FastQCFastQC Report
Sat 18 Jun 2016
SRR3550542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949860
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121631.2805044953993219No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT21570.22708609689849032No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT13560.1427578801086476No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC12400.13054555408165414No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA11500.12107047354346957No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10190.10727896742677867No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9850.10369949255679785No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAT207.032668E-445.010
ACGGTAC207.032668E-445.013
TCACGAC950.042.6315825
CACGACG950.042.6315826
CGTTTTT68250.042.4615361
GGCGATA3250.042.230778
GCGATAT603.6379788E-1241.2500049
CTATGCG451.927765E-840.0000041
CGAATAT750.039.014
CGGTCTA1050.038.5714331
CGACGGT1100.036.8181828
TACGAGG1100.036.818182
GCGATAA1100.036.818189
GTTTTTT83850.036.118072
TACGGTA250.002106924836.029
GTATGCG250.002106924836.01
TTCGGTA250.002106924836.025
CGATACG250.002106924836.012
CGAGGGA5000.035.554
TACGGGA2600.035.4807664
Produced by FastQC (version 0.11.5)