FastQCFastQC Report
Sat 18 Jun 2016
SRR3550538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605363
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29650.48978877136528No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8050.1329780644010288No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC6460.1067128318050492Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA253.8886104E-545.038
TTTCGCG207.0306385E-445.01
CATGACG207.0306385E-445.01
TAACGCC207.0306385E-445.012
CTTGACG207.0306385E-445.01
TGTAGCG406.8066583E-945.01
TAGTGCG406.8066583E-945.01
TGCGTTG207.0306385E-445.01
CGACTGG302.1637934E-644.9999962
CGTTTTT14950.039.4314351
GATTACG403.4546065E-739.3751
CGGGTAT750.039.06
GCTACGA1050.038.5714310
CGCGGGT356.24339E-638.5714264
CCGCAGG356.24339E-638.5714262
ACGGGTA659.094947E-1238.0769275
CACGACA301.1392839E-437.49999634
TAACGAG301.1392839E-437.4999961
ATGCGAG301.1392839E-437.4999961
TATCGCG301.1392839E-437.4999961