FastQCFastQC Report
Sat 18 Jun 2016
SRR3550552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775700
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48060.6195694211679773No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT207.031867E-445.04
GCGATAT253.8896284E-545.09
ACGGGTC700.041.7857135
CGTTTTT25900.041.1776051
CGACGGT451.9268555E-840.028
CATAGCG356.245664E-638.5714261
TCGCAGG659.094947E-1238.076922
CGTTGTA301.1395795E-437.50000427
ATAGACG301.1395795E-437.5000041
TAACGCC301.1395795E-437.50000412
ATAGGGC1950.036.9230774
CTAACGG552.746674E-936.8181842
CACGACC1600.036.562527
ACACGAC1550.036.29032526
ACGACCA1550.036.29032528
AACGTAG250.00210668736.01
TTAGGCG504.8754373E-836.01
TAATACG250.00210668736.01
CCGCTCG250.00210668736.019
ACGGGCT1750.036.05
Produced by FastQC (version 0.11.5)