FastQCFastQC Report
Sat 18 Jun 2016
SRR3550540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649483
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61640.9490625620685991No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT12670.19507823915329578No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT9640.14842574786407034No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT7270.11193518537051778Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC7210.11101137366182025TruSeq Adapter, Index 22 (95% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT6980.10747009544514637No Hit
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG6590.10146531933861241Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTCG207.0310174E-445.01
CCGGTAT207.0310174E-445.042
CGGTAAG207.0310174E-445.031
GGCGAAA207.0310174E-445.09
TACGTGA253.888926E-545.024
TAGTACG207.0310174E-445.01
TAATCGA207.0310174E-445.019
TACGCGG302.164039E-644.9999962
GTTAACG302.164039E-644.9999961
GTCAACG302.164039E-644.9999961
CGTTTTT31350.041.8421061
CGGTCTA700.041.78571731
TCGTTGA950.040.26315724
CGCATCG451.9261279E-840.00000421
CGACGGT800.039.37528
CATCGTT403.4551158E-739.37536
CTTACGG403.4551158E-739.3752
GGCTACG800.039.3751
TACGAAT1850.038.9189212
CGAATAT1850.038.9189214