##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550542_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	949860
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21876065946561	33.0	31.0	34.0	30.0	34.0
2	32.25642936853852	34.0	31.0	34.0	30.0	34.0
3	32.18149306213547	33.0	31.0	34.0	30.0	34.0
4	35.87674078285221	37.0	35.0	37.0	35.0	37.0
5	35.95578295748847	37.0	35.0	37.0	35.0	37.0
6	35.907599014591625	37.0	35.0	37.0	35.0	37.0
7	36.16339249994736	37.0	35.0	37.0	35.0	37.0
8	35.9960099383067	37.0	35.0	37.0	35.0	37.0
9	37.86672667551007	39.0	38.0	39.0	35.0	39.0
10	37.52924852083465	39.0	37.0	39.0	35.0	39.0
11	37.47473311856484	39.0	37.0	39.0	35.0	39.0
12	37.42869054386962	39.0	37.0	39.0	35.0	39.0
13	37.42525424799444	39.0	37.0	39.0	35.0	39.0
14	38.64016276082791	40.0	38.0	41.0	35.0	41.0
15	38.75176868170046	40.0	38.0	41.0	35.0	41.0
16	38.51934074495189	40.0	38.0	41.0	34.0	41.0
17	38.56767523635062	40.0	38.0	41.0	34.0	41.0
18	38.38804560672099	40.0	38.0	41.0	34.0	41.0
19	38.16735308361232	40.0	37.0	41.0	34.0	41.0
20	38.23969321794791	40.0	36.0	41.0	34.0	41.0
21	38.13022340134336	40.0	36.0	41.0	34.0	41.0
22	38.215239087865584	40.0	36.0	41.0	34.0	41.0
23	38.23330701366517	40.0	36.0	41.0	34.0	41.0
24	38.155086012675554	40.0	36.0	41.0	34.0	41.0
25	37.90218137409723	40.0	35.0	41.0	34.0	41.0
26	37.969576569178614	40.0	36.0	41.0	34.0	41.0
27	37.98665592824206	40.0	36.0	41.0	34.0	41.0
28	37.875944876087004	40.0	35.0	41.0	34.0	41.0
29	37.83073084454551	40.0	35.0	41.0	34.0	41.0
30	37.62810519444971	40.0	35.0	41.0	33.0	41.0
31	37.532781673088664	40.0	35.0	41.0	33.0	41.0
32	37.36568336386415	40.0	35.0	41.0	33.0	41.0
33	37.154136399048284	40.0	35.0	41.0	33.0	41.0
34	36.93614427389299	40.0	35.0	41.0	32.0	41.0
35	36.781812056513594	40.0	35.0	41.0	31.0	41.0
36	36.62682816415051	40.0	35.0	41.0	31.0	41.0
37	36.57206640978671	40.0	35.0	41.0	31.0	41.0
38	36.42278546312088	40.0	35.0	41.0	30.0	41.0
39	36.33916577179795	39.0	35.0	41.0	30.0	41.0
40	36.23567262543954	39.0	35.0	41.0	30.0	41.0
41	36.078088349862085	39.0	35.0	41.0	29.0	41.0
42	36.043895942559956	39.0	35.0	41.0	29.0	41.0
43	35.96698250268461	39.0	35.0	41.0	29.0	41.0
44	35.83191312403933	39.0	35.0	41.0	28.0	41.0
45	35.81239024698377	39.0	35.0	41.0	28.0	41.0
46	35.691363990482806	39.0	35.0	41.0	27.0	41.0
47	35.645600404270105	39.0	35.0	41.0	27.0	41.0
48	35.516893015812855	38.0	35.0	41.0	27.0	41.0
49	35.46158065399111	38.0	35.0	40.0	27.0	41.0
50	35.359667740509124	38.0	35.0	40.0	27.0	41.0
51	34.2844977154528	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	14.0
9	20.0
10	21.0
11	18.0
12	32.0
13	20.0
14	29.0
15	50.0
16	88.0
17	143.0
18	298.0
19	511.0
20	838.0
21	1287.0
22	1947.0
23	3037.0
24	4799.0
25	8107.0
26	11931.0
27	14527.0
28	14575.0
29	13933.0
30	14372.0
31	16390.0
32	20123.0
33	27174.0
34	52143.0
35	68744.0
36	65787.0
37	98080.0
38	177174.0
39	333449.0
40	197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.69553407870634	20.336575916450844	22.69692375718527	17.270966247657547
2	37.33823931947866	22.794411808055923	23.526414418967008	16.34093445349841
3	31.162065988672015	22.58932895374055	30.550081064577938	15.698523993009497
4	28.390920767270966	24.5328785294675	29.55993514833765	17.516265554923883
5	24.520034531404626	29.187564483186996	28.029814920093486	18.26258606531489
6	25.90518602741457	33.25890131177226	27.00040005895606	13.835512601857117
7	77.04545933084876	5.874760491019729	12.795885709472977	4.283894468658539
8	78.68001600235824	5.189396332091045	12.25801697092203	3.8725706946286818
9	72.09230834017644	7.636599077758828	14.43791716674036	5.833175415324363
10	39.950097909165564	25.113385130440275	20.560608931842587	14.375908028551576
11	30.37068620638831	23.965110647884952	27.320131387783462	18.344071757943276
12	28.039184721959025	21.74973153938475	30.520181921546335	19.69090181710989
13	24.48476617606805	21.75099488345651	32.66649822079043	21.097740719685007
14	20.296148906154592	25.705261828058873	33.11319562882951	20.885393636957026
15	20.916029730697154	25.203187838207736	34.61194281262502	19.26883961847009
16	23.390289095235087	23.94237045459331	34.03101509696166	18.636325353209944
17	22.931484639841663	24.391068157412672	31.410313098772452	21.267134103973216
18	23.174573094982417	25.343208472827573	30.584086075842755	20.89813235634725
19	24.8888257216853	25.974564672688604	29.246309982523737	19.890299623102354
20	26.501589707956963	24.61941759838292	29.274419388120354	19.604573305539763
21	25.648727180847704	25.038953108879202	29.58783399658897	19.72448571368412
22	23.72907586381151	23.98679805445013	29.913566209757224	22.370559871981133
23	24.064072600172658	25.099172509633	29.938517255174446	20.898237635019896
24	23.334701956077737	24.261470111384835	30.579559092918956	21.824268839618473
25	24.164613732550063	24.854083759711955	29.09702482471101	21.884277683026973
26	22.852736192702082	26.465268565893922	29.39264733750237	21.28934790390163
27	23.369865032741668	25.460910028846357	30.053376287031774	21.115848651380205
28	22.472890741793528	26.371044153875307	30.220348261849118	20.935716842482048
29	23.2442675762744	25.358263323016022	29.919567094098078	21.4779020066115
30	22.981807845366685	24.38422504369065	30.094329690691257	22.539637420251406
31	23.412924009854084	25.429431705725055	28.320594613943108	22.837049670477754
32	23.743288484618784	26.38136146379467	28.77466152906744	21.10068852251911
33	23.475985934769334	26.255869286000046	28.23995115069589	22.02819362853473
34	22.50826437580275	26.9314425283726	28.71254711220601	21.847745983618637
35	22.939801654980734	25.404585938980485	28.3493356915756	23.306276714463184
36	22.43309540353315	28.596951129640157	26.638451982397406	22.331501484429282
37	23.606320931505696	28.059082391089213	26.369043859095026	21.965552818310066
38	22.924746804792285	27.256332512159688	27.161265870759905	22.65765481228813
39	23.62190217505738	25.27309287684501	27.26886067420462	23.836144273892995
40	23.504516455056535	25.13401975027899	29.45002421409471	21.91143958056977
41	21.21533699703114	26.968605899816815	28.842987387615015	22.973069715537026
42	22.242856842060934	27.82778514728486	27.61259554039543	22.316762470258773
43	23.06455688206683	25.8366496115217	28.547049038805717	22.551744467605754
44	23.050660097277493	26.11595393005285	27.858631798370286	22.97475417429937
45	22.876634451392835	25.13633588107721	27.816625607984335	24.170404059545618
46	21.878803192049354	26.352936222180112	27.732718505885078	24.03554207988546
47	23.313540942875793	25.749689427915694	28.514623207630596	22.422146421577914
48	22.183584949360956	25.183921841113428	28.98658749710484	23.645905712420777
49	21.882066830901394	25.807487419198623	28.583370180868762	23.727075569031225
50	20.487334975680625	25.387636072684394	30.12665024319373	23.998378708441244
51	21.13669382856421	24.908196997452254	29.065125386899123	24.889983787084415
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	477.0
1	953.0
2	1429.0
3	10955.0
4	20481.0
5	13670.5
6	6860.0
7	6615.5
8	6371.0
9	6231.5
10	6092.0
11	5873.5
12	5655.0
13	5557.0
14	5459.0
15	5050.0
16	4641.0
17	4469.0
18	4297.0
19	4164.0
20	4031.0
21	3901.0
22	3771.0
23	4139.0
24	4507.0
25	5382.5
26	6703.0
27	7148.0
28	9090.0
29	11032.0
30	12604.5
31	14177.0
32	15925.0
33	17673.0
34	18933.0
35	20193.0
36	22574.5
37	24956.0
38	26867.0
39	28778.0
40	33276.5
41	37775.0
42	40189.5
43	42604.0
44	47860.0
45	53116.0
46	60933.0
47	68750.0
48	72651.0
49	76552.0
50	75007.5
51	73463.0
52	67386.0
53	61309.0
54	58063.5
55	54818.0
56	52204.5
57	49591.0
58	49584.0
59	49577.0
60	49379.0
61	49181.0
62	45430.0
63	41679.0
64	36062.0
65	30445.0
66	25113.0
67	19781.0
68	16521.0
69	13261.0
70	11191.0
71	9121.0
72	7493.5
73	5866.0
74	4939.5
75	3226.5
76	2440.0
77	1809.0
78	1178.0
79	869.5
80	561.0
81	439.5
82	318.0
83	207.5
84	97.0
85	64.5
86	32.0
87	30.5
88	29.0
89	17.5
90	6.0
91	6.0
92	6.0
93	4.0
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	949860.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.621419647466595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.3912109436687	21.55478809799422
2	7.801273434523819	4.777721352463737
3	3.4719885218428255	3.1895165261560923
4	2.2621073466146995	2.7707575339322355
5	1.7439181269231818	2.6700624397669332
6	1.4736446757252952	2.7075055215983457
7	1.2912807225983152	2.767859422255683
8	1.198459946549316	2.935883596317359
9	1.0759411150178062	2.9652159959031033
>10	9.171862905580332	46.96428210008992
>50	0.07368576020457235	1.5234148894221238
>100	0.037707663425346385	2.1328411418600823
>500	0.004843186127995319	1.0184332342191913
>1k	0.0017297093314268999	0.7332634540529688
>5k	0.0	0.0
>10k+	3.4594186628537996E-4	1.2884546939679657
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12163	1.2805044953993219	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT	2157	0.22708609689849032	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT	1356	0.1427578801086476	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	1240	0.13054555408165414	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	1150	0.12107047354346957	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1019	0.10727896742677867	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	985	0.10369949255679785	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.263933632324763E-4	0.0	0.0	0.06116690880761375	0.0
2	5.263933632324763E-4	0.0	0.0	0.21540016423472932	0.0
3	5.263933632324763E-4	0.0	0.0	0.43174783652327714	0.0
4	5.263933632324763E-4	0.0	0.0	0.6957867475206873	0.0
5	5.263933632324763E-4	0.0	0.0	1.3989430021266291	0.0
6	5.263933632324763E-4	0.0	0.0	2.4135135704209043	0.0
7	5.263933632324763E-4	0.0	0.0	3.1216179226412315	0.0
8	5.263933632324763E-4	0.0	0.0	4.037437095993094	0.0
9	5.263933632324763E-4	0.0	0.0	4.6461583812351295	0.0
10	5.263933632324763E-4	0.0	0.0	5.410060429958099	0.0
11	5.263933632324763E-4	0.0	0.0	6.1898595582506895	0.0
12	5.263933632324763E-4	0.0	0.0	6.730991935653675	0.0
13	5.263933632324763E-4	0.0	0.0	7.019981892068305	0.0
14	7.369507085254669E-4	0.0	0.0	7.183374392015666	0.0
15	7.369507085254669E-4	0.0	0.0	7.368770134546144	0.0
16	7.369507085254669E-4	0.0	0.0	7.63712547112206	0.0
17	7.369507085254669E-4	0.0	0.0	7.938959425599562	0.0
18	7.369507085254669E-4	0.0	0.0	8.271745309835133	0.0
19	7.369507085254669E-4	0.0	0.0	8.499568357442149	0.0
20	7.369507085254669E-4	0.0	0.0	8.740551239129976	0.0
21	7.369507085254669E-4	0.0	0.0	9.020276672351715	0.0
22	7.369507085254669E-4	0.0	0.0	9.328743183205946	0.0
23	7.369507085254669E-4	0.0	0.0	9.645316151853958	0.0
24	7.369507085254669E-4	0.0	0.0	9.924831027730402	0.0
25	7.369507085254669E-4	0.0	0.0	10.171709515086434	0.0
26	8.422293811719622E-4	0.0	0.0	10.409639315267514	0.0
27	9.475080538184574E-4	0.0	0.0	10.643673804560672	0.0
28	0.0010527867264649526	0.0	0.0	10.892657865369634	0.0
29	0.001158065399111448	0.0	0.0	11.1475375318468	0.0
30	0.001158065399111448	0.0	0.0	11.453477354557513	0.0
31	0.001158065399111448	0.0	0.0	11.731518329016907	0.0
32	0.001158065399111448	0.0	0.0	12.032194218095299	0.0
33	0.001158065399111448	0.0	0.0	12.316446634240835	0.0
34	0.001158065399111448	0.0	0.0	12.585960036215864	0.0
35	0.001158065399111448	0.0	0.0	12.8656854694376	0.0
36	0.0012633440717579433	0.0	0.0	13.1690986040048	0.0
37	0.0012633440717579433	0.0	0.0	13.462720822015877	0.0
38	0.0012633440717579433	0.0	0.0	13.775714315793907	0.0
39	0.0012633440717579433	0.0	0.0	14.100604299580992	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTAT	20	7.032668E-4	45.0	10
ACGGTAC	20	7.032668E-4	45.0	13
TCACGAC	95	0.0	42.63158	25
CACGACG	95	0.0	42.63158	26
CGTTTTT	6825	0.0	42.461536	1
GGCGATA	325	0.0	42.23077	8
GCGATAT	60	3.6379788E-12	41.250004	9
CTATGCG	45	1.927765E-8	40.000004	1
CGAATAT	75	0.0	39.0	14
CGGTCTA	105	0.0	38.57143	31
CGACGGT	110	0.0	36.81818	28
TACGAGG	110	0.0	36.81818	2
GCGATAA	110	0.0	36.81818	9
GTTTTTT	8385	0.0	36.11807	2
TACGGTA	25	0.0021069248	36.0	29
GTATGCG	25	0.0021069248	36.0	1
TTCGGTA	25	0.0021069248	36.0	25
CGATACG	25	0.0021069248	36.0	12
CGAGGGA	500	0.0	35.55	4
TACGGGA	260	0.0	35.480766	4
>>END_MODULE