2114.5
27	2413.0
28	3194.5
29	3976.0
30	4458.0
31	4940.0
32	5787.0
33	6634.0
34	7490.5
35	8347.0
36	8567.5
37	8788.0
38	9855.0
39	10922.0
40	12772.0
41	14622.0
42	15770.5
43	16919.0
44	19543.5
45	22168.0
46	45209.5
47	68251.0
48	51465.0
49	34679.0
50	33719.5
51	32760.0
52	28868.5
53	24977.0
54	23381.5
55	21786.0
56	21395.5
57	21005.0
58	19893.5
59	18782.0
60	17528.0
61	16274.0
62	15022.0
63	13770.0
64	11853.5
65	9937.0
66	8380.0
67	6823.0
68	5975.0
69	5127.0
70	4196.5
71	3266.0
72	2585.5
73	1905.0
74	1575.0
75	1077.0
76	909.0
77	686.0
78	463.0
79	350.5
80	238.0
81	157.5
82	77.0
83	70.0
84	63.0
85	36.5
86	10.0
87	11.0
88	12.0
89	8.0
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	396502.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.161640406428287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.14805938167154	21.331174041163685
2	7.954781042792488	4.639489285635744
3	2.963731698180261	2.5928183413039747
4	1.7982683980770495	2.0976182551586704
5	1.4441537279633834	2.1056945853235525
6	1.181479775463165	2.067233301571467
7	1.0567200376693329	2.1570982824146308
8	1.003012984189485	2.3399603174329844
9	0.8401742185370034	2.205077259575519
>10	8.477962594352853	42.024268623912526
>50	0.08141892936659599	1.6188244515787813
>100	0.03811098821415132	2.384665571439425
>500	0.0034646352921955744	0.6266661670099792
>1k	0.006063111761342255	3.4841729575718072
>5k	0.0	0.0
>10k+	0.0025984764691466804	8.32523855890726
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	11433	2.8834659093775064	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	11077	2.793680738054285	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	10450	2.635547866088948	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	2896	0.730387236382162	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTGTGGT	2619	0.660526302515498	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCT	2278	0.5745242142536481	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTA	1667	0.4204266308871078	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	1650	0.4161391367508865	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTC	1475	0.37200316770155006	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1209	0.30491649474655863	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	751	0.18940635860600954	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT	654	0.16494242147580593	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	575	0.14501818401924832	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATG	501	0.12635497424981465	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCTCCGGTCGAATGC	499	0.12585056317496507	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGTTGTGG	482	0.12156306903874381	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	480	0.12105865796389426	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	468	0.1180321915147969	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGT	438	0.11046602539205351	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15182773352971737	0.0
2	0.0	0.0	0.0	1.222692445435332	0.0
3	0.0	0.0	0.0	1.4685928444244922	0.0
4	0.0	0.0	0.0	2.1518176453082205	0.0
5	0.0	0.0	0.0	5.61586070183757	0.0
6	0.0	0.0	0.0	6.220649580582191	0.0
7	0.0	0.0	0.0	7.233254813342682	0.0
8	0.0	0.0	0.0	8.154813847092827	0.0
9	0.0	0.0	0.0	8.387851763673323	0.0
10	0.0	0.0	0.0	11.84861614821615	0.0
11	0.0	0.0	0.0	12.606745993715037	0.0
12	0.0	0.0	0.0	16.11795148574282	0.0
13	0.0	0.0	0.0	16.493989942043168	0.0
14	0.0	0.0	0.0	16.70407715471801	0.0
15	0.0	0.0	0.0	17.33988731456588	0.0
16	0.0	0.0	0.0	17.85867410504865	0.0
17	0.0	0.0	0.0	18.354762397163192	0.0
18	0.0	0.0	0.0	18.834457329345124	0.0
19	0.0	0.0	0.0	19.900530136039666	0.0
20	2.522055374247797E-4	0.0	0.0	20.32448764445072	0.0
21	2.522055374247797E-4	0.0	0.0	20.689176851566952	0.0
22	2.522055374247797E-4	0.0	0.0	21.22511361859461	0.0
23	2.522055374247797E-4	0.0	0.0	21.610231474242248	0.0
24	2.522055374247797E-4	0.0	0.0	21.96382363771179	0.0
25	2.522055374247797E-4	0.0	0.0	22.27302762659457	0.0
26	2.522055374247797E-4	0.0	0.0	22.53380815229179	0.0
27	2.522055374247797E-4	0.0	0.0	22.942381122919937	0.0
28	2.522055374247797E-4	0.0	0.0	23.22333809161114	0.0
29	5.044110748495594E-4	0.0	0.0	23.512365637499936	0.0
30	5.044110748495594E-4	0.0	0.0	23.8397284250773	0.0
31	5.044110748495594E-4	0.0	0.0	24.14363609767416	0.0
32	5.044110748495594E-4	0.0	0.0	24.431907026950682	0.0
33	5.044110748495594E-4	0.0	0.0	24.721186778376907	0.0
34	5.044110748495594E-4	0.0	0.0	25.014501818401925	0.0
35	5.044110748495594E-4	0.0	0.0	25.3307675623326	0.0
36	5.044110748495594E-4	0.0	0.0	25.60995909226183	0.0
37	5.044110748495594E-4	0.0	0.0	25.914118970396114	0.0
38	5.044110748495594E-4	0.0	0.0	26.200624460910664	0.0
39	5.044110748495594E-4	0.0	0.0	26.475528496703674	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGGT	115	0.0	45.000004	38
ATAGGCG	20	7.02769E-4	45.000004	36
CACGACG	20	7.02769E-4	45.000004	26
CGTACTA	20	7.02769E-4	45.000004	45
CCGACAC	20	7.02769E-4	45.000004	23
ATGCGCG	30	2.1618907E-6	45.000004	1
ATAACGC	20	7.02769E-4	45.000004	11
CGGTACA	20	7.02769E-4	45.000004	30
AACAGCG	20	7.02769E-4	45.000004	1
CTCGTAG	35	1.2092278E-7	45.0	1
GTCGAAT	100	0.0	45.0	43
CTCCGAT	25	3.886169E-5	45.0	38
CTCCCGT	35	1.2092278E-7	45.0	38
GTTAGCG	35	1.2092278E-7	45.0	1
CGCAATT	25	3.886169E-5	45.0	43
GATGAAT	1315	0.0	43.63118	20
TCGAATG	120	0.0	43.125004	44
CGATGAA	1280	0.0	43.06641	19
ACGGCTG	1235	0.0	42.63158	8
GGTCGAA	90	0.0	42.5	42
>>END_MODULE