##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550543_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	927038
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23175533257536	33.0	31.0	34.0	30.0	34.0
2	32.25384827806411	34.0	31.0	34.0	30.0	34.0
3	32.237645058778604	34.0	31.0	34.0	30.0	34.0
4	35.8855149411351	37.0	35.0	37.0	35.0	37.0
5	35.958364166301706	37.0	35.0	37.0	35.0	37.0
6	35.9105505923166	37.0	35.0	37.0	35.0	37.0
7	36.1809397241537	37.0	35.0	37.0	35.0	37.0
8	36.02675834216073	37.0	36.0	37.0	35.0	37.0
9	37.89390941903137	39.0	38.0	39.0	35.0	39.0
10	37.506677180439205	39.0	37.0	39.0	35.0	39.0
11	37.47176922628846	39.0	37.0	39.0	35.0	39.0
12	37.41079869433615	39.0	37.0	39.0	35.0	39.0
13	37.41609836921464	39.0	37.0	39.0	35.0	39.0
14	38.61385293806726	40.0	38.0	41.0	35.0	41.0
15	38.723558257590305	40.0	38.0	41.0	35.0	41.0
16	38.48053693591849	40.0	38.0	41.0	34.0	41.0
17	38.532493813630076	40.0	38.0	41.0	34.0	41.0
18	38.41849201435108	40.0	38.0	41.0	34.0	41.0
19	38.241003065678	40.0	37.0	41.0	34.0	41.0
20	38.3989642280036	40.0	37.0	41.0	34.0	41.0
21	38.28839702363873	40.0	37.0	41.0	34.0	41.0
22	38.394057201538665	40.0	37.0	41.0	34.0	41.0
23	38.41515666024478	40.0	37.0	41.0	34.0	41.0
24	38.355737305266885	40.0	37.0	41.0	34.0	41.0
25	38.101554628828595	40.0	37.0	41.0	34.0	41.0
26	38.198210860827714	40.0	37.0	41.0	34.0	41.0
27	38.19038701757641	40.0	37.0	41.0	34.0	41.0
28	38.08772024447757	40.0	37.0	41.0	34.0	41.0
29	38.07352341543712	40.0	37.0	41.0	34.0	41.0
30	37.88642536767641	40.0	36.0	41.0	34.0	41.0
31	37.834526740004186	40.0	36.0	41.0	34.0	41.0
32	37.74981176607647	40.0	36.0	41.0	33.0	41.0
33	37.633605094936776	40.0	36.0	41.0	33.0	41.0
34	37.51017218280157	40.0	36.0	41.0	33.0	41.0
35	37.39935795512158	40.0	36.0	41.0	33.0	41.0
36	37.270692247782726	40.0	36.0	41.0	33.0	41.0
37	37.247299463452414	40.0	35.0	41.0	33.0	41.0
38	37.124459838755264	40.0	35.0	41.0	32.0	41.0
39	37.06479130305338	40.0	35.0	41.0	32.0	41.0
40	36.97388780179453	40.0	35.0	41.0	32.0	41.0
41	36.85377082708584	39.0	35.0	41.0	31.0	41.0
42	36.83220644676917	39.0	35.0	41.0	31.0	41.0
43	36.75564216353591	39.0	35.0	41.0	31.0	41.0
44	36.63035711588953	39.0	35.0	41.0	31.0	41.0
45	36.572054219999615	39.0	35.0	41.0	31.0	41.0
46	36.45784530946951	39.0	35.0	41.0	31.0	41.0
47	36.39984768693409	39.0	35.0	41.0	31.0	41.0
48	36.28406063181876	39.0	35.0	41.0	31.0	41.0
49	36.223077155413264	39.0	35.0	41.0	30.0	41.0
50	36.11179153389613	38.0	35.0	41.0	30.0	41.0
51	35.00483798938124	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	15.0
10	26.0
11	22.0
12	18.0
13	23.0
14	35.0
15	48.0
16	64.0
17	132.0
18	244.0
19	403.0
20	603.0
21	1023.0
22	1513.0
23	2353.0
24	3518.0
25	5390.0
26	7768.0
27	9385.0
28	10050.0
29	10713.0
30	12292.0
31	14739.0
32	18699.0
33	25738.0
34	50179.0
35	67331.0
36	63179.0
37	96399.0
38	178978.0
39	345937.0
40	208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.330883955134524	21.33580284734822	23.92836108120703	13.404952116310225
2	33.31341325814044	23.95468146936803	25.325391192162567	17.40651408032896
3	32.68819616887333	22.910279837503964	28.172307931282216	16.229216062340488
4	28.811116696402948	25.22884714542446	27.41441019677726	18.545625961395327
5	25.582446458505476	30.54027990222623	25.505211221114994	18.372062418153302
6	26.749928266155216	35.015500982699734	23.954465728481463	14.28010502266358
7	80.92321997588017	6.027261018426429	8.954217626461915	4.095301379231488
8	83.13823165824917	4.952116310226765	8.413031612512109	3.4966204190119496
9	76.7776509700789	7.544458803199006	10.331723187183266	5.346167039538832
10	39.771724567924934	29.10463217257545	17.1127828632699	14.010860396229713
11	30.304906594983162	25.590860353081535	25.07275861399425	19.031474437941057
12	27.673730742429115	22.990211835976517	29.222858178413397	20.113199243180972
13	25.070816946015157	23.166472140300616	30.944578323650163	20.818132590034068
14	21.6162659998835	26.370979398902744	30.244067664971663	21.768686936242094
15	21.472690439874093	25.842090615487173	32.24506438786759	20.440154556771137
16	25.291196261641918	24.50881193651177	30.630675333697216	19.569316468149093
17	23.933646732927887	25.10511974697909	28.540577624649693	22.420655895443335
18	25.361312049775737	25.10835586027757	28.502283617284295	21.028048472662395
19	25.678235412140605	26.493951704245134	27.201797553066864	20.626015330547396
20	26.896847809906387	26.10475514488079	27.47104217950073	19.527354865712084
21	25.749537775150532	26.151570917265527	28.24760150069361	19.851289806890332
22	24.817968626960276	23.896323559552034	28.29312282775895	22.99258498572874
23	23.961800918624697	25.886856849449536	28.479091471978496	21.67225075994727
24	23.70355907740567	25.063266014985363	29.2830498857652	21.950125021843764
25	24.467281815847894	25.74651740273862	27.55399455038521	22.232206231028286
26	23.32784632345168	26.909792263100325	27.295860579609467	22.466500833838527
27	23.58792196220651	25.694092367303174	28.623853606864014	22.0941320636263
28	22.0201329395343	26.841402402059032	29.203441498622496	21.935023159784173
29	24.041840787540533	26.490176238730236	27.483447280478256	21.98453569325098
30	23.89233235315057	25.349662041901194	28.7575050860914	22.000500518856832
31	24.596726347787254	25.989225900124914	26.784878289778845	22.629169462308987
32	23.951337485626265	26.719185189819616	27.406859265747467	21.922618058806652
33	23.337338922460567	27.376224059855154	26.475182247113928	22.811254770570354
34	22.287975250205495	26.798685706519045	28.330338130691512	22.58300091258395
35	22.700687566205485	26.28252563541085	28.040598120033916	22.976188678349754
36	22.146233487731894	28.63798463493406	26.4464887092007	22.769293168133345
37	22.365210487595977	28.29517236618132	26.811522289269696	22.528094856953004
38	22.102330217315792	28.437561351314617	25.89311333515994	23.56699509620965
39	23.52190525091744	25.870244801183983	26.586720285468342	24.021129662430234
40	22.769724649906475	25.496150103879238	28.89007786088596	22.844047385328324
41	20.92772896040939	26.319848808786695	28.445435893674258	24.306986337129654
42	22.18495897687042	26.902457072957098	26.851110741954482	24.061473208218
43	22.628630110092573	25.511359836382113	27.822268342829524	24.037741710695787
44	22.971226637958747	26.031403243448487	27.169220679195462	23.828149439397308
45	23.371857464311063	25.25840364688395	27.109352583173507	24.260386305631485
46	22.793456147428692	26.233660324603736	27.06490996054099	23.907973567426577
47	22.154431641421386	25.866792946998935	28.595375809837353	23.383399601742326
48	21.65984565896975	25.443617198000513	28.41415346512225	24.482383677907485
49	22.240296514274497	25.648355299351266	28.370358065149432	23.740990121224804
50	20.869263180150114	25.493453342797167	29.14238682772443	24.49489664932829
51	21.022654950498254	25.23434853803188	28.230989452428055	25.512007059041807
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	334.0
1	855.5
2	1377.0
3	5700.5
4	10024.0
5	6671.5
6	3319.0
7	3138.0
8	2957.0
9	2917.0
10	2877.0
11	2944.0
12	3011.0
13	2999.0
14	2987.0
15	2918.5
16	2850.0
17	2755.5
18	2661.0
19	2659.0
20	2657.0
21	2605.0
22	2553.0
23	2896.5
24	3240.0
25	4250.0
26	6538.5
27	7817.0
28	9305.0
29	10793.0
30	12926.5
31	15060.0
32	16213.5
33	17367.0
34	20064.5
35	22762.0
36	24561.5
37	26361.0
38	28478.5
39	30596.0
40	35037.0
41	39478.0
42	42886.0
43	46294.0
44	50686.5
45	55079.0
46	62749.5
47	70420.0
48	76013.5
49	81607.0
50	80071.5
51	78536.0
52	72805.0
53	67074.0
54	61600.5
55	56127.0
56	54002.0
57	51877.0
58	50137.0
59	48397.0
60	46796.0
61	45195.0
62	40702.0
63	36209.0
64	30948.0
65	25687.0
66	21820.5
67	17954.0
68	14518.0
69	11082.0
70	9323.0
71	7564.0
72	6323.0
73	5082.0
74	4084.5
75	2452.5
76	1818.0
77	1337.0
78	856.0
79	622.0
80	388.0
81	298.0
82	208.0
83	128.5
84	49.0
85	58.5
86	68.0
87	45.0
88	22.0
89	14.0
90	6.0
91	7.0
92	8.0
93	4.0
94	0.0
95	0.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	927038.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.233838572435598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.43876660396653	20.169906041102948
2	8.489775692952021	4.793979128619898
3	3.1802926606620665	2.693756087827035
4	1.8290119985096407	2.065601180518761
5	1.28227083459531	1.8101713875053125
6	1.0897991655497563	1.8461528229904107
7	0.9309780413262102	1.8399558613300548
8	0.8123472319890817	1.8348544490195702
9	0.7759586414239715	1.9717461918765766
>10	10.027028906331765	55.333888829850196
>50	0.10148486634873626	1.9117243282347194
>100	0.03613475905838194	1.8654885250225235
>500	0.005381772625716311	1.077637628865973
>1k	3.8441233040830796E-4	0.17484884883705132
>5k	3.8441233040830796E-4	0.6102886883989693
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5623	0.6065555025791823	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1611	0.1737792841285902	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT	961	0.10366349599477045	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.236113298483989E-4	0.0	0.0	0.09665191718138846	0.0
2	4.314817731311985E-4	0.0	0.0	0.3190807712305213	0.0
3	4.314817731311985E-4	0.0	0.0	0.5306147105080914	0.0
4	4.314817731311985E-4	0.0	0.0	0.7808741389241864	0.0
5	4.314817731311985E-4	0.0	0.0	1.4641255266774393	0.0
6	4.314817731311985E-4	0.0	0.0	2.1466218213277126	0.0
7	4.314817731311985E-4	0.0	0.0	2.7046356244296352	0.0
8	4.314817731311985E-4	0.0	0.0	3.4765565165613492	0.0
9	4.314817731311985E-4	0.0	0.0	3.8785896586763435	0.0
10	4.314817731311985E-4	0.0	0.0	4.549759556781923	0.0
11	4.314817731311985E-4	0.0	0.0	5.3583563996297885	0.0
12	4.314817731311985E-4	0.0	0.0	6.002450816471385	0.0
13	4.314817731311985E-4	0.0	0.0	6.294563976881206	0.0
14	4.314817731311985E-4	0.0	0.0	6.445151115703995	0.0
15	4.314817731311985E-4	0.0	0.0	6.663588763351664	0.0
16	4.314817731311985E-4	0.0	0.0	7.036173274450454	0.0
17	4.314817731311985E-4	0.0	0.0	7.48221755742483	0.0
18	4.314817731311985E-4	0.0	0.0	7.957818341858694	0.0
19	4.314817731311985E-4	0.0	0.0	8.2732315180176	0.0
20	5.393522164139982E-4	0.0	0.0	8.587134507970546	0.0
21	5.393522164139982E-4	0.0	0.0	8.968240784088678	0.0
22	5.393522164139982E-4	0.0	0.0	9.371676241966348	0.0
23	6.472226596967978E-4	0.0	0.0	9.777592720039523	0.0
24	6.472226596967978E-4	0.0	0.0	10.125582770069835	0.0
25	6.472226596967978E-4	0.0	0.0	10.433013533425815	0.0
26	6.472226596967978E-4	0.0	0.0	10.717683633249122	0.0
27	6.472226596967978E-4	0.0	0.0	11.0150824453798	0.0
28	7.550931029795974E-4	0.0	0.0	11.329740528435728	0.0
29	7.550931029795974E-4	0.0	0.0	11.650762967645338	0.0
30	7.550931029795974E-4	0.0	0.0	12.027015073815745	0.0
31	7.550931029795974E-4	0.0	0.0	12.355372703168586	0.0
32	7.550931029795974E-4	0.0	0.0	12.712100259104805	0.0
33	7.550931029795974E-4	0.0	0.0	13.05448104608441	0.0
34	7.550931029795974E-4	0.0	0.0	13.375287744407457	0.0
35	7.550931029795974E-4	0.0	0.0	13.727269000839232	0.0
36	7.550931029795974E-4	0.0	0.0	14.05584237107864	0.0
37	7.550931029795974E-4	0.0	0.0	14.400596307810467	0.0
38	7.550931029795974E-4	0.0	0.0	14.757323863746686	0.0
39	7.550931029795974E-4	0.0	0.0	15.130663467948455	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	30	2.1650449E-6	45.000004	1
CGTAAGT	20	7.032581E-4	45.0	44
TAAGACG	25	3.8902195E-5	45.0	1
TTACGGG	190	0.0	41.447372	3
CGTTTTT	3105	0.0	40.072464	1
CGACAGG	90	0.0	40.0	2
CGACGGT	80	0.0	39.375	28
CGGTCTA	80	0.0	39.375	31
AGACACG	230	0.0	39.130432	24
ACGGGAC	340	0.0	39.04412	5
GCGAACG	35	6.246979E-6	38.57143	1
TCGATAG	35	6.246979E-6	38.57143	1
ATTAGCG	65	9.094947E-12	38.07692	1
ATTCGGC	65	9.094947E-12	38.07692	17
TGATTCG	65	9.094947E-12	38.07692	15
CGTAAGG	125	0.0	37.8	2
TATACCG	30	1.1397513E-4	37.500004	1
ACAACGA	210	0.0	37.5	13
TAGGGCG	240	0.0	36.562504	5
CGAGACA	255	0.0	36.17647	22
>>END_MODULE