##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550545_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595486
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09065536385406	33.0	31.0	34.0	30.0	34.0
2	32.1549994458308	33.0	31.0	34.0	30.0	34.0
3	32.02919799961712	33.0	31.0	34.0	30.0	34.0
4	35.77043121080932	37.0	35.0	37.0	35.0	37.0
5	35.88225247948734	37.0	35.0	37.0	35.0	37.0
6	35.83976617418378	37.0	35.0	37.0	35.0	37.0
7	36.11727731634329	37.0	35.0	37.0	35.0	37.0
8	35.96915124788828	37.0	35.0	37.0	35.0	37.0
9	37.849025837719104	39.0	38.0	39.0	35.0	39.0
10	37.44703317962135	39.0	37.0	39.0	34.0	39.0
11	37.4522759561098	39.0	37.0	39.0	35.0	39.0
12	37.49078063967919	39.0	37.0	39.0	35.0	39.0
13	37.52910731738446	39.0	37.0	39.0	35.0	39.0
14	38.75892128446345	40.0	38.0	41.0	35.0	41.0
15	38.85496216535737	40.0	38.0	41.0	35.0	41.0
16	38.6124694787115	40.0	38.0	41.0	34.0	41.0
17	38.663785546595555	40.0	38.0	41.0	35.0	41.0
18	38.42953822591967	40.0	38.0	41.0	34.0	41.0
19	38.13014915547973	40.0	37.0	41.0	34.0	41.0
20	38.15394484505093	40.0	36.0	41.0	34.0	41.0
21	38.05618939823942	40.0	36.0	41.0	34.0	41.0
22	38.169785687656805	40.0	36.0	41.0	34.0	41.0
23	38.16914083622453	40.0	36.0	41.0	34.0	41.0
24	38.09057307812442	40.0	36.0	41.0	34.0	41.0
25	37.8261588013824	40.0	35.0	41.0	34.0	41.0
26	37.92370769421951	40.0	35.0	41.0	34.0	41.0
27	37.94623887043524	40.0	35.0	41.0	34.0	41.0
28	37.86120748430694	40.0	35.0	41.0	34.0	41.0
29	37.81015506661785	40.0	35.0	41.0	34.0	41.0
30	37.57565081294942	40.0	35.0	41.0	33.0	41.0
31	37.425655682921175	40.0	35.0	41.0	33.0	41.0
32	37.14683636559046	40.0	35.0	41.0	32.0	41.0
33	36.794631611826304	40.0	35.0	41.0	31.0	41.0
34	36.47141494510366	40.0	35.0	41.0	30.0	41.0
35	36.23787293068183	40.0	35.0	41.0	28.0	41.0
36	36.0306018949228	40.0	35.0	41.0	26.0	41.0
37	35.95197536130153	40.0	35.0	41.0	25.0	41.0
38	35.800265665355695	40.0	35.0	41.0	24.0	41.0
39	35.71426700207898	39.0	35.0	41.0	24.0	41.0
40	35.64170778154314	39.0	35.0	41.0	23.0	41.0
41	35.501514729145605	39.0	35.0	41.0	23.0	41.0
42	35.45562112291473	39.0	35.0	41.0	23.0	41.0
43	35.388338264879444	39.0	35.0	41.0	23.0	41.0
44	35.26371568769039	39.0	35.0	41.0	22.0	41.0
45	35.262854206480085	39.0	35.0	41.0	23.0	41.0
46	35.12400627386706	39.0	35.0	41.0	22.0	41.0
47	35.08085496552396	39.0	35.0	41.0	22.0	41.0
48	34.94378709155211	39.0	35.0	41.0	21.0	41.0
49	34.87717763305938	39.0	35.0	41.0	20.0	41.0
50	34.772224703855336	39.0	35.0	41.0	20.0	41.0
51	33.72800703962814	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	8.0
10	7.0
11	16.0
12	2.0
13	8.0
14	10.0
15	23.0
16	47.0
17	102.0
18	192.0
19	361.0
20	638.0
21	961.0
22	1484.0
23	2498.0
24	4346.0
25	7822.0
26	11546.0
27	12318.0
28	11357.0
29	10214.0
30	9742.0
31	10670.0
32	12526.0
33	16170.0
34	26629.0
35	36959.0
36	38523.0
37	56637.0
38	111418.0
39	212090.0
40	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.912011365506494	19.330093402699646	23.85950299419298	20.898392237600884
2	38.8758090030664	22.76241590902221	23.20188887732037	15.159886210591015
3	27.565551499111653	21.946611675169525	35.456081251280466	15.031755574438357
4	24.73492239951905	23.656475551062496	34.75766012970918	16.850941919709282
5	22.82908414303611	27.43036780041848	32.69749414763739	17.043053908908018
6	23.019013041448495	31.57420997303043	31.522823374520982	13.883953611000091
7	73.87612807018134	4.919846982128883	16.896954756283105	4.307070191406683
8	76.03117453642906	4.322351826911127	15.889542323413144	3.7569313132466595
9	69.64009229436124	7.04113950621845	18.107898422465013	5.210869776955294
10	32.56650870045643	30.295590492471696	24.36530833638406	12.772592470687808
11	23.85614439298321	23.20222473744135	35.2817698484935	17.65986102108194
12	22.13704436376338	21.04986515216143	37.54832187490554	19.264768609169654
13	20.505100035937033	22.15904320168736	38.86119908780391	18.474657674571695
14	18.263905448658743	25.391528936028724	37.365446039033664	18.979119576278872
15	18.194046543495563	25.630325482043236	38.546498154448635	17.62912982001256
16	20.490825980795517	24.996053643578524	36.6870085946605	17.826111780965466
17	20.605018421927635	25.083041414911516	34.605011704725214	19.70692845843563
18	20.667992194610786	25.251979055762856	34.73767645251106	19.342352297115298
19	20.238091239760465	27.10475141313146	33.43756192420981	19.219595422898273
20	21.351971330980074	25.931256150438465	33.697013867664396	19.019758650917066
21	20.93181031963808	26.161320333307586	34.52423734563029	18.382632001424046
22	20.405853370188385	24.41333633368375	34.38451953530394	20.796290760823933
23	19.09515924807636	25.707909169988884	34.71030385265145	20.486627729283306
24	19.76990223111878	24.725014525950233	35.78203349868846	19.72304974424252
25	20.044132019896356	26.520186872571312	33.32387327325915	20.111807834273183
26	19.27870680419019	26.907937382239044	33.63773455631199	20.175621257258776
27	19.86427892511327	25.48674528032565	33.79306986226376	20.85590593229732
28	19.212710290418247	25.68809342285125	34.24681688570344	20.85237940102706
29	19.489123169982168	24.07143073052935	33.77879580712225	22.660650292366235
30	19.76083400785241	24.144648236902295	33.608682655847495	22.485835099397804
31	20.11382299499904	24.597387679979043	32.81840379118904	22.47038553383287
32	19.690471312507768	26.062409527679915	32.35945093587423	21.887668223938096
33	19.37392314848712	26.368881888071254	32.2497926063753	22.00740235706633
34	18.446277494349154	26.01051913898899	33.20162018922359	22.34158317743826
35	19.82078503944677	25.60597562327242	31.37588457159362	23.197354765687187
36	20.328437612303226	26.15325969040414	31.54818081365473	21.9701218836379
37	20.25253322496247	26.643783397090782	31.914436275579945	21.189247102366807
38	19.8421121571288	26.854031832822265	32.20680251089026	21.097053499158672
39	20.397121007042987	25.70807710004937	32.0860607973991	21.808741095508545
40	21.689510752561773	24.883036712869824	32.217717964822015	21.20973456974639
41	19.754452665553853	25.550390773250758	32.133249144396345	22.561907416799052
42	20.294851600205547	26.65520264120399	31.395700318731258	21.654245439859206
43	20.527266803921503	25.747540664264147	32.131233983670484	21.593958548143867
44	20.668495984792255	26.362668475833186	30.52632639558277	22.442509143791796
45	20.66278636273565	25.431496290424967	30.07912864450214	23.82658870233725
46	20.81341962699375	25.82546021233077	29.601703482533594	23.759416678141886
47	20.07116875963499	25.226285756508133	31.4645516435315	23.23799384032538
48	19.975784485277572	24.77707284470164	32.142989087904674	23.104153582116123
49	19.688456151781907	24.72602210631316	31.986142411408498	23.599379330496433
50	18.668616894435804	24.42173283670817	33.08843532845441	23.82121494040162
51	18.578774312074508	24.016013810568175	32.39186143754849	25.01335043980883
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	280.0
1	456.5
2	633.0
3	8367.5
4	16102.0
5	10866.5
6	5631.0
7	5522.5
8	5414.0
9	5509.5
10	5605.0
11	5594.0
12	5583.0
13	5377.0
14	5171.0
15	4998.5
16	4826.0
17	4588.0
18	4350.0
19	4092.5
20	3835.0
21	3753.0
22	3671.0
23	3863.5
24	4056.0
25	4429.0
26	5248.5
27	5695.0
28	6882.5
29	8070.0
30	9049.5
31	10029.0
32	11364.0
33	12699.0
34	14919.0
35	17139.0
36	18932.5
37	20726.0
38	22214.0
39	23702.0
40	26093.0
41	28484.0
42	30444.5
43	32405.0
44	35953.0
45	39501.0
46	47334.5
47	55168.0
48	56560.5
49	57953.0
50	54455.0
51	50957.0
52	44904.5
53	38852.0
54	33838.5
55	28825.0
56	26072.5
57	23320.0
58	21504.5
59	19689.0
60	18427.5
61	17166.0
62	15009.5
63	12853.0
64	10428.5
65	8004.0
66	6685.0
67	5366.0
68	4219.5
69	3073.0
70	2659.0
71	2245.0
72	1928.5
73	1612.0
74	1296.0
75	747.5
76	515.0
77	397.5
78	280.0
79	198.5
80	117.0
81	98.0
82	79.0
83	43.5
84	8.0
85	8.5
86	9.0
87	6.0
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	595486.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.17306188584265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.19676869442637	30.549081491851695
2	8.834587689898536	7.274940513841921
3	3.7448803519032245	4.62564571451963
4	2.3660344612922573	3.8966753319528995
5	1.7195714272587797	3.540001039582625
6	1.3804044292424407	3.4101286195614184
7	1.1064556605741023	3.1889317170730895
8	0.9606878872561907	3.164356946798285
9	0.7836248211561458	2.903780993106993
>10	4.8406498565159675	30.96389543170864
>50	0.0446326205972601	1.2568594123376673
>100	0.015559996139512035	0.9746350437312038
>500	0.004094735826187378	1.207967494954865
>1k	0.0016378943304749512	1.415168060383969
>5k	4.094735826187378E-4	1.6279321885951399
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9656	1.6215326640760654	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTT	3044	0.5111791041267133	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	2076	0.34862280557393455	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCT	1977	0.3319977295855822	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT	1297	0.2178052884534649	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	986	0.16557903964157006	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTC	904	0.15180877468152065	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCC	771	0.1294740766365624	No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	749	0.12577961530581744	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	746	0.1252758251243522	No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	695	0.1167113920394434	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGT	650	0.10915453931746506	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.679300604884078E-4	0.0	0.0	0.12326066439849132	0.0
2	1.679300604884078E-4	0.0	0.0	0.43728987751181386	0.0
3	1.679300604884078E-4	0.0	0.0	0.8038811995580081	0.0
4	1.679300604884078E-4	0.0	0.0	1.3360515612457724	0.0
5	1.679300604884078E-4	0.0	0.0	2.6114803706552294	0.0
6	1.679300604884078E-4	0.0	0.0	4.205640434871684	0.0
7	1.679300604884078E-4	0.0	0.0	5.385684969923727	0.0
8	1.679300604884078E-4	0.0	0.0	6.739705047641758	0.0
9	1.679300604884078E-4	0.0	0.0	7.498245130867896	0.0
10	1.679300604884078E-4	0.0	0.0	8.505321703616877	0.0
11	1.679300604884078E-4	0.0	0.0	9.565296245419708	0.0
12	1.679300604884078E-4	0.0	0.0	10.464561719335132	0.0
13	1.679300604884078E-4	0.0	0.0	10.926873175859718	0.0
14	1.679300604884078E-4	0.0	0.0	11.202614335181684	0.0
15	1.679300604884078E-4	0.0	0.0	11.464417299483111	0.0
16	1.679300604884078E-4	0.0	0.0	11.890120002821225	0.0
17	1.679300604884078E-4	0.0	0.0	12.418260043057268	0.0
18	1.679300604884078E-4	0.0	0.0	13.017266568819418	0.0
19	1.679300604884078E-4	0.0	0.0	13.412741861269618	0.0
20	1.679300604884078E-4	0.0	0.0	13.79226379797342	0.0
21	1.679300604884078E-4	0.0	0.0	14.234759507360375	0.0
22	1.679300604884078E-4	0.0	0.0	14.740732779611948	0.0
23	1.679300604884078E-4	0.0	0.0	15.26249147754943	0.0
24	1.679300604884078E-4	0.0	0.0	15.655447819092304	0.0
25	3.358601209768156E-4	0.0	0.0	16.029931853981452	0.0
26	3.358601209768156E-4	0.0	0.0	16.38594358221688	0.0
27	3.358601209768156E-4	0.0	0.0	16.759587966803586	0.0
28	3.358601209768156E-4	0.0	0.0	17.157246350040136	0.0
29	5.037901814652233E-4	0.0	0.0	17.566491907450384	0.0
30	5.037901814652233E-4	0.0	0.0	18.05231357244335	0.0
31	5.037901814652233E-4	0.0	0.0	18.49833581310056	0.0
32	5.037901814652233E-4	0.0	0.0	18.930587788797723	0.0
33	5.037901814652233E-4	0.0	0.0	19.341680576873344	0.0
34	5.037901814652233E-4	0.0	0.0	19.755964036098245	0.0
35	5.037901814652233E-4	0.0	0.0	20.177972278105614	0.0
36	5.037901814652233E-4	0.0	0.0	20.57915719261242	0.0
37	5.037901814652233E-4	0.0	0.0	20.989914120567065	0.0
38	5.037901814652233E-4	0.0	0.0	21.39328212586022	0.0
39	5.037901814652233E-4	0.0	0.0	21.8050466341778	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGG	20	7.030544E-4	45.000004	2
TGTTGCG	40	6.8048394E-9	45.000004	1
ACGTCGC	20	7.030544E-4	45.000004	20
CGTCGCT	20	7.030544E-4	45.000004	21
CGATAGA	20	7.030544E-4	45.000004	10
TAGTACG	20	7.030544E-4	45.000004	1
CGAACGG	55	1.8189894E-12	45.0	2
CGTTTTT	6230	0.0	44.13323	1
AGTACGG	75	0.0	42.0	2
CGTTTCT	300	0.0	41.25	1
CGACAGG	50	1.0804797E-9	40.499996	2
CGGTCTA	50	1.0804797E-9	40.499996	31
CGTTTTC	245	0.0	40.408165	1
TTACGAG	45	1.9255822E-8	40.0	1
GCTACGA	90	0.0	40.0	10
TGCGTAG	45	1.9255822E-8	40.0	1
GCGAACG	40	3.4544792E-7	39.375004	1
GTTAGCG	35	6.2432173E-6	38.571426	1
CGTTCTG	335	0.0	38.283585	1
GTTTTTT	7320	0.0	38.02254	2
>>END_MODULE