##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550552_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	775700
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29968286708779	33.0	31.0	34.0	31.0	34.0
2	32.3210416398092	34.0	31.0	34.0	31.0	34.0
3	32.29967384298053	34.0	31.0	34.0	30.0	34.0
4	35.93560654892355	37.0	35.0	37.0	35.0	37.0
5	36.014268402733016	37.0	35.0	37.0	35.0	37.0
6	35.970846976924065	37.0	35.0	37.0	35.0	37.0
7	36.23456233079799	37.0	36.0	37.0	35.0	37.0
8	36.078218383395644	37.0	37.0	37.0	35.0	37.0
9	37.94705814103391	39.0	38.0	39.0	35.0	39.0
10	37.56611705556272	39.0	37.0	39.0	35.0	39.0
11	37.53560010313265	39.0	37.0	39.0	35.0	39.0
12	37.521924713162306	39.0	37.0	39.0	35.0	39.0
13	37.545512440376434	39.0	37.0	39.0	35.0	39.0
14	38.754579089854325	40.0	38.0	41.0	35.0	41.0
15	38.87110223024365	40.0	38.0	41.0	35.0	41.0
16	38.644131751965965	40.0	38.0	41.0	35.0	41.0
17	38.71075673585149	40.0	38.0	41.0	35.0	41.0
18	38.59107773623823	40.0	38.0	41.0	35.0	41.0
19	38.42657599587469	40.0	37.0	41.0	34.0	41.0
20	38.577765888874566	40.0	38.0	41.0	35.0	41.0
21	38.48077736238236	40.0	38.0	41.0	35.0	41.0
22	38.575080572386234	40.0	38.0	41.0	35.0	41.0
23	38.5823952558979	40.0	38.0	41.0	35.0	41.0
24	38.51479953590306	40.0	38.0	41.0	35.0	41.0
25	38.26506639164626	40.0	37.0	41.0	34.0	41.0
26	38.368530359675134	40.0	37.0	41.0	34.0	41.0
27	38.38774655150187	40.0	37.0	41.0	35.0	41.0
28	38.31157921877014	40.0	37.0	41.0	34.0	41.0
29	38.2944347041382	40.0	37.0	41.0	34.0	41.0
30	38.12467835503416	40.0	37.0	41.0	34.0	41.0
31	38.075342271496716	40.0	37.0	41.0	34.0	41.0
32	38.00986592754931	40.0	37.0	41.0	34.0	41.0
33	37.90111125435091	40.0	37.0	41.0	34.0	41.0
34	37.77846461260797	40.0	37.0	41.0	33.0	41.0
35	37.66319066649478	40.0	37.0	41.0	33.0	41.0
36	37.53669588758541	40.0	36.0	41.0	33.0	41.0
37	37.51238365347428	40.0	36.0	41.0	33.0	41.0
38	37.39282583472992	40.0	36.0	41.0	33.0	41.0
39	37.33663529715096	40.0	36.0	41.0	33.0	41.0
40	37.25540157277298	40.0	36.0	41.0	33.0	41.0
41	37.11154183318293	40.0	35.0	41.0	32.0	41.0
42	37.09411112543509	40.0	35.0	41.0	32.0	41.0
43	37.039850457651156	40.0	35.0	41.0	32.0	41.0
44	36.929122083279616	40.0	35.0	41.0	32.0	41.0
45	36.904624210390615	40.0	35.0	41.0	32.0	41.0
46	36.79056722959907	39.0	35.0	41.0	31.0	41.0
47	36.74288255768983	39.0	35.0	41.0	31.0	41.0
48	36.62978857805853	39.0	35.0	41.0	31.0	41.0
49	36.56636973056594	39.0	35.0	41.0	31.0	41.0
50	36.47438700528555	39.0	35.0	41.0	31.0	41.0
51	35.44145674874307	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	23.0
10	17.0
11	20.0
12	18.0
13	19.0
14	25.0
15	26.0
16	48.0
17	87.0
18	148.0
19	232.0
20	470.0
21	734.0
22	1158.0
23	1755.0
24	2684.0
25	4210.0
26	6084.0
27	7400.0
28	7778.0
29	8010.0
30	9197.0
31	10810.0
32	13975.0
33	19442.0
34	37450.0
35	51468.0
36	49781.0
37	76846.0
38	150255.0
39	315311.0
40	214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.73249967771046	20.945984272270206	24.37089080830218	12.950625241717159
2	34.213097847105836	23.69047312105195	25.317519659662242	16.778909372179967
3	32.112672424906535	23.389841433543896	28.303854582957328	16.19363155859224
4	28.466288513600617	25.64509475312621	27.490524687379143	18.398092045894032
5	25.339306432899317	29.82415882428774	25.75738043057883	19.079154312234113
6	26.236818357612478	35.33647028490396	24.26853164883331	14.15817970865025
7	81.9035709681578	5.418976408405311	8.69446951140905	3.9829831120278456
8	83.63658630914013	4.561041639809204	8.289802758798505	3.5125692922521594
9	76.97795539512698	7.718963516823514	10.223024365089596	5.080056722959907
10	36.44243908727601	32.918525203042414	16.756606935671005	13.882428774010572
11	27.75235271367797	24.75609127239912	28.92961196338791	18.561944050535
12	26.183318293154574	23.02797473249968	31.302307593141677	19.486399381204073
13	23.900992651798376	23.436380043831377	32.71870568518757	19.94392161918267
14	20.56890550470543	27.6889261312363	30.7942503545185	20.94791800953977
15	20.140776073224185	27.04215547247647	33.14038932577027	19.67667912852907
16	23.720252675003223	25.24880752868377	31.86038416913755	19.170555627175453
17	23.072708521335567	25.20768338275106	29.386489622276653	22.333118473636716
18	23.90911434833054	25.455330669073096	30.012504834343172	20.623050148253192
19	24.144127884491425	27.00438313781101	28.66314296764213	20.188346010055433
20	26.01095784452752	25.925486657212836	28.43509088565167	19.628464612607967
21	25.247260538868122	26.12053628980276	29.085729018950623	19.546474152378497
22	24.17274719608096	24.398865540801857	28.807270852133556	22.62111641098363
23	22.510635554982596	26.457135490524685	29.18602552533196	21.846203429160756
24	22.11486399381204	26.024107257960548	30.468866829960035	21.392161918267373
25	23.11628206780972	26.61802243135233	28.432254737656308	21.833440763181642
26	21.952816810622664	27.27961840917881	28.887843238365345	21.879721541833185
27	22.228825576898288	26.651669459842726	29.432383653474282	21.68712130978471
28	20.818486528297022	27.32216062910919	30.276653345365478	21.58269949722831
29	21.89390228180998	25.993554209101454	30.002320484723477	22.110223024365087
30	22.30076060332603	25.079798891323968	30.80301663014052	21.816423875209487
31	23.039190408663142	26.17738816552791	28.933608353745004	21.84981307206394
32	22.72141291736496	26.18667010442181	29.386618538094623	21.705298440118604
33	22.440892097460356	26.366120923037258	28.68080443470414	22.512182544798247
34	21.769240685832152	26.332216062910916	29.50238494263246	22.396158308624468
35	21.756220188217092	26.27136779682867	28.984401186025526	22.988010828928708
36	21.867990202397834	28.045765115379655	28.579605517597006	21.5066391646255
37	22.617506768080446	28.282454557174162	28.101456748743068	20.99858192600232
38	21.191053242232822	28.029134974861414	27.83202268918396	22.9477890937218
39	22.616088694082766	26.389841433543896	27.68222250870182	23.31184736367152
40	22.754802114219412	25.698208070130207	29.71483821064845	21.832151605001933
41	21.048085600103132	27.70942374629367	28.885780585277814	22.356710068325384
42	21.531133170039965	28.109062782003353	28.67474539125951	21.685058656697176
43	22.136264019595203	26.485110223024368	28.930901121567615	22.44772463581281
44	21.81797086502514	26.589532035580767	28.52430063168751	23.068196467706585
45	22.142451978857807	25.353873920330024	28.26401959520433	24.239654505607838
46	22.058656697176744	26.315457006574704	28.47544153667655	23.150444759572
47	21.793476859610674	26.680675518886165	29.422199303854583	22.103648317648574
48	21.085213355678743	26.09681577929612	29.851102230243654	22.966868634781488
49	21.203558076575995	26.437282454557177	29.54389583601908	22.815263632847753
50	20.160758025009667	25.37669202011087	30.326028103648316	24.136521851231148
51	20.55756091272399	25.46809333505221	29.42916075802501	24.545184994198788
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	265.0
1	653.5
2	1042.0
3	4702.0
4	8362.0
5	5621.0
6	2880.0
7	2814.0
8	2748.0
9	2673.0
10	2598.0
11	2494.5
12	2391.0
13	2431.5
14	2472.0
15	2344.0
16	2216.0
17	2203.5
18	2191.0
19	2169.5
20	2148.0
21	2339.5
22	2531.0
23	3075.0
24	3619.0
25	4408.5
26	6407.5
27	7617.0
28	9293.5
29	10970.0
30	12979.0
31	14988.0
32	16402.5
33	17817.0
34	20439.0
35	23061.0
36	24857.5
37	26654.0
38	29362.5
39	32071.0
40	35539.5
41	39008.0
42	42436.5
43	45865.0
44	52499.0
45	59133.0
46	62802.0
47	66471.0
48	65903.5
49	65336.0
50	61104.0
51	56872.0
52	52024.5
53	47177.0
54	44270.0
55	41363.0
56	39968.5
57	38574.0
58	37046.0
59	35518.0
60	33802.5
61	32087.0
62	28690.5
63	25294.0
64	21516.0
65	17738.0
66	14737.5
67	11737.0
68	9474.5
69	7212.0
70	6040.5
71	4869.0
72	4036.5
73	3204.0
74	2538.5
75	1533.0
76	1193.0
77	916.0
78	639.0
79	519.5
80	400.0
81	261.0
82	122.0
83	110.0
84	98.0
85	72.0
86	46.0
87	31.0
88	16.0
89	13.5
90	11.0
91	7.5
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	775700.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.32822717174445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.87805710276965	20.787277602019035
2	7.906918396433181	4.637917979180754
3	3.242114286486577	2.8525639293250955
4	1.9913052087162226	2.336058061180295
5	1.539883726848685	2.258102987954536
6	1.2812074058340914	2.254532511145417
7	1.1122398556417523	2.28340162090107
8	0.9853567460868891	2.311901319555776
9	0.9311126055265337	2.4577093815621356
>10	10.01953986516158	53.37419649069992
>50	0.07469587124962426	1.4820262891170988
>100	0.035800979633765086	2.1063102537499745
>500	0.0013259622086579663	0.2350138771646688
>1k	4.4198740288598875E-4	0.6229876964442351
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4806	0.6195694211679773	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.1566327188346E-4	0.0	0.0	0.07915431223411112	0.0
2	5.1566327188346E-4	0.0	0.0	0.2849039577156117	0.0
3	5.1566327188346E-4	0.0	0.0	0.4545571741652701	0.0
4	5.1566327188346E-4	0.0	0.0	0.7060719350264277	0.0
5	5.1566327188346E-4	0.0	0.0	1.3833956426453526	0.0
6	5.1566327188346E-4	0.0	0.0	2.0558205491813846	0.0
7	5.1566327188346E-4	0.0	0.0	2.579863349232951	0.0
8	5.1566327188346E-4	0.0	0.0	3.3995101198917106	0.0
9	5.1566327188346E-4	0.0	0.0	3.825319066649478	0.0
10	5.1566327188346E-4	0.0	0.0	4.507799406987237	0.0
11	5.1566327188346E-4	0.0	0.0	5.287740105710971	0.0
12	5.1566327188346E-4	0.0	0.0	5.912595075415753	0.0
13	5.1566327188346E-4	0.0	0.0	6.186670104421813	0.0
14	5.1566327188346E-4	0.0	0.0	6.317390743844269	0.0
15	5.1566327188346E-4	0.0	0.0	6.506381332989558	0.0
16	5.1566327188346E-4	0.0	0.0	6.856645610416398	0.0
17	5.1566327188346E-4	0.0	0.0	7.295475054789223	0.0
18	5.1566327188346E-4	0.0	0.0	7.794121438700529	0.0
19	5.1566327188346E-4	0.0	0.0	8.085342271496712	0.0
20	6.445790898543251E-4	0.0	0.0	8.372953461389713	0.0
21	6.445790898543251E-4	0.0	0.0	8.746422586051308	0.0
22	6.445790898543251E-4	0.0	0.0	9.156761634652572	0.0
23	6.445790898543251E-4	0.0	0.0	9.569163336341369	0.0
24	6.445790898543251E-4	0.0	0.0	9.894804692535773	0.0
25	6.445790898543251E-4	0.0	0.0	10.178032744617765	0.0
26	6.445790898543251E-4	0.0	0.0	10.456490911434834	0.0
27	6.445790898543251E-4	0.0	0.0	10.74229727987624	0.0
28	6.445790898543251E-4	0.0	0.0	11.037643418847493	0.0
29	6.445790898543251E-4	0.0	0.0	11.348201624339307	0.0
30	6.445790898543251E-4	0.0	0.0	11.74371535387392	0.0
31	6.445790898543251E-4	0.0	0.0	12.083150702591208	0.0
32	7.734949078251901E-4	0.0	0.0	12.407760732241846	0.0
33	7.734949078251901E-4	0.0	0.0	12.732628593528426	0.0
34	7.734949078251901E-4	0.0	0.0	13.054402475183705	0.0
35	7.734949078251901E-4	0.0	0.0	13.410339048601264	0.0
36	7.734949078251901E-4	0.0	0.0	13.744488848781746	0.0
37	7.734949078251901E-4	0.0	0.0	14.082506123501354	0.0
38	7.734949078251901E-4	0.0	0.0	14.430836663658631	0.0
39	7.734949078251901E-4	0.0	0.0	14.798891323965451	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	20	7.031867E-4	45.0	4
GCGATAT	25	3.8896284E-5	45.0	9
ACGGGTC	70	0.0	41.785713	5
CGTTTTT	2590	0.0	41.177605	1
CGACGGT	45	1.9268555E-8	40.0	28
CATAGCG	35	6.245664E-6	38.571426	1
TCGCAGG	65	9.094947E-12	38.07692	2
CGTTGTA	30	1.1395795E-4	37.500004	27
ATAGACG	30	1.1395795E-4	37.500004	1
TAACGCC	30	1.1395795E-4	37.500004	12
ATAGGGC	195	0.0	36.923077	4
CTAACGG	55	2.746674E-9	36.818184	2
CACGACC	160	0.0	36.5625	27
ACACGAC	155	0.0	36.290325	26
ACGACCA	155	0.0	36.290325	28
AACGTAG	25	0.002106687	36.0	1
TTAGGCG	50	4.8754373E-8	36.0	1
TAATACG	25	0.002106687	36.0	1
CCGCTCG	25	0.002106687	36.0	19
ACGGGCT	175	0.0	36.0	5
>>END_MODULE