Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550544_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 625856 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 7868 | 1.2571581961345741 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC | 4449 | 0.7108663973821454 | No Hit |
CGTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT | 2513 | 0.4015300644237652 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG | 2276 | 0.36366192862255853 | No Hit |
CGTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC | 2088 | 0.3336230698435423 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGC | 1910 | 0.30518202270170774 | No Hit |
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG | 1782 | 0.28473003374578176 | No Hit |
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC | 1633 | 0.2609226403517742 | No Hit |
CGTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT | 1497 | 0.23919240208610287 | No Hit |
GAACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT | 1426 | 0.22784793946211268 | No Hit |
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC | 1251 | 0.19988623581143264 | No Hit |
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT | 1169 | 0.1867841803865426 | Illumina Single End Adapter 1 (95% over 21bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCC | 880 | 0.140607424071991 | No Hit |
GAATGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT | 791 | 0.1263869005010737 | No Hit |
CGTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT | 788 | 0.1259075570099192 | No Hit |
CGTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTC | 728 | 0.1163206871868289 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCACGAC | 45 | 3.8380676E-10 | 45.0 | 25 |
TTAGCCG | 25 | 3.8887625E-5 | 45.0 | 37 |
CACGACC | 70 | 0.0 | 45.0 | 27 |
TAGCACG | 40 | 6.8066583E-9 | 45.0 | 1 |
CGTTTTT | 4530 | 0.0 | 43.460266 | 1 |
ACACGAC | 75 | 0.0 | 42.000004 | 26 |
CACAACG | 155 | 0.0 | 40.645164 | 12 |
CGACGGT | 50 | 1.0804797E-9 | 40.5 | 28 |
ACGGGTA | 50 | 1.0804797E-9 | 40.5 | 5 |
CGTTCTG | 285 | 0.0 | 40.263157 | 1 |
GCGTGAG | 45 | 1.925946E-8 | 40.0 | 1 |
CGCATGG | 85 | 0.0 | 39.705883 | 2 |
CGTGGCG | 115 | 0.0 | 39.130436 | 27 |
GTGGCGA | 115 | 0.0 | 39.130436 | 28 |
GCGAGAC | 75 | 0.0 | 39.000004 | 21 |
TTACGCG | 35 | 6.2437284E-6 | 38.571426 | 1 |
CGCACGG | 35 | 6.2437284E-6 | 38.571426 | 2 |
TCGACGG | 35 | 6.2437284E-6 | 38.571426 | 2 |
CAACGAG | 165 | 0.0 | 38.181816 | 14 |
CGTTTCT | 220 | 0.0 | 37.840908 | 1 |