Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550540_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 649483 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 6164 | 0.9490625620685991 | No Hit |
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT | 1267 | 0.19507823915329578 | No Hit |
CGTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT | 964 | 0.14842574786407034 | No Hit |
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT | 727 | 0.11193518537051778 | Illumina Single End Adapter 2 (95% over 23bp) |
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC | 721 | 0.11101137366182025 | TruSeq Adapter, Index 22 (95% over 24bp) |
CGTTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT | 698 | 0.10747009544514637 | No Hit |
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG | 659 | 0.10146531933861241 | Illumina Single End Adapter 2 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTATTCG | 20 | 7.0310174E-4 | 45.0 | 1 |
CCGGTAT | 20 | 7.0310174E-4 | 45.0 | 42 |
CGGTAAG | 20 | 7.0310174E-4 | 45.0 | 31 |
GGCGAAA | 20 | 7.0310174E-4 | 45.0 | 9 |
TACGTGA | 25 | 3.888926E-5 | 45.0 | 24 |
TAGTACG | 20 | 7.0310174E-4 | 45.0 | 1 |
TAATCGA | 20 | 7.0310174E-4 | 45.0 | 19 |
TACGCGG | 30 | 2.164039E-6 | 44.999996 | 2 |
GTTAACG | 30 | 2.164039E-6 | 44.999996 | 1 |
GTCAACG | 30 | 2.164039E-6 | 44.999996 | 1 |
CGTTTTT | 3135 | 0.0 | 41.842106 | 1 |
CGGTCTA | 70 | 0.0 | 41.785717 | 31 |
TCGTTGA | 95 | 0.0 | 40.263157 | 24 |
CGCATCG | 45 | 1.9261279E-8 | 40.000004 | 21 |
CGACGGT | 80 | 0.0 | 39.375 | 28 |
CATCGTT | 40 | 3.4551158E-7 | 39.375 | 36 |
CTTACGG | 40 | 3.4551158E-7 | 39.375 | 2 |
GGCTACG | 80 | 0.0 | 39.375 | 1 |
TACGAAT | 185 | 0.0 | 38.91892 | 12 |
CGAATAT | 185 | 0.0 | 38.91892 | 14 |