##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550540_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	649483
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18377694258356	33.0	31.0	34.0	30.0	34.0
2	32.211537484429925	33.0	31.0	34.0	30.0	34.0
3	32.17916712215716	33.0	31.0	34.0	30.0	34.0
4	35.85421481393662	37.0	35.0	37.0	35.0	37.0
5	35.94385842277627	37.0	35.0	37.0	35.0	37.0
6	35.90044389152603	37.0	35.0	37.0	35.0	37.0
7	36.168413953867926	37.0	35.0	37.0	35.0	37.0
8	35.988995862863234	37.0	35.0	37.0	35.0	37.0
9	37.853846828939325	39.0	38.0	39.0	35.0	39.0
10	37.49782981232765	39.0	37.0	39.0	35.0	39.0
11	37.46031227915126	39.0	37.0	39.0	35.0	39.0
12	37.4471910735154	39.0	37.0	39.0	35.0	39.0
13	37.464477130271305	39.0	37.0	39.0	35.0	39.0
14	38.66948480560692	40.0	38.0	41.0	35.0	41.0
15	38.77696105979679	40.0	38.0	41.0	35.0	41.0
16	38.523990620231785	40.0	38.0	41.0	34.0	41.0
17	38.596908618085465	40.0	38.0	41.0	35.0	41.0
18	38.477957082787384	40.0	38.0	41.0	34.0	41.0
19	38.27732365589246	40.0	37.0	41.0	34.0	41.0
20	38.40221376079128	40.0	37.0	41.0	34.0	41.0
21	38.30745069539926	40.0	37.0	41.0	34.0	41.0
22	38.40132228249238	40.0	37.0	41.0	35.0	41.0
23	38.4165805725477	40.0	37.0	41.0	35.0	41.0
24	38.341296077033576	40.0	37.0	41.0	34.0	41.0
25	38.07416822303278	40.0	36.0	41.0	34.0	41.0
26	38.157303578384656	40.0	37.0	41.0	34.0	41.0
27	38.177993881287115	40.0	37.0	41.0	34.0	41.0
28	38.09193312219104	40.0	37.0	41.0	34.0	41.0
29	38.06565683782331	40.0	37.0	41.0	34.0	41.0
30	37.87983981104971	40.0	36.0	41.0	34.0	41.0
31	37.815996723547805	40.0	36.0	41.0	33.0	41.0
32	37.696255329238795	40.0	36.0	41.0	33.0	41.0
33	37.53583696570965	40.0	36.0	41.0	33.0	41.0
34	37.391633037354325	40.0	36.0	41.0	33.0	41.0
35	37.267483521508645	40.0	36.0	41.0	33.0	41.0
36	37.10830922441388	40.0	35.0	41.0	32.0	41.0
37	37.049299211834644	40.0	35.0	41.0	32.0	41.0
38	36.92519896594676	40.0	35.0	41.0	31.0	41.0
39	36.84669190725546	40.0	35.0	41.0	31.0	41.0
40	36.760869799517465	40.0	35.0	41.0	31.0	41.0
41	36.63907446384278	39.0	35.0	41.0	31.0	41.0
42	36.59520110611055	39.0	35.0	41.0	31.0	41.0
43	36.52172728154547	39.0	35.0	41.0	31.0	41.0
44	36.40130996500293	39.0	35.0	41.0	30.0	41.0
45	36.363381335616175	39.0	35.0	41.0	30.0	41.0
46	36.219183566005576	39.0	35.0	41.0	30.0	41.0
47	36.15373150644436	39.0	35.0	41.0	30.0	41.0
48	36.04343300748442	39.0	35.0	41.0	30.0	41.0
49	35.98781954262083	39.0	35.0	41.0	29.0	41.0
50	35.88648817597997	39.0	35.0	41.0	29.0	41.0
51	34.73657663095108	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	5.0
9	13.0
10	15.0
11	19.0
12	9.0
13	19.0
14	20.0
15	31.0
16	49.0
17	80.0
18	148.0
19	262.0
20	464.0
21	689.0
22	1150.0
23	1852.0
24	2921.0
25	4635.0
26	6520.0
27	7553.0
28	7353.0
29	7868.0
30	8448.0
31	10294.0
32	12889.0
33	17686.0
34	32937.0
35	46791.0
36	46153.0
37	69558.0
38	128020.0
39	234857.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.32068583781253	21.026262427192087	24.56954223590148	14.083509499093893
2	34.56980398255228	24.03403938209314	24.966011427550836	16.430145207803744
3	31.0720988848053	23.102991148344145	29.750740204131592	16.074169762718963
4	27.859235730573396	25.3672536463618	28.774425196656416	17.99908542640839
5	24.76893159636203	30.328738396540018	26.737266410360238	18.165063596737713
6	25.910146993839717	34.39982262815193	25.444545892656155	14.245484485352195
7	79.19668413184024	6.059127028729004	10.498812132111233	4.245376707319514
8	80.34082493306214	5.594757676490378	10.133136664085127	3.931280726362353
9	73.68383776018771	8.068263526528023	12.459756452439864	5.788142260844395
10	38.114161571588475	29.45973951589187	18.60356622113281	13.82253269138684
11	27.876326247184295	24.231735087754412	28.878200045266773	19.013738619794513
12	26.138482454506125	22.077868088926113	31.411291750515407	20.37235770605235
13	23.348879031475807	23.35981080336206	32.739117113149995	20.55219305201214
14	20.023156880164684	26.84950953296699	31.639781179799932	21.487552407068392
15	20.264117767516623	25.379725104429212	34.34808917246487	20.008067955589294
16	22.546086656617646	24.99095434368567	31.97512483005714	20.487834169639545
17	21.919588349502604	25.588660519212976	29.954594654517518	22.537156476766906
18	22.86218422961032	25.645782876534106	30.345828913150925	21.14620398070465
19	23.721483087317143	26.63025822076944	28.626615323264815	21.0216433686486
20	25.131681660643928	25.596820855973135	29.138099072647012	20.13339841073592
21	24.500256357749166	26.608702614233167	28.88713022511752	20.00391080290015
22	23.438026861365117	24.433280008868593	28.76087595826219	23.367817171504104
23	22.450318176149338	26.447189533829214	29.140716539154987	21.961775750866458
24	22.66233296329542	25.34046310680957	30.382781381498823	21.61442254839619
25	22.435537188810176	26.938657362856304	28.277106560140915	22.348698888192608
26	21.512341354585107	27.862623040171954	28.35455277505339	22.27048283018955
27	22.486192864170423	26.74481087264794	29.016925770189523	21.752070492992118
28	21.114794382608938	26.728798136363846	30.234355633634753	21.922051847392467
29	22.05923788613405	25.105506995564163	29.97753597861684	22.85771913968495
30	23.01430522430918	25.747864070345184	29.44911568124185	21.788715024103787
31	24.231735087754412	26.74804421362838	26.90247473759898	22.117745961018226
32	23.74011329010921	26.951282789541835	27.44044108929718	21.868162831051773
33	24.04620290292433	26.440106977395867	26.889849310913448	22.623840808766357
34	22.245539914054717	25.923696232233944	28.69667104450771	23.134092809203626
35	23.685146493441707	24.970476517476207	27.92236286400106	23.422014125081027
36	23.779683224965087	27.21010403659526	26.73603466141531	22.274178077024338
37	22.896673200068363	27.777632362971776	28.007045603964997	21.31864883299486
38	23.19614216230448	27.370847273908634	26.832880922210435	22.600129641576455
39	22.845555618853766	25.811145172390965	27.603955761736643	23.73934344701863
40	23.93057247071902	25.143383275620764	29.29314547109008	21.632898782570138
41	22.053695015881864	25.123983229738116	28.864034932400077	23.958286821979943
42	22.66063930849614	25.81822772882431	27.764545030431897	23.75658793224765
43	22.529150108624858	24.982024163834925	28.716225059008472	23.77260066853174
44	22.662794869149767	25.4600967230859	27.74499101593113	24.132117391833198
45	22.666490115984562	25.375875888976307	27.932832730032963	24.024801265006165
46	22.821228577191395	26.1287824315648	27.93498829068659	23.11500070055721
47	22.500050039800886	25.76141330873941	29.100068823972297	22.638467827487403
48	22.20227473236405	24.82697776539186	29.318396324461148	23.652351177782943
49	22.081563335760908	25.322140841253738	29.149800687623852	23.446495135361513
50	21.515420726947436	24.308257490958194	29.64034470494224	24.535977077152136
51	21.466150769150232	24.489016648626677	28.463254619443468	25.58157796277963
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	269.0
1	622.0
2	975.0
3	5223.0
4	9471.0
5	6222.5
6	2974.0
7	2925.5
8	2877.0
9	2855.5
10	2834.0
11	2887.0
12	2940.0
13	2873.0
14	2806.0
15	2684.0
16	2562.0
17	2506.5
18	2451.0
19	2525.5
20	2600.0
21	2464.0
22	2328.0
23	2484.0
24	2640.0
25	3116.5
26	4470.5
27	5348.0
28	6521.0
29	7694.0
30	9297.5
31	10901.0
32	11957.5
33	13014.0
34	15592.0
35	18170.0
36	18815.5
37	19461.0
38	20934.5
39	22408.0
40	25489.5
41	28571.0
42	30548.0
43	32525.0
44	35587.5
45	38650.0
46	41480.0
47	44310.0
48	50276.5
49	56243.0
50	57031.0
51	57819.0
52	55491.5
53	53164.0
54	46926.5
55	40689.0
56	36661.5
57	32634.0
58	30464.5
59	28295.0
60	27100.5
61	25906.0
62	23380.0
63	20854.0
64	18321.5
65	15789.0
66	13844.5
67	11900.0
68	10150.0
69	8400.0
70	7363.5
71	6327.0
72	5174.0
73	4021.0
74	3149.5
75	1821.0
76	1364.0
77	985.5
78	607.0
79	515.0
80	423.0
81	313.5
82	204.0
83	150.0
84	96.0
85	68.0
86	40.0
87	35.5
88	31.0
89	23.5
90	16.0
91	11.5
92	7.0
93	5.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	649483.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.29939472057807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.94005990920235	23.55919785949939
2	8.680500935174674	5.6074985215510775
3	3.300361939343768	3.1979907899891074
4	1.9931819099002237	2.5751427703113206
5	1.3952196639895897	2.2532375324556035
6	1.123133491574096	2.1765919180951663
7	0.9447330419234904	2.1360013798661472
8	0.8113988054698059	2.0966152234939845
9	0.7534052110883326	2.190107906773425
>10	7.925795205428085	47.83388491628774
>50	0.08909796726312746	1.9333934906489232
>100	0.03832170634880967	2.4390216858206473
>500	0.003832170634880967	0.8515843287716851
>1k	4.790213293601209E-4	0.19603149428665062
>5k	4.790213293601209E-4	0.9537001821491037
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6164	0.9490625620685991	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1267	0.19507823915329578	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT	964	0.14842574786407034	No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	727	0.11193518537051778	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	721	0.11101137366182025	TruSeq Adapter, Index 22 (95% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT	698	0.10747009544514637	No Hit
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	659	0.10146531933861241	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.079372362325111E-4	0.0	0.0	0.12024949074879558	0.0
2	3.079372362325111E-4	0.0	0.0	0.4207962333117264	0.0
3	3.079372362325111E-4	0.0	0.0	0.6779238255658732	0.0
4	3.079372362325111E-4	0.0	0.0	1.0275865573078895	0.0
5	3.079372362325111E-4	0.0	0.0	1.9313823456503096	0.0
6	3.079372362325111E-4	0.0	0.0	2.7828288038332025	0.0
7	3.079372362325111E-4	0.0	0.0	3.5129479909404866	0.0
8	3.079372362325111E-4	0.0	0.0	4.573791769761487	0.0
9	3.079372362325111E-4	0.0	0.0	5.113605744877079	0.0
10	3.079372362325111E-4	0.0	0.0	6.007393573041942	0.0
11	3.079372362325111E-4	0.0	0.0	7.023278515372997	0.0
12	3.079372362325111E-4	0.0	0.0	7.897974234891445	0.0
13	3.079372362325111E-4	0.0	0.0	8.268884635933505	0.0
14	3.079372362325111E-4	0.0	0.0	8.456110475562872	0.0
15	3.079372362325111E-4	0.0	0.0	8.701690421458299	0.0
16	3.079372362325111E-4	0.0	0.0	9.130185085675837	0.0
17	3.079372362325111E-4	0.0	0.0	9.701870564741494	0.0
18	3.079372362325111E-4	0.0	0.0	10.307429139792728	0.0
19	3.079372362325111E-4	0.0	0.0	10.698201492571783	0.0
20	3.079372362325111E-4	0.0	0.0	11.079889696881981	0.0
21	3.079372362325111E-4	0.0	0.0	11.525782814946657	0.0
22	3.079372362325111E-4	0.0	0.0	12.048968179305694	0.0
23	3.079372362325111E-4	0.0	0.0	12.537818541824805	0.0
24	3.079372362325111E-4	0.0	0.0	12.93890679201765	0.0
25	3.079372362325111E-4	0.0	0.0	13.316899749493059	0.0
26	4.619058543487666E-4	0.0	0.0	13.66225135992782	0.0
27	4.619058543487666E-4	0.0	0.0	14.023307769410438	0.0
28	4.619058543487666E-4	0.0	0.0	14.379591151731454	0.0
29	4.619058543487666E-4	0.0	0.0	14.77529050029023	0.0
30	4.619058543487666E-4	0.0	0.0	15.235348731221602	0.0
31	4.619058543487666E-4	0.0	0.0	15.62565917814631	0.0
32	4.619058543487666E-4	0.0	0.0	16.032444267209456	0.0
33	4.619058543487666E-4	0.0	0.0	16.419059467299373	0.0
34	4.619058543487666E-4	0.0	0.0	16.777190473037784	0.0
35	4.619058543487666E-4	0.0	0.0	17.182589844537887	0.0
36	4.619058543487666E-4	0.0	0.0	17.58260031440392	0.0
37	4.619058543487666E-4	0.0	0.0	17.96659804798586	0.0
38	4.619058543487666E-4	0.0	0.0	18.349979907095335	0.0
39	4.619058543487666E-4	0.0	0.0	18.748296722162088	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTCG	20	7.0310174E-4	45.0	1
CCGGTAT	20	7.0310174E-4	45.0	42
CGGTAAG	20	7.0310174E-4	45.0	31
GGCGAAA	20	7.0310174E-4	45.0	9
TACGTGA	25	3.888926E-5	45.0	24
TAGTACG	20	7.0310174E-4	45.0	1
TAATCGA	20	7.0310174E-4	45.0	19
TACGCGG	30	2.164039E-6	44.999996	2
GTTAACG	30	2.164039E-6	44.999996	1
GTCAACG	30	2.164039E-6	44.999996	1
CGTTTTT	3135	0.0	41.842106	1
CGGTCTA	70	0.0	41.785717	31
TCGTTGA	95	0.0	40.263157	24
CGCATCG	45	1.9261279E-8	40.000004	21
CGACGGT	80	0.0	39.375	28
CATCGTT	40	3.4551158E-7	39.375	36
CTTACGG	40	3.4551158E-7	39.375	2
GGCTACG	80	0.0	39.375	1
TACGAAT	185	0.0	38.91892	12
CGAATAT	185	0.0	38.91892	14
>>END_MODULE