FastQCFastQC Report
Sat 18 Jun 2016
SRR3550544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625856
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78681.2571581961345741No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC44490.7108663973821454No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT25130.4015300644237652No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG22760.36366192862255853No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC20880.3336230698435423No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGC19100.30518202270170774No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG17820.28473003374578176No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC16330.2609226403517742No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT14970.23919240208610287No Hit
GAACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT14260.22784793946211268No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC12510.19988623581143264No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT11690.1867841803865426Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCC8800.140607424071991No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT7910.1263869005010737No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT7880.1259075570099192No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTC7280.1163206871868289No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC453.8380676E-1045.025
TTAGCCG253.8887625E-545.037
CACGACC700.045.027
TAGCACG406.8066583E-945.01
CGTTTTT45300.043.4602661
ACACGAC750.042.00000426
CACAACG1550.040.64516412
CGACGGT501.0804797E-940.528
ACGGGTA501.0804797E-940.55
CGTTCTG2850.040.2631571
GCGTGAG451.925946E-840.01
CGCATGG850.039.7058832
CGTGGCG1150.039.13043627
GTGGCGA1150.039.13043628
GCGAGAC750.039.00000421
TTACGCG356.2437284E-638.5714261
CGCACGG356.2437284E-638.5714262
TCGACGG356.2437284E-638.5714262
CAACGAG1650.038.18181614
CGTTTCT2200.037.8409081