##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550541_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	341168
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.190835600056275	33.0	31.0	34.0	30.0	34.0
2	32.17953911269521	33.0	31.0	34.0	30.0	34.0
3	32.16416838624959	33.0	31.0	34.0	30.0	34.0
4	35.81443746189561	37.0	35.0	37.0	35.0	37.0
5	35.898955939595744	37.0	35.0	37.0	35.0	37.0
6	35.855877456267876	37.0	35.0	37.0	35.0	37.0
7	36.096140317966515	37.0	35.0	37.0	35.0	37.0
8	35.949206842376775	37.0	35.0	37.0	35.0	37.0
9	37.77564132626741	39.0	38.0	39.0	35.0	39.0
10	37.42395535337429	39.0	37.0	39.0	35.0	39.0
11	37.36745826103269	39.0	37.0	39.0	35.0	39.0
12	37.10994876424518	39.0	35.0	39.0	34.0	39.0
13	37.00961403179665	39.0	35.0	39.0	33.0	39.0
14	38.1140581766168	40.0	37.0	41.0	33.0	41.0
15	38.22123704450593	40.0	37.0	41.0	34.0	41.0
16	38.00684120433335	40.0	36.0	41.0	33.0	41.0
17	37.98498100642499	40.0	36.0	41.0	33.0	41.0
18	37.894415654457624	40.0	36.0	41.0	33.0	41.0
19	37.80951613281433	40.0	36.0	41.0	34.0	41.0
20	38.014271561224966	40.0	36.0	41.0	34.0	41.0
21	37.92166029639357	40.0	35.0	41.0	34.0	41.0
22	38.00016121089903	40.0	35.0	41.0	34.0	41.0
23	38.056107255076675	40.0	35.0	41.0	34.0	41.0
24	38.01711766636965	40.0	35.0	41.0	34.0	41.0
25	37.74402640341415	40.0	35.0	41.0	34.0	41.0
26	37.85666885522675	40.0	35.0	41.0	34.0	41.0
27	37.850865262861696	40.0	35.0	41.0	34.0	41.0
28	37.65000527599306	40.0	35.0	41.0	33.0	41.0
29	37.565264034141535	40.0	35.0	41.0	33.0	41.0
30	37.365148548515684	40.0	35.0	41.0	33.0	41.0
31	37.36525406837687	40.0	35.0	41.0	33.0	41.0
32	37.36695704169207	39.0	35.0	41.0	33.0	41.0
33	37.283514280354545	39.0	35.0	41.0	33.0	41.0
34	37.13224569713455	39.0	35.0	41.0	32.0	41.0
35	37.071706607888196	39.0	35.0	41.0	32.0	41.0
36	36.97277587581485	39.0	35.0	41.0	32.0	41.0
37	37.00007034657412	39.0	35.0	41.0	32.0	41.0
38	36.818121863715234	39.0	35.0	41.0	32.0	41.0
39	36.75418855226751	39.0	35.0	41.0	32.0	41.0
40	36.66660706748581	39.0	35.0	41.0	31.0	41.0
41	36.49944602072879	39.0	35.0	41.0	31.0	41.0
42	36.49507867091872	39.0	35.0	41.0	31.0	41.0
43	36.369252098672796	38.0	35.0	41.0	31.0	41.0
44	36.27183381794307	38.0	35.0	41.0	31.0	41.0
45	36.23608310275289	38.0	35.0	41.0	31.0	41.0
46	36.09450182901093	38.0	35.0	40.0	30.0	41.0
47	36.056520541199646	38.0	35.0	40.0	30.0	41.0
48	35.967174529850396	38.0	35.0	40.0	30.0	41.0
49	35.822155067298226	37.0	35.0	40.0	30.0	41.0
50	35.681804858603385	37.0	35.0	40.0	30.0	41.0
51	34.52152898278854	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	4.0
10	11.0
11	13.0
12	7.0
13	13.0
14	6.0
15	15.0
16	24.0
17	58.0
18	91.0
19	143.0
20	290.0
21	490.0
22	732.0
23	1006.0
24	1449.0
25	2011.0
26	2523.0
27	3186.0
28	3582.0
29	4016.0
30	4936.0
31	6175.0
32	7861.0
33	11740.0
34	25491.0
35	34598.0
36	23499.0
37	35628.0
38	63532.0
39	107978.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85894339445669	20.663719926839562	24.537764385874407	11.93957229282934
2	32.391959386577874	25.2063499507574	25.14479669840079	17.256893964263938
3	32.19117853960512	24.32144867044975	27.04561975331801	16.441753036627116
4	28.485086526286167	25.524375087933215	26.233409932936265	19.75712845284435
5	25.262627210054873	30.890060029076583	24.07377010739577	19.773542653472774
6	26.644937391549036	33.7229751911082	24.463021150869952	15.169066266472822
7	81.50852365989776	6.073840453969892	7.8272288139567605	4.5904070721755845
8	83.01364723537964	5.448342165736529	7.216679172724288	4.321331426159547
9	77.1866060122872	7.464357735778268	9.06151807906955	6.287518172864981
10	44.747162688177085	26.67131735684472	14.852213572199032	13.729306382779157
11	36.42868029826947	24.38300192280636	20.831672372555456	18.35664540636871
12	33.6315246447498	20.852190123340993	26.06838859447545	19.447896637433757
13	27.699842892651127	23.417202082258594	26.219926839562913	22.663028185527363
14	21.72478075317732	27.675221591708482	28.762076161890914	21.83792149322328
15	22.225413872344415	24.025699948412512	33.36567321671435	20.383212962528724
16	26.822269380481174	23.553791680345167	29.997537869905738	19.626401069267928
17	24.995603339117384	22.947931810720817	27.88098532101487	24.17547952914693
18	28.89251043474183	23.692726164235804	26.710594194062747	20.704169206959623
19	27.297988087980112	27.670531820100365	24.150565117478777	20.880914974440746
20	30.15786943675843	25.708448623552034	23.877092810580123	20.25658912910941
21	27.560908408760493	27.982108521315013	25.60556675889884	18.851416311025655
22	25.368440181963138	24.572058340758804	27.01484312713971	23.04465835013835
23	25.920367678094074	25.03751817286498	26.24718613703513	22.794928012005816
24	26.87268442526849	22.09175538151292	27.57761572011443	23.45794447310416
25	24.267809407681845	24.001371758195376	25.274351639075178	26.4564671950476
26	21.97509731276087	25.90453969891666	26.6513858275102	25.468977160812265
27	22.548421891853867	25.694379308727665	26.2269614969751	25.53023730244337
28	21.297132204661633	27.92348637621348	27.675221591708482	23.104159827416403
29	25.234488580406133	25.773519204614736	27.268090793978335	21.723901421000797
30	25.661257796745296	24.285689161937814	28.871699573230785	21.181353468086105
31	28.541070674858133	25.879332176523	24.20625615532524	21.373340993293628
32	28.9640294517657	25.62579139895887	25.13160671575294	20.278572433522488
33	27.05499929653426	25.11020963279088	25.46604605355719	22.368745017117668
34	24.856082633775735	25.471615157341837	28.665056511747878	21.00724569713455
35	23.33747596492051	27.16520892932514	28.070041738967312	21.427273366787038
36	23.545291469305447	30.28361393800122	25.281386296487362	20.889708296205974
37	25.647188481920928	29.401936875674156	24.227360127561788	20.723514514843128
38	24.390036580218542	28.954356797823944	23.636742015663835	23.018864606293672
39	27.59051259203677	24.91851521830887	24.315293345214087	23.175678844440277
40	26.664868920883556	22.68471837921493	26.73697415935844	23.913438540543076
41	21.927906485954136	25.47806359330301	26.05666416545514	26.537365755287716
42	23.85129906673545	24.681388641373164	25.46047694977255	26.00683534211884
43	23.765124513436195	23.91695586924917	25.297214275664775	27.020705341649865
44	22.856774375087934	24.40557144867045	25.30161093654739	27.436043239694225
45	24.568541012052712	24.066735449983586	24.9006354640529	26.4640880739108
46	24.2584298644656	26.139028279322794	25.66624067907893	23.936301177132673
47	22.48628241804624	24.813581578577125	26.952117431881067	25.748018571495567
48	22.811635323359752	23.06048632931576	29.45030014538292	24.677578201941568
49	24.802150260282325	22.774703371945787	28.76617971204802	23.656966655723867
50	22.027564132626743	23.524187497068894	29.19382826056371	25.254420109740654
51	21.53748299957792	23.292043802466818	27.98093607841298	27.18953711954228
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	141.0
1	392.0
2	643.0
3	1149.0
4	1655.0
5	1132.0
6	609.0
7	615.5
8	622.0
9	620.5
10	619.0
11	670.0
12	721.0
13	694.0
14	667.0
15	699.5
16	732.0
17	668.0
18	604.0
19	607.0
20	610.0
21	728.5
22	847.0
23	940.0
24	1033.0
25	1174.0
26	1610.5
27	1906.0
28	2338.5
29	2771.0
30	3171.5
31	3572.0
32	4363.0
33	5154.0
34	6022.5
35	6891.0
36	7035.0
37	7179.0
38	7945.0
39	8711.0
40	10185.5
41	11660.0
42	14067.0
43	16474.0
44	18282.5
45	20091.0
46	22728.0
47	25365.0
48	26930.0
49	28495.0
50	28505.5
51	28516.0
52	26729.5
53	24943.0
54	23649.5
55	22356.0
56	22100.5
57	21845.0
58	22018.5
59	22192.0
60	22202.0
61	22212.0
62	20082.5
63	17953.0
64	14839.0
65	11725.0
66	9685.5
67	7646.0
68	6170.0
69	4694.0
70	3847.0
71	3000.0
72	2606.0
73	2212.0
74	1644.5
75	927.0
76	777.0
77	630.0
78	483.0
79	357.5
80	232.0
81	182.5
82	133.0
83	92.0
84	51.0
85	34.0
86	17.0
87	12.5
88	8.0
89	6.5
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	341168.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.5478557016958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.02865122236398	21.39225132525158
2	9.25183235783802	5.652472796870317
3	3.348311061211119	3.0685116942680755
4	1.9735375117707226	2.4114935652582323
5	1.4986730298479212	2.2890623729908772
6	1.2461448682372567	2.284023217099227
7	1.143367886466294	2.4449206046807674
8	1.0296280066093626	2.5162342217862
9	1.0016921080035244	2.7539591375537236
>10	9.36841480939197	51.44380805274735
>50	0.07881839929572237	1.5961136941208505
>100	0.027935635193420585	1.607695195052031
>500	0.002993103770723634	0.5394541223207763
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	695	0.2037119542278291	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	571	0.1673662242648783	TruSeq Adapter, Index 14 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTC	504	0.14772780565586457	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	479	0.14040003751817287	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	409	0.11988228673263611	TruSeq Adapter, Index 15 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12721005487032783	0.0
2	0.0	0.0	0.0	0.5507550532289077	0.0
3	0.0	0.0	0.0	0.7802607513014116	0.0
4	0.0	0.0	0.0	1.0678023730244337	0.0
5	0.0	0.0	0.0	1.9661867467054355	0.0
6	0.0	0.0	0.0	2.544494208132064	0.0
7	0.0	0.0	0.0	3.0530413168878674	0.0
8	0.0	0.0	0.0	3.826267410777095	0.0
9	0.0	0.0	0.0	4.164810298738452	0.0
10	0.0	0.0	0.0	4.911656427331989	0.0
11	0.0	0.0	0.0	5.899732683018337	0.0
12	0.0	0.0	0.0	6.856446091075365	0.0
13	0.0	0.0	0.0	7.326888805515171	0.0
14	0.0	0.0	0.0	7.560498053744783	0.0
15	0.0	0.0	0.0	7.841591239506636	0.0
16	0.0	0.0	0.0	8.330793040378934	0.0
17	0.0	0.0	0.0	8.906755616001501	0.0
18	0.0	0.0	0.0	9.550133658490832	0.0
19	0.0	0.0	0.0	9.960781784927073	0.0
20	0.0	0.0	0.0	10.373774797167378	0.0
21	0.0	0.0	0.0	10.83366552548891	0.0
22	0.0	0.0	0.0	11.306160015007269	0.0
23	0.0	0.0	0.0	11.778361393800122	0.0
24	0.0	0.0	0.0	12.152663790273413	0.0
25	0.0	0.0	0.0	12.485637574450124	0.0
26	0.0	0.0	0.0	12.791058950429115	0.0
27	0.0	0.0	0.0	13.108790976879426	0.0
28	0.0	0.0	0.0	13.430919664212352	0.0
29	0.0	0.0	0.0	13.73927214744642	0.0
30	0.0	0.0	0.0	14.161058481451953	0.0
31	0.0	0.0	0.0	14.521877784551892	0.0
32	0.0	0.0	0.0	14.896473291750691	0.0
33	0.0	0.0	0.0	15.294224546264596	0.0
34	0.0	0.0	0.0	15.688165361346902	0.0
35	0.0	0.0	0.0	16.101451484312715	0.0
36	0.0	0.0	0.0	16.48278853819819	0.0
37	0.0	0.0	0.0	16.838918069690006	0.0
38	0.0	0.0	0.0	17.31111944848286	0.0
39	0.0	0.0	0.0	17.78859682033485	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	20	7.0263044E-4	45.000004	6
GATCGAC	20	7.0263044E-4	45.000004	9
ATGTCGA	20	7.0263044E-4	45.000004	40
TCGACCA	20	7.0263044E-4	45.000004	43
TCGACAG	20	7.0263044E-4	45.000004	1
TTCGACG	20	7.0263044E-4	45.000004	25
ACGCGAG	25	3.8850198E-5	45.0	1
AAGTACG	25	3.8850198E-5	45.0	1
TTGTGCG	25	3.8850198E-5	45.0	1
CGCGAGG	55	1.8189894E-12	45.0	2
CGTTCAT	25	3.8850198E-5	45.0	17
TATTGCG	25	3.8850198E-5	45.0	1
GTTCGGA	25	3.8850198E-5	45.0	14
TTCGGAA	25	3.8850198E-5	45.0	15
ACTCACC	70	0.0	41.785713	42
CGTTTTT	480	0.0	40.781246	1
ACACGGG	90	0.0	40.0	3
TACGGGA	90	0.0	40.0	4
CGCACGG	40	3.4486948E-7	39.375004	2
ACGGGAA	75	0.0	39.0	5
>>END_MODULE