##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550544_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	625856
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06553903773392	33.0	31.0	34.0	30.0	34.0
2	32.11519103435934	33.0	31.0	34.0	30.0	34.0
3	32.039149580734225	33.0	31.0	34.0	30.0	34.0
4	35.75624904131302	37.0	35.0	37.0	33.0	37.0
5	35.85137635494427	37.0	35.0	37.0	35.0	37.0
6	35.80274855557828	37.0	35.0	37.0	35.0	37.0
7	36.10207300081808	37.0	35.0	37.0	35.0	37.0
8	35.93679856069128	37.0	35.0	37.0	35.0	37.0
9	37.79463646589631	39.0	38.0	39.0	35.0	39.0
10	37.44825487012987	39.0	37.0	39.0	34.0	39.0
11	37.409517524798034	39.0	37.0	39.0	34.0	39.0
12	37.4231340755701	39.0	37.0	39.0	35.0	39.0
13	37.437218785151856	39.0	37.0	39.0	35.0	39.0
14	38.63486808467123	40.0	38.0	41.0	35.0	41.0
15	38.717721009305656	40.0	38.0	41.0	35.0	41.0
16	38.4752259305655	40.0	38.0	41.0	34.0	41.0
17	38.525633692095305	40.0	38.0	41.0	34.0	41.0
18	38.355351710297576	40.0	38.0	41.0	34.0	41.0
19	38.13544809029553	40.0	37.0	41.0	34.0	41.0
20	38.20549775028122	40.0	37.0	41.0	34.0	41.0
21	38.12560716842213	40.0	36.0	41.0	34.0	41.0
22	38.243153377134675	40.0	37.0	41.0	34.0	41.0
23	38.250885187647	40.0	37.0	41.0	34.0	41.0
24	38.17596859341446	40.0	36.0	41.0	34.0	41.0
25	37.94454475150833	40.0	36.0	41.0	34.0	41.0
26	38.03318175426935	40.0	36.0	41.0	34.0	41.0
27	38.047202551385624	40.0	36.0	41.0	34.0	41.0
28	37.94594603231414	40.0	36.0	41.0	34.0	41.0
29	37.90934336332958	40.0	36.0	41.0	34.0	41.0
30	37.672122660803765	40.0	36.0	41.0	33.0	41.0
31	37.59221929645158	40.0	36.0	41.0	33.0	41.0
32	37.4164216688823	40.0	36.0	41.0	33.0	41.0
33	37.13639559259638	40.0	35.0	41.0	32.0	41.0
34	36.911462061560485	40.0	35.0	41.0	31.0	41.0
35	36.735632477758465	40.0	35.0	41.0	31.0	41.0
36	36.58354957050823	40.0	35.0	41.0	30.0	41.0
37	36.542412312097355	40.0	35.0	41.0	30.0	41.0
38	36.4045355481133	40.0	35.0	41.0	30.0	41.0
39	36.31669105992433	39.0	35.0	41.0	30.0	41.0
40	36.23803239083751	39.0	35.0	41.0	29.0	41.0
41	36.083736514469784	39.0	35.0	41.0	28.0	41.0
42	36.068947169956026	39.0	35.0	41.0	28.0	41.0
43	35.9937988930361	39.0	35.0	41.0	28.0	41.0
44	35.88183543818386	39.0	35.0	41.0	27.0	41.0
45	35.86429785765416	39.0	35.0	41.0	27.0	41.0
46	35.72786391757848	39.0	35.0	41.0	27.0	41.0
47	35.62091918907864	39.0	35.0	41.0	26.0	41.0
48	35.510665392678185	39.0	35.0	41.0	26.0	41.0
49	35.44199304632375	39.0	35.0	41.0	26.0	41.0
50	35.322326541568664	38.0	35.0	40.0	26.0	41.0
51	34.1794038884344	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	12.0
10	12.0
11	9.0
12	6.0
13	10.0
14	12.0
15	23.0
16	45.0
17	85.0
18	167.0
19	311.0
20	570.0
21	946.0
22	1528.0
23	2294.0
24	3752.0
25	5962.0
26	8636.0
27	9639.0
28	9321.0
29	8936.0
30	9304.0
31	11134.0
32	13844.0
33	18075.0
34	30986.0
35	41692.0
36	43983.0
37	64484.0
38	121527.0
39	218421.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.52483638408835	20.290130637079457	23.898468657326923	17.286564321505267
2	35.92359903875652	24.913079046937312	24.228097198077513	14.935224716228653
3	28.53819408937519	24.681236578382247	31.91756570201452	14.86300363022804
4	25.46751968503937	24.58680591062481	33.10154412516617	16.84413027916965
5	24.97555348195112	28.001489160445853	29.18930233152674	17.833655026076286
6	23.831041006237857	32.802433786685754	28.75389866039472	14.612626546681664
7	76.04049493813272	4.679990285305246	14.707856120257695	4.571658656304326
8	77.31027584620104	5.00386670416198	13.278453829634932	4.407403620002045
9	70.96201043051437	6.98403466612128	14.807240004090398	7.246714899273954
10	33.768151140198384	32.85659960118622	20.606177778914	12.769071479701402
11	23.840947438388383	22.974933531035894	34.29862460374272	18.88549442683301
12	22.446057879128745	21.68885494426833	35.6930348195112	20.172052357091726
13	21.312570303712036	23.251834287759486	37.29979420186113	18.13580120666735
14	18.66883116883117	27.45184195725534	34.511453113815314	19.36787376009817
15	17.846277738010023	25.7913961038961	36.98151012373454	19.38081603435934
16	20.575180233152672	26.946294355251048	33.31117701196442	19.167348399631866
17	20.771711064526023	25.735312915431024	32.41879921259843	21.074176807444527
18	21.519646691890788	25.380598731976683	32.56308160343593	20.536672972696596
19	20.401178545863587	27.53924225380918	31.005055476020043	21.054523724307188
20	22.32238725841088	27.2633001840679	30.978691584006548	19.435620973514673
21	21.654981337560077	27.27304683505471	32.52026025155946	18.55171157582575
22	21.239070968401677	23.997053635341036	32.18072527865835	22.583150117598937
23	20.19633909397689	26.513926526229675	32.32021423458431	20.969520145209124
24	20.678079302587175	25.407601748645053	32.64920365068003	21.26511529808774
25	20.634778607219552	28.277431230187133	30.088390939768892	20.99939922282442
26	20.33087483382759	26.838282288577563	31.008410880458126	21.82243199713672
27	21.50015338991717	25.75896052766132	30.962234124143574	21.77865195827794
28	19.463902239492793	26.190529450864098	31.842788117394417	22.502780192248697
29	22.22108600061356	24.274273954392065	31.343951324266285	22.160688720728093
30	21.936515748031496	23.991940638102054	33.56906381020554	20.502479803660904
31	22.869637744145617	24.506755547602005	30.19848016157071	22.425126546681664
32	24.335470140096124	25.05320712751815	29.853352847939462	20.75796988444626
33	22.632842059515287	24.375575212189386	31.060179977502813	21.931402750792515
34	21.855187135698948	24.587125472952245	32.38204954494325	21.17563784640556
35	22.880502863278455	25.00623146538501	31.25511299723898	20.858152674097553
36	22.629486655077205	27.64709453931895	29.653945955619182	20.06947284998466
37	22.103487064116987	26.521915584415584	31.049634420697416	20.32496293077002
38	21.66872251763984	26.846910471418344	29.904802382656715	21.5795646282851
39	22.687007874015748	25.93184374680438	30.035822936905614	21.34532544227426
40	23.476326822783516	24.452270170774106	32.07495014827692	19.996452858165455
41	21.646353154719296	26.48133116883117	29.953535637590754	21.918780038858777
42	22.586825084364452	26.53246114122098	29.58795633500358	21.292757439410984
43	22.550235197872993	25.67491563554556	29.36346379997955	22.411385366601902
44	22.30129614480008	24.984661008283055	29.111329123632274	23.60271372328459
45	22.30816673483996	23.999130790469373	29.313132733408327	24.37956974128234
46	23.47936266489416	25.058320124757135	29.177478525411598	22.28483868493711
47	21.060116065037324	25.050970191226096	31.735415175375802	22.153498568360774
48	21.78775948460988	24.875210911136108	30.631806677574392	22.705222926679618
49	22.187691737396463	23.810269454954494	31.87905844155844	22.1229803660906
50	20.821562787606098	23.512277584620104	32.24559643112793	23.420563196645876
51	20.98533848041722	22.82665661110543	30.704826669393597	25.483178239083752
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	261.0
1	460.0
2	659.0
3	6441.0
4	12223.0
5	8015.5
6	3808.0
7	3734.0
8	3660.0
9	3736.5
10	3813.0
11	3817.0
12	3821.0
13	3742.5
14	3664.0
15	3650.5
16	3637.0
17	3422.0
18	3207.0
19	3203.5
20	3200.0
21	3220.0
22	3240.0
23	3240.5
24	3241.0
25	3745.5
26	4870.0
27	5490.0
28	6420.0
29	7350.0
30	8789.0
31	10228.0
32	11422.0
33	12616.0
34	15066.0
35	17516.0
36	18770.5
37	20025.0
38	21670.0
39	23315.0
40	25741.0
41	28167.0
42	30977.5
43	33788.0
44	38103.5
45	42419.0
46	55423.0
47	68427.0
48	66292.5
49	64158.0
50	61589.0
51	59020.0
52	50927.0
53	42834.0
54	37528.5
55	32223.0
56	28828.5
57	25434.0
58	23618.5
59	21803.0
60	19778.5
61	17754.0
62	15684.5
63	13615.0
64	11328.0
65	9041.0
66	7732.5
67	6424.0
68	5255.0
69	4086.0
70	3512.5
71	2939.0
72	2398.5
73	1858.0
74	1557.5
75	1053.0
76	849.0
77	584.5
78	320.0
79	221.0
80	122.0
81	89.5
82	57.0
83	36.0
84	15.0
85	12.0
86	9.0
87	8.0
88	7.0
89	5.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	625856.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.88912379186868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.30850107973562	27.77594872406442
2	8.983136737115572	6.807263597437103
3	3.7653365675385304	4.279959099765518
4	2.3014614377905924	3.4880142927463935
5	1.6975607059521298	3.215954386601612
6	1.3181582541568397	2.99663167614132
7	1.1337719189907995	3.0070337213270832
8	0.9741657057865265	2.952822801627106
9	0.87873148936732	2.9964929562406377
>10	5.549067524147365	34.004168097978706
>50	0.06303370112052736	1.6256412391401343
>100	0.019037023803404253	1.2734884275098
>500	0.0029613148138628843	0.7900597179604537
>1k	0.004653494707498818	3.525375012542548
>5k	4.2304497340898343E-4	1.2611462489171938
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7868	1.2571581961345741	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC	4449	0.7108663973821454	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT	2513	0.4015300644237652	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG	2276	0.36366192862255853	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC	2088	0.3336230698435423	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGC	1910	0.30518202270170774	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG	1782	0.28473003374578176	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	1633	0.2609226403517742	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT	1497	0.23919240208610287	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT	1426	0.22784793946211268	No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	1251	0.19988623581143264	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	1169	0.1867841803865426	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCC	880	0.140607424071991	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT	791	0.1263869005010737	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT	788	0.1259075570099192	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTC	728	0.1163206871868289	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.7934349115451476E-4	0.0	0.0	0.2019633909397689	0.0
2	4.7934349115451476E-4	0.0	0.0	0.7993851620820125	0.0
3	4.7934349115451476E-4	0.0	0.0	1.3098859801615708	0.0
4	4.7934349115451476E-4	0.0	0.0	2.0102068207383166	0.0
5	4.7934349115451476E-4	0.0	0.0	4.035912414357297	0.0
6	4.7934349115451476E-4	0.0	0.0	5.5397727272727275	0.0
7	4.7934349115451476E-4	0.0	0.0	6.722313119950916	0.0
8	4.7934349115451476E-4	0.0	0.0	8.126949330197363	0.0
9	4.7934349115451476E-4	0.0	0.0	8.848361284384906	0.0
10	4.7934349115451476E-4	0.0	0.0	10.216407608139892	0.0
11	4.7934349115451476E-4	0.0	0.0	11.462061560486758	0.0
12	4.7934349115451476E-4	0.0	0.0	12.737115246957767	0.0
13	6.391246548726864E-4	0.0	0.0	13.244580222926679	0.0
14	6.391246548726864E-4	0.0	0.0	13.53026894365477	0.0
15	6.391246548726864E-4	0.0	0.0	13.873159320993967	0.0
16	7.98905818590858E-4	0.0	0.0	14.379026485325697	0.0
17	7.98905818590858E-4	0.0	0.0	15.013837048777994	0.0
18	7.98905818590858E-4	0.0	0.0	15.73764572042131	0.0
19	7.98905818590858E-4	0.0	0.0	16.259011657633703	0.0
20	7.98905818590858E-4	0.0	0.0	16.716784691686268	0.0
21	7.98905818590858E-4	0.0	0.0	17.22760507209326	0.0
22	7.98905818590858E-4	0.0	0.0	17.83605174353206	0.0
23	7.98905818590858E-4	0.0	0.0	18.37946748133756	0.0
24	9.586869823090295E-4	0.0	0.0	18.849383883832704	0.0
25	9.586869823090295E-4	0.0	0.0	19.226946773698742	0.0
26	9.586869823090295E-4	0.0	0.0	19.600035790980673	0.0
27	9.586869823090295E-4	0.0	0.0	20.00044738725841	0.0
28	9.586869823090295E-4	0.0	0.0	20.398781828407813	0.0
29	0.0011184681460272012	0.0	0.0	20.831309438592903	0.0
30	0.0011184681460272012	0.0	0.0	21.382394672256876	0.0
31	0.0011184681460272012	0.0	0.0	21.831859085796093	0.0
32	0.0011184681460272012	0.0	0.0	22.289632119848655	0.0
33	0.0011184681460272012	0.0	0.0	22.71193373555578	0.0
34	0.0011184681460272012	0.0	0.0	23.145739595050618	0.0
35	0.0011184681460272012	0.0	0.0	23.581942172001227	0.0
36	0.0011184681460272012	0.0	0.0	23.982673330606403	0.0
37	0.0012782493097453728	0.0	0.0	24.40225866653032	0.0
38	0.0014380304734635443	0.0	0.0	24.826477656202066	0.0
39	0.0014380304734635443	0.0	0.0	25.287925657020146	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	45	3.8380676E-10	45.0	25
TTAGCCG	25	3.8887625E-5	45.0	37
CACGACC	70	0.0	45.0	27
TAGCACG	40	6.8066583E-9	45.0	1
CGTTTTT	4530	0.0	43.460266	1
ACACGAC	75	0.0	42.000004	26
CACAACG	155	0.0	40.645164	12
CGACGGT	50	1.0804797E-9	40.5	28
ACGGGTA	50	1.0804797E-9	40.5	5
CGTTCTG	285	0.0	40.263157	1
GCGTGAG	45	1.925946E-8	40.0	1
CGCATGG	85	0.0	39.705883	2
CGTGGCG	115	0.0	39.130436	27
GTGGCGA	115	0.0	39.130436	28
GCGAGAC	75	0.0	39.000004	21
TTACGCG	35	6.2437284E-6	38.571426	1
CGCACGG	35	6.2437284E-6	38.571426	2
TCGACGG	35	6.2437284E-6	38.571426	2
CAACGAG	165	0.0	38.181816	14
CGTTTCT	220	0.0	37.840908	1
>>END_MODULE