FastQCFastQC Report
Sat 18 Jun 2016
SRR3550541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341168
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6950.2037119542278291No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC5710.1673662242648783TruSeq Adapter, Index 14 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTC5040.14772780565586457No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC4790.14040003751817287TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT4090.11988228673263611TruSeq Adapter, Index 15 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT207.0263044E-445.0000046
GATCGAC207.0263044E-445.0000049
ATGTCGA207.0263044E-445.00000440
TCGACCA207.0263044E-445.00000443
TCGACAG207.0263044E-445.0000041
TTCGACG207.0263044E-445.00000425
ACGCGAG253.8850198E-545.01
AAGTACG253.8850198E-545.01
TTGTGCG253.8850198E-545.01
CGCGAGG551.8189894E-1245.02
CGTTCAT253.8850198E-545.017
TATTGCG253.8850198E-545.01
GTTCGGA253.8850198E-545.014
TTCGGAA253.8850198E-545.015
ACTCACC700.041.78571342
CGTTTTT4800.040.7812461
ACACGGG900.040.03
TACGGGA900.040.04
CGCACGG403.4486948E-739.3750042
ACGGGAA750.039.05