Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550551_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 325049 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC | 808 | 0.24857790671560287 | TruSeq Adapter, Index 20 (95% over 21bp) |
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC | 763 | 0.23473384012871906 | TruSeq Adapter, Index 20 (95% over 21bp) |
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT | 611 | 0.18797165965746704 | TruSeq Adapter, Index 22 (95% over 23bp) |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 486 | 0.1495159191383453 | No Hit |
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 385 | 0.11844368079889493 | No Hit |
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC | 383 | 0.117828388950589 | TruSeq Adapter, Index 20 (95% over 21bp) |
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC | 362 | 0.11136782454337654 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCACGA | 30 | 2.1606793E-6 | 45.000004 | 24 |
TCGTTAG | 20 | 7.0258114E-4 | 45.000004 | 1 |
CCGATGA | 25 | 3.8846105E-5 | 45.000004 | 18 |
GAGTACG | 20 | 7.0258114E-4 | 45.000004 | 1 |
GCCATTA | 20 | 7.0258114E-4 | 45.000004 | 10 |
CGTTACT | 20 | 7.0258114E-4 | 45.000004 | 29 |
TAGCAGT | 20 | 7.0258114E-4 | 45.000004 | 14 |
TTAAGCG | 35 | 1.2083365E-7 | 45.000004 | 1 |
TGCTCGC | 20 | 7.0258114E-4 | 45.000004 | 23 |
CACGTCG | 25 | 3.8846105E-5 | 45.000004 | 41 |
AATAGCG | 20 | 7.0258114E-4 | 45.000004 | 1 |
AACTAAC | 35 | 1.2083365E-7 | 45.000004 | 13 |
TCAACGG | 20 | 7.0258114E-4 | 45.000004 | 2 |
TGCGTAG | 20 | 7.0258114E-4 | 45.000004 | 1 |
TTGCGAG | 45 | 3.8380676E-10 | 45.0 | 1 |
ACAACGA | 90 | 0.0 | 42.5 | 13 |
CACAACG | 90 | 0.0 | 42.5 | 12 |
ACACAAC | 100 | 0.0 | 40.500004 | 11 |
CAACGAG | 95 | 0.0 | 40.263153 | 14 |
TACGGGA | 90 | 0.0 | 40.0 | 4 |