##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550546_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	811258
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.210646921201395	33.0	31.0	34.0	30.0	34.0
2	32.24340345488118	34.0	31.0	34.0	30.0	34.0
3	32.20424451900628	34.0	31.0	34.0	30.0	34.0
4	35.873918038404554	37.0	35.0	37.0	35.0	37.0
5	35.95336748605253	37.0	35.0	37.0	35.0	37.0
6	35.903737158832335	37.0	35.0	37.0	35.0	37.0
7	36.16323290494516	37.0	35.0	37.0	35.0	37.0
8	35.995420692307505	37.0	35.0	37.0	35.0	37.0
9	37.87317105039334	39.0	38.0	39.0	35.0	39.0
10	37.48997359656238	39.0	37.0	39.0	35.0	39.0
11	37.47414879113673	39.0	37.0	39.0	35.0	39.0
12	37.42123220972859	39.0	37.0	39.0	35.0	39.0
13	37.4252617539673	39.0	37.0	39.0	35.0	39.0
14	38.639940684714354	40.0	38.0	41.0	35.0	41.0
15	38.75313648678965	40.0	38.0	41.0	35.0	41.0
16	38.500501689967926	40.0	38.0	41.0	34.0	41.0
17	38.557817611659914	40.0	38.0	41.0	34.0	41.0
18	38.42693076678442	40.0	38.0	41.0	34.0	41.0
19	38.2382965714976	40.0	37.0	41.0	34.0	41.0
20	38.353759223329696	40.0	37.0	41.0	34.0	41.0
21	38.256632292069845	40.0	37.0	41.0	34.0	41.0
22	38.35363102736737	40.0	37.0	41.0	34.0	41.0
23	38.36248764264882	40.0	37.0	41.0	34.0	41.0
24	38.28865293161978	40.0	37.0	41.0	34.0	41.0
25	38.029694622425914	40.0	36.0	41.0	34.0	41.0
26	38.12377936488762	40.0	36.0	41.0	34.0	41.0
27	38.140192146025065	40.0	36.0	41.0	34.0	41.0
28	38.040454454686426	40.0	36.0	41.0	34.0	41.0
29	38.02024510081873	40.0	36.0	41.0	34.0	41.0
30	37.83636525001911	40.0	36.0	41.0	33.0	41.0
31	37.7649699602346	40.0	36.0	41.0	33.0	41.0
32	37.65379940783327	40.0	36.0	41.0	33.0	41.0
33	37.50377315231406	40.0	36.0	41.0	33.0	41.0
34	37.33242692213821	40.0	36.0	41.0	33.0	41.0
35	37.209427826905866	40.0	36.0	41.0	32.0	41.0
36	37.07411575602336	40.0	35.0	41.0	32.0	41.0
37	37.02063092135917	40.0	35.0	41.0	32.0	41.0
38	36.89210460790525	40.0	35.0	41.0	31.0	41.0
39	36.82122703258396	40.0	35.0	41.0	31.0	41.0
40	36.73625776263531	40.0	35.0	41.0	31.0	41.0
41	36.59122005576524	39.0	35.0	41.0	31.0	41.0
42	36.56096211069721	39.0	35.0	41.0	31.0	41.0
43	36.49250916477865	39.0	35.0	41.0	30.0	41.0
44	36.362189340505736	39.0	35.0	41.0	30.0	41.0
45	36.339641642979174	39.0	35.0	41.0	30.0	41.0
46	36.23118292824231	39.0	35.0	41.0	30.0	41.0
47	36.178547391828495	39.0	35.0	41.0	30.0	41.0
48	36.058067840317136	39.0	35.0	41.0	29.0	41.0
49	36.005841544859955	39.0	35.0	41.0	29.0	41.0
50	35.90345857914498	39.0	35.0	41.0	29.0	41.0
51	34.87208631532755	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	15.0
10	15.0
11	12.0
12	16.0
13	17.0
14	24.0
15	37.0
16	60.0
17	123.0
18	209.0
19	361.0
20	657.0
21	994.0
22	1425.0
23	2124.0
24	3451.0
25	5548.0
26	8133.0
27	10222.0
28	10440.0
29	10631.0
30	11168.0
31	13382.0
32	16518.0
33	22406.0
34	42796.0
35	57442.0
36	54087.0
37	81597.0
38	153269.0
39	303877.0
40	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.985060239775756	20.98580229717303	24.264907094906924	14.76423036814429
2	35.11842102019333	23.512495408365773	24.51365656794756	16.85542700349334
3	31.727268020777604	23.20556469088749	29.00125484124656	16.065912447088348
4	28.40403422832194	24.906996294643626	28.210507631357718	18.478461845676712
5	25.116670652246263	29.668736702750543	26.886884320401155	18.32770832460204
6	25.71031657006772	34.14277578772721	26.248000019722458	13.898907622482614
7	79.13980509283114	5.968261638097868	10.771419203262095	4.120514065808904
8	80.94736816154663	5.295356101264949	10.1072655061645	3.650010231023916
9	74.46274797906462	7.986978248596624	12.223731537932446	5.326542234406317
10	37.75272478052605	29.616965256428905	18.66582517522169	13.964484787823356
11	29.032440974387924	24.725303171124352	28.057042272618578	18.185213581869146
12	26.55887522834906	22.462028109430047	31.210539680348298	19.7685569818726
13	23.35594841591701	23.27829124643455	32.840354116692836	20.525406220955603
14	21.10610927719665	26.586116870342106	31.76054966484152	20.547224187619722
15	21.101671724654793	25.707234936358102	33.732302177605646	19.45879116138146
16	23.481802336617942	24.62792354590032	32.36442660657892	19.525847510902818
17	23.243900214235175	24.80320687130358	30.29923895973907	21.65365395472217
18	23.55810358726817	25.237100897618266	30.602348451417427	20.602447063696136
19	24.38928183142724	26.253300429703003	28.766064556528253	20.5913531823415
20	25.610841433921145	25.774661081924616	29.017525867233356	19.59697161692088
21	24.671559479228556	26.199310207110436	29.420973352496986	19.70815696116402
22	23.56069215958425	24.462008386974304	29.605131782983957	22.372167670457486
23	23.22553367732583	25.760732097557128	29.811354710831818	21.202379514285223
24	22.807171084907637	25.190876391973948	30.888324059670293	21.113628463448126
25	23.536408885952433	25.970159924462994	28.8747845938037	21.618646595780874
26	22.043049190269926	26.97612843263179	28.920146242009327	22.06067613508896
27	22.43860769323692	26.243932263225755	29.249018191500113	22.068441852037207
28	21.270052190548505	27.18013258420872	29.90096861910761	21.648846606135162
29	22.550286098873602	25.965845637269524	29.31360923405378	22.1702590298031
30	22.744429022579745	25.274573563527262	29.52550236792734	22.455495045965648
31	23.337335348310894	26.28954044212815	27.722130321056927	22.650993888504026
32	22.78744862916606	26.247630223677298	28.637622063511238	22.3272990836454
33	22.65087062315564	26.43129559277073	28.39207748952861	22.525756294545015
34	20.92934676761277	26.474561730053818	29.252346355906507	23.343745146426908
35	21.674239267902443	25.78107088004063	28.61728328102774	23.92740657102919
36	21.99189407069021	27.827522193925976	27.775627482255956	22.404956253127857
37	22.63225755554953	27.488049424474088	27.814579332345566	22.065113687630816
38	22.010507138296326	26.973786391012478	28.0633288053862	22.952377665304997
39	23.327720651136875	25.44394015220805	27.79362422312014	23.434714973534927
40	22.48187383052001	25.088073091420977	29.5315423699982	22.898510708060815
41	20.81051897176977	26.743156924184415	28.324774609310477	24.121549494735337
42	21.942957727381422	27.27652608664568	27.86782996284782	22.91268622312507
43	22.519346496429	25.37219971944807	28.610626952214957	23.497826831907975
44	21.760525011771843	25.807326399246598	29.00002218776271	23.43212640121885
45	22.055498990456794	25.517652830542193	28.46381792228859	23.963030256712415
46	21.904375673336965	26.49576336997601	28.051865127986414	23.54799582870061
47	22.274541514536683	25.34286256653247	28.956016458389318	23.42657946054153
48	21.435351022732597	25.723629227693284	29.186399394520606	23.65462035505351
49	21.707520911966355	25.521967117735667	29.217092466268436	23.553419504029545
50	20.281587361850363	25.039753074854115	30.351010406060713	24.327649157234813
51	20.758624260099744	24.775348902568602	29.536596249281978	24.92943058804967
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	277.0
1	746.0
2	1215.0
3	7157.5
4	13100.0
5	8730.5
6	4361.0
7	4191.0
8	4021.0
9	3937.5
10	3854.0
11	3758.0
12	3662.0
13	3507.0
14	3352.0
15	3144.0
16	2936.0
17	2900.5
18	2865.0
19	2917.5
20	2970.0
21	3028.0
22	3086.0
23	3578.5
24	4071.0
25	4550.0
26	5895.5
27	6762.0
28	8644.0
29	10526.0
30	11939.0
31	13352.0
32	15416.0
33	17480.0
34	19833.5
35	22187.0
36	24069.5
37	25952.0
38	28184.5
39	30417.0
40	33654.5
41	36892.0
42	38849.5
43	40807.0
44	44378.0
45	47949.0
46	50961.0
47	53973.0
48	58499.0
49	63025.0
50	65087.5
51	67150.0
52	64482.5
53	61815.0
54	55672.0
55	49529.0
56	46953.5
57	44378.0
58	41374.0
59	38370.0
60	37612.5
61	36855.0
62	33176.0
63	29497.0
64	25280.5
65	21064.0
66	17364.5
67	13665.0
68	11379.0
69	9093.0
70	7661.0
71	6229.0
72	5075.5
73	3922.0
74	3192.0
75	2067.0
76	1672.0
77	1188.0
78	704.0
79	568.5
80	433.0
81	301.0
82	169.0
83	119.5
84	70.0
85	49.0
86	28.0
87	23.0
88	18.0
89	11.0
90	4.0
91	4.0
92	4.0
93	4.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	811258.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.450376980271344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.35427812951993	21.423142912187416
2	8.071051121572529	4.915330985578507
3	3.333588582780192	3.04527087128356
4	2.17546486779444	2.6497490132670745
5	1.729012370444678	2.6324539241796505
6	1.4538506990792517	2.656218111599655
7	1.3083200616206074	2.788718736103953
8	1.1479021549419453	2.7963242683558467
9	1.0593979430915057	2.9033160065334362
>10	9.26481400161165	49.0013017343685
>50	0.06277805008320828	1.3221178139819598
>100	0.03505787115015259	2.0566000820221872
>500	0.004076496645366571	0.8790947477596034
>1k	0.0	0.0
>5k	4.076496645366571E-4	0.9303607927786258
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7495	0.9238737861444819	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTT	968	0.1193208572365388	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	943	0.11623922352691746	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	843	0.10391268868843204	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.163267419242707E-4	0.0	0.0	0.07519186251476102	0.0
2	6.163267419242707E-4	0.0	0.0	0.2668694792532092	0.0
3	6.163267419242707E-4	0.0	0.0	0.43611280258561397	0.0
4	6.163267419242707E-4	0.0	0.0	0.6809177844779343	0.0
5	6.163267419242707E-4	0.0	0.0	1.3253490258339518	0.0
6	6.163267419242707E-4	0.0	0.0	2.0693786686849314	0.0
7	7.395920903091248E-4	0.0	0.0	2.6423160079777332	0.0
8	7.395920903091248E-4	0.0	0.0	3.4677007807627165	0.0
9	7.395920903091248E-4	0.0	0.0	3.940793187863787	0.0
10	7.395920903091248E-4	0.0	0.0	4.621710972341721	0.0
11	7.395920903091248E-4	0.0	0.0	5.338992034593187	0.0
12	7.395920903091248E-4	0.0	0.0	5.902931003453895	0.0
13	7.395920903091248E-4	0.0	0.0	6.169307421313565	0.0
14	7.395920903091248E-4	0.0	0.0	6.302433997569207	0.0
15	7.395920903091248E-4	0.0	0.0	6.470567932766149	0.0
16	7.395920903091248E-4	0.0	0.0	6.772814567005811	0.0
17	7.395920903091248E-4	0.0	0.0	7.148527348882846	0.0
18	7.395920903091248E-4	0.0	0.0	7.576011577081521	0.0
19	7.395920903091248E-4	0.0	0.0	7.839676157276723	0.0
20	7.395920903091248E-4	0.0	0.0	8.118255844626495	0.0
21	7.395920903091248E-4	0.0	0.0	8.437389831594881	0.0
22	7.395920903091248E-4	0.0	0.0	8.78807974774979	0.0
23	7.395920903091248E-4	0.0	0.0	9.150603137349647	0.0
24	7.395920903091248E-4	0.0	0.0	9.438057929783128	0.0
25	7.395920903091248E-4	0.0	0.0	9.70579026647503	0.0
26	8.62857438693979E-4	0.0	0.0	9.95848423066398	0.0
27	8.62857438693979E-4	0.0	0.0	10.211178194852932	0.0
28	9.861227870788332E-4	0.0	0.0	10.486183187099542	0.0
29	9.861227870788332E-4	0.0	0.0	10.771912264655633	0.0
30	9.861227870788332E-4	0.0	0.0	11.103989113204431	0.0
31	0.0012326534838485413	0.0	0.0	11.383061861947741	0.0
32	0.0012326534838485413	0.0	0.0	11.676186860406924	0.0
33	0.0012326534838485413	0.0	0.0	11.97165390048542	0.0
34	0.0012326534838485413	0.0	0.0	12.244563381809487	0.0
35	0.0013559188322333955	0.0	0.0	12.554452467649009	0.0
36	0.0013559188322333955	0.0	0.0	12.850412569121044	0.0
37	0.0013559188322333955	0.0	0.0	13.163753084715344	0.0
38	0.0014791841806182497	0.0	0.0	13.473642170554866	0.0
39	0.0014791841806182497	0.0	0.0	13.794501872400643	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	4095	0.0	42.52747	1
CGACGGT	60	3.6379788E-12	41.249996	28
CGGTCTA	60	3.6379788E-12	41.249996	31
GTACGAG	45	1.9270374E-8	40.0	1
ACACGAC	80	0.0	39.375	26
CGAATAT	115	0.0	39.130436	14
TAGCCGT	35	6.2460167E-6	38.57143	44
CCCCGAT	35	6.2460167E-6	38.57143	40
TATAGCG	35	6.2460167E-6	38.57143	1
TAATGCG	35	6.2460167E-6	38.57143	1
GAGTACG	30	1.1396261E-4	37.499996	1
GCCGATA	60	1.546141E-10	37.499996	9
GCGCGAC	235	0.0	37.340427	9
GACACGA	85	0.0	37.058823	25
TACGCGG	55	2.746674E-9	36.81818	2
TTAACGG	55	2.746674E-9	36.81818	2
CGCGACC	240	0.0	36.5625	10
ACGGGAC	335	0.0	36.268654	5
GTATTCG	25	0.0021067436	36.000004	9
CGACCCG	200	0.0	36.000004	32
>>END_MODULE