##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550553_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431375
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18782034192988	33.0	31.0	34.0	30.0	34.0
2	32.207135323094754	33.0	31.0	34.0	30.0	34.0
3	32.225919443639526	34.0	31.0	34.0	30.0	34.0
4	35.877118516372065	37.0	35.0	37.0	35.0	37.0
5	35.92759200231817	37.0	35.0	37.0	35.0	37.0
6	35.86248623587366	37.0	35.0	37.0	35.0	37.0
7	36.1025975079687	37.0	35.0	37.0	35.0	37.0
8	35.8909046653144	37.0	35.0	37.0	35.0	37.0
9	37.73758794552304	39.0	38.0	39.0	35.0	39.0
10	37.4283071573457	39.0	37.0	39.0	34.0	39.0
11	37.41199884091568	39.0	37.0	39.0	35.0	39.0
12	37.28464792813677	39.0	37.0	39.0	35.0	39.0
13	37.21125239061141	39.0	37.0	39.0	34.0	39.0
14	38.41013503332368	40.0	38.0	41.0	34.0	41.0
15	38.49045030425963	40.0	38.0	41.0	34.0	41.0
16	38.31052332657201	40.0	37.0	41.0	34.0	41.0
17	38.338049261083746	40.0	37.0	41.0	34.0	41.0
18	38.26973283106346	40.0	37.0	41.0	34.0	41.0
19	38.14426427122573	40.0	37.0	41.0	34.0	41.0
20	38.23815473775717	40.0	37.0	41.0	34.0	41.0
21	38.1901709649377	40.0	37.0	41.0	34.0	41.0
22	38.25909011880614	40.0	37.0	41.0	34.0	41.0
23	38.26710634598667	40.0	37.0	41.0	34.0	41.0
24	38.183459866705306	40.0	37.0	41.0	34.0	41.0
25	37.94326282237033	40.0	36.0	41.0	34.0	41.0
26	37.98319095914228	40.0	36.0	41.0	34.0	41.0
27	37.97864734859461	40.0	36.0	41.0	34.0	41.0
28	37.88995653433787	40.0	36.0	41.0	33.0	41.0
29	37.85165575195595	40.0	36.0	41.0	33.0	41.0
30	37.70313068675746	40.0	36.0	41.0	33.0	41.0
31	37.694062011011304	40.0	36.0	41.0	33.0	41.0
32	37.60766850188351	40.0	36.0	41.0	33.0	41.0
33	37.52847522457259	40.0	36.0	41.0	33.0	41.0
34	37.42298232396407	40.0	35.0	41.0	33.0	41.0
35	37.33795885250652	40.0	35.0	41.0	33.0	41.0
36	37.23170327441321	40.0	35.0	41.0	33.0	41.0
37	37.17555259345117	40.0	35.0	41.0	33.0	41.0
38	37.03763314981165	40.0	35.0	41.0	32.0	41.0
39	36.957354969574034	39.0	35.0	41.0	32.0	41.0
40	36.835050709939146	39.0	35.0	41.0	31.0	41.0
41	36.70473254129238	39.0	35.0	41.0	31.0	41.0
42	36.686323964068386	39.0	35.0	41.0	31.0	41.0
43	36.619808751086644	39.0	35.0	41.0	31.0	41.0
44	36.53225152129817	39.0	35.0	41.0	31.0	41.0
45	36.49349869603014	39.0	35.0	41.0	31.0	41.0
46	36.31898232396407	39.0	35.0	41.0	31.0	41.0
47	36.186964937699216	38.0	35.0	41.0	30.0	41.0
48	36.0940272384816	38.0	35.0	41.0	30.0	41.0
49	36.0521240220226	38.0	35.0	40.0	30.0	41.0
50	35.93652854245146	38.0	35.0	40.0	30.0	41.0
51	34.74756766154738	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	11.0
10	12.0
11	8.0
12	11.0
13	8.0
14	11.0
15	28.0
16	41.0
17	50.0
18	128.0
19	265.0
20	480.0
21	771.0
22	1127.0
23	1511.0
24	1856.0
25	2485.0
26	3158.0
27	3754.0
28	4052.0
29	4740.0
30	5550.0
31	7120.0
32	9426.0
33	13436.0
34	25391.0
35	35413.0
36	31679.0
37	47506.0
38	81797.0
39	149449.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.2007534048102	20.71608229498696	23.694233555491163	11.38893074471168
2	30.93735149232107	26.93642422486236	24.860967835410026	17.26525644740655
3	32.351898000579546	25.969516082294987	25.56870472326862	16.109881193856854
4	28.8361634308896	25.08443929295856	27.33561286583599	18.74378441031585
5	27.720197044334977	29.985047812228338	23.054882642712258	19.239872500724427
6	25.41361924079977	37.22839756592292	22.987655751955955	14.370327441321356
7	78.6589394378441	6.550217328310634	10.194842074760938	4.596001159084323
8	79.81895102868734	8.051231527093595	8.085772239930456	4.044045204288612
9	73.45325992465952	8.299739206027239	9.651926977687626	8.595073891625615
10	42.53491741524196	25.69064039408867	16.29579831932773	15.47864387134164
11	33.36238771370617	24.24665314401623	22.397450014488555	19.993509127789046
12	29.199188640973635	22.656621269197334	26.712952767313823	21.431237322515212
13	25.786380759200235	24.174094465372356	29.850594030715733	20.188930744711676
14	21.946334395827297	28.072558678643873	27.952941176470585	22.028165749058243
15	20.692900608519267	25.379542161692264	32.723964068385975	21.20359316140249
16	23.175659229208925	24.643755433207765	29.291451753115037	22.889133584468272
17	23.298058533758333	25.118748188930745	27.85557809330629	23.727615184004634
18	24.08762677484787	25.23025210084033	27.648797450014488	23.033323674297304
19	26.340423065778033	25.4935960591133	25.22097942625326	22.945001448855404
20	27.431816864676904	25.730976528542453	26.62161692263112	20.21558968414952
21	26.00289771080846	27.31382208055636	26.011706751666186	20.671573456968993
22	24.827354390031875	24.12865835989568	26.454476963199074	24.58951028687337
23	24.90617212402202	26.102115328890175	26.54372645609968	22.44798609098812
24	24.20585337583309	25.188756882063167	27.54378441031585	23.061605331787888
25	23.52941176470588	27.8514053897421	25.473196175021734	23.14598667053028
26	22.52726745870762	27.15247754274123	26.75792523906114	23.56232975949
27	24.09504491451753	26.960764995653435	26.285018835120255	22.65917125470878
28	22.342741234424803	27.165691104027818	28.321993625036225	22.169574036511154
29	23.398667053028106	26.539321935670817	26.83396117067517	23.228049840625907
30	23.310344827586206	28.426543031005508	26.589858012170385	21.6732541292379
31	26.75954795711388	26.2996232975949	23.761460446247465	23.179368299043755
32	25.9192118226601	28.403129527673137	23.83796001159084	21.83969863807592
33	24.469892784700086	26.95218777166039	24.85864966676326	23.719269776876267
34	24.287452912199363	27.35044914517531	25.29631990727325	23.06577803535207
35	24.605273833671397	27.106114169805856	24.83755433207766	23.451057664445088
36	25.073543900318747	29.243233845262246	23.72274702984642	21.960475224572587
37	24.66600985221675	29.358678643871343	24.912199362503625	21.06311214140829
38	24.297884671109824	27.471689365401332	24.920776586496668	23.309649376992176
39	24.74830483917705	26.74447986090988	25.860562155896844	22.646653144016227
40	24.734627644161115	25.580063749637787	27.746160533178788	21.939148073022313
41	23.311272095044913	26.519385685308606	25.64126340191249	24.528078817733988
42	23.58574326282237	26.28385975079687	26.084729064039408	24.04566792234135
43	23.107273254129236	25.49405969284265	26.29150970733121	25.1071573456969
44	23.18145465082585	25.981802376122864	26.800115908432335	24.03662706461895
45	23.24474065488264	25.691104027818024	25.98504781222834	25.079107505070997
46	24.682932483338163	27.20672268907563	25.30396986380759	22.806374963778612
47	23.48838017965807	25.343842364532023	28.00208635178209	23.165691104027818
48	23.222718052738337	26.01402492031295	26.821906693711966	23.941350333236745
49	22.567603593161405	25.87632570269487	28.03083164300203	23.525239061141697
50	22.052042886119967	25.287047232686177	27.8323964068386	24.82851347435526
51	22.47418139669661	25.877021153288904	26.13943784410316	25.50935960591133
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	143.0
1	537.0
2	931.0
3	1535.5
4	2140.0
5	1554.0
6	968.0
7	963.5
8	959.0
9	923.5
10	888.0
11	901.0
12	914.0
13	945.5
14	977.0
15	943.0
16	909.0
17	886.5
18	864.0
19	891.5
20	919.0
21	1049.5
22	1180.0
23	1335.0
24	1490.0
25	1850.0
26	2644.0
27	3078.0
28	4067.0
29	5056.0
30	5476.0
31	5896.0
32	7026.5
33	8157.0
34	9308.5
35	10460.0
36	10536.5
37	10613.0
38	12332.0
39	14051.0
40	16288.0
41	18525.0
42	20183.0
43	21841.0
44	24727.5
45	27614.0
46	34558.0
47	41502.0
48	38537.0
49	35572.0
50	34550.0
51	33528.0
52	30616.0
53	27704.0
54	26091.0
55	24478.0
56	23959.5
57	23441.0
58	22674.5
59	21908.0
60	21046.0
61	20184.0
62	18781.5
63	17379.0
64	15567.5
65	13756.0
66	11945.0
67	10134.0
68	8755.5
69	7377.0
70	6358.5
71	5340.0
72	4447.5
73	3555.0
74	2768.0
75	1675.5
76	1370.0
77	1030.5
78	691.0
79	540.5
80	390.0
81	293.0
82	196.0
83	126.0
84	56.0
85	33.0
86	10.0
87	11.5
88	13.0
89	11.5
90	10.0
91	9.0
92	8.0
93	5.5
94	3.0
95	4.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	431375.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.802756116613576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.11273171233246	20.770454244130974
2	7.647402870410745	4.405325596038627
3	2.8761378646624554	2.485220924208914
4	1.7662439171807327	2.034907711560355
5	1.3410125011387746	1.9312428009815057
6	1.1606542644692082	2.0058025029128475
7	1.053066104997834	2.1231844337847363
8	0.9400379094534891	2.1660546117088098
9	0.8770862759927729	2.27362518905838
>10	10.098945987857485	53.24781947741464
>50	0.07826762659804208	1.537355570229505
>100	0.04276478566697144	2.6454600840396756
>500	0.00242064824530027	0.4657385582373285
>1k	0.0032275309937336936	1.9078082956937272
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG	2512	0.582323964068386	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	2417	0.56030136192408	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC	2219	0.5144016227180528	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1061	0.24595769342219648	No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	907	0.21025789626195304	Illumina Single End Adapter 2 (95% over 21bp)
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	581	0.13468559837728195	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCT	516	0.11961750217328311	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	485	0.11243117936829905	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	433	0.10037670240509997	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.636337293538105E-4	0.0	0.0	0.09411764705882353	0.0
2	4.636337293538105E-4	0.0	0.0	0.5072152999130687	0.0
3	4.636337293538105E-4	0.0	0.0	0.6627644161112721	0.0
4	4.636337293538105E-4	0.0	0.0	1.0088669950738915	0.0
5	4.636337293538105E-4	0.0	0.0	2.24792813677195	0.0
6	4.636337293538105E-4	0.0	0.0	2.8001159084323386	0.0
7	4.636337293538105E-4	0.0	0.0	3.3383946682121124	0.0
8	4.636337293538105E-4	0.0	0.0	4.0452042886119965	0.0
9	4.636337293538105E-4	0.0	0.0	4.341929875398435	0.0
10	4.636337293538105E-4	0.0	0.0	5.498696030136192	0.0
11	4.636337293538105E-4	0.0	0.0	6.237264560996812	0.0
12	4.636337293538105E-4	0.0	0.0	7.350912778904665	0.0
13	4.636337293538105E-4	0.0	0.0	7.6290930165169515	0.0
14	4.636337293538105E-4	0.0	0.0	7.745001448855405	0.0
15	4.636337293538105E-4	0.0	0.0	8.020631700956244	0.0
16	4.636337293538105E-4	0.0	0.0	8.42329759490003	0.0
17	4.636337293538105E-4	0.0	0.0	8.882526803824979	0.0
18	4.636337293538105E-4	0.0	0.0	9.325297015357867	0.0
19	4.636337293538105E-4	0.0	0.0	9.705244856563315	0.0
20	4.636337293538105E-4	0.0	0.0	10.004752245725877	0.0
21	4.636337293538105E-4	0.0	0.0	10.34227760069545	0.0
22	4.636337293538105E-4	0.0	0.0	10.725702694871051	0.0
23	4.636337293538105E-4	0.0	0.0	11.075514343668502	0.0
24	4.636337293538105E-4	0.0	0.0	11.392639814546508	0.0
25	4.636337293538105E-4	0.0	0.0	11.682179078527962	0.0
26	4.636337293538105E-4	0.0	0.0	11.945986670530282	0.0
27	4.636337293538105E-4	0.0	0.0	12.213503332367429	0.0
28	4.636337293538105E-4	0.0	0.0	12.489365401332947	0.0
29	4.636337293538105E-4	0.0	0.0	12.762213851057664	0.0
30	4.636337293538105E-4	0.0	0.0	13.103680092726746	0.0
31	4.636337293538105E-4	0.0	0.0	13.378151260504202	0.0
32	6.954505940307157E-4	0.0	0.0	13.686004056795133	0.0
33	6.954505940307157E-4	0.0	0.0	13.97531150391191	0.0
34	6.954505940307157E-4	0.0	0.0	14.26971892205158	0.0
35	9.27267458707621E-4	0.0	0.0	14.572471747319618	0.0
36	9.27267458707621E-4	0.0	0.0	14.86664734859461	0.0
37	9.27267458707621E-4	0.0	0.0	15.16035931614025	0.0
38	9.27267458707621E-4	0.0	0.0	15.445494059692843	0.0
39	9.27267458707621E-4	0.0	0.0	15.73642422486236	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATGG	30	2.1623364E-6	45.000004	2
AAGTACG	25	3.8867394E-5	45.0	1
CGAACTA	20	7.028382E-4	45.0	19
ATAGCGG	20	7.028382E-4	45.0	2
GCGATAT	35	1.2095734E-7	45.0	9
ATAACGG	25	3.8867394E-5	45.0	2
TGTAACG	20	7.028382E-4	45.0	14
GTAACGA	20	7.028382E-4	45.0	15
TGCGTAG	35	1.2095734E-7	45.0	1
CGGTCTA	40	3.4515324E-7	39.375	31
ATGTAGG	110	0.0	38.863636	2
CAACGTT	35	6.2392173E-6	38.571426	18
ACTTACG	35	6.2392173E-6	38.571426	1
GTAACGG	35	6.2392173E-6	38.571426	2
CGGGACG	65	9.094947E-12	38.076927	6
TACGGCT	190	0.0	37.894737	7
CCGATAG	30	1.1387396E-4	37.500004	11
ATTCCCG	30	1.1387396E-4	37.500004	12
TCTACGG	30	1.1387396E-4	37.500004	2
GGTATTC	30	1.1387396E-4	37.500004	8
>>END_MODULE