FastQCFastQC Report
Sat 18 Jun 2016
SRR3550551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325049
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC8080.24857790671560287TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC7630.23473384012871906TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT6110.18797165965746704TruSeq Adapter, Index 22 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.1495159191383453No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3850.11844368079889493No Hit
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC3830.117828388950589TruSeq Adapter, Index 20 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC3620.11136782454337654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA302.1606793E-645.00000424
TCGTTAG207.0258114E-445.0000041
CCGATGA253.8846105E-545.00000418
GAGTACG207.0258114E-445.0000041
GCCATTA207.0258114E-445.00000410
CGTTACT207.0258114E-445.00000429
TAGCAGT207.0258114E-445.00000414
TTAAGCG351.2083365E-745.0000041
TGCTCGC207.0258114E-445.00000423
CACGTCG253.8846105E-545.00000441
AATAGCG207.0258114E-445.0000041
AACTAAC351.2083365E-745.00000413
TCAACGG207.0258114E-445.0000042
TGCGTAG207.0258114E-445.0000041
TTGCGAG453.8380676E-1045.01
ACAACGA900.042.513
CACAACG900.042.512
ACACAAC1000.040.50000411
CAACGAG950.040.26315314
TACGGGA900.040.04