FastQCFastQC Report
Sat 18 Jun 2016
SRR3550543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927038
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56230.6065555025791823No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC16110.1737792841285902No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT9610.10366349599477045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG302.1650449E-645.0000041
CGTAAGT207.032581E-445.044
TAAGACG253.8902195E-545.01
TTACGGG1900.041.4473723
CGTTTTT31050.040.0724641
CGACAGG900.040.02
CGACGGT800.039.37528
CGGTCTA800.039.37531
AGACACG2300.039.13043224
ACGGGAC3400.039.044125
GCGAACG356.246979E-638.571431
TCGATAG356.246979E-638.571431
ATTAGCG659.094947E-1238.076921
ATTCGGC659.094947E-1238.0769217
TGATTCG659.094947E-1238.0769215
CGTAAGG1250.037.82
TATACCG301.1397513E-437.5000041
ACAACGA2100.037.513
TAGGGCG2400.036.5625045
CGAGACA2550.036.1764722
Produced by FastQC (version 0.11.5)