FastQCFastQC Report
Sat 18 Jun 2016
SRR3550546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811258
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74950.9238737861444819No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTT9680.1193208572365388No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA9430.11623922352691746No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8430.10391268868843204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT40950.042.527471
CGACGGT603.6379788E-1241.24999628
CGGTCTA603.6379788E-1241.24999631
GTACGAG451.9270374E-840.01
ACACGAC800.039.37526
CGAATAT1150.039.13043614
TAGCCGT356.2460167E-638.5714344
CCCCGAT356.2460167E-638.5714340
TATAGCG356.2460167E-638.571431
TAATGCG356.2460167E-638.571431
GAGTACG301.1396261E-437.4999961
GCCGATA601.546141E-1037.4999969
GCGCGAC2350.037.3404279
GACACGA850.037.05882325
TACGCGG552.746674E-936.818182
TTAACGG552.746674E-936.818182
CGCGACC2400.036.562510
ACGGGAC3350.036.2686545
GTATTCG250.002106743636.0000049
CGACCCG2000.036.00000432
Produced by FastQC (version 0.11.5)